Forage Resources Research Articles

Endophyte Infected Tall Fescue: Plant Symbiosis to Animal Toxicosis.

Endophyte-infected fescue is a major cool season forage used for livestock production in the United States and through other areas of the world. A unique aspect of this forage resource is the symbiotic relationship with an endophytic fungus (Epichloë coenophiala) that has detrimental impact on herbivores due to toxic ergot alkaloids. Research over the past 50 years has unveiled details regarding this symbiotic relationship. This review focuses on the origin of tall fescue in the United States and the consequences of its wide-spread utilization as a livestock forage, along with the discovery and toxicodynamics of ergot alkaloids produced by E. coenophiala. The majority of past ergot alkaloid research has focused on observing phenotypic changes that occur in livestock affected by ergot alkaloids, but recent investigation of the metabolome, transcriptome, and proteome have shown that fescue toxicity-related illnesses are much more complex than previous research suggests.

Ecological Intensification of Food Production by Integrating Forages

Forage crops have the potential to serve multiple functions, providing an ecological framework to sustainably intensify food production, i.e., ecological intensification. We review three categories of forages (annual forages, perennial forages, and dual-use perennial crops/forages) we believe hold the greatest promise for ecologically intensifying food production. Annual cover crops can provide additional forage resources while mitigating nutrient losses from agricultural fields when they are intercropped with, interseeded into, or following an annual crop, for instance. The integration of perennial forages either temporally, such as annual crop rotations that include a perennial forage phase, or spatially, such as the intercropping of perennial forages with an annual cash crop, provide weed suppression, soil quality, and yield and crop quality benefits. Dual-use crops/forages can provide forage and a grain crop in a single year while providing multiple ecological and economic benefits. However, tradeoffs in balancing multiple functions and limitations in reducing the risks associated with these practices exist. Advancing our understanding of these systems so we can overcome some of the limitations will play a critical role in increasing food production while promoting positive environmental outcomes.

Transforming livestock productivity through watershed interventions: A case study of Parasai-Sindh watershed in Bundelkhand region of Central India

CONTEXTGlobal experiences reveal the positive impact of watershed-based interventions in improving livelihoods and environmental security. In the drylands, increasing forage resources and improving livestock productivity is a critical challenge. OBJECTIVESThe overarching aim of this paper is to analyse the impact of watershed-based interventions on livestock population, productivity, fodder resources, and biomass availability. The paper describes the interrelationship between land, water, crop, and livestock and how the gap in forage deficit can be bridged through a range of watershed interventions. METHODSThe study was undertaken in the Parasai-Sindh watershed of Central India between 2011 and 2016. A 300-year-old defunct haveli (a traditional rainwater harvesting structure) was renovated and nine check dams along the drainage line were constructed. Nearly 25,000 running-meters of field bunds were constructed. Large fields (2-3 ha) were divided into relatively smaller plots (0.3–0.5 ha) to reduce runoff velocity. The impact of watershed interventions on water availability, livestock productivity, forage yield, and income was studied through hydrological monitoring, primary household survey and principal component analysis (PCA) biplot and hierarchical clustering. RESULTS AND CONCLUSIONSThe watershed interventions enhanced groundwater availability leading to greater fodder availability, 22% increase in livestock population (ACU) and a 120% increase in milk production over a period of five years. The bovine population recorded a surge of 193% (cattle) and 32% (buffalo) over this period. Mean dry matter (DM, fodder) availability also increased from 10 t/household/year to 16.7 t/household/year owing to improved water availability. Groundnut (rainy season) and wheat (post rainy season) contributed significantly as forage resources. There was an annual forage demand of 5560 t DM for a livestock population of 2175 ACU in 2011 and of 6770 t DM for a livestock population of 2650 ACU in 2016. Annual forage biomass availability was estimated at 4219 t DM in 2011 and 6977 t DM in 2016. There was a deficit of 1341 t DM (24%) in 2011 which turned into a 3% surplus of 210 t DM in 2016 through watershed-based interventions. With increased cropping intensity and milk production, average annual household income increased from US$ 1325 to US$ 2430 over the five-year period. SIGNIFICANCEThis case study clearly illustrates the impact of watershed-based interventions on livestock population, productivity, forage resources, and biomass availability to bridge the gap in forage deficit. Its findings serve as a guide to widely scale up watershed-based interventions for improved water and biomass availability, and livestock productivity in semi-arid and dryland regions.

Перспективные сортообразцы козлятника восточного в Северо-Западном регионе РФ

По урожайности зелёной массы сортообразец F6 превысил контроль на 40%, а по сбору сырого протеина — на 45%. По урожайности зелёной массы сортообразец F6 превысил контроль на 40%, а по сбору сырого протеина — на 45%. Bioclimatic potential of the Pskov region provides high-quality seeds of almost all species of gramineous and some species of legumes. The primary role in forage production belongs to sown grasses both in fields (182.8 thousand hectares) and in grasslands (natural hayfields (230 thousand hectares) and pastures (244 thousand hectares). New varieties of perennial grasses cultivated using modern practices are promising means to increase yield and quality of forage resources. Breeding new varieties of eastern goat’s rue was carried out during 2018–2021 at the nurseries of the Federal Research Center of Fibre Crops. The test nursery was established in 2018 using promising genotypes selected earlier in the intercross nursery (F6, F7 и F8) and clone nursery (26-2-8, 27-1-14 and 27-2-15). The variety “Yubilyar” (the Pskov Agricultural Research Institute) performed as the standard. The test nursery selected genotypes having the number of valuable morphological and economically-important traits. Genotypes were evaluated according to their morphology, green mass and seed productivity as well as forage quality. Such genotypes as F6, F7 and F8 stood out due to their height, seed productivity, forage mass yield and quality. Genotype F6 exceeded the standard by 40% in green mass yield and 45% — in crude protein content.

Genetic Transformation of Apomictic Grasses: Progress and Constraints.

The available methods for plant transformation and expansion beyond its limits remain especially critical for crop improvement. For grass species, this is even more critical, mainly due to drawbacks in in vitro regeneration. Despite the existence of many protocols in grasses to achieve genetic transformation through Agrobacterium or biolistic gene delivery, their efficiencies are genotype-dependent and still very low due to the recalcitrance of these species to in vitro regeneration. Many plant transformation facilities for cereals and other important crops may be found around the world in universities and enterprises, but this is not the case for apomictic species, many of which are C4 grasses. Moreover, apomixis (asexual reproduction by seeds) represents an additional constraint for breeding. However, the transformation of an apomictic clone is an attractive strategy, as the transgene is immediately fixed in a highly adapted genetic background, capable of large-scale clonal propagation. With the exception of some species like Brachiaria brizantha which is planted in approximately 100 M ha in Brazil, apomixis is almost non-present in economically important crops. However, as it is sometimes present in their wild relatives, the main goal is to transfer this trait to crops to fix heterosis. Until now this has been a difficult task, mainly because many aspects of apomixis are unknown. Over the last few years, many candidate genes have been identified and attempts have been made to characterize them functionally in Arabidopsis and rice. However, functional analysis in true apomictic species lags far behind, mainly due to the complexity of its genomes, of the trait itself, and the lack of efficient genetic transformation protocols. In this study, we review the current status of the in vitro culture and genetic transformation methods focusing on apomictic grasses, and the prospects for the application of new tools assayed in other related species, with two aims: to pave the way for discovering the molecular pathways involved in apomixis and to develop new capacities for breeding purposes because many of these grasses are important forage or biofuel resources.

Human-modified landscapes provide key foraging areas for a threatened flying mammal: The grey-headed flying-fox.

Urban expansion is a major threat to natural ecosystems but also creates novel opportunities that adaptable species can exploit. The grey-headed flying-fox (Pteropus poliocephalus) is a threatened, highly mobile species of bat that is increasingly found in human-dominated landscapes, leading to many management and conservation challenges. Flying-fox urbanisation is thought to be a result of diminishing natural foraging habitat or increasing urban food resources, or both. However, little is known about landscape utilisation of flying-foxes in human-modified areas, and how this may differ in natural areas. Here we examine positional data from 98 satellite-tracked P. poliocephalus for up to 5 years in urban and non-urban environments, in relation to vegetation data and published indices of foraging habitat quality. Our findings indicate that human-modified foraging landscapes sustain a large proportion of the P. poliocephalus population year-round. When individuals roosted in non-urban and minor-urban areas, they relied primarily on wet and dry sclerophyll forest, forested wetlands, and rainforest for foraging, and preferentially visited foraging habitat designated as high-quality. However, our results highlight the importance of human-modified foraging habitats throughout the species’ range, and particularly for individuals that roosted in major-urban environments. The exact plant species that exist in human-modified habitats are largely undocumented; however, where this information was available, foraging by P. poliocephalus was associated with different dominant plant species depending on whether individuals roosted in ‘urban’ or ‘non-urban’ areas. Overall, our results demonstrate clear differences in urban- and non-urban landscape utilisation by foraging P. poliocephalus. However, further research is needed to understand the exact foraging resources used, particularly in human-modified habitats, and hence what attracts flying-foxes to urban areas. Such information could be used to modify the urban foraging landscape, to assist long-term habitat management programs aimed at minimising human-wildlife conflict and maximising resource availability within and outside of urban environments.

Allocation of foliar-P fractions of Alhagi sparsifolia and its relationship with soil-P fractions and soil properties in a hyperarid desert ecosystem

Desert vegetation in the juncture of the Qira oasis and the Taklamakan Desert typically grows on phosphorus (P)-poor soils. Alhagi sparsifolia Shap. (A. sparsifolia), as widely distributed natural vegetation in this area, not only has crucial ecological effects of wind sheltering and sand fixation, but also is an excellent forage resource. Researchers are increasingly interested in the foliar-P utilization strategy of A. sparsifolia that allows it to survive in extremely P-impoverished soils. Therefore, A. sparsifolia growing in four different soil sites with differing availability of P in this area were studied, and leaves and 0–100 cm soils were collected. Foliar total nitrogen (N), total-P and P fractions (metabolic-P, nucleic acid-P, structural-P, and residual-P) were determined. Soil labile-P (resin-P, NaHCO3-Pi, and NaHCO3-Po), moderately labile-P (NaOH-Po and NaOH-Pi), stable-P (HCl-P and residual-P), and basic soil properties were determined. Our findings showed that A. sparsifolia growth was highly likely to be limited by soil-P in this area, and the concentrations of foliar total-P and young leaf metabolic-P increased with increasing soil-P availability, while foliar-N was relatively constant. Comparatively, A. sparsifolia allocated a higher proportion of foliar-P to nucleic acid-P and a lower proportion to structural-P, as soil-P availability decreased. Moreover, foliar metabolic-P, nucleic acid-P, and structural-P had the strongest positive response to soil labile-P in topsoil. Furthermore, soil electrical conductivity (EC), labile-P, and moderately labile-P influenced foliar-P fractions. Comprehensively, variation in foliar-P allocation patterns of A. sparsifolia varied across the four soils with differing available-P and were closely related to soil-P fractions and properties. These results will help us better understand A. sparsifolia foliar-P utilization strategy and their potential responses to environmental nutrients.

Influence of habitat quality and resource density on breeding‐season female monarch butterfly Danaus plexippus movement and space use in north‐central USA agroecosystem landscapes

Abstract The eastern North American monarch butterfly is at risk of quasi‐extinction due, in part, to the loss of breeding habitat in agricultural landscapes of the USA Midwest. Because adult females are not patch residents, egg abundance and distribution across the landscape are a function of their perceptual range, flight directionality and flight step lengths. Conservation actions that account for habitat use in agricultural landscapes can enhance functional connectivity. Field‐captured females (n = 114) were released in a 64‐ha area containing restored prairies, grass‐dominated fields and crop fields in Floyd County, Iowa, USA, and two 1,000 m linear north–south sections of grass‐dominated roadside along secondary roads (~35 ha) with different proximity to prairie habitat in rural Story County, Iowa. Radio‐tagged or untagged monarchs were released in areas with high density, low density and zero density of forage and oviposition resources, as well as on habitat edges between high‐ and zero‐density habitats. Monarchs were observed for 1 hr. Radio‐tagged individuals that flew beyond visual detection were relocated using handheld radiotelemetry. Monarchs moved within and between habitat classes and typically performed upwind search behaviour. Monarchs successfully located resources, with some flying over 500 m to find high‐density areas, providing evidence that the monarch's perceptual distance is >100 m. Regardless of habitat class or field site, most step lengths were <50 m, and turn angles were directional. Large steps (≥50 m) crossing habitat boundaries occurred with approximately half of the monarchs, which may indicate initiation of long‐range searches for suitable habitat, consistent with their vagile behaviour. Establishing habitat patches 50 m apart in agricultural landscapes would facilitate efficient movement. Synthesis and applications. This study provides an extensive dataset of directly observed breeding‐season monarch butterflies to assess the utilization of agricultural landscapes. Documentation of step lengths >50 m in complex, agricultural landscapes would not have been possible without the aid of radiotelemetry. Results provide improved estimates of perceptual range and flight patterns within and between habitat patches that support models that simulate natural population dynamics to enable conservation planning at a landscape scale.

Feed and forage development in mixed crop–livestock systems of the Ethiopian highlands: Africa RISING project research experience

AbstractAfrica Research in Sustainable Intensification for Next Generation (RISING) project has conducted action research on livestock feed and forages starting in 2012 to bridge gaps on feed availability and quality. The objectives of this paper are to share approaches on how Africa RISING project enabled smallholder farmers to intensify feed and forage resources; present research findings of selected cultivated forages, fodder trees, and postharvest feed and forage utilization innovations; and highlight key lessons evolving from the feed and forage action research and scaling interventions. The project established partnership with CGIAR and local partners, conducted participatory need assessment, prioritized researchable issues, identified and validated different forages and utilization innovations, and created development partnership to facilitate wider scaling. Training activities and multistakeholder platforms were used to enhance cross learning of project partners. The cultivated forages grown under farmers’ fields and management conditions provided high biomass yield of good nutritional quality. For instance, oat (Avena sativa L.)–vetch (Vicia villosa Roth) mixture, lablab [Lablab purpureus (L.) Sweet], vetch–desho grass (Pennisetum pedicellatum Trin.) intercropping, sweet lupin (Lupinus albus L.), alfalfa (Medicago sativa L.), and fodder beet (Beta vulgaris L.) yielded on average 15, 3.4, 5.5, 2.9, 15.3, and 20.2 t dry matter ha−1, respectively. Animal response trials showed an increase in milk yield of 30–50% with supplementation of oat–vetch mixture and fodder beet. Feed wastage was considerably reduced (>30%) due to the use of feed troughs and storage sheds, with high economic incentives to invest in such innovations. Integrating and intensifying feed and forage resources and postharvest innovations at farm level remains a priority to improve livestock productivity and food security.

Characterization of the complete chloroplast genome of Elymus kamoji (Ohwi) S. L. Chen

Elymus kamoji (Ohwi) S. L. Chen is a perennial herb, had high grazing value and were important forage resources, the study of E. kamoji chloroplast genome (cp genome) provides an important basis for the study of chloroplast genetic engineering and system evolution. Its chloroplast genome was 135,075 bp in length, containing a pair of inverted repeated (IR) regions (20,813 bp), separated by a large single copy region (LSC) of 80,681 bp, and a small single copy (SSC) region of 12,768 bp. Moreover, a total of 129 functional genes were annotated, including 83 mRNA, 38 tRNA genes, and 8 rRNA genes. The phylogenetic relationships of 15 species indicated that E. kamoji was closely related to Elymus sibiricus. This study might contribute to provide a theoretical basis for species identification and biological research.

82 Application of Precision Sensor Technologies, Real-time Data Analytics, and Dynamic Models on Extensive Western Rangeland Grazing Systems

Abstract Approximately 40% of the land use within the Northern Great Plains is dedicated to livestock production, with much of the 89.9 million head of cattle and calves in the U.S. concentrated in this area. Precision livestock management has ushered in a new era of sensors and technology to monitor individual animal’s health, reproductive, and nutritional status in real-time to improve efficiency. Despite these advances, most of the research has been conducted on dairy operations or within feedlot settings. Incorporation on extensive rangeland production systems remains relatively absent (Brennan et al., 2021). This is primarily due to difficulties in studying animals on rangelands caused by heterogeneity of forage resources, variable environmental conditions, and challenges associated with accessing information across vast distances, often without cellular or internet connection. Advances in communication technology are increasingly connecting remote areas, creating new opportunities to improve livestock production efficiency on extensive rangelands using precision technology. Numerous challenges still exist, including applying and integrating multiple technologies across platforms, effectiveness in a real-world setting, technical skills, and knowledge to utilize real-time data, and achieving economic return for livestock producers. Specifically, we discuss the application of precision technologies and mathematical models for improving ruminant nutrition in rangeland systems (Menendez and Tedeschi, 2020). Opportunities exist to refine or develop the next generation of equations/models that more adequately represent nutrient dynamics such as diet selection, supplementation, movement, behavior, water intake, feed conversion efficiency, heat/cold stress, and gain on an individual animal basis. However, effective adoption and adaptability of new technologies/data analytics merit the consideration of potential intended-and-unintended consequences, such as producer dependency on complex hardware and software systems. Hence, precision capabilities coupled with mathematical models are likely the next step to substantially enhance livestock performance in extensive systems when coupled with feasible and reliable long-term strategies.

Dual-Purpose Water Buffalo Production Systems in Tropical Latin America: Bases for a Sustainable Model.

Simple SummaryBuffaloes in the Latin American tropics have gained relevance in recent years thanks to the rusticity and productivity of this species and the potential of these ecosystems. Through a specialized literature review, the options were explored to promote efficient, profitable and sustainable dual-purpose production systems, which implied taking into consideration the characteristics of a fragile environment such as the tropics but with abundant forage resources. Buffaloes have distinctive characteristics with respect to cattle, such as a high fat milk content, ability to produce healthy and heavy calves from dairy cows and their need for thermoregulation, which implies the availability of water ponds and shade in grazing areas. Therefore, we propose the basis for designing intensive rotational grazing models and, under certain silvopastoral conditions, taking advantage of the gregarious behavior of buffaloes. These models require integral forms of management, including the organization and participation of producers in commercial channels, in order to value meat, milk and dairy products, given that their nutritional qualities allow them to be sold as differentiated products. There have been successful experiences in the region with this dual-purpose model, which, with the necessary adaptations, can be strengthened with buffaloes.Tropical regions of Latin America have been incorporated into development in recent decades, with extensive cattle ranching as one of the main economic activities but without adequate planning, drastically degrading the ecosystem. In recent years, buffalo production has been incorporated into the region, with possibilities for development in profitable and sustainable models. To study this option in depth, a broad bibliographic review was carried out focusing on the ecological characteristics of tropical zones and the physiological and productive characteristics of buffaloes. We also investigated the structure and functioning of dual-purpose systems that have worked in cattle and that can be optimized with this alternative animal species. The possibility of taking sustainable advantage of abundant forage resources in the region was detected through intensive grazing models, as well as agrosilvopastoral systems, due to the gregarious qualities of buffaloes and responding to their thermoregulation needs. In this way, the productive and regenerative capacities of the dual-purpose system could be increased, as well as the quality of meat and milk, which could be marketed as differentiated products, taking advantage of their outstanding nutritional qualities. Integral management of the dual-purpose system is proposed, retaking the bases of the original model of family characters, diversified and with low investments and risks, which with specific innovations can be an effective development option for producers in the region.

Dancing bees evaluate central urban forage resources as superior to agricultural land

Abstract Recent evidence suggests that flower‐rich areas within cities could play an important role in pollinator conservation, but direct comparison of floral resources within agricultural and urban areas has proved challenging to perform over large scales. Here we use the waggle dances of honeybees Apis mellifera L. to perform large‐scale landscape surveys at heavily urban or agricultural sites for a key pollinator of wild and crop plants. We analysed 2,827 dances that were performed by 20 colonies in SE England. We show that hive median foraging trip distance is consistently lower at urban sites across the entire season. The sucrose content of collected nectar did not significantly differ between urban and agricultural land, ruling out the possibility that longer foraging distances in agricultural sites were driven by distant but nectar‐rich resources. Within cities, bees preferentially targeted residential areas on foraging trips, while trips to mass‐flowering crops overwhelmingly dominated at agricultural sites. For both land‐use types, distances flown increased in the summer, but there was high variation in temporal patterns between individual sites. Policy implications. From the self‐reported perspective of a generalist pollinator, forage was easier to find in heavily urbanized areas than in the modern agricultural landscapes that we studied. A focus on continuous spatial and temporal provision within agricultural environments is key to redressing this imbalance.

Impacts of land cover change on the plant resources of an endangered pollinator.

One of the key drivers of pollinator declines is land cover change. We documented for the first time the impacts of over three decades of land cover change in Mexico on the plant resources of an endangered migratory pollinator, the Mexican long-nosed bat, Leptonycteris nivalis. This species is considered endangered under national and international criteria due to population declines over 50% in the past 10 years. Pregnant females of this bat species migrate every year following the blooms of Agave spp. from central Mexico to the southern United States; moving pollen over its 1,200 km long migratory corridor and pollinating distant populations of Agave spp. Increases in human populations density and agricultural expansion may be reducing agave habitat over time. The objective of our study is to understand the land cover change trends in the northern range of the bat and identify potential fragmentation patterns in the region. We analyzed changes that occurred in three vegetation types where agaves are found in five time periods 1985, 1993, 2002, 2007 and 2011. The area of the three vegetation types selected was reduced by using only the overlap with potential agave habitat created with ecological niche modeling algorithms to obtain the available agave habitat. We then calculated fragmentation metrics for each period. We found a significant portion of habitat lost mainly due to expansion in agriculture. The total number of patches increased after 1985. Only 9% of the available agave habitat in 2011 is inside the limits of protected areas. We recommend restoring agave populations in depleted areas to help prevent soil erosion and provide multiple socio-economic benefits for the region in the short term, and, in the long-term maintaining foraging resources for nectar-feeding bats.

Resilience strategies of West African pastoralists in response to scarce forage resources

Finding sufficient natural fodder resources to feed livestock has become a challenge for herders in the Sahel zone of Burkina Faso. Despite the existence of pastoral reserves, the issue of fodder shortage remains unsolved. This article highlights the changes in behaviour and the evolution of pastoral practices caused by the scarcity of forage resources. These changes are defined and classified as resilience strategies. Thus, this paper aims to analyse these strategies using new semantics that calls for other forms of perceptions or approach to the questions of pastoralists’ resilience strategies. Interviews (semi-structured and casual conversations), ethnographic observations and ethnobotanical surveys were used to collect data. In rangelands, such high value fodder species as Andropogon gayanus, Pennisetum pedicellatum and Dactyloctenium aegyptium that were abundant herbaceous plants during the last decades are disappearing. Concomitantly, species with lower forage value, such as Senna obtusifolia, which are more resilient to ecological disturbance factors, are colonizing rangelands. Faced with these ecological changes, pastoralists are trying to redefine and reconfigure their practices, and this implies a redefinition of their identity. They use resilience strategies such as mowing grasses, building up fodder bundles, conserving crop residues, exploiting Senna obtusifolia (a previously neglected species), using woody fodder and adapting the type of livestock and the size of the herds to the ability of pastoralists to feed them. Strategies that are older than these are the integration of agriculture with livestock and decollectivized transhumance. It is these resilience strategies that this article exposes and analyses as defence mechanisms of Sahelian pastoralists in the face of the depletion of forage resources in their environments.

Transcriptome and metabolome analysis reveals anthocyanin biosynthesis pathway associated with ramie (Boehmeria nivea (L.) Gaud.) leaf color formation

BackgroundThe bast fiber crop ramie can be used as high-quality forage resources, especially in tropical or subtropical region where there is lack of high-quality protein feed. Hongxuan No.1 (HX_1) is a unique ramie variety with a light reddish brown leaf color, which is obviously different from elite cultivar, Zhongzhu No.1 (ZZ_1, green leaf). While, the regulatory mechanism of color difference or secondary metaboliates synthesis between these two varieties have not been studied.ResultsIn this study, phenotypic, transcriptomic and metabolomic analysis of HX_1 and ZZ_1 were conducted to elucidate the mechanism of leaf color formation. Chromaticity value and pigment content measuring showed that anthocyanin was the main metabolites imparting the different leaf color phenotype between the two varieties. Based on LC/MS, at least 14 anthocyanins were identified in leaves of HX_1 and ZZ_1, and the HX_1 showed the higher relative content of malvidin-, pelargonidin-,and cyanidin-based anthocyanins. Transcriptome and metabolome co-analysis revealed that the up-regulated expression of flavonoids synthesis gene was positively correlated with total anthocyanins accumulation in ramie leaf, and the differentfially expression of “blue gene” (F3’5’H) and the “red gene” (F3’H) in leaves bring out HX_1 metabolic flow more input into the cyanidin branch. Furthermore, the enrichment of glycosylated modification pathway (UGT and AT) and the expression of flavonoid 3-O-glucosyl transferase (UFGT), anthocyanidin reductase (ANR), in leaves were significantly influenced the diversity of anthocyanins between HX_1 and ZZ_1.ConclusionsPhenotypic, transcriptomic and metabolomic analysis of HX_1 and ZZ_1 indicated that the expression levels of genes related to anthocyanin metabolism contribute to the color formation of ramie variety. Anthocyanins are important plant secandary metabilates with many physiological functions, the results of this study will deepened our understanding of ramie leaf color formation, and provided basis for molecular breeding of functional forage ramie.

Riparian Ecological Infrastructures: Potential for Biodiversity-Related Ecosystem Services in Mediterranean Human-Dominated Landscapes

Riparian Ecological Infrastructures are networks of natural and semi-natural riparian areas located in human-dominated landscapes, crucial in supporting processes that directly or indirectly benefit humans or enhance social welfare. In this study, we developed a novel multimetric index, termed Habitat Ecological Infrastructure’s Diversity Index (HEIDI), to quantify the potential of Riparian Ecological Infrastructures in supporting biodiversity, and related ecosystem services, in three managed landscapes: Intensive Agriculture, Extensive Agriculture, and Forest Production. Metrics describing the structure, composition, and management of riparian vegetation and associated habitats were used to derive the potential of Riparian Ecological Infrastructures in supporting three distinct biological dispersal groups: short-range dispersers (ants), medium-range dispersers (pollinators), and long-range dispersers (birds, bats, and non-flying small mammals). The composition of floristic resources, assessed by identifying trees and shrubs at the species and genus level, and herbaceous plants at the family level, was used as a proxy to evaluate the potential of Riparian Ecological Infrastructures in promoting seed dispersal and pollination ecosystem services provided by the three biological communities. Our research evidenced that Riparian Ecological Infrastructures located in the Forest Production and Intensive Agriculture landscapes exhibited the highest and lowest potential for biodiversity-related ecosystem services, respectively. The Forest Production landscape revealed higher suitability of forage resources for short- and medium-range dispersers and a higher landscape coverage by Riparian Ecological Infrastructures, resulting in more potential to create ecological corridors and to provide ecosystem services. The Riparian Ecological Infrastructures located in the Extensive Agriculture landscape seemed to be particularly relevant for supporting long-ranges dispersers, despite providing less habitat for the biological communities. Land-use systems in the proximity of Riparian Ecological Infrastructures should be sustainably managed to promote riparian vegetation composition and structural quality, as well as the riparian width, safeguarding biodiversity, and the sustainable provision of biodiversity-related ecosystem services.

COMPOSICIÓN BOTÁNICA Y EVALUACIÓN NUTRICIONAL IN VITRO DE PASTO KIKUYO COMPARADO CON GRAMÍNEAS DE CLIMA TEMPL

<p><strong>Background: </strong>Intensive continuous grazing of temperate grass and legume pastures has been a feeding strategy adopted in small-scale dairy systems (SSDS) in the highlands of central Mexico that reduces costs and increases profitability of farms. However, temperate grasses have low persistency due to agroclimatic and management conditions, so that pastures are invaded by kikuyu grass (<em>Cenchrus clandestinus</em>), a sub-tropical grass that may be a valuable forage resource in these systems. There are not many studies in Mexico on kikuyu grass pastures and on the relationship between botanical composition, nutritional value and ruminal fermentation parameters, important aspects for their inclusion in feeding strategies for dairy cattle. <strong>Objective: </strong> The objective was to determine the botanical composition and to assess the <em>in vitro </em>digestibility of dry matter (DM), organic matter (OM), and neutral detergent fibre (NDF), and to characterize the kinetics of <em>in vitro </em>ruminal fermentation of a pasture based on with kikuyu grass (KY) in comparison to pastures based on festulolium cv. Spring Green (FL), tall fescue cv. TF-33 (TF), and perennial ryegrass cv. Pay Day (RG), all associated with white clover. <strong>Methodology: </strong> There were four sampling periods every 14 days. On the last day of each period, five random samples in each pasture were cut to ground level to determine botanical composition by manual separation, and 200g samples from each pasture taken by simulated grazing to determine digestibilities and the <em>in vitro </em>fermentation dynamics. Statistical analyses were following a split-plot model. <strong>Results: </strong>There was a higher proportion of grasses in KY and a better <em>in vitro </em>nutritional value for RG, related to a higher content of structural carbohydrates in KY, being a subptropical grass compared to temperate pastures with lower content of cell walls. <strong>Results: </strong>There was a higher proportion (P<0.05) of live grass in KY. There were no differences (P>0.05) among pastures for <em>in vitro </em>nutritional value. There were differences (P<0.05) among pastures and periods for the insoluble fraction fermentation rate (C<sub>B</sub>) and for the interaction between pastures and periods for gas production in 4.0 h of the soluble fraction (A) and the soluble carbohydrate fermentation rate (C<sub>A</sub>), but no differences between pastures, periods or the interaction (P>0.05) for other parameters of ruminal fermentation (lag time and total gas production). <strong>Implications: </strong> Pastures of kikuyu grass associated with white clover have a similar <em>in vitro </em>nutritional value as pastures of temperate grasses representing a valuable forage resource for SSDS. <strong>Conclusion: </strong>In spite of differences in some <em>in vitro </em>ruminal fermentation parameters, the <em>in vitro </em>nutritional value of kikuyu grass pastures associated with white clover is similar to pastures based on temperate grasses with white clover.</p>

Functional heterogeneity of habitats and dry season forage variability in an Okavango Delta landscape, northern Botswana

The late dry season is a period when forage resources become limited in some African savannas that can induce a strong demographic bottleneck in herbivore populations with limited access to resource heterogeneity. This study determined forage quality and quantity between wetlands and drylands habitats during the late dry season. We subjectively sampled forage characteristics in four habitat types around the distal reaches of the Tsum Tsum floodplains of the Okavango Delta during the late dry season of 2015. The habitat types were mopane and sandveld woodland far from floodplains (two major dryland habitat types), sandveld habitats that receive soil moisture inputs from adjacent floodplains (wet sandveld) and sedgeland habitat within the floodplain. Leaves collected from forage in each habitat type were oven dried, milled and analysed for total nitrogen. Data of greenness, height, biomass and protein were analysed using Kruskal-Wallis test. Forage in wet sandveld had by far the highest protein content (~16%) of all habitat types but forage height and biomass were very low, whereas sedgelands had the highest biomass of adequate-quality forage, while dryland communities had the lowest quantity and quality of forage. Thus, foraging between a higher-biomass, adequate-quality reserve resource (sedgeland) and a high-quality but low-quantity bridging resource (wet sandveld) can help to ensure a balanced protein and fibre intake during the late dry season. Our study demonstrated the importance of wetland habitats for providing green forage with sufficient biomass and protein to sustain herbivores over the late dry season.

Alter fire timing to recouple forage nutrients with herbivore nutrient demands

Forests typically have limited high quality nutrition available to meet the seasonal needs of white-tailed deer (Odocoileus virginianus) during summer. Prescribed fire is used to enhance deer habitat in pyric-disclimax communities but is normally applied during the dormant season. Historically, lightning-ignited fires in pine systems occurred during summer in the southeastern US, but fire ignited by humans occurred at all times of the year and was most common in fall/winter. The different timing of prescribed fire may be important when trying to couple forage resources with nutritionally demanding periods for herbivores. We evaluated fire-timing effects (dormant season, March; growing season, June; and unburned control) on nutrient availability (crude protein, % phosphorus, and biomass) and nutritional carrying capacity (NCC) for moderate-high use forages of white-tailed deer during summer of 2018 and 2019 in nine mid-rotation loblolly pine stands. Fire increased nutrient content the year of the burn and the increase persisted into the second growing season following growing season fire. Dormant season fire increased protein- and phosphorus- based NCC for two growing seasons but growing season fire reduced biomass during the first year and delayed NCC improvement until the second year. Prescribed fire can be timed to manipulate forage quality, quantity, and improve NCC to recouple forage nutrient supply with herbivore seasonal nutrient demands. We recommend land managers consider diversifying fire timing across the landscape in pyric disclimax systems to support the changing nutrient requirements of large herbivores.