Force Jumps Research Articles

Simulation of developing process of forced hydraulic jumps by an explicit incompressible particle method

Simulation of developing process of forced hydraulic jumps by an explicit incompressible particle method

Catch bonds in sickle cell disease: Shear-enhanced adhesion of red blood cells to laminin

Could the phenomenon of catch bonding—force-strengthened cellular adhesion—play a role in sickle cell disease, where abnormal red blood cell (RBC) adhesion obstructs blood flow? Here, we investigate the dynamics of sickle RBCs adhering to a surface functionalized with the protein laminin (a component of the extracellular matrix around blood vessels) under physiologically relevant microscale flow. First, using total internal reflectance microscopy we characterize the spatial fluctuations of the RBC membrane above the laminin surface before detachment. The complex dynamics we observe suggest the possibility of catch bonding, where the mean detachment time of the cell from the surface initially increases to a maximum and then decreases as a function of shear force. We next conduct a series of shear-induced detachment experiments on blood samples from 25 sickle cell disease patients, quantifying the number and duration of adhered cells under both sudden force jumps and linear force ramps. The experiments reveal that a subset of patients does indeed exhibit catch bonding. By fitting the data to a theoretical model of the bond dynamics, we can extract the mean bond lifetime versus force for each patient. The results show a striking heterogeneity among patients, both in terms of the qualitative behavior (whether or not there is catch bonding) and in the magnitudes of the lifetimes. Patients with large bond lifetimes at physiological forces are more likely to have certain adverse clinical features, like a diagnosis of pulmonary arterial hypertension and intracardiac shunts. By introducing an in vitro platform for fully characterizing RBC-laminin adhesion dynamics, our approach could contribute to the development of patient-specific antiadhesive therapies for sickle cell disease. The experimental setup is also easily generalizable to studying adhesion dynamics in other cell types, for example, leukocytes or cancer cells, and can incorporate disease-relevant environmental conditions like oxygen deprivation.

Experimental study of shear deformation of the medium formed by the massif of ribbed grains

The approach to the study of the seismically active zone as an open system that is in a state of self-organized criticality is becoming increasingly used in modern research. The models used in this approach should reflect the most characteristic features of such media: discreteness, nonequilibrium, nonlinear and nonlocal nature of interactions. In this paper, the medium is modeled by a granular massif with ribbed grains. Three different massifs are used in the research. Within one massif, all grains are identical, while grains from different massifs differ in shape (cubic or irregular ribbed) and characteristic size.
 A number of experiments of shear deformation of such granular massifs were carried out in order to study the influence of the shape and size of grains on the statistical charac-teristics of the process. The influence of the stress state on the deformation properties of these media was also studied. The experiments proved the qualitative similarity of the behavior of different granular media. Obtained experimentally force jumps, represen-ting the reaction of the granular medium to shear deformation, obey the distributions in the form of power dependencies. However, the magnitudes of the forces arising in the massifs depend on the grain shape and size as well as the stress state in the massifs. The experiments with shear deformation of the granular media under the external action of small impulse stresses have shown that such action causes a smoother, devoid of sharp jumps, deformation. External perturbations shift the distribution of the force jumps towards smaller values, leaving their exponents unchanged.
 The analysis of experimental results using nonadditive statistical mechanics confirmed the presence of long-range correlations in the massif of ribbed granules during its shear deformation.

Mechanochemical properties of DNA origami nanosprings revealed by force jumps in optical tweezers.

By incorporating pH responsive i-motif elements, we have constructed DNA origami nanosprings that respond to pH changes in the environment. Using an innovative force jump approach in optical tweezers, we have directly measured the spring constants and dynamic recoiling responses of the DNA nanosprings under different forces. These DNA nanosprings exhibited 3 times slower recoiling rates compared to duplex DNA backbones. In addition, we observed two distinct force regions which show different spring constants. In the entropic region below 2 pN, a spring constant of ∼0.03 pN nm-1 was obtained, whereas in the enthalpic region above 2 pN, the nanospring was 17 times stronger (0.5 pN nm-1). The force jump gave a more accurate measurement on nanospring constants compared to regular force ramping approaches, which only yielded an average spring constant in a specific force range. Compared to the reported DNA origami nanosprings with a completely different design, our nanospring is up to 50 times stiffer. The drastic increase in the spring constant and the pH responsive feature allow more robust applications of these nanosprings in many mechanobiological processes.

Different Barbell Height Positions Affect Maximal Isometric Deadlift Force and Subsequent Squat Jump Performance in Recreationally-Trained Men

Different Barbell Height Positions Affect Maximal Isometric Deadlift Force and Subsequent Squat Jump Performance in Recreationally-Trained Men

The Effect of Height on Drop Jumps in Relation to Somatic Parameters and Landing Kinetics.

The aim of this study was to assess the effect of drop height and selected somatic parameters on the landing kinetics of rebound jumps in force and power production, performed by male and female student athletes. Twenty female and forty male students with a sports background participated in the experiment (mean and standard deviation (± SD): age 20.28 ± 1.31 years, height 166.78 ± 5.29 cm, mass 62.23 ± 7.21 kg and 21.18 ± 1.29, 182.18 ± 6.43, 78.65 ± 7.09). Each participant performed three maximal jumps on two independent and synchronized force platforms (Bilateral Tensiometric Platform S2P) at each of the two assigned drop-jump heights (20-, and 40-, cm for female and 30-, and 60-, cm for the male special platform). Significant between-sex differences were observed in all variables of selected somatics, with men outperforming women. Statistically significant differences were noted in four parameters, between men and women, in both DJs from 20/40 and 30/60 cm. The height of the jump was 6 cm and 4 cm higher for men. A slightly higher statistical significance (p = 0.011) was demonstrated by the relative strength (% BW) generated by the left limb in both men and women. Only women showed a significant relationship between body mass, body height, and five parameters, dropping off of a 20 cm box. In men, only the left leg—relative maximal F (p =−0.45)—showed a relationship with body mass. There were no relationships between the above-mentioned dependencies in both groups, in jumps from a higher height: 40 cm and 60 cm. From a practical application, the DJ with lower 20/30 cm or higher 40/60 cm (women/men) respectively emphasizes either the force or power output via an increase in the velocity component of the rebound action or increased height of the DJ jump.

Carry-over effects of the larval environment on the post-metamorphic performance of Bombina variegata (Amphibia, Anura)

Metamorphs of the yellow-bellied toad, Bombina variegata, vary widely in size at metamorphosis in the field. We performed a replicated outdoor mesocosm study to simulate the environmental factor combinations in permanent and ephemeral breeding sites and to quantify their effects on tadpole development (duration of the larval period, metamorph size and body condition). Looking for potential carry-over effects of the larval environment, we quantified locomotor performance of all metamorph phenotypes originating from the mesocosms immediately after metamorphosis under controlled conditions. In contrast to the prediction of life-history theory, tadpoles were unable to adjust developmental rate to water availability, but metamorphs originating from the ephemeral pond treatment were smaller and had a lower body condition than those from the permanent pond treatment. Size-dependent carry-over effects included the length of the first jump following tactile stimulation, burst performance (total length of spontaneous jumps) and endurance (total distance covered in 10 forced jumps). A size-independent effect of larval environment was the prolonged locomotor effort to escape (5.7 consecutive jumps following initial stimulus) of metamorphs from the ephemeral pond treatment compared to same-sized ones (3.7 jumps) from the permanent pond treatment. Thus, we demonstrate that carry-over effects of larval environment on metamorph phenotype and behaviour cause a considerable variation in fitness in the early terrestrial stage of B. variegata. Informed conservation management of endangered populations in the northern range should therefore include the provision of small permanent breeding ponds promoting larger and fitter metamorphs.

Fundamental Characteristics of Neuron Adhesion Revealed by Forced Peeling and Time-Dependent Healing

A current bottleneck in the advance of neurophysics is the lack of reliable methods to quantitatively measure the interactions between neural cells and their microenvironment. Here, we present an experimental technique to probe the fundamental characteristics of neuron adhesion through repeated peeling of well-developed neurite branches on a substrate with an atomic force microscopy cantilever. At the same time, a total internal reflection fluorescence microscope is also used to monitor the activities of neural cell adhesion molecules (NCAMs) during detaching. It was found that NCAMs aggregate into clusters at the neurite-substrate interface, resulting in strong local attachment with an adhesion energy of ∼0.1 mJ/m2 and sudden force jumps in the recorded force-displacement curve. Furthermore, by introducing a healing period between two forced peelings, we showed that stable neurite-substrate attachment can be re-established in 2–5 min. These findings are rationalized by a stochastic model, accounting for the breakage and rebinding of NCAM-based molecular bonds along the interface, and provide new insights into the mechanics of neuron adhesion as well as many related biological processes including axon outgrowth and nerve regeneration.

Measurement Characteristics of Athlete Monitoring Tools in Professional Australian Football.

To examine the measurement reliability and sensitivity of common athlete monitoring tools in professional Australian Football players. Test-retest reliability (noise) and weekly variation (signal) data were collected from 42 professional Australian footballers from 1 club during a competition season. Perceptual wellness was measured via questionnaires completed before main training sessions (48, 72, and 96h postmatch), with players providing a rating (1-5 Likert scale) regarding their muscle soreness, sleep quality, fatigue level, stress, and motivation. Eccentric hamstring force and countermovement jumps were assessed via proprietary systems once per week. Heart rate recovery was assessed via a standard submaximal run test on a grass-covered field with players wearing a heart rate monitor. The heart rate recovery was calculated by subtracting average heart rate during final 10 seconds of rest from average heart rate during final 30 seconds of exercise. Typical test error was reported as coefficient of variation percentage (CV%) and intraclass coefficients. Sensitivity was calculated by dividing weekly CV% by test CV% to produce a signal to noise ratio. All measures displayed acceptable sensitivity. Signal to noise ratio ranged from 1.3 to 11.1. Intraclass coefficients ranged from .30 to .97 for all measures. The heart rate recovery test, countermovement jump test, eccentric hamstring force test, and perceptual wellness all possess acceptable measurement sensitivity. Signal to noise ratio analysis is a novel method of assessing measurement characteristics of monitoring tools. These data can be used by coaches and scientists to identify meaningful changes in common measures of fitness and fatigue in professional Australian football.

Muscle oxygenation induced by cycling exercise does not accelerate recovery kinetics following exercise-induced muscle damage in humans: A randomized cross-over study

The aim of this study was to analyze the effects of inducing muscle oxygenation using an intermittent cycling exercise on recovery kinetics after exercise-induced muscle damage. Ten soccer players performed single-leg knee flexors exercise: 75 eccentric contractions. The day after, subjects performed an intermittent cycling exercise of 12 min (15 s work - 15 s rest) or recovered passively in a balanced and randomized cross-over design. Force, single and double-leg countermovement jumps, muscle soreness, perceived recovery and creatine kinase concentrations were assessed through a 72 h period. Oxygenation during cycling was assessed using Near Infrared Spectroscopy. Results showed an increase in knee flexors oxygenation using intermittent cycling (ΔHbO2 = 70.2 ± 19.8% ; ΔHHb = 68.2 ± 14.1%). Possibly small detrimental effect of cycling on eccentric force was found (ES = -0.58, 90% CI: -1.33 to 0.17). Small detrimental effects of cycling were found for soreness and perceived recovery. Implementing intermittent cycling exercise the day after muscle damage may be detrimental for recovery.

Maximum Strength, Rate of Force Development, Jump Height, and Peak Power Alterations in Weightlifters across Five Months of Training

The purpose of this monitoring study was to investigate how alterations in training affect changes in force-related characteristics and weightlifting performance. Subjects: Seven competitive weightlifters participated in the study. Methods: The weightlifters performed a block style periodized plan across 20 weeks. Force plate data from the isometric mid-thigh pull and static jumps with 0 kg, 11 kg, and 20 kg were collected near the end of each training block (weeks 1, 6, 10, 13, 17, and 20). Weightlifting performance was measured at weeks 0, 7, 11, and 20. Results: Very strong correlations were noted between weightlifting performances and isometric rate of force development (RFD), isometric peak force (PF), peak power (PP), and jump height (JH). Men responded in a more predictable manner than the women. During periods of higher training volume, RFD was depressed to a greater extent than PF. JH at 20 kg responded in a manner reflecting the expected fatigue response more so than JH at 0 kg and 11 kg. Conclusions: PF appears to have been more resistant to volume alterations than RFD and JH at 20 kg. RFD and JH at 20 kg appear to be superior monitoring metrics due to their “sensitivity.”

Forced Hydraulic Jumps Described by Classic Hydraulic Equations Reproducing Cusp Catastrophe Features

The information content of the classic equations describing the problem of forced hydraulic jumps in open channels is the subject of this paper. The forcing refers to designed structural composites to transform incoming supercritical flows into outgoing subcritical flows through hydraulic jumps. The complete flow history through such systems is described by two stable flow profiles, which are interlocked and one can jump to the other. The interlocked profiles underpin a hydraulic effect known as hysteresis with the prime feature of the dependence of flows on their past history. The paper develops an algorithm by using classic hydraulic equations alone to identify hysteresis by building on tacit knowledge already published. The algorithm reproduces the effects of catastrophe theory and shows that hysteresis has predictable configurations. It is validated for geometries with step-rises by using published experimental data; as well as a flume with abrupt contractions by authors’ experimental data.

Particle Image Velocity Measurement and Mesh-Free Method Modeling Study of Forced Hydraulic Jumps

AbstractThe current study aims at examining the internal flow field and the corresponding phenomena in the developing stage of forced hydraulic jumps. Experiments with a range of Froude numbers wer...

Relationship between vertical and horizontal jump variables and muscular performance in athletes.

This study investigated the relationship between vertical and horizontal measures in bilateral and unilateral countermovement jump, drop jump and squat jump (SJ), and sprinting speed and muscle architecture of both the vastus lateralis and gastrocnemius. Subjects (n = 17) completed a 30-m sprint test, muscle stiffness test; ultrasound measures, and a jump testing session. Measures of horizontal peak and mean force, in both bilateral and unilateral jumps, tended to have greater relationships to sprint speeds (R = 0.132-0.576) than peak and mean force in the vertical plane (R = 0.008-0.504). Vertical velocity variables also showed some large and very large correlations to sprint speed (R = 0.062-0.635). Unilateral measures of velocity tended to have larger correlations to sprint performance than their bilateral counterparts across all jump types and peak and mean velocity in SJ showed large and very large correlations to sprint speed (bilateral R = 0.227-0.635; unilateral 0.393-0.574). Few large correlations were shown between muscle stiffness measures of muscle architecture and kinetic and kinematic variables in either vertical or horizontal jumps. The present findings suggest that sport scientists and strength and conditioning practitioners concerned with the prognostic value of kinetic variables to functional movements such as sprint speed should also use horizontal jumps in addition to vertical jumps in testing and training.

Instant effects of peppermint essential oil on the physiological parameters and exercise performance.

Effect of peppermint on exercise performance was previously investigated but equivocal findings exist. This study aimed to investigate the effects of peppermint ingestion on the physiological parameters and exercise performance after 5 min and 1 h. Thirty healthy male university students were randomly divided into experimental (n=15) and control (n=15) groups. Maximum isometric grip force, vertical and long jumps, spirometric parameters, visual and audio reaction times, blood pressure, heart rate, and breath rate were recorded three times: before, five minutes, and one hour after single dose oral administration of peppermint essential oil (50 µl). Data were analyzed using repeated measures ANOVA. Our results revealed significant improvement in all of the variables after oral administration of peppermint essential oil. Experimental group compared with control group showed an incremental and a significant increase in the grip force (36.1%), standing vertical jump (7.0%), and standing long jump (6.4%). Data obtained from the experimental group after five minutes exhibited a significant increase in the forced vital capacity in first second (FVC1)(35.1%), peak inspiratory flow rate (PIF) (66.4%), and peak expiratory flow rate (PEF) (65.1%), whereas after one hour, only PIF shown a significant increase as compare with the baseline and control group. At both times, visual and audio reaction times were significantly decreased. Physiological parameters were also significantly improved after five minutes. A considerable enhancement in the grip force, spiromery, and other parameters were the important findings of this study. Conclusion : An improvement in the spirometric measurements (FVC1, PEF, and PIF) might be due to the peppermint effects on the bronchial smooth muscle tonicity with or without affecting the lung surfactant. Yet, no scientific evidence exists regarding isometric force enhancement in this novel study.

Mechanically unzipping dsDNA with built-in sequence inhomogeneities and bound proteins

We theoretically analyze the force signal expected during unzipping through a single double-stranded DNA (dsDNA) molecule with designed subsequences or inhomogeneities with large stability differentials compared to the rest of the molecule. Our calculations describe experiments where the extension between the unzipped ends is fixed--the so-called fixed-extension ensemble--and the equilibrium force is measured. Two different types of force traces are obtained depending on the inhomogeneity length and strength. For short inhomogeneities, a "sawtooth" force trace is obtained, with a force jump corresponding to release of the entire inhomogeneity at once, as observed in unzipping of natural-sequence DNA (Bockelmann et al., Biophy. J. 82, 1537 (2002)). For longer inhomogeneities, traces with force plateaus are obtained, corresponding to a gradual unpeeling of the strongly bound region. We find that inhomogeneities are disrupted in sequence giving rise to a succession of force spikes superposed on the baseline unzipping force of 15pN. The height of the force pulses diminishes as regions further down the molecule are unzipped, and asymptotically the force response approaches that of DNA without large stability-enhanced islands. Our model also allows us to describe the transition between intact and disrupted binding zones by thermally activated kinetics. We then analyze the related situation where multiple proteins are bound at specific points on the DNA with or without cooperative interactions between proteins, and where the removal of each protein is required for unzipping to proceed further along the DNA. The proteins bind stably to dsDNA and also to single-stranded DNA (ssDNA) but with a lower binding enthalpy. The force jumps correspond to the extra mechanical work that has to be done to overcome the large protein binding enthalpy to either dsDNA or ssDNA. Each force jump leads to the dissociation of a corresponding protein, but we do not find simultaneous release of cooperatively stabilized proteins under the range of interactions strengths considered here. Our model is then used to describe recent experiments where the stability of nucleosome and nucleosome-analogues was assayed by mechanically unzipping the DNA template. Using physiological values of model parameters we find good agreement with the data.

Theoretical Analysis for Force Transmissibility and Jump Phenomena of Duffing Spring Type Vibration Isolator

In order to study the common nonlinear characteristics of the vibration isolator, a single degree of freedom system with cubic restoring force is introduced to describe the nonlinear vibration isolation system in the paper and the harmonic balance method was applied to investigate the primary resonance near the natural frequency of the system. Based on Routh–Hurwitz stability criterion, it was clarified theoretically that the region surrounded by the curve of the vertical tangential points in the curve cluster of the primary resonance amplitude frequency characteristics was instable. In addition, the equations of the jump frequency and force transmissibility were derived. The calculated results showed that the jump, hysteresis, stable and instable phenomena would take place for the force transmissibility of the isolator system and the effect of high frequency components of the transmitting force was limited; the damping, coefficient of nonlinear restoring force and the excitation amplitude had an influence on the force transmissibility whose frequencies were in the region of the resonance frequencies but not on those whose frequencies were lower than the resonance frequencies, and only the damping would affect the force transmissibility whose frequencies were higher than the resonance frequencies. Finally, the equation of the start frequency, on the condition that the force transmissibility was less than 1, was presented.

Reduction of stilling basin length with tall end sill

Experiments were conducted to characterize forced hydraulic jumps in stilling basins for enforced cases due to tail water level or dam site arrangement and construction. The case with a single tall sill was simulated in a horizontal flume downstream of a sluice gate. Results of experiments are compared with the classical hydraulic jump, and significant effect of tall sill on dissipation of energy in shorter distance was confirmed. Furthermore, the generated jumps were classified based on the ratio of sill height to basin length, and a simple design criterion was proposed to estimate the basin length for a desired jump and particular inflow.

Forced Hydraulic Jumps below Abrupt Expansions

A new approach for studying forced hydraulic jumps below abrupt symmetric and asymmetric expansions is introduced. Intensive experiments for two expansion ratios, one asymmetry ratio, and different solid sill heights and locations were conducted specifically for the spatial jump case as a critical design case. A new parameter correlating sill height and sill location is presented and found to play an important role in the basin’s design, scour, and flow patterns. Using this parameter gives distinguished relationships for predicting sequent depth, energy dissipation, and basin length for the preliminary or operating design in expected or unexpected flow conditions. Empirical equations and experimental regression curves are introduced to predict the sequent depth and the basin length at any sill height and sill location. Scour and flow patterns are intensively studied experimentally and qualitatively described for the symmetric and asymmetric basins with or without sills for the spatial jump. A numerical ex...

Consistent treatment of boundaries with mortar contact formulations using dual Lagrange multipliers

Computational modelling of contact problems raises two basic questions: Which method should be used to enforce the contact conditions and how should this method be discretised? The most popular enforcement methods are the Lagrange multiplier method, the penalty method and combinations of these two. A frequently used discretisation method is the so called node-to-segment approach. However, this approach might lead to problems like jumps in contact forces, loss of convergence or failure to pass the patch test. Thus in the last few years, several segment-to-segment contact algorithms based on the mortar method were proposed. Combination of a mortar discretisation with a penalty based enforcement of the contact conditions leads to unphysical penetrations. On the other hand, a Lagrange multiplier mortar method requires additional unknowns. Hence, condensation of the Lagrange multipliers is desirable to preserve the initial size of the system of equations. This can be achieved by interpolating the Lagrange multipliers with so-called dual shape functions. Discretising two contacting bodies leads to opposed contact surface representations of finite element edges, called slave and master elements, respectively. In current versions of dual Lagrange multiplier mortar formulations an inconsistency at the boundary appears when only a part of a slave element (instead of the entire element) belongs to the contact area. We present a modified definition of the dual shape functions in such slave elements. The basic idea is to construct dual shape functions that fulfill the so-called biorthogonality condition within the contact area. This leads to consistent mortar matrices also in the boundary region. To avoid ill-conditioning of the stiffness matrix, the modified mortar matrices are weighted with appropriate weighting factors. In doing so, the corresponding modified Lagrange multiplier nodal values are of the same order as the unmodified ones. Various examples demonstrate the performance of the modified mortar contact algorithm.