Food Trees Research Articles

Towards responding to climate change: practices in Iranian healthcare centers

The aim of this study was to investigate the prevailing practices suitable for Iranian healthcare centers to address climate change and to rank them in terms of priority. This paper, a mixed-methods study conducted in 2023, comprised a scoping review and a thematic analysis covering the period from 2000 to 2023 as the first phase of the study. In this regard, PubMed, Scopus, ProQuest, and the Cochrane Database of Systematic Reviews were searched. In the second phase, the authors engaged a group of experts to prioritize the most important practices suitable for Iranian healthcare centers to address climate change using the Grey MEREC-MABAC integrated approach. The scoping review resulted in 38 studies, and the thematic analysis revealed 11 primary categories of practices employed to address climate change. Subsequently, the Grey MEREC-MABAC approach indicated that the themes of 'Green Environment' (CC = 0.0929), 'Green Equipment, Waste, and Water Management' (CC = 0.0677), and 'Green Energy' (CC = 0.0653) were identified as having the highest priority among the practices deemed most important in the literature for addressing climate change within Iranian healthcare centers. The study proposed that practices or activities promoting biodiversity and environmental sustainability, such as organic food farming and tree planting, should be considered paramount approaches in Iranian healthcare centers to combat climate change.

Public Food Trees’ Usage and Perception, and Their Potential for Participatory Edible Cities: A Case Study in Birjand, Iran

Public food trees are increasingly popular among researchers, urban planners, and citizens for their diversity of social, provisional, and environmental benefits. However, more research is needed to determine how to overcome their barriers. Here we used a qualitative approach to explore the usage and perception of public fruit trees and attitudes towards public usage and participation in two urban green spaces harboring fruit trees in Birjand, Iran: Tohid Park and Akbarieh Garden. Semi-structured interviews were conducted with twelve visitors to both spaces, four administrators, and eight workers. Almost all visitors had great personal experience and interest in picking fruit; nevertheless, usage in these green spaces was low, partly due to social norms. Almost all visitors appreciated the public fruit trees for the diverse pleasures that they provide (sensory, emotional, cognitive, experiential, social). Ten also had a high interest in participating in their management, especially in Tohid Park due to its greater social impact. Education and access limitation were suggested by some as important to reduce tree damage and maximize production. Our findings expand our understanding of how citizens relate to public urban fruit trees and can be involved in co-management schemes, thereby contributing to building smart and participatory edible cities.

Natural variation in the nutritional composition of African baobab (Adansonia digitata L.) from two ecological sites in Northern Malabar, Kerala, India

Adansonia digitata L., commonly known as the African baobab, is an important plant within the Malvaceae family. It is a highly nutritious forest tree and holds a special place in several African countries. Despite its prevalence in Africa, its presence is very limited in India, and only a few individual plants of this species are reported from different parts of India, including Kerala. This study focuses on the plants growing at Ajanur and Thalassery in the Kasaragod and Kannur districts of Kerala, respectively. This study reports natural variation in phytochemicals, minerals, and the proximate components of its flowers and leaves at these locations. The results of this study show that total carbohydrate (28.79 %), crude fibre (10.76 %), and protein (53.09 %) content was higher in leaves at Ajanur. In contrast, chlorophyll a, b, and carotenoid content were highest in leaves at Thalassery and showed a value of 39.03 mg/g, 16.65 mg/g, and 13.21 mg/g, respectively. The highest quantity of elements viz. Ca, Zn, K, Fe, Cu, Ni, and Cr were reported in leaves from Thalassery, whereas Mg and Mn were abundant in the leaves from Ajanur. Flowers from Ajanur demonstrated a superior nutritional index, while Thalassery leaves exhibited a higher mineral index. Assessment of antioxidant activity indicated the highest DPPH scavenging activity in the leaves from Ajanur (IC50 = 102.57 μg/mL) and the highest ABTS scavenging activity in its flowers from Thalassery (IC50 = 62.74 μg/mL). The differences in nutritional components and antioxidant properties of the baobab from these two ecological sites underscore the role of ecological factors in its natural variation. Although it is an important forest food tree in the African continent, its potential contribution towards food security from the Indian subcontinent remains untapped.

Etnobotani Tikung Masyarakat Melayu Dusun Batu Rawan Kabupaten Kapuas Hulu dalam Budidaya Madu Secara Tradisional

Wild Honey is produced directly from the hives of Apis dorsata bees or wild bees that live in forest areas. Wild Honey is produced through traditional methods that utilize plants and are not livestock. Plants that are utilized in the form of plant wood as artificial nests called tikung and flowers from bee food trees. The purpose of this study was to determine the types of plants as tikung wood and bee food trees and document the knowledge of traditional honey farmers about the ethnoecology of Batu Rawan Hamlet, Kapuas Hulu Regency. This study used purposive sampling method in determining respondents and 15 respondents were obtained, then continued with semi-structured interviews. Data analysis was conducted with citation frequency. The results showed that there were 5 species of tikung wood and 15 species of bee food trees belonging to 12 families. Fagraea fragrans, Shorea belangaran, Litsea resinosa, Gluta renghas, Shorea sp., are plant species used as tikung wood trees, the dominant species reported by respondents is Litsea resinosa which is 93.3%. Mesua hexapetala, Syzygium claviflorum, Barringtonia acutangula, Ternstroemia sp., Carallia brachiata, Teysmanniodendron sarawakanum, Syzygium sp., Gomphia serrata, Eugenia sp., Syzygium inophyllum, Timonius flavescens, Ixora mentangis, Syzygium attenuatum, Memecylon edule, Crudia teysmannii are plant species used as bee fodder trees, bee fodder tree species reported dominantly by respondents are Barringtonia acutangula species which is 100%.

Koala density, habitat, conservation, and response to logging in eucalyptus forest; a review and critical evaluation of call monitoring

ABSTRACT This study is the second in a series that examines the habitat requirements and response to logging of koalas (Phascolarctos cinereus) inhabiting tall eucalypt forests of north-east NSW. It presents the findings of koala population and habitat monitoring surveys in Pine Creek State Forest and Bongil Bongil National Park using a combination of call-counting and direct observation (spotlighting). The 6400 ha study area was mapped into 6 zones of increasing koala habitat quality by ground survey of forest structure and floristics on a 200 m grid. The accuracy of habitat definition and mapping was tested by stratified transect counts of koala calls and sightings over two consecutive years (1997–98). Average koala density increased steeply and significantly, from 0.02 – 0.20 koalas/hectare, with increasing mapped habitat quality based on increasing forest age, structural complexity, local food tree species diversity, history of prior koala occurrence and decreased past logging intensity. This relationship was driven primarily by breeding females, with the number of male koala calls weakly or uncorrelated with koala sightings and mapped habitat quality. Male koalas were more widely and uniformly distributed than females, including areas of low quality, plantation, and intensively logged forest. This finding explains the discrepancy between our results and those of other recent studies which concluded that koalas are tolerant of intensive logging based on modelling of calling male koalas and reliance on an untested assumption that male calling is indicative of female breeding success. Koala density in a subset of the highest quality habitat was relatively stable at 0.28 koalas/ha (3 hectares/koala) over the long term (1997–98 and 2012–2023). Key characteristics of the forest koala population, including low stable density, large home ranges, preference for high food tree diversity and locally unique food trees (including Allocasuarina torulosa and Syncarpia glomulifera), are not adequately explained by existing koala habitat models. We present a new paradigm to explain regional variation in koala distribution, habitat and foraging preferences based on variations in foliage chemistry (toxicity and nutritional value) determined by the duration and stability of local plant-koala interactions in response to past fire, hunting, predation and logging disturbance history. We hypothesize that koala density in stable forest populations is regulated at low levels by a combination of selected and induced increases in leaf toxicity and decreases in leaf nutrition that limit koala browsing to benign levels of about 1-2% of annual leaf production. Large home ranges, complex mature forest structure, high food tree diversity and a specialized or diverse gut microbiome may be essential to allow females to rotate and change food trees frequently to minimize induced toxicity and select individual leaves with sufficient nutrients to support breeding and lactation with minimal risk of predation. High density koala populations (> 0.6/ha) occur primarily in areas where koalas have been introduced or re-introduced to planted habitats and natural areas where aboriginal hunters and dingoes were historically present but are now absent, and where food trees have not been selected for resistance to koala browsing pressure.

The genomes of seven economic Caesalpinioideae trees provide insights into polyploidization history and secondary metabolite biosynthesis

The Caesalpinioideae subfamily contains many well-known trees that are important for economic sustainability and human health, but a lack of genomic resources has hindered their breeding and utilization. Here, we present chromosome-level reference genomes for the two food and industrial trees Gleditsia sinensis (921 Mb) and Biancaea sappan (872 Mb), the three shade and ornamental trees Albizia julibrissin (705 Mb), Delonix regia (580 Mb), and Acacia confusa (566 Mb), and the two pioneer and hedgerow trees Leucaena leucocephala (1338 Mb) and Mimosa bimucronata (641 Mb). Phylogenetic inference shows that the mimosoid clade has a much higher evolutionary rate than the other clades of Caesalpinioideae. Macrosynteny comparison suggests that the fusion and breakage of an unstable chromosome are responsible for the difference in basic chromosome number (13 or 14) for Caesalpinioideae. After an ancient whole-genome duplication (WGD) shared by all Caesalpinioideae species (CWGD, ∼72.0 million years ago [MYA]), there were two recent successive WGD events, LWGD-1 (16.2–19.5 MYA) and LWGD-2 (7.1–9.5 MYA), in L. leucocephala. Thereafter, ∼40% gene loss and genome-size contraction have occurred during the diploidization process in L. leucocephala. To investigate secondary metabolites, we identified all gene copies involved in mimosine metabolism in these species and found that the abundance of mimosine biosynthesis genes in L. leucocephala largely explains its high mimosine production. We also identified the set of all potential genes involved in triterpenoid saponin biosynthesis in G. sinensis, which is more complete than that based on previous transcriptome-derived unigenes. Our results and genomic resources will facilitate biological studies of Caesalpinioideae and promote the utilization of valuable secondary metabolites.

Diversity of specific indicators of walnut fruits during selection using various methods

Walnut (Juglans regia L.) is a particularly valuable plant for humans. This is due to its multifaceted beneficial properties and qualities as a food, medicinal, technical, recreational and environmental tree species. The main goal of nut breeding is to obtain high-quality fruits. In the Russian Federation for the selection of individuals that provide such products, two methods are mainly used: method 1 – «Program and method for breeding walnuts» (Sukhorukikh et al., 2007) and method 2 – «Program and method for the study of fruit, berry and nut crops» (1999). The purpose of the study is to study the diversity of promising forms of walnut identified on the basis of methods 1 and 2 in terms of taste, kernel extractability and the overall score of the breeding value (quality) of the fruit. Other characteristics assessed identically or similarly are not considered. The object of the research was the literature data of the authors assessing the quality of nuts using methods 1, 2. For statistical processing, the Stadia 8.0/prof. program was used, and Microsoft Excel was used for graphical construction. Diversity was assessed using coefficients of variation and indices of distributed rank diversity (IRRR) based on known methods. It was established that the coefficients of variation of the studied indicators, assessed using various methods, did not have a significant statistical difference, and the selection gradations of taste and kernel extractability were equally distributed. When assessed using method 1, the statistical distribution of the total score of the selection value of fruits had a normal distribution, when using method 2 it differed from normal. When assessing diversity using IRRR values, method 1, compared to method 2, turned out to be more sensitive and provided greater diversity of the allocated gene pool in terms of the overall score of the breeding value of walnut fruits. The results can be used in walnut breeding.

Provisioning services decline for both people and Critically Endangered wildlife in a rainforest transformation landscape

Abstract The loss and degradation of forests and other ecosystems worldwide threaten both global biodiversity and the livelihoods of people who use natural resources. Understanding how natural resource use impacts landscape provisioning services for both people and wildlife is thus critical for designing comprehensive resource management strategies. We used data from community focus groups, botanical plots and an inventory of plant species consumed by the Critically Endangered red‐ruffed lemur (Varecia rubra) to assess the availability of key provisioning services for people and endemic wildlife on the Masoala Peninsula, a rainforest transformation landscape, in northeastern Madagascar (Masoala National Park and 13 surrounding communities). We constructed Poisson regression mixed models to evaluate the impact of community factors (i.e. community population size, plot distance to community) and changes over time on the count and species richness of timber trees, medicinal plants and red‐ruffed lemur food trees within botanical plots. Over three‐quarters of all plant species could be used for at least one purpose by local communities (n = 238 species). Of the 59 V. rubra food tree species, only 15% had no reported human use. Timber and ruffed lemur food tree availability declined both with community population size and time and were predicted to be lower outside of Masoala National Park. In contrast, medicinal plant availability was not strongly predicted by any tested factors. Provisioning service availability also differed strongly across sites, suggesting that additional, untested proxies of human pressure likely also have an effect. Our results highlight the importance of evaluating natural resource availability from a community‐based perspective and by resource purpose to inform forest landscape restoration efforts that can support both people and wildlife. Read the free Plain Language Summary for this article on the Journal blog.

Beyond the metropolis: street tree communities and resident perceptions on ecosystem services in small urban centers in India

Abstract Although 41% of the global urban population lives in smaller cities, the nature and trajectories of urban nature, ecosystem services and human-nature connections in smaller centers is relatively unknown. While megacities in developed countries move towards biophilic urban planning and participatory decision making, basic information on tree communities and their perceived services is a bottleneck in achieving these goals in such small urban centers. Through a combination of field measurements (258 transects, 931 trees) and semi-structured interviews (497 individuals), we assessed the street tree community and resident perceptions of ecosystem service values in two coastal cities on the west coast of India that are under rapid change. We found that mean street tree density is low in both cities, and corresponds to perceptions of recent change in tree cover (−28% in Kochi, −11% in Panjim). The street tree community in both cities were dominated by ornamental avenue trees such as Albizia saman and Peltophorum pterocarpum, but native coastal species like Cocos nucifera, Terminalia catappa and Thespesia populnea were also common. Despite recent urban growth, residents in both cities reported low value of trees for food, fodder and medicine, but high value for regulating services like shade and water. Moreover, we found strong evidence for aesthetic and cultural values of trees in both cities. Our study establishes critical baselines for biophilic planning in these small urban centers towards urban sustainability in India.

Yams (Dioscorea spp.) in shellmounds and swiddens: ancient history in Babitonga Bay, Santa Catarina State, southern Brazil

BackgroundIn Babitonga Bay, southern Brazil, records of yam consumption exist among shellmound builders from at least 4000 years ago. Shellmounds (sambaquis) are anthropogenic structures in the form of mounds with layers of shells associated with other faunal remains, as well as with charcoal, artefacts and burial. Larger sambaquis are considered to be funerary monuments. The indigenous Jê and Guarani people also lived in the region before the European invasion and cultivated yams. Currently, exotic and domesticated yams are cultivated in the region by farmers. Our aim is to describe the long-term history between the people and Dioscorea in the Babitonga Bay region based on its consumption and occurrence in shellmounds and swiddens.MethodsSurveys of Dioscorea spp. and host were carried out in the vegetation of shellmounds and in the surrounding area using visual detection through intensive searches in transects using the walking method. The survey of Dioscorea species used and cultivated in the precolonial, colonial and current periods was carried out based on the literature. In the present study, only Dioscorea trifida cultivations were recorded.ResultsDioscorea cayennensis, Dioscorea chondrocarpa, Dioscorea dodecaneura, Dioscorea laxiflora, Dioscorea olfersiana, and Dioscorea scabra, all recorded in associated vegetation of shellmounds, in different combinations of the species. In swiddens, D. trifida is most common, followed by Dioscorea alata and, to a lesser extent, Dioscorea bulbifera and D. cayennensis. Records of food use prevail, but they are used as medicinal plants. Yams are integrated on anthropogenic soils of shellmounds and in swiddens in monoculture systems or in intercropping with Zea mays or Colocasia esculenta. The presence of exotic food trees and D. cayennensis in some shellmounds indicates the influence of colonizers on the composition of the vegetation. In sambaquis, there are overlapping processes of construction of cultural niches by different human groups at different times.ConclusionsThe sambaquis and the associated vegetation and swiddens form part of a domesticated landscape. The native species of Dioscorea recorded in shellmounds and surrounding vegetation do not depend on human action to perpetuate themselves in the environment. However, this does not rule out human influence in the past, but it does not indicate horticulture among the Sambaquianos. Greater investment in genetic, archaeobotanical and ethnobotanical research can contribute to a better understanding of the relationship between people and yams over thousands of years.

Unlocking the potential of sheep and goat genetics in developing countries

Sheep and goats are vital components of agricultural landscapes in developed and developing countries, with Asia and Africa jointly hosting 94% and 77% of global goat and sheep populations, respectively. Despite their importance, these regions grapple with heightened food insecurity, transitioning from traditional pastoralism to cultivating cereal crops and food trees. Decreased rangelands have led to including grains in animal feed, escalating production costs. The once-prevalent mixed farming practice, involving three or more species, regulating livestock systems and farm-level production strategies, has declined. While successful selection and crossbreeding initiatives exist, many developing countries lack cohesive breeding strategies and programs to boost sheep and goat productivity. Farmer organizations often lack empowerment for efficient meat and milk production. Addressing these challenges requires exploring pathways to unlock the genetic potential of these animals. Proposed solutions advocate for an integrated approach involving "selection" and "crossbreeding" strategies, with organized consortiums comprising breeders, community-based associations, commercial enterprises, or governmental agencies. For successful implementation, international organizations must play a crucial role by providing essential support and resources. This collaborative effort holds the potential to enhance resilience, sustainability, and overall sheep and goats productivity, contributing to the global fight against food insecurity.

Ecological conditions of Javan langur (Trachypithecus auratus É. Geoffroy Saint-Hilaire, 1812) in Sokokembang Forest (Central Java, Indonesia) through distribution and food preferences

Javan langur (Trachypithecus auratus) is endemic to Java Island with vulnerable conservation status according to IUCN. The study aimed to determine the distribution and food preferences of Javan langurs in Sokokembang Forest as part of understanding ecological conditions. The research used strip transect and concentration count methods, sampling the distribution of food using accidental sampling and recording data on the utilization of food plants with rapid assessment. The results showed that the types of food consumed by javan langurs were 14 types of plants, which were scattered at 6 points on five transect lines. The percentage of preferred plants was (Pangium edule) 29.4%, (Artocarpus elasticus) 17.6%, (Dysoxylum excelsum) 11.8%, (Pometia pinnata) 8.8%, (Cinnamomum iners) 5.9%, and others 2.9%. The average vertical space utilization of javan langurs during feeding activities was 17.3 m (stratum C) with a percentage of 70.6%. The percentage of tree crown space usage pattern of javan langur in CII (36.1%), CI (19.4%), BII (13.9%), AI (11.1%) BII, and CIII (8.3%), AII (2.8%). Food type and tree crown space are important parts of the presence of Javan langurs in Sokokembang.

ECOLOGY OF HORNBILL FOOD TREES AT BUKIT BARISAN SELATAN NATIONAL PARK

Hornbills play an important role in maintaining tree gene flow among fragmented populations in forests. But hornbill populations in Asia have been declining mainly due to loss of forest cover and poaching. The presence of hornbills in a forest is highly dependent on environmental factors such as the condition of food trees in their habitat. The purpose of this study was to determine the spatial distribution, dispersion patterns, density, and diversity of hornbill food trees at Sukaraja Atas Resort (SA) and Balik Bukit Resort (BB) of Bukit Barisan Selatan National Park (BBSNP). We investigated a total of 10 transect (50 survey plots) for both SA and BB by collecting data on all types of hornbill food trees with a minimum diameter of 10 cm at breast height (DBH). We recorded 64 hornbill food tree species with a total of 98 individuals, which have a clumped and uniform dispersion pattern. This study showed that Lauraceae dominated in both resorts with the highest relative density. The results of the diversity index show that 9 transect have moderate diversity whereas 1 transect has low diversity; there was no significant difference between SA and BB. It can be concluded that the existing food trees in SA and BB may support the hornbill populations.

Impacts of bushfire on the Glossy Black-Cockatoo Calyptorhynchus lathami and its single food source in eastern Victoria

The Glossy Black-Cockatoo Calyptorhynchus lathami eats only unripened seeds extracted from green cones of Allocasuarina and Casuarina trees. This specialised feeding habit results in Glossy Black-Cockatoos leaving unique evidence of having fed in a tree (i.e. feeding sign). The frequency of feeding sign beneath Black Sheoak Allocasuarina littoralis trees in East Gippsland, Victoria, was used as an index of the presence of Glossy Black-Cockatoos before and after intense and widespread bushfires during the summer of 2019–2020. Species distribution models for the cockatoo and its major food tree in the region were used to define 175 survey points in forested parts of East Gippsland. Survey points were visited in mid 2019, to score the presence or absence of Black Sheoak and Glossy Black-Cockatoo feeding sign. Following the 2019–2020 bushfires, all sites where Black Sheoak was present pre-fire were revisited twice within the following 28 months to search for feeding sign and score the extent of regeneration of Black Sheoak. Across all sites, the frequency of sites with feeding sign effectively halved between the pre-fire survey and first post-fire survey, then halved again between the first and second post-fire surveys, an overall decline of 76%. After 25–28 months, regeneration of Black Sheoak, in the form of either basal sprouting of established trees or seed germination, was observed at 80.6% of burnt sites, with a tendency for highest rates of regeneration at lower-fire- severity sites and for suckering to be more common than seedlings at higher-fire-severity sites. These results highlight the vulnerability of the Glossy Black-Cockatoo to reductions in the availability of its specialised food. The greatest threat to food security stems from increased frequency and intensity of fire, as predicted for the region by climate change models.

Research progress on the effects of thermal processing on different food allergen proteins

Food allergy is a global public health and food safety problem, and the removal or attenuation of allergenic proteins through food processing has historically been a focus in the field of food science. This paper introduces the effect of thermal processing technology on allergen protein sensitization of different foods (milk, peanuts, eggs, tree nuts, and aquatic products). Although processing cannot completely eliminate the allergen allergenic potential, the effects can be minimized by the selection of processing methods and processing parameters. It is hoped that this paper can provide a certain reference for the research and development of hypoallergenic or desensitized foods to ensure that desensitized foods can be obtained without affecting the nutritional value of foods to meet the normal dietary needs of people with food allergies. In addition, due to the vast territory of China, residents in different regions have different eating habits and lifestyles for people with food allergies, which makes it very difficult for people with food allergies to carry out dietary interventions. Therefore, one of the indispensable and important missions of the modern food industry is to solve the problem of dietary restrictions for people with food allergies, and the latest research results summarized in this article will help this.

Characteristics of sleeping sites and sleeping trees selected by Celebes crested macaques (Macaca nigra) in Tangkoko, North Sulawesi, Indonesia

Abstract. Qomariyah IN, Perwitasari-Farajallah D, Iskandar E, Berthier JM. 2023. Characteristics of sleeping sites and sleeping trees selected by Celebes crested macaques (Macaca nigra) in Tangkoko, North Sulawesi, Indonesia. Biodiversitas 24: 6200-6208. Research on the selection of sleeping sites is imperative in providing information on the fitness and survival of a species. We investigated the characteristics of the sleeping sites and the sleeping trees selected by wild Celebes crested macaques (Macaca nigra Desmarest 1822) in North Sulawesi, Indonesia, as well as their spatial and temporal use. The study was conducted in two groups of macaques for nine months (187 study days) from September 2020 to June 2021. These macaques avoided using the same sleeping site on consecutive nights. Two predator encounters (Python reticulatus Schneider 1801) were recorded near the sleeping sites, but pythons caught no monkeys during the study period. The macaques used 58 trees from 13 species as sleeping trees, of which 79% of the sleeping trees were also food trees. M. nigra chose sleeping trees with higher diameter, height, and a bigger Important Value Index than the surroundings. The average distance between sleeping trees and feeding trees in the morning and evening were 203 m and 127 m, respectively. These results suggest that optimal foraging strategy and predator avoidance are important factors in selecting sleeping sites, providing a broader understanding of the habitat preferences of the Critically Endangered M. nigra.

Feeding ecology of a lesser-known arboreal giant: Grizzled Giant Squirrel (Ratufa macroura), Southern Western Ghats, India.

Animal dietary choices help us understand a species' feeding behaviour and are particularly relevant in conservation management. The aim of this study was to gather knowledge on dietary choices and the foraging behaviour of the Grizzled Giant Squirrel (Ratufa macroura) in Chinnar Wildlife Sanctuary, Southern Western Ghats, Kerala, India. The objectives were in particular to determine the food composition, seasonal fluctuations in food selection and feeding technique. Through an observational sampling method, focal animal sampling, the Grizzled Giant Squirrel in Chinnar Wildlife Sanctuary was found to feed on 30 plant species belonging to 18 families. The most used plant family was Fabaceae, with eight species, followed by Moraceae (four species) and Anacardiaceae (two species). The food species consumed included 22 trees, four climbers, one liana, one paraphyte, one shrub and one succulent species. The squirrel spent the most time feeding on Bauhinia racemosa (19.79%), followed by Tamarindus indica (14.08%) and Nothopegia beddomei (9.89%). The squirrel's diet choice was primarily influenced by the availability of food tree species and food resources rather than the season and nontree species were also found in the diet of Grizzled Giant Squirrel. Although the Grizzled Giant Squirrel exhibits some plasticity in its dietary choices, the available diversity of mature trees and plants as food sources appears to be important for its conservation in the fragmented riparian forest of the Western Ghats in Southern India.

Adoption of Agroforestry as a Climate Smart Agriculture Practice among Smallholder Farmers in Kakamega County, Kenya

Climate Smart Agriculture (CSA) technologies help guide actions needed to transform and reorient agricultural systems to effectively support development and ensure food security by increasing farmers’ resilience to climate change. Agroforestry has been vouched for as one of the best CSAs because it provides ecosystem services such as water quality enhancement and prevents land degradation. This study sought to establish and document the adoption of agroforestry as a CSA practice among smallholder farmers in Kakamega County. Stratified sampling was used to select six sub-counties to represent the county's various agroecological zones and regions for the research sample. A combination of purposive and snowball sampling was used to select 428 smallholder CSA farmers, of whom 376 (87.9%) were practicing agroforestry. Primary data was collected using interview guides developed through the Kobo Collect application. Microsoft Excel and Statistical Package for Social Sciences (SPSS) statistical packages were used to process and analyze the data. This study found that wood fuel was the main source of energy for 73% of the smallholder agroforestry farmers, followed by solar energy devices (12%), charcoal (7%), electricity (6%), and kerosene (2%). Grevillea Robusta was the most widely preferred agroforestry tree variety, adopted by 91.8% of agroforestry smallholder farmers, followed by fruit trees (73.4%), blue gums (67.3%), Cyprus (34.0%), and Calliandra (37.5%). Fodder tree types such as Sesbania and Casuarina were adopted by 30.9% and 23.9% of the smallholder agroforestry farmers, respectively. This study recommends the integration of agroforestry into farming systems by incentivizing farmers through programs like tree planting, free seedlings, and farm competitions. School agricultural clubs, such as 4K clubs for primary schools and Young Farmers Clubs for secondary schools, could be motivated and supported to use school gardens for food and fruit tree production. This study also recommends the promotion of modern stoves (maendeleo jikos), which use less wood fuel, are more efficient in cooking, and also reduce GHG emissions.

Favorite Parts of a Single Leaf for Giant Flying Squirrels to Eat in Three Species of Food Trees.

To examine the effect of leaf chemical composition on selective herbivory by the Japanese giant flying squirrels (Petaurista leucogenys), we measured and compared the total phenolic, glucose, and water contents of leaves among their main food tree species, deciduous Quercus acutissima, and evergreen Q. sessilifolia and Phonitia serratifolia. Leaves of these three tree species were available in the warm season (April to October), but the flying squirrels mostly preferred the leaves of Q. acutissima, having higher glucose and water contents than those of the other two tree species. In the cold season (November to the next March), the two evergreen tree species were available, and the flying squirrels used both leaves without any apparent influence of the chemical compositions. On the other hand, the favorite parts of a single leaf differed among the three tree species. Flying squirrels dropped the individual leaves after partial consumption. Their feeding marks on the dropped leaves were distinguished into four types: apical, basal, central, and marginal parts of consumption. The basal parts of consumption were most frequent in Q. acutissima leaves in which more water was contained at the basal part, and the central part consumption followed, which may be related to a lower phenolic content and more glucose and water at the leaf center than its margin. In contrast, the apically consumed leaves dominated in Q. sessilifolia, with relatively homogeneous leaf chemical distribution except for more water at the center. In P. serratifolia, leaves consumed at the center were frequent, but those with marginal consumption were also observed, which may be related to its specific chemical distribution with less phenolics and more glucose at the leaf margin. Thus, the chemical distributions within the single leaf differ among tree species, and the flying squirrel's selectivity of the tree species and the part of each leaf depends partly on the relative compositions of preferable glucose and water and unpreferable phenolics.

Dynamic cytological and transcriptomic analyses provide novel insights into the mechanisms of sex determination in Castanea henryi.

Castanea henryi is a monoecious woody food tree species whose yield and industrialization potential are limited by its low female-to-male flower ratio. Here, the male flowers on the male inflorescence of C. henryi were converted to female flowers by triple applications of exogenous cytokinin (CK) (N-(2-chloro-4-pyridyl)-N'-phenylurea, CPPU). To study the role of exogenous CK in flower sex determination, cytological and transcriptomic analyses were performed on samples from the five stages after CK treatment. Cytological analysis showed that stage 3 (nine days after the last CK treatment) was the critical stage in the differential development of the pistil primordium and stamen primordium. On this basis, one key module and two modules with significant positive correlations with stage 3 were identified by weighted gene co-expression network analysis (WGCNA), combined with transcriptome data. The CK and GA biosynthesis- and signaling-related genes, three transcription factor (TF) families, and 11 floral organ identity genes were identified in the related modules. In particular, the TFs WRKY47, ERF021, and MYB4, and floral organ identity genes AGL11/15, DEF, and SEP1 with large differences are considered to be critical regulators of sex determination in C. henryi. Based on these results, a genetic regulatory network for exogenous CK in the sex determination of flowers in C. henryi is proposed. This study contributes to the understanding of the role of CK in the sex regulation of flowers and provides new insights into the regulatory network of sex determination in C. henryi.