Footpad Dermatitis Scores Research Articles

An automatic method for scoring poultry footpad dermatitis with deep learning and thermal imaging

Footpad dermatitis (FPD) is a common poultry condition that can negatively influence chickens’ production, welfare, and health. However, no automated tool for monitoring FPD in live chickens is currently available. The objective of this study was to develop and optimize deep learning models to monitor hens’ FPD scores (i.e., 0–2 scale with higher scores indicating poorer footpad conditions). A total of 700 Hy-Line W-36 hens were raised in four cage-free housing systems integrated with Electrostatic Particle Ionization and various bedding materials. A GoPro camera with an upward lens was placed inside a transparent box. Individual laying hens were placed on the top surface of the box to acquire RGB images. In addition, a thermal camera was used to record RGB and thermal images of footpads, and the images were manually scored to assess their footpad conditions. Preprocessing techniques (e.g., filtration, separation, and augmentation) were deployed to enhance dataset quality and size. Moreover, YOLOv8 models (YOLOv8n, YOLOv8s, YOLOv8m, YOLOv8l, and YOLOv8x) and YOLOv7 models (YOLOv7 and YOLOv7x) were comparatively evaluated for predicting FPD scores. The results show that the YOLOv8l outperformed other models, with higher recall (96.6 %), mAP@0.50 (97.0 %), and F1-score (95.0 %). Additionally, the YOLOv8l-FPD model exhibited a high mAP@0.50 for score 0 (98.0 %), score 1 (95.0 %), and score 2 (97.9 %) and F1-score (95.0 %) for all FPD scores. Notably, using thermal images could result in faster convergence of model training and slightly better FPD score prediction performance than RGB images. The proposed technique can be useful for non-invasive automatic FPD scoring and further improve automation levels and animal welfare in the egg industry.

PSXI-6 Effects of termite frass inclusion in free range broiler beddings on growth, footpad dermatitis score, and blood profiles

Abstract Research on insects as feed has gained attention in the last decade. Among insect species, we have focused on termites, which degrade lignocellulose and synthesize protein by employing gut microbiota. In particular, the damp-wood termite Hodotermopsis sjostedti subsisting on rotten wood, excretes a substantial portion of wood as frass, serving as their primary nest material. Termite frass, a by-product of termite production, has been found to comprise approximately 83% lignin, suggesting potential antibacterial properties. In this study, the effects of termite frass inclusion in free-range broiler chicken bedding on growth, meat and organ weights, blood profile, and footpad dermatitis (FPD) score were examined. Broiler chicks [n = 30; 0 d of age and initial body weight (BW) = 47.1 ± 0.51 g] were divided into three groups and resided in a 1.3 m2 pen fed on a commercial diet. The groups were subjected to one of the three bedding treatments: 7.5 kg sawdust (0% frass), 7.5 kg sawdust plus 7.5 kg termite frass (100% frass), and 7.5 kg sawdust plus 15.0 kg termite frass (200% frass). Intakes and BW were measured weekly, and the FPD score was assessed at 3, 5, 6, and 7 wk. At 50 d of age, the chickens were slaughtered for meat and organ weights and blood profile analyses. Growth data, meat and organ weights, and blood profiles were analyzed by GLM of SAS, and the weekly FPD score was analyzed by GLIMMIX as binomial data. Average daily gain and final BW were greater (P < 0.001 and P = 0.003, respectively) in chickens for 0% and 200% frass treatments than in 100%. Thigh and fillet meat weights were similar among the treatments, whereas breasts tended to be lighter (P = 0.064) for 100%. Footpad dermatitis scores tended to be greater (P = 0.085) for 200% than for 100%. Among organ weights tested (e.g., liver, heart, spleen, ventriculus, proventriculus, pancreas, cecum, intestine), heart weight was heavier (P = 0.044), whereas proventriculus was lighter (P = 0.022) for 200% than for 100%. On the other hand, cecum weight tended to be heavier (P = 0.075), and intestine tended to be lighter (P = 0.093) for 0% than for 100% and 200% treatments. Plasma alanine transaminase (ALT) and lactate dehydrogenase (LD) were less (P < 0.001 and P = 0.04, respectively) for 100% and 200% than for 0%. In conclusion, termite frass inclusion in free-range chicken bedding had slightly adverse trends on FPD; however, 200% inclusion did not alter the growth performances of chickens. It also suggested that frass inclusion would have preferable effects on chicken health based on plasma ALT and LD values. Further investigation is required for the different organ weights and their effects, such as heart, proventriculus, cecum, and intestine.

Enhancing Broiler Welfare and Foot Pad Quality through the Use of Medicinal Plant-Based Pellets as Bedding Material

The aim of this study was to investigate the impact of different bedding materials on both production performance and foot quality, assessed through the incidence, severity, and microbiological quality of foot pad dermatitis (FPD). A total of 2400 Ross 308 chickens were raised over 42 days, distributed across eight bedding material types (eight groups × four treatments × 75 chickens each). These pellets either lacked medicinal plant components or included 30% of plant parts, such as oregano (Origanum vulgare), summer savory (Satureja hortensis), lemon balm (Melissa officinalis), salvia (Salvia officinalis), and thyme (Thymus vulgaris). Production performance controls included body weight (BW) at 42 days of age, feed conversion ratio (FCR), and mortality. The severity of FPD was evaluated at 28, 35, and 42 days using a scale ranging from S0 to S3. A microbiological analysis of the feet was based on the FPD score. Broilers raised on straw chaff (bedding: 24% oregano and 6% summer savory, 18% oregano and 12% summer savory, and 24% oregano and 6% thyme) exhibited the highest BW. Straw chaff-reared broilers demonstrated the lowest FCR. The highest mortality occurred on 18% oregano and 12% summer savory bedding, which may have been caused by the high share of summer savory, which could be toxic. At 35 and 42 days, broilers in the 24% oregano and 6% summer savory group exhibited the best foot quality, while those on straw chaff and 18% oregano and 12% summer savory had the worst one. The influence of medicinal plants in the pellets was particularly notable in reducing Listeria sp. This reduction was likely due to the presence of carvacrol from summer savory, quercetin from lemon balm, and thymol from thyme. Based on our research, the best bedding materials for foot quality are 24% oregano with 6% summer savory and 18% summer savory with 12% salvia, while 18% summer savory with 12% lemon balm and 18% summer savory with 12% thymus were the most effective in limiting Listeria sp.

Dietary α-galactosidase and xylanase to improve the nutritional value of corn-soybean-rapeseed meal diets in broiler chickens

ABSTRACT Two experiments were performed to assess the effects of an enzyme preparation (containing α-galactosidase and xylanase) on chicken performance, carcass traits, footpad dermatitis score, and nutrient digestibility. During first experiment, chickens were fed four diets: (1) control diet (PC): corn and soybean-rapeseed meal; (2) PC supplemented with 350 ppm of the enzyme; (3) Negative control (NC): PC with a 4% reduction of apparent metabolizable energy (AME) and crude protein content; (4) NC supplemented with 500 ppm of the enzyme. In the second experiment, chickens were fed PC or PC + enzyme 350 ppm and a digestibility balance was performed. The enzyme supplementation at 350 and 500 ppm significantly improved the overall feed conversion ratio in PC and NC diet, respectively (P < 0.01). Regarding footpad dermatitis score, animals fed NC + enzyme 500 ppm tended to present a lower value than animals fed NC (P < 0.1). Concerning the digestibility balance, PC + enzyme 350 ppm diet presented a higher feed AME and AMEn value than PC diet (P < 0.05). The enzyme also improved the apparent ileal digestibility (AID) of proline (P < 0.05) and tended to increase AID of cysteine (P < 0.1). In conclusion, the enzyme preparation improves birds feed efficiency by increasing energy utilization.

Evaluating the impact of gypsum as a novel bedding material on broiler performance, foot pad health, and fear response

Flue Gas Desulfurization (FGD) gypsum is a byproduct of the coal-fired power plant process commonly used to remove sulfur dioxide emissions from the flue gas. FGD gypsum has numerous industrial, agricultural, and environmental applications. This study aimed to explore a novel approach involving the use of FGD gypsum combined with different litter treatments as bedding for broiler production. It focused on performance metrics, including adjusted feed conversion ratio (AFCR) and average body weight (BW), foot pad dermatitis (FPD), and fear response over 5 consecutive flocks. A total of 1,800 one-day-old Ross 708 chicks were randomly assigned to 24 pens (75 birds/pen), divided into 6 treatment groups (4 pens/treatment), with 5 replications and raised until 42 d old (d). Treatments were gypsum that was decaked (D), rotovated (E), and rotovated then windrowed (F) between flocks. Control treatments using pine shavings were decaked (A), rotovated (B), and windrowed postrotovating (C). AFCR, average BW, and mortality were used as a measure of production. Foot pad dermatitis scores were taken on d42 using a scale of 0 (absence), 1 (mild), and 2 (severe). Response to observer and human approach test were used to measure fear response. Data were analyzed as a 2-way ANOVA (Proc Glimmix) for the main effects of bedding type and litter treatment. Means were identified using Tukey's HSD. No effect of bedding type or litter treatment was found for AFCR, BW, or mortality. FPD scores 2 and 1, were higher with pine shavings than gypsum (P = 0.01 and P = 0.01, respectively). While FPD scores 0 were higher for gypsum than the pine shaving (P = 0.01). No difference in fear response was found among birds raised on any of the gypsum litter treatments and any of the pine shaving litter treatments. Overall, the use of gypsum as bedding results in equivalent production and fear response to pine shavings, while increasing FPD quality when compared to pine shaving.

A walk through the broiler breeder life: how do footpad dermatitis and gait scores develop from rearing to slaughter?

ABSTRACT The two most common animal-based indicators used to evaluate leg health in broiler chickens are footpad dermatitis (FPD) and gait scoring, but these indicators are less explored in broiler breeders. This study is the first to investigate FPD and gait scoring in broiler breeders during their lifespan from rearing to end of life. In total, eight flocks were monitored (four Ross and four Hubbard) at five different timepoints, in rearing (5 and 15 weeks of age), during the production period (25 and 45 weeks of age) and at the end of the production period (approximately 60 weeks of age). At each visit, 50 hens and 25 roosters were gait scored (six-point scale) and footpads from another 50 hens and 25 roosters were evaluated (five-point scale) (total n = 3000 breeders, 2000 hens and 1000 roosters). Litter quality and air quality were measured at each visit. The results showed that the overall prevalence of FPD in rearing was low and that it increased towards the end of the production, with a mean FPD score of 2 out of a maximum 4 in the hens, indicating moderate lesions and 1.5 in the roosters. In all houses, the litter was dry and loose. FPD was not related to the litter quality, but to air quality, especially the ammonia concentration (P < 0.001). Overall, the gait score were good, and increased with age in both hens (P < 0.001) and roosters of both hybrids (P < 0.001).

Efficacy of oat hulls varying in particle size in mitigating performance deterioration in broilers fed low-density crude protein diets

Dietary fibres with increased particle size may develop foregut more efficiently in meat poultry fed diets moderately low in crude protein. The study investigated the performance of broilers fed low-density crude protein diets with the inclusion of oat hulls increasing in particle size. Ross 308 male broiler chicks (n=336) were divided into 48 pens, 12 pens per treatment (7 chicks per pen). Pens were allocated to 4 dietary treatments in mash form; positive control (PC), a standard crude protein diet, negative control (NC), around 5% lower in protein with 5% celite as an inert material, OH400: 5% lower protein diet with 5% oat hulls of geometric mean diameter (GMD) 400 µm, OH850: 5% lower protein diet with 5% oat hulls of GMD 850µm. Birds were fed ad libitum from d 1 to 35 in 3 phases; starter: d 0 to 10, grower: d 10 to 24 and finisher: d 24 to 35. Growth performance was calculated at the end of the trial. Two birds per pen were sampled on d 24 and 35 to collect data on proventriculus and gizzard weights and pooled ileal digesta. Apparent ileal digestibility of amino acids on d 24, and metabolizable energy on d 24 and 35 were recorded for each pen. Litter was sampled from each pen on d 34 to analyze litter N and moisture content. Footpad dermatitis scores of all birds per pen were recorded on d 35. Overall, no difference (P > 0.05) in body weight gain and feed intake was found between the treatments. However, NC and OH400 showed poorer FCR than PC, whereas FCR on PC and OH850 was similar (P > 0.05). Gizzard absolute weight and relative to body weight, and gizzard to proventriculus ratio were higher (P < 0.05) on OH850 compared to PC and NC on d 24 and 35. Gizzard digesta particle size was reduced (P < 0.05) on OH850 compared to all other diets on d 24 and 35. Amino acids digestibility coefficients for aspartic acid and valine increased (P < 0.05) in birds fed OH850 compared to PC, whereas coefficients for several other amino acids were improved compared to NC and OH400. The apparent ileal digestibility of metabolizable energy was similar (P > 0.05) between PC and OH850. Litter moisture and nitrogen, and footpad dermatitis scores were reduced (P < 0.05) on OH850 compared to PC. In conclusion, the inclusion of coarse oat hulls of GMD 850 µm in low-density crude protein diets can be beneficial for the broilers in developing the foregut, utilizing the nutrients efficiently and reducing litter nitrogen and moisture, and footpad scores.

The Effect of Rearing System on Plumage Quality and Foot-Pad Dermatitis in Guinea Fowls and Pheasants

This study aims to determine welfare parameters such as foot-pad dermatitis (FPD) and plumage quality (PQ) scores at different slaughter ages in barn and free-range rearing systems for guinea fowl and pheasants. The birds randomly distributed with half of 200 Pheasant and 200 guinea fowl chicks were reared in both indoor and free-range systems. Game birds were examined for both FPD score and feather score (PQ) at 6, 12, 14, 16 and 18 weeks of age. The litter moisture content was also measured at 14, 16 and 18 weeks of the growing period. In guinea fowls, litter moisture content differed significantly according to the rearing system (P0.05). In terms of FPD, there was no difference in guinea fowl according to the rearing system, gender and slaughter age, but there was a difference in pheasants according to the slaughter age. In guinea fowl, the head PART feather quality was lower than the barn system (P

Effect of silvopasture system on fearfulness and leg health in fast-growing broiler chickens.

A silvopasture system intentionally integrates trees, forages, and livestock, allowing dual land use. These systems can provide high-quality habitat for broiler chickens; however, such systems have not been widely adopted by the broiler industry in the United States. The objective of this study was to examine the effect of silvopasture versus open pasture access on fearfulness and leg health in fast-growing broiler chickens. A total of 886 mixed-sex Ross 708 chicks in Experiment 1 (Exp 1) and 648 chicks in Experiment 2 (Exp 2) were housed in coops and had access to 16 (Exp 1) or 12 (Exp 2) 125m2 silvopasture plots (x̄ = 32% canopy cover) or open pasture plots (no canopy cover) from day 24 of age. Fearfulness was measured using a tonic immobility test (tonic immobility duration), and leg health was assessed by quantifying footpad dermatitis, hock burns, gait, and performing a latency-to-lie test on days 37-39 of age. Birds in the silvopasture treatment were less fearful than birds in the open pasture treatment. Overall, birds in both silvopasture and open pasture systems had excellent leg health. Silvopasture birds had lower footpad dermatitis scores than open pasture birds. Silvopasture birds tended to have worse gait than open pasture birds in Exp 1, but not in Exp 2. Hock burn scores and latency-to-lie did not differ between treatments in Exp 1 or Exp 2. Raising birds in silvopasture reduced fear and improved footpad health compared to birds raised in open pastures, which indicates that silvopasture systems provide some benefits for affective state and leg health in fast-growing broilers.

Effects of supplemental different clay minerals in broiler chickens under cyclic heat stress.

The objective of this study was to investigate the effect of supplementing clay minerals and organic chromium in feed on broiler chicken under heat stress (HS). A total of 90 one-day-old broiler chicken (Arbor Acres) with an initial body weight of 45.0 ± 0.2 g were assigned to five treatment groups (six replications, three birds each cage): 1) NC group, basal diet under room temperature environment; 2) PC group, basal diet under high temperature (HT) environment; 3) ILT group, basal diet + 1% illite + HT; 4) ZLT group, basal diet + 1% zeolite + HT; 5) OC group, basal diet + 400 ppb/kg organic chromium + HT. The ILT and ZLT groups had significantly higher body weight than the PC group in 4 weeks. Apparent total tract digestibility of gross energy was increased in the ILT, ZLT, and OC groups compared to the PC group. The NC group had lower foot-pad dermatitis score than other groups. Escherichia coli population in the cecum and feces was decreased in the ZLT group than in the PC group. Lactobacillus in cecum and feces was significantly increased in the ZLT group than in the PC group. Regarding blood profiles, blood cortisol was decreased in the NC and ILT groups compared to the PC group. Water holding capacity and pH were increased in the ZLT group than the PC group. In conclusion, according to the results of growth performance, nutrients digestibility, bacteria counts, and meat characteristics, supplementation of the ZLT in broiler diet can alleviate HS.

Effects of Photoperiod on the Performance, Blood Profile, Welfare Parameters, and Carcass Characteristics in Broiler Chickens.

Simple SummaryThe physiology and behavior of birds, including poultry, are greatly affected by light. The poultry industry uses lighting regimens with long lighting periods to maximize the growth of broiler chickens. Rapid growth not only adversely affects the health of the birds, but also causes great stress. As animal welfare issues have become increasingly important, concerns regarding the current photoperiods in broiler production have increased. In this study, the effects of the photoperiod on the productivity and various welfare indicators on broiler rearing were investigated. Generally, the productivity and stress of broilers were both positively and negatively correlated with the photoperiod. The results showed that a photoperiod of 18L:6D was appropriate, when considering the growth and stress of broilers. The optimum photoperiod for broilers may vary in different feeding phases, with respect to performance and welfare. Therefore, continuous research is needed to establish the ideal light regimens under the consideration of the productivity and welfare of broilers.We studied the effects of photoperiods on the growth performance, blood profile, welfare parameters, and carcass characteristics of broilers. A total of 336 male broiler chicks (Ross 308) were randomly allocated into 4 treatments (84 birds per treatment with 4 replicates), based on the following lighting regimen: 24 h continuous light (24L), 18 h continuous light (18L:6D), 8 h continuous light (8L:16D), and intermittent light (4L:2D). Body weight and feed intake of 7- and 35-day-old broilers were measured. At 5 weeks of age, 12 birds per treatment were selected for blood collection and carcass analysis. Body weight, body weight gain, and feed intake were the lowest in the 8L:16D treatment (p < 0.05). The heterophil-to-lymphocyte ratio, aspartate aminotransferase, interleukin-6, and corticosterone levels in the 24L treatment increased significantly when compared to that in the 18L:6D treatment (p < 0.05). The footpad dermatitis score was significantly lower in the 18L:6D and 8L:16D treatments than in the 24L and 4L:2D treatments (p < 0.001). There was no significant difference in the carcass and meat characteristics, except for the shear force of breast meat (Pectoralis major), which was the lowest in the 8L:16D treatment (p < 0.05). These results indicate that a photoperiod of 18 h resulted in an improvement in the performance and welfare of birds and a simultaneous decrease in stress level. However, further research is needed to establish a lighting regimen that satisfies both the productivity and welfare requirements of broilers in different feeding phases.

Welfare implications for broiler chickens reared in an insect larvae-enriched environment: Focus on bird behaviour, plumage status, leg health, and excreta corticosterone.

The use of insect live larvae as environmental enrichment has recently been proposed in broiler chickens, but the concomitant administration of black soldier fly (BSF) and yellow mealworm (YM) has never been tested yet. Therefore, the present study aims to evaluate the effects of live BSF and YM larvae as environmental enrichments for broiler chickens by means of plumage status, behaviour, leg health, and excreta corticosterone metabolites (CM). A total of 180 4-day old male Ross 308 broiler chickens were randomly distributed in 3 experimental treatments (6 replicates/treatment, 10 birds/replicate) and fed for 35 days as follows: 1) control (C, commercial feed), 2) BSF: C + 5% of the expected daily feed intake [DFI] live BSF larvae and 3) YM: C + 5% of the expected DFI live YM larvae. Feathering, hock burn (HB) and footpad dermatitis (FPD) scores (end of the trial), as well as behavioural observations (beginning of the trial [T0] and every 11 days [T1, T2 and T3] during morning, larvae intake and afternoon) through video recordings, were assessed, and excreta samples collected to evaluate the CM. Feathering, HB and FPD scores, and excreta CM were unaffected by insect live larvae administration (p > 0.05). In the morning, the insect-fed birds displayed higher stretching, wing flapping, ground pecking (at T1 and T3), as well as lower preening (at T1 and T2), than the C group (p < 0.05). During the larvae intake, higher scratching, wing flapping and ground pecking, as well as lower stretching, preening and laying down, were observed in the insect-fed (scratching, stretching and laying down) or YM-fed (wing flapping, ground pecking and preening) groups than the C birds (p < 0.05). In the afternoon, insect live larvae administration increased wing flapping (YM) and laying down (BSF and YM), as well as decreased ground pecking (YM, p < 0.05). In conclusion, the administration of insect live larvae as environmental enrichment (especially YM) was capable of positively influencing the bird welfare through the stimulation of foraging behaviour, increase in activity levels, and reduction in bird frustration, without affecting the plumage status, leg health, and excreta CM.

Effect of dietary metallo-protease and Bacillus velezensis CE 100 supplementations on growth performance, footpad dermatitis and manure odor in broiler chickens.

ObjectiveThis study focused on the effect of dietary metallo-protease and Bacillus velezensis CE 100 on growth performance, carcass parameters, intestinal microflora, footpad dermatitis (FPD), and manure odor in broiler chickens.MethodsOne hundred-ten (two-day-old Ross 308) broiler chicks were randomly assigned to five groups with two replicate pens. The dietary treatments were divided to control, metallo-protease groups (A1, added with 0.1%; A2, added with 0.2%) and B. velezensis CE 100 groups (B1, added with 0.5%; B2, added with 1.0%).ResultsThe feed intake was decreased in A1 and B2 compared to the other group (p<0.05). The liver weight was lower in B2 than in A2 (p<0.05). The Salmonella in the cecum was decreased in A2 compared to control and A1 (p<0.05). However, the lactic acid bacteria were increased in all treatments (p<0.05). The litter moisture content was decreased in A2, B1, and B2 (p<0.05). The litter quality visual score was increased in all treatments (p<0.05). The FPD score and prevalence were reduced in all treatments (p<0.05). The (CH3)2S emission was decreased in all treatments (p<0.05).ConclusionThe present study indicated that both additives improve litter quality and reduce the incidence of FPD. These findings suggest that dietary metallo-protease and B. velezensis CE 100 have the potential to improve the broiler chickens’ welfare.

Objective scoring of footpad dermatitis in broiler chickens using image segmentation and a deep learning approach: camera-based scoring system

ABSTRACT 1. Footpad dermatitis (FPD) can be used as an important indicator of animal welfare and for economic evaluation; however, human scoring is subjective, biased and labour intensive. This paper proposes a novel deep learning approach that can automatically determine the severity of FPD based on images of chicken’s feet. 2. This approach first determined the areas of the FPD lesion, normal parts of each foot and the background, using a deep segmentation model. The proportion of the FPD for the chicken’s two feet was calculated by dividing the number of FPD pixels by the number of feet pixels. The proportion was then categorised using a five-point score for FPD. The approach was evaluated from 244 images of the left and right footpads using five-fold cross-validation. These images were collected at a commercial slaughter plant and scored by trained observers. 3. The result showed that this approach achieved an overall accuracy and a macro F1-score of 0.82. The per-class F1-scores from all FPD scores (scores 0 to 4) were similar (0.85, 0.80, 0,80, 0,80, and 0.87, respectively), which demonstrated that this approach performed equally well for all classes of scores. 4. The results suggested that image segmentation and a deep learning approach can be used to automate the process of scoring FPD based on chicken foot images, which can help to minimise the subjective bias inherent in manual scoring.

Partial Replacement of Animal Fat with Full-Fat Almond in Broiler Chicken Diets: Performance, Nutrient Digestibility, Blood Profile, Cecal-Fecal Microflora Composition, and Foot-Pad Dermatitis.

Simple SummaryRecently, as the price of grains in feed increases worldwide, interest in new raw materials that can replace protein and fat sources is increasing. This study evaluated the effect of full-fat almonds (FFA) on broiler nutrition. In the study, the formulation of FFA as a raw material showed higher growth performance than the basic feed. This seems to be due to the high digestibility of protein and fat and the promotion of intestinal health through the reduction of harmful microorganisms in the intestine. Therefore, FFA can be used as a high-quality protein and fat substitute as a raw material for broiler feed.The purpose of this study was to evaluate the effect of full-fat almonds (FFA) as an alternative protein and fat source for broiler feed on broiler productivity, nutrient digestibility, blood characteristics, cecal-fecal microflora, and foot-pad dermatitis (FPD). A total of 96, one-day-old broiler chickens (Arbor Acres) with initial body weight 41.61 ± 0.36 g were placed in 16 cages. In each trial, four treatments were set up: a basal diet partially replacing animal fat with FFA 0% (Control, CON), a basal diet partially replacing animal fat with FFA 1% (T1), a basal diet partial replacing animal fat with FFA 2% (T2), a basal diet partially replacing animal fat with FFA 4% (T3). The experiment was conducted for a total of 4 weeks. Feed conversion ratio (FCR) was higher (p < 0.05) in the T3 group of broilers at weeks 0 to 1 than in the CON group of broilers. From weeks 3 to 4, and for the entire experimental period, FCR was lower (p < 0.05) in the T3 group of broilers than in the CON and T1 groups of broilers. The apparent ileal digestibility (AID) of the ether extract (EE) was higher (p < 0.05) in the T3 group than in the other treatment groups, and AID of crude protein (CP) was higher (p < 0.05) in the T3 group than in the CON group. The apparent total tract digestibility (ATTD) of EE was lower (p < 0.05) in the CON group than in the other treatment groups, and the ATTD of CP and energy was higher (p < 0.05) in the T3 group of broilers than in the CON group of broilers. The AID and ATTD of total amino acids were higher (p < 0.05) in the T3 group than in the other treatment groups. Blood cholesterol levels were lower (p < 0.05) in the T3 group of broilers than in the CON and T1 groups of broilers, and higher (p < 0.05) in the CON group of broilers than in the T2 and T3 groups of broilers. The amount of E. coli in the cecal and fecal was lower (p < 0.05) in the T3 group than in CON and T1 groups. FPD score was higher (p < 0.05) in the T3 group of broilers than in the CON group of broilers. In conclusion, replacing a partial of animal fat with at least 4% FFA in broiler diets can increase growth performance and nutrient digestibility in broiler nutrition.

Dietary Resin Acid Concentrate Improved Performance of Broiler Chickens and Litter Quality in Three Experiments.

Simple SummaryResin acids are antimicrobial and anti-inflammatory compounds derived from spruce and pine trees. In a previous study, in which a mixture of resin acids and fatty acids from pine and spruce trees was added to the feeds of broiler chickens, they grew heavier, and their footpads stayed in better condition. Here, three separate trials were conducted to study whether dietary resin acids without the fatty acids have the same effects on broiler chickens. Broiler chicken diets were amended with different dosages of a resin acid concentrate product. Bird weight gain increased, and the efficiency of broiler production was improved when the resin acid concentrate was added to the diet at 125–250 g/ton. The litter material of pens stayed drier in resin acid concentrate groups, which may indicate better intestinal condition of the birds. However, the footpad condition was unaffected by the experimental diets. The results suggest that the resin acid concentrate improves bird weight gain and the efficiency of broiler production. Therefore, the resin acid concentrate may be a promising feed additive for broiler chickens.Dietary coniferous resin acids have previously been suggested to support the intestinal integrity of broiler chickens by reducing mucosal collagen degradation. The present study examined the effects of resin acid concentrate (RAC) on broiler performance and litter quality. In trial 1, RAC was added to diets at 0, 125, 250, or 1250 g/ton, while in trials 2 and 3, RAC dosing was 0 or 175 g/ton. Bird weight, feed consumption, mortality, feed conversion ratio (FCR), European Efficiency Index (EEI), litter moisture, and footpad dermatitis (FPD) lesions were measured. In trial 1, RAC at 125 and 250 g/ton improved weight gain and EEI, while RAC at 1250 g/ton group did not differ from control. Feed consumption, FCR, FPD scores and mortality were similar in all treatments, but litter quality was improved by all doses of RAC. In trials 2 and 3, RAC increased the final weight of birds, improved FCR, EEI, and litter quality, but had no effects in other parameters. In summary, RAC at 125–250 g/ton improved bird performance and thus shows promise as a feed additive. The dryer litter in RAC treatments may suggest improved intestinal condition as a response to in-feed resin acids.

Effect of high stocking density and dietary nano-zinc on growth performance, carcass yield, meat quality, feathering score, and footpad dermatitis in broiler chickens

In this study, effect of dietary nano-Zn and stocking density on performance, carcass yield, meat quality, and feathering score of broiler chickens was assessed at 42 d of age. Experimental treatments consisted of a 2 × 2 factorial arrangement of stocking density (low = 12 broiler/m2 and high = 18 broiler/m2) and dietary Zn form (inorganic or nano). Four hundred eighty 1-d-old male broiler chickens were randomly assigned to 4 treatments with 8 pens in each treatment having 15 chickens per pen. Corn-soybean meal diets were formulated for starter, grower, and finisher phases. The high stocking density (HSD) negatively affected body weight gain (BWG) and feed intake (FI) of broiler chickens at 24 to 42 and 0 to 42 d of experiment (P < 0.05) but did not affect feed conversion ratio (FCR). The BWG (d 10 to 24, d 24 to 42, and d 0 to 42) and FI (d 10 to 24 and d 0 to 42) were negatively affected by dietary nano-Zn whereas, FCR was poor at 10 to 24 d only (P < 0.05). Dietary nano-Zn lowered the BWG at d 24 to 42 (P = 0.028), and FI of LSD and HSD treatments at d 24 to 42 and 0 to 42 (P = 0.019 and P = 0.028, respectively) compared to broiler chickens of LSD group fed inorganic Zn. Neither HSD nor nano-Zn had any effect on the relative yields of carcass, breast, and thigh. Meat quality traits were not affected by either HSD or nano-Zn. There were no differences between groups about back feathering score. However, HSD negatively affected wing feathering score (P < 0.05), and the worst score was observed in HSD dietary inorganic Zn group (P < 0.01). The HSD increased the footpad dermatitis (FPD) scores of chickens at d 24 and 42 (P < 0.01). Dietary nano-Zn reduced the FPD scores on d 24 (P < 0.05) but did not affect FPD on d 42. In conclusion, HSD negatively affected growth performance, wing feathering score, and FPD score in broiler chickens. Dietary nano-Zn suppressed the growth performance of broiler chickens subjected to HSD and did not affect the feathering, however, reduced the FPD score in broiler chickens.

Effects of Different Swimming Pool Conditions and Floor Types on Growth Performance and Footpad Dermatitis in Indoor-Reared White Roman Geese.

Simple SummaryThe goose industry provides meat and down to the food and textile processing industry and is the third-largest poultry industry in Taiwan after the chicken and duck industries. After the avian influenza virus (HPAIV) pandemic in 2015, most poultry farms in Taiwan have been restricted to closed birdhouses to improve biosafety. However, indoor-raised poultry may experience footpad dermatitis problems. We studied the effects of providing a swimming pool and different floor types on the growth performance and footpad dermatitis score for indoor-reared White Roman geese to reduce the risk of footpad dermatitis. Our data indicated that the incidence of footpad dermatitis was decreased during the feeding period in geese supplied with a swimming pool. Our findings may help improve animal welfare in modern waterfowl production by having the geese express their natural behaviors with water.Footpad dermatitis (FPD) is a major foot disease in modern poultry production, and it affects both poultry health and animal welfare. It refers to inflammation and necrotizing lesions on the plantar surface of the footpads and toes. We investigated the effects of providing a swimming pool and different floor types on growth performance and FPD score in indoor-reared White Roman geese. Forty-eight male and 48 female White Roman geese were randomly allocated to pens with or without a swimming pool and with either mud or perforated plastic floor and reared from 15 to 84 days of age. Growth performance measurements included feed intake (FI), weight gain (WG), and feed conversion ratio (FCR). FI, WG, and FCR were significantly decreased at various growth periods in geese provided with a pool. Lower WG and bodyweight for the perforated plastic floor group were found at 15–28 and 28 days of age, respectively. The geese reared on the perforated plastic floors without a pool had higher FPD scores at 70 and 84 days of age than those with other rearing conditions. A higher incidence of FPD score 1 was observed in geese raised without a pool. In conclusion, providing a pool can improve footpad health in indoor-reared White Roman geese but may not benefit growth performance.

Effect of Bacillus pumilus supplementation on performance, intestinal morphology, gut microflora and meat quality of broilers fed different energy concentrations

In this research trial, the effects of the dietary supplementation with a probiotic were evaluated in broiler chickens fed diets with standard or reduced (-4%) metabolizable energy concentration. A total of 640 day-old Ross-308 male chicks were allocated into four equal treatments with ten replicates each (16 chicks per pen). The control treatment (D1) was fed with the basal diets (starter 1−10 days, grower 11−24 days, and finisher 25−35 days). The second treatment (D2) was fed diets containing 4% lower metabolizable energy, compared to the control. The third treatment (D3) and fourth treatment (D4) were fed the diets of the first and second treatments, respectively, further supplemented with a probiotic (Bacillus pumilus) at the level of 7 × 108 CFU (viable spores)/kg feed. All diets were supplied in mash form, and feed and water were provided to the birds ad libitum. The examined parameters included performance, gut histology, gut microbiota populations, welfare parameters (diarrhea and foot pad dermatitis scores), bedding material quality and carcass quality parameters. Two-way analysis of variance method (energy concentration x probiotic supplementation) was used for the statistical analysis. The main significant (P ≤ 0.05) effects of the lower energy diets were: decreased final body weight and weight gain; decreased ileum villus height and number of goblet cells; decreased counts of Bacillus spp in the ileum and the ceca; increased diarrhea score (day 24) and footpad dermatitis score (days 24 and 35); increased bedding material moisture and nitrogen concentration; change of carcass composition by lowering the fat percentage while increasing moisture and protein percentages; improved meat oxidative stability under refrigerated storage up to 4 days. Furthermore, the main significant (P ≤ 0.05) effects of the Bacillus supplementation were: increased final weight and weight gain, and improved feed conversion ratio; increased ileum villus height and number of goblet cells; increased counts of Lactobacillus and Bacillus spp in the ileum and the ceca; improved diarrhea score (days 10 and 24) and footpad dermatitis score (days 10 and 24); decreased bedding material moisture and nitrogen concentration; improved meat oxidative stability under refrigerated storage up to 4 days. In conclusion, the results showed that this probiotic supplementation of the diet can support broiler performance, welfare and meat quality. In addition, as treatment D4 had similar body growth and feed conversion with treatment D1, it appears that the probiotic supplementation had an energy sparing effect on the energy-diluted diets.

Effect of humate supplementation to feed and/or litter on performance, intestinal viscosity, litter quality, and occurrence of footpad dermatitis in broilers fed barley-based diets.

We investigated the effect of in-feed and/or in-litter supplemental humate against footpad dermatitis (FPD) in broilers fed diets based on barley. Three hundred and sixty 1-day-old Ross 308 broiler chickens were randomly distributed to 24 floor pens (4 treatments, each consisting of 6 replicate pens; 15 chickens per pen) as a completely randomized design with 2 × 2 factorial arrangement of two levels of supplemental humate in feed (0 and 1g/kg feed) and litter (0 and 5g/kg litter). Growth performance, intestinal viscosity, litter quality, and incidence and severity of FPD in broilers were measured. In addition, malondialdehyde (MDA) and superoxide dismutase (SOD) levels were determined in blood and footpad tissues of broilers with different FPD scores. The results revealed that there was no interaction between humate supplementation to feed and litter. Neither dietary nor litter supplementation of humate had a significant effect on growth performance, intestinal viscosity, litter quality, and occurrence of FPD. And also, MDA and SOD levels in serum and footpad tissue did not affect by either dietary or litter supplementation of humate. The presence of FPD (score 1) had no effect on MDA and SOD levels in serum, however, increased the MDA and SOD levels (P < 0.001, P = 0.001, respectively) in footpad tissue of broilers. The intestinal viscosity did not differ between FPD scores 0 and 1. In conclusion, findings of this experiment suggest that humate supplementation to feed and litter did not alleviate FPD development in broilers fed diets based on barley. In addition, the presence of FPD lesions increases the MDA and SOD levels in the footpad tissues.