Objective To measure the double-bundle footprints of the anterior cruciate ligament (ACL) three-dimensionally reconstructed by dual-source three-dimensional computed tomography (DSCT) so as to provide parameters for anatomic double-bundle ACL reconstruction in clinic.Methods This study enrolled 55 healthy volunteers,32 men and 23 women,20 to 50 years of age (average,28 years).One hundred and ten knees were scanned and processed with 64-slice spiral CT workstation (GE,Volume Share2-AW 4.4 version).The lateral wall of the intercondylar notch and the ACL femoral footprint were reconstructed three-dimensionally.The angle,length and width of the footprint,the distance between the double-bundle centers (DDBC) (anteromedial bundle and posterolateral bundle),and the distance from the footprint edge to the margin of the articular cartilage were measured.Results In this serial,the footprint angle averaged 6.8° ± 4.6°,the long footprint axis 16.8 ± 1.7 mm,the short footprint axis 7.5 ± 1.4 mm,DPCM 1.9 ± 1.0 mm,DDCM 1.5 ± 1.3 mm,and DDBC 8.0 ± 1.0 mm.There were no significant differences concerning footprint angle,long footprint axis,short footprint axis,DPCM,DDCM and DDBC between the left and the right knees in men and in women ( P > 0.05) except concerning DPCM between the left and the right knees in women ( P < 0.05).There were no significant differences concerning footprint angle and short footprint axis between men and women (P> 0.05),but there were significant differences concerning long footprint axis,DPCM,DDCM and DDBC between men and women ( P < 0.05).Conclusions Measurements of femoral ACL footprints by DSCT reconstruction in normal population are reliable and representative.The footprint angle,DDBC and the distance from the footprint edge to the margin of the articular cartilage vary from individual to individual. Key words: Anterior cruciate ligament; Femur; Tomography; spiral computed