Food Nutrient Research Articles

Pollen-Food Allergy Syndrome: From Food Avoidance to Deciphering the Potential Cross-Reactivity between Pru p 3 and Ole e 7.

Cross-reactivity between nonspecific lipid transfer proteins could cause anaphylaxis, further influencing food avoidance and nutrient deficiencies. The one affecting olive pollen (Ole e 7) and peach (Pru p 3) may underlie a variety of pollen-food syndromes, though a deep molecular analysis is necessary. Three Ole e 7-monosensitised patients (MON_OLE), three Pru p 3-monosensitised patients (MON_PRU) and three bisensitised patients (BI) were selected. For epitope mapping, both digested proteins were incubated with patient sera, and the captured IgE-bound peptides were characterised by LC-MS. The analysis revealed two Ole e 7 epitopes and the three Pru p 3 epitopes previously described. Interestingly, the "KSALALVGNKV" Ole e 7 peptide was recognised by MON_OLE, BI and MON_PRU patients. Conversely, all patients recognised the "ISASTNCATVK" Pru p 3 peptide. Although complete sequence alignment between both proteins revealed 32.6% identity, local alignment considering seven residue fragments showed 50 and 57% identity when comparing "ISASTNCATVK" with Ole e 7 and "KSALALVGNKV" with Pru p 3. This study mapped sIgE-Ole e 7-binding epitopes, paving the way for more precise diagnostic tools. Assuming non-significant sequence similarity, structural homology and shared key residues may underlie the potential cross-reactivity between Ole e 7 and Pru p 3 nsLTPs.

Assessment of Nutritional Status among the Adolescents - A Case Study

In India, the prevalence of under nutrition among adolescents is most common. Assessments of nutritional status play significant role in formulating developmental strategies of a country. The objectives of the present study is to assess the impact of nutritional supplementation in terms of nutritional grading and health status among student of age group of 19 to 21 years by the survey at University College Mangalore, Karnataka State. The survey carried out among students having lunch under Mid-day meal scheme, college canteen and food from their home. Anthropometric measurements were used to assess nutritional status and BMI of each student were calculated. Among these students, getting lunch box from home are healthier than the student having lunch at college canteen and mid-day meal scheme. This may be due to, these students get more nutrient food prepared at their home. Therefore there should be nutrition education and awareness sessions for students to increase their knowledge and benefit regarding proper diet and its effect on their health.

Pumpkin and Pumpkin By-Products: A Comprehensive Overview of Phytochemicals, Extraction, Health Benefits, and Food Applications.

A versatile and popular Cucurbitaceous vegetable, pumpkin has recently gained much attention because of its variety of phytochemicals and health advantages. Pumpkins are a type of winter squash, traditionally with large, spherical, orange fruits and a highly nutrient food. Pumpkin by-products comprise various parts, such as seeds, peels, and pulp residues, with their bioactive composition and many potential benefits poorly explored by the food industry. Pumpkin and their by-products contain a wide range of phytochemicals, including carotenoids, polyphenols, tocopherols, vitamins, minerals, and dietary fibers. These compounds in pumpkin by-products exhibit antioxidant, anticancer, anti-inflammatory, anti-diabetic, and antimicrobial properties and could reduce the risk of chronic diseases. This comprehensive review aims to provide a detailed overview of the phytochemicals found in pumpkin and its by-products, along with their extraction methods, health benefits, and diverse food and industrial applications. This information can offer valuable insights for food scientists seeking to reevaluate pumpkin's potential as a functional ingredient. Reusing these by-products would support integrating a circular economy approach by boosting the market presence of valuable and sustainable products that improve health while lowering food waste.

Exploration of Protease Resources in the Gut of Omnivorous Gryllotalpa orientalis (Orthoptera: Gryllotalpidae).

An insect's gut microbiome is an essential "organ" in their life cycle, playing a crucial role by aiding food digestion and nutrient absorption. This study employed both culture-independent and culture-dependent methods to explore the protease resources present in the gut of the omnivorous insect Gryllotalpa orientalis. The findings revealed that the gut extract of G. orientalis contained a diverse array of proteases, including cysteine proteases, pepsin, serine proteases, and trypsin, as well as some unidentified proteases. Furthermore, the protease gene htpX, derived from gut bacterium Priestia megaterium DX-3, has been cloned and recombinantly expressed. The recombinant DX-3-htpX protease exhibited a 61.9-fold increase in fermentation level compared to the DX-3 protease. This protease was characterized as a neutral, heat-resistant metalloprotease with an M48 peptidase domain, and it was observed that the binding of Ca2+ to the recombinant protease resulted in the formation of the largest active pocket. This study provides technical support for further development and utilization of functional protein resources in insect gut.

Is Nutrient Quality of the Locally-Existing, EAT-Lancet-like Plant-Based Diet Better or Worse than the Average Diet in Taiwan? An Example of Local Translation.

The EAT-Lancet commission advocated a planetary health diet in 2019. Some have raised concerns about its nutrient adequacy. This study used data from recent Nutrition and Health Surveys in Taiwan-from 2017 to 2020 (n = 6538)-to assess food intake and nutrient adequacy among three red meat consumption levels (low/medium/high). The low red meat group, whose diet was similar to the EAT-Lancet reference, showed significantly higher/better levels of vitamins C and E, calcium, magnesium, sodium, dietary fiber, and the polyunsaturated to saturated fatty acids ratio. However, protein, B vitamins, phosphorus for females, and zinc were slightly compromised, but they were still near or above 100% of the Daily Reference Intakes (DRIs), except for zinc (74~75%). The intake levels of vitamin D, calcium, and dietary fiber in all three groups at times did not reach 70% of the DRIs, but this was more pronounced in the high red meat group compared to the low red meat group. Replacing ultra-processed foods (UPFs) with whole/healthy foods improved levels of zinc, calcium, and dietary fiber, but not vitamin D. Finally, a proposed local planetary health dietary construct was provided, suggesting maintaining the original distribution of the food groups recommended by the Taiwan Food Guide while specifying amounts of protein sources in line with the EAT-Lancet principles. The proposed diet, according to our estimation and comparison with Taiwanese DRIs, was nearly perfect in its nutrient composition.

Macroinvertebrates Associated with Macroalgae within Integrated Multi-Trophic Aquaculture (IMTA) in Earthen Ponds: Potential for Accessory Production

The present work aims to evaluate the macroinvertebrate community associated with macroalgae in earthen pond systems to better understand their potential in detritus recycling and as an accessory production. Sampling took place on the settling pond of an aquaculture research station, where macroalgae permanently occurred at high densities. The results suggest differentiation between seasons but not between sites within the settling pond. Seasonal variation was observable in terms of macroinvertebrate density, biomass, and diversity. Two non-indigenous species of invertebrates were found, the crustaceans Grandidierella japonica and Paracerceis sculpta Amphipods were the most abundant group, and their high nutritional value can be exploited. Detritus and the epiphyte layer are the main food items for the invertebrates, reinforcing the advantages of these organisms being present to enhance the recycling of excess detritus and to transfer organic matter to upper trophic levels. These species, naturally present in aquaculture facilities, can improve the water quality and increase the variability of food nutrients for reared species.

Endobariatric systems: Strategic integration of endoscopic therapies in the management of obesity.

The escalating obesity pandemic and its comorbidities necessitate adaptable and versatile treatment strategies. Endobariatric and metabolic therapies (EBMTs) can be strategically employed in a multipronged approach to obesity management, analogous to the way chess systems are employed to seize opportunities and thwart threats. In this review, we explore the spectrum of established and developing EBMTs, examining their efficacy in weight loss and metabolic improvement and their importance for a tailored, patient-centric approach. The complexity of obesity management mirrors the intricate nature of a chess game, with an array of tactics and strategies available to address the opponent's moves. Similarly, the bariatric endoscopist employs a range of EBMTs to alter the gastrointestinal tract landscape, targeting critical anatomical regions to modify physiological reactions to food consumption and nutrient assimilation. Gastric-focused EBMTs aim to reduce stomach capacity and induce satiety. Intestinal-focused EBMTs target hormonal regulation and nutrient absorption to improve metabolic profiles. EBMTs offer unique advantages of reversibility, adjustability and minimal invasiveness, allowing them to be used as primary treatments, adjuncts to pharmacotherapy or tools to address post-bariatric surgery weight recidivism. However, sub-optimal adoption of EBMTs due to lack of awareness, perceived costs and limited training opportunities hinders their integration into standard obesity management practices. By strategically integrating EBMTs into the broader landscape of obesity care, leveraging their unique advantages to enhance outcomes, clinicians can offer a more dynamic and personalized treatment paradigm. This approach, akin to employing chess systems to adapt to evolving challenges, allows for a comprehensive, patient-centric management of obesity as a chronic, complex and relapsing disease.

Mechanobiological factors in fermentation: Understanding the impact on food texture and nutrient release

Evaluating people’s food intake is crucial for establishing the link between diet and disease. Deprivation of vital nutrients causes the body to deteriorate organs and increases the risk of severe diseases that manifest in maturity. Making healthy food choices is one of the best ways to avoid developing chronic diseases, such as diabetes, heart disease, stroke, and even certain types of cancer. Hence, this paper proposes a deep learning-based automated nutrition classification system (DL-ANCS) for predicting food ingredients and nutrition. The DL-ANCS with Internet of Things (IoT) sensors to quantify food nutrition and a smartphone app to compile ingredient nutritional information. It is possible to take pictures of food eaten using the camera that comes with most mobile phones. It is possible to automatically identify the food items for record keeping using image processing. The effectiveness of the proposed DL-ANCS relies on its ability to accurately classify food items in these photos utilizing meal prediction algorithms and DL-ANCS. This research introduces a novel approach to extracting texture information from food photos to show how these features improve the accuracy of a nutritional assessment system that runs on mobile phones. The proposed method improvesthe food texture ratio of 98.7%, effectiveness ratio of 99.2%, accuracy ratio of 95.89%, food ingredient predictionsand their nutritional compatibility ratio of 96.8%, and food component classifications ratio of 97.29%.

Seasonal variation in the intake of food groups and nutrients in Japan: A systematic review and meta-analysis.

Background Seasonal variations could systematically bias dietary intakes. This systematic review aimed to determine seasonal variations in dietary intake among Japanese adults.Methods PubMed and Ichushi-Web databases were searched for studies reporting seasonal intakes of nutrients or food groups assessed by dietary records or 24-hour recalls. The protocol was registered with PROSPERO (CRD42022356084).Results Ten studies (eight studies on 1-31 nutrients and four on 2-15 food groups) met the inclusion criteria. Six studies included both sexes, whereas four investigated only females. The number of participants ranged from 25 to 459, and the number of dietary survey days in each season ranged from 1 to 14. For most nutrient and food groups, the reported seasonal variations were inconsistent across studies. The meta-analyses comparing differences in mean intakes between two seasons showed no significant differences in all comparisons or differences in only one comparison for most nutrients and food groups. Significant seasonal differences were observed for vegetables, fruits, and potatoes in five out of six comparisons, though the heterogeneity was high. Their biggest differences were as follows: 101 g/day more vegetable intake in summer than spring, 60 g/day more fruit intake in fall than spring, and 20.1 g/day more potato intake in fall than spring.Conclusion Reported seasonal variations were inconsistent across studies for most food groups and nutrients. Relatively distinct seasonal differences in mean intakes were observed for vegetables, fruits, and potatoes in meta-analyses. However, these results must be interpreted cautiously because of the high heterogeneity and limited representativeness.

Vegetarian Diets During Pregnancy: With Supplementation, Ovo-Vegetarian, Lacto-Vegetarian, Vegan, and Pescatarian Adaptations of US Department of Agriculture Food Patterns Can Be Nutritionally Adequate

BackgroundThe 2020-2025 Dietary Guidelines for Americans (DGA) includes a lacto-ovo vegetarian pattern (the healthy vegetarian dietary pattern [HVDP]) as a recommended dietary pattern during pregnancy. ObjectiveTo adapt the HVDP for vegan, ovo-vegetarian, lacto-vegetarian, and pescatarian diets during pregnancy. DesignUsing food pattern modeling, 4 adaptations of the HVDP were developed at energy levels that may be appropriate during pregnancy (1800, 2000, 2200, 2400, and 2600 kcal/day). Models were run both with and without the addition of a composite prenatal supplement. Main outcome measuresMain outcome measures were macro- and micronutrient adequacy without exceeding recommendations for saturated fat and added sugar. Statistical analysis performedThe 2020-2025 DGA Food Pattern Modeling Report was used to define food groups and nutrients in the HVDP. The HVDP was revised to remove dairy and/or eggs or to add seafood. ResultsAcross all examined energy levels (1800, 2000, 2200, 2400, and 2600 kcal per day), modeled dietary patterns provided sufficient macronutrients. Without prenatal supplements, each dietary pattern met most, but not all, micronutrient recommendations. Micronutrients that were below recommendations in patterns without supplements included vitamin D, iron, vitamin E, sodium, and choline. With the addition of a composite prenatal supplement to these patterns, the nutrients below 100% of recommendations were vitamin D, choline, and sodium. ConclusionsOverall, these results show that a HVDP and similar diets without meat, eggs, dairy, and/or seafood can provide most nutrients needed during pregnancy, albeit with some micronutrient challenges similar to those diets that include meat and other animal products.

Dietaryindex: a user-friendly and versatile R package for standardizing dietary pattern analysis in epidemiological and clinical studies

BackgroundFew standardized and open-source tools exist for calculating multiple dietary pattern indexes from dietary intake data in epidemiological and clinical studies. ObjectivesThe primary aim is to develop and validate dietaryindex, a user-friendly and versatile R package that standardizes the calculation of dietary indexes. MethodsDietaryindex can calculate multiple dietary indexes of high interest in research, including the Healthy Eating Index (HEI)-2020, the Alternative HEI 2010, the Dietary Approaches to Stop Hypertension Index, the Alternate Mediterranean Diet Score, the Dietary Inflammatory Index, the American Cancer Society 2020 dietary index, and the Planetary Health Diet Index from the EAT-Lancet Commission. The package includes generic dietary index calculation functions that accept any dietary assessment with preprocessed serving sizes of food groups and nutrients as defined by the research group that developed each index. For ease of use and to eliminate any need for data preprocessing, dietaryindex also offers 1-step functions that directly reference common datasets and tools, including the National Health and Nutrition Examination Survey (NHANES), the Automated Self-Administered 24-h Dietary Assessment Tool (ASA24), the Diet History Questionnaire III (DHQ3), and the Block Food Frequency Questionnaire. At least 2 independent researchers validated the serving size definitions and scoring algorithms of dietaryindex. ResultsIn our validation process, dietaryindex demonstrated full accuracy (100%) in all generic functions with 2-decimal rounding precision when compared with hand-calculated results. Similarly, using NHANES 2017–2018 data and ASA24 and DHQ3 example data, the HEI-2015 outputs from dietaryindex aligned (99.95%–100%) with results using the Statistical Analysis System codes from the National Cancer Institute. ConclusionsDietaryindex is a user-friendly, versatile, and validated informatics tool for standardized dietary index calculations. We have open-sourced all validation files and codes with detailed tutorials on GitHub (https://github.com/jamesjiadazhan/dietaryindex).

The Effect of Daily Avocado Intake on Food and Nutrient Displacement in a Free-Living Population with Abdominal Obesity

BackgroundAvocado intake has been associated with improvements in diet quality. Whether this response is because of avocado intake, per se, or combined with a food and/or nutrient displacement (D) has yet to be determined. ObjectivesThis secondary analysis, conducted using dietary data from the Habitual Diet and Avocado Trial, sought to assess the effect of consuming a large avocado (168 g, 281 kcal) daily in the avocado-supplemented diet (AD) group compared with the habitual diet (HD) group on food and nutrient D. MethodsUsing data from 3 unannounced telephone 24-h dietary recalls conducted during the 6-mo intervention period, food intake data were evaluated for 898 participants, aged 25–87 y, and a BMI range of 20.5–60.3 kg/m2. The food group distribution, energy, and nutrient intake of the AD group (n = 436) were calculated and compared with those of the HD group (n = 462). ResultsThe AD group had a higher daily intake of energy (159 ± 575 kcal) (P < 0.001), potassium (3193 ± 817 mg compared with 2419 ± 843 mg) (P < 0.005), fiber (30 ± 8 g compared with 19 ± 9 g) (P < 0.05), and a lower daily intake of animal protein (49 ± 33 g compared with 55 ± 24 g) (P = 0.02) compared with the HD group. Partial D with an avocado was observed for energy (43%), total fat (23%), and carbohydrate (87%), indicating a lower intake of these nutrients from non-avocado sources in the AD group. Food group analysis revealed a lower consumption of animal-derived protein from red meat, processed meats, poultry, and fish in the AD group, with no significant differences observed in dairy and egg intake between groups. ConclusionsIncorporating 1 avocado daily led to favorable modifications in the dietary composition of participants, including an increase in potassium and fiber intake, which can improve diet quality.This trial was registered at https://clinicaltrials.gov as NCT03528031.

Changes in food consumption and nutrition intake of rural residents in central China

Food security and diet diversity is essential to sustainable food system all over the world. As the income of rural residents achieves great increase, the structure of food consumption and food diversification face significant change. Rural residents' food consumption and nutrients intake worth more attention. Chinese government has been striving to achieve sustainable development in rural areas. We conducted this study to explore area-level practices aiming at achieving food and nutrition security in rural China. In order to search for the change principle and main influencing factors of residents' food consumption, three rural areas in Henan Province were selected. According to the data obtained from the Henan Province Bureau of Statistics, changes in food consumption from 2012 to 2021 in three rural areas were analyzed in this study. This study led to the following remarkable results: (1) The per capita consumption of poultry, meat, sugar, and eggs of the three rural residents has increased much more than that of other food items. (2) In the three rural areas, the proportions of grains, vegetables, liquor, and edible oils have decreased overall. The proportions of other categories, such as poultry, meat, and fruits, have increased. (3) The rural residents’ per capita nutrition intake has increased remarkably. The results provide some empirical foundation for local government who need suggest that rural residents should control their intake of high-energy food, such as poultry, meat, and sugar. This study has significant policy implication for achieving sustainable goals in rural areas of China.

Enzymatic acylation of cyanidin-3-O-glucoside with aromatic and aliphatic acid methyl ester: Structure–stability relationships of acylated derivatives

Anthocyanins are water-soluble pigments, but they tend to be unstable in aqueous solutions. Modification of their molecular structure offers a viable approach to alter their intrinsic properties and enhance stability. Aromatic and aliphatic acid methyl esters were used as acyl donors in the enzymatic acylation of cyanidin-3-O-glucoside (C3G), and their analysis was conducted using ultraperformance liquid chromatography–mass spectrometry (UPLC-MS). The highest conversion rate achieved was 96.41 % for cyanidin-3-O-(6″-feruloyl) glucoside. Comparative evaluations of stability revealed that aromatic acyl group–conjugated C3G exhibited superior stability enhancement compared with aliphatic acyl group derivatives. The stability of aliphatic C3G decreased with increasing carbon chain length. The molecular geometries of different anthocyanins were optimized, and energy level calculations using density functional theory (DFT) identified their sites with antioxidant activities. Computational calculations aligned with the in vitro antioxidant assay results. This study provided theoretical support for stabilizing anthocyanins and broadened the application of acylated anthocyanins as food colorants and nutrient supplements.

DYNAMICS OF A TRI-TROPHIC LEVEL MODEL WITH EXCESS FOOD NUTRIENT CONTENT AND INTRAGUILD PREDATION STRUCTURE

The dynamics of consumers can be affected by both nutrient-deficient food (low phosphorus (P) to carbon (C) ratio) and nutrient-excessive (high P:C) food, which is known as the stoichiometric knife-edge phenomenon. In this study, aiming to illustrate the responses of herbivores and omnivores to food with excessive P content, we present and analyze a tri-trophic level stoichiometric knife-edge model incorporating the intraguild predation structure. The model dynamics are theoretically examined, including the boundedness and positivity of solutions, the existence and stability of boundary equilibria, and the existence of positive equilibria. Numerical simulations show that the model has complex dynamics, encompassing chaotic-like oscillations, multiple types of bifurcations, long transients, and regime shifts. Moderate P levels facilitate the coexistence of the three species, whereas both lower and higher P levels result in the extinction of herbivores and omnivores. Specifically, the energy enrichment paradox occurs at low P levels. In contrast, the nutrient enrichment paradox occurs at high P levels, both of which are detrimental to the survival of herbivores and omnivores. Furthermore, the high-intensity predation of producers by omnivores helps to restrain the occurrence of chaotic dynamics. These findings contribute to a deeper understanding of the mechanisms of multi-species coexistence.

Study on the impact of adopting organic practices on the absorption and extractability of Cu and Zn in commercial coffee samples

Although coffee is not a primary source of minerals in the diet, due to its frequent consumption, it can be considered an important source of some micronutrients, highlighting those with antioxidant properties such as Cu and Zn. The availability of nutrients in foods consumed through infusion is related to solubility, which is influenced by the metabolization of these elements by the plant. Several factors can impact this metabolism, including cultivation. This study investigated the influence of cultivation on the absorption and extractability of Cu and Zn in conventional and organic commercial coffee. It was observed that cultivation influences the absorption of the minerals studied in opposite ways: while organic samples have higher levels of Zn, conventional samples have higher levels of Cu. Cultivation does not influence the extractability of Zn in coffee infusions, therefore indicating little influence on the metabolization of this element. Copper metabolism, on the other hand, is influenced by cultivation, with Cu exhibiting greater affinity and lower extractability in organic coffee samples compared to conventional ones.

Regulated anthocyanin release through novel pH-responsive peptide hydrogels in simulated digestive environment

The instability of anthocyanins significantly reduces their bioavailability as food nutrients. This proof-of-concept study aimed to develop efficient carriers for anthocyanins to overcome this challenge. Characterization of the hydrogels via SEM (scanning electron microscope) and rheological analysis revealed the formation of typical gel structures. MTT (methyl thiazolyl tetrazolium) and hemolysis assays confirmed that their high biocompatibility. Encapsulation efficiency analysis and fluorescence microscopy images demonstrated successful and efficient encapsulation of anthocyanins by pH-responsive hydrogels. Stability studies further validated the effect of peptide hydrogels in helping anthocyanin molecules withstand factors such as gastric acid, high temperatures, and heavy metals. Subsequently, responsive studies in simulated gastric (intestinal) fluid demonstrated that the pH-responsive peptide hydrogels could protect anthocyanin molecules from gastric acid while achieving rapid and complete release in intestinal fluid environments. These results indicate that these peptide hydrogels could stabilize anthocyanins and facilitate their controlled release, potentially leading to personalized delivery systems.

Putting the Asymmetric Response Concept to the test: Modeling multiple stressor exposure and release in a stream food web

Communities in stream ecosystems often respond asymmetrically to increase and release of stressors, as indicated by slow and incomplete recovery. The Asymmetric Response Concept (ARC) posits that this is due to a shift in the relative importance of three mechanisms: tolerance, dispersal, and biotic interactions. In complex natural communities, these mechanisms may produce alternative outcomes through poorly understood indirect effects. To understand how the three mechanisms respond to different temporal stressor scenarios, we studied multiple scenarios using a stream food web model. We asked the following questions: Do groups of species decline as expected on the basis of individual tolerance rankings derived from laboratory experiments when they are embedded in a complex dynamic food web? Does the response of ecosystem function match that of communities? To address these questions, we aggregated data on individual tolerances at the level of functional groups and studied how single and multiple stressors affect food web dynamics and nutrient cycling. Multiple stressor scenarios involved different intensities of salt and temperature increase. Functional groups exhibited a different relative tolerance ranking between the laboratory and dynamic food web contexts. Salt as a single stressor had only minor and transient effects at low level but led to the loss of one or more functional groups at high level. In contrast, high temperature, alone or in combination with salt, caused the loss of functional groups at all tested levels. Patterns often differed between the response of communities and ecosystem function. We discuss our findings with respect to the ARC.

Inadequacy of nutrients in children’s diets across seasons along an agricultural intensification gradient in Ethiopia

Although the production-to-consumption pathway is widely promoted to improve diet quality in developing world, its contribution to individual diet and nutrient intake remains unclear. We assessed this relationship among 377 children aged 6 to 59 months in three zones characterized by landscape diversity along an agricultural intensification gradient from the state forest of Munesa to the nearby town of Arsi Negele, Ethiopia during the two harvest seasons. A repeated interactive multiple-pass 24-h recall method was used to collect intake data. Usual intake distributions for energy, protein, iron, zinc and vitamin A were estimated using the National Cancer Institute (NCI) method and compared with estimated average requirement values to determine the prevalence of inadequate intake. The usual intake of protein, zinc and vitamin A among children were inadequate and further exacerbated by seasonality. The extent of nutrient inadequacy was higher in the diverse landscape (“near to forest” zone) than in the less diverse landscape (“distant to forest” zone). However, the diverse landscape tended to provide a better buffering capacity against seasonal energy and nutrient stress than a less diverse landscape. The age of a child positively predicted usual intakes of energy, protein, iron, zinc and vitamin A along the gradient, while breastfeeding negatively predicted these intakes. Most of the food groups and nutrients consumed were derived from on-farm production, whereas fruits and vegetables were via the market. Thus, introduction of nutrient-dense crops and increasing livestock productivity are vital to enhance consumption of diverse diets and thereby nutrients among children.

Assessment of Nutritional Status and Energy Availability of Indian Boxers.

Inadequate dietary intake and low energy availability (LEA) negatively impact athlete's health, recovery, and performance outcomes. Currently, there is no Indian study that assessed the energy availability (EA) of Indian boxers and studied the contributing factors to LEA. This study was undertaken with the objectives of assessing the EA of Indian boxers (lightweight category) and correlating it with contributing factors such as anthropometric measurements, body composition, dietary intake, nutrient intake, and exercise energy expenditure. This descriptive cross-sectional study recruited 30 state-level boxers of the lightweight category (females: nine) residing in Mumbai, Maharashtra, India, using purposive sampling. Body composition was assessed using a body composition monitor. Anthropometric measurements were assessed using standard methods. Three-day exercise energy expenditure was calculated. Three-day dietary intake was assessed, which included two training days and one rest day using the three-day multiple-pass method. Dietary intake assessment included intake of all the meals, snacks, sports foods, sports, and dietary supplements. EA was calculated and participants were classified as moderate EA (30-45 kcal/kg FFM/day) and low EA (< 30 kcal/kg FFM/day). Descriptive and inferential statistical tests were conducted using Statistical Product and Service Solutions (SPSS, version 23; IBM SPSS Statistics for Windows, Armonk, NY). Male boxers had a mean age of 18.43±0.93 years, and females had a mean age of 18.44±0.88 years. Among boxers, 36.67% (n=11) of participants were underweight, 46.67% (n=14) had a normal BMI, and 16.67% (n=5) were overweight. The mean body fat (%) for boxers was 13.11±1.8. Participants had insufficient consumption of different food groups before and after the energy intake adjustment. Processed packaged food consumption was popular among the participants. Various kinds of sports foods and supplements were consumed by a few athletes. Dietary supplements were not common among participants. The nutrient intake of the participants was inadequate. Participants could not fulfill energy/kg/day, carbohydrate/kg/day, protein/kg/day, and fat/kg/day requirements by 29%, 36.2%, 34.8%, and 23.9%, respectively. Iron and calcium intake was inadequate among female participants by 1.4% and 12.8%, respectively. Among male boxers, the mean EA was 29.71±3.74 kcal/kg FFM/day, and the mean EA of female boxers was 28.3±3.95 kcal/kg FFM/day. Around 66.67% of boxers in the present study had LEA, while the rest had reduced EA. EA had a moderately significant inverse relationship with fat-free mass (%). Energy intake was significantly related to EA and total body muscle % significantly moderated that relationship. The influence of fat-free mass (%), EEE (kcal), and energy intake (kcal)on the EA (kcal/kg FFM/day) was significant usingmultiplelinear regression analysis. The majority of the participants could not meet the food intake and nutrient recommendations. The majority of the participants had LEA. The higher the energy and nutrient intake, the higher the EA in the present study. EA had a moderately significant inverse relationship with fat-free mass (%) and exercise energy expenditure.