Abstract Focused on the common problem of easy buckling of specimens in the tension-compression axial stress control fatigue test of automobile sheet steel, a stress fatigue test method for thickness less than 2.5 mm was proposed. For two kinds of automobile sheet materials with different strength levels and thicknesses, fatigue specimens with different widths of test section, parallel lengths, and fillet radii are designed. Without the anti-buckling device, high-cycle fatigue tests with a stress ratio Rs = −1 at different stress levels were carried out, and the shape and size of the fatigue specimens of automobile sheet materials were determined by the number of fatigue life cycles of specimens with different sizes under the same stress cycle form. The results show that for automobile sheet steel with a thickness of 1.0–2.5 mm, the recommended sizes of the uniform-gauge fatigue specimens are width of test section w = (1–3) t, parallel length Lp = (1–3) w, and fillet radius r = (3–10) t. For automobile sheet steel with a thickness less than 1 mm, because the time-displacement curve jitters during the test, it is recommended to install an anti-buckling device, specifically the anti-buckling device with mortise and tenon structure by self-design and with a flattop structure recommended by ISO 1099-2017, Metallic Materials—Fatigue Testing—Axial Force-Controlled Method.