To investigate the impact of different milling parameters on milling forces, chip morphology, and machined surface quality during the Paulownia milling process, we conducted experiments using cemented carbide single-tooth shank milling cutters. Additionally, we established a response surface model (RSM) to analyze milling surface quality. The key findings are as follows: milling forces along the parallel and tangential axes decrease with an increased tool rake angle and spindle’s rotational frequency, but they exhibit a positive correlation with milling depth. The effect of spindle’s rotational frequency on the milling force along the lateral axis differs due to the complex fiber characteristics of Paulownia. As milling depth decreases, chip morphology transitions from a block structure to a sheet structure, eventually becoming fragmented with shallow milling. Higher spindle’s rotational frequency and tool rake angle lead to a more fragmented direction in Paulownia chip morphology, while machined surface quality improves. Notably, under specific conditions, a striped chip morphology significantly enhances machined surface quality compared to similar milling parameters. The established RSM for machined Paulownia surface roughness is reliable and holds reference value for inhibiting surface damage in Paulownia machining.