Food In China Research Articles

Effect of Ultra-High-Pressure Treatment on Gastrodia elata Blume: Drying Characteristics, Components, and Neuroprotective Activity

Gastrodiae Rhizoma (GE), a popular food in China, is stored and consumed after steaming, which can lead to the degradation of active substances and a decrease in its quality. Therefore, this study explored the potential application of ultra-high-pressure (UHP)-assisted hot air drying in improving the quality of GE. The results indicated that UHP pre-treatment could preserve the original cross-sectional color of GE and increase the degrees of freedom of water in GE samples. Compared with traditional steaming pre-treatment (18 h), UHP pre-treatment at 500 MPa significantly shorted the time (10 h) required for the GE samples to reach drying equilibrium. Meanwhile, the UHP-assisted hot air drying method (60 °C) could reduce the activity of β-D-glucosidase and avoid the degradation of active substances. Finally, UHP pre-treatment improved the neuroprotective activity in vivo. Overall, UHP-assisted hot air drying could improve the quality of GE samples. This study provides a simple method for improving the quality of GE samples and offers a reference for subsequent research on the influence of UHP on GE.

Analysis of Different Strains Fermented Douchi by GC×GC-TOFMS and UPLC-Q-TOFMS Omics Analysis.

Douchi is a kind of soybean-fermented food in China. To explore the common and differential compounds in different Douchi, Douchi was fermented by Aspergillus niger, Rhizopus arrhizus, and Bacillus circulans, respectively, and co-fermented by the three strains in this study. The common and characteristic flavor compounds and common and characteristic non-volatile components of different strains of fermented Douchi were explored through GC×GC-TOFMS and UPLC-Q-TOFMS omics analysis. The result suggested that Pyrazines, ketones, and alkenes such as tetramethyl-pyrazine, 2,5-dimethyl pyrazine, furaneol, 2,3-butanedione, gamma-terpinene might contribute to the basic flavor of the Douchi fermented by A. niger, R. arrhizus, and B. circulans. Peptides, amines, and flavonoids, such as N-acetylhistamine, 7,3',4'-trihydroxyflavone, (3S,8As)-3-isobutylhexahydropyrrolo[1,2-a]pyrazine-1,4-dione might contribute to the basic function of the above three Douchi. The common metabolic pathways involved in the fermentation were isoflavonoid biosynthesis, flavonoid biosynthesis, etc. Ketones and esters such as 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one, 3-octanone, 5-methylfurfural and nonanal contributed to the unique flavor, while betaine, oleanolic acid, saikosaponin D and leucine might contribute to the unique function of A. niger fermented Douchi. Alkenes, pyrazine, and ketones such as α-terpinene, ethyl-pyrazine, dihydro-3-methyl-2(3H)-furanone, and linalool might contribute to unique flavor, while cordycepin, 2-Phenylacetamide might contributed to the unique function of R. arrhizus fermented Douchi. The unique flavor of B. circulans fermented Douchi might derived from ketones and esters such as 3-acetyl-2-butanone, 2-tridecanone, propionic acid-2-phenylethyl ester, while vitexin, astragalin, and phenethylamine might contribute to the unique function. Compared with single-strain fermented Douchi, the flavor substances and non-volatile components in multi-strain fermented Douchi were more abundant, such as hexadecanoic acid methyl ester, benzeneacetic acid ethyl ester, 9,12-octadecadienoic acid ethyl ester, nuciferine, and erucamide. It was speculated that there were common and differential substances in Douchi fermented by Aspergillus niger, Rhizopus arrhizus, and Bacillus circulans, which might contribute to the basic and unique flavor and function. Compared with single-strain fermented Douchi, the flavor substances and metabolites in multi-strain fermented Douchi were more abundant. This study provided a reference for the research of flavor and functional substances of Douchi.

Evolutionary decision‐making behaviour among the government, leading enterprises and farmers: Based on the improvement of phaeozem quality in Northeast China

AbstractThe improvement of phaeozem quality in Northeast China is crucial to China's food security. This study incorporated government, leading enterprises and farmers into an evolutionary game model to explore the ideal state among the government, leading enterprises and farmers with the improvement of the phaeozem quality in Northeast China, thereby contributing to food security. The study includes the government, leading enterprises and farmers in an evolutionary game model to explore game relationship and evolution paths of decision‐making among the three stakeholders, and obtains the conditions for the three parties to evolve to the ideal state by analysing and combining with the actual data. The evolutionary process of the decision‐making behaviour of the three stakeholders to an ideal state is affected by many factors, and the value ranges of the different factors have different impacts on the convergence speed of the three stakeholders to an ideal state; when certain conditions are met, the decision‐making behaviours of the three stakeholders can evolve to an ideal state, which aims to provide beneficial suggestions for the government to supervise the quality of phaeozem, leading enterprises to control green agricultural products and farmers to apply organic fertilizer.

Impact of urbanization and planting structure on water footprint of food, cash, and feed crops in China’s northern agro-pastoral ecotone

China, especially the northern agro-pastoral ecotone, is facing severe water scarcity, which presents a significant challenge for the agriculture sector, the largest consumer of water. This study selected Yanchi County as a typical area. First, the water footprint method is used to evaluate the water usage of food, cash, and feed crops in the region from 1990 to 2020. Then, the vector error correction model (VECM) is used to analyze the short-term effects and long-term effects of urbanization and planting structure on the water consumption of these three types of crops. The results show: 1) The total agricultural water footprint (WF) in Yanchi County, as well as the WFs of the predominantly grown food and feed crops, show an upward trend. Additionally, the proportion of blue WF is relatively high across all crop types. 2) Reducing the proportion of food crops will increase the WF of cash and feed crops, but it will not necessarily decrease the WF of food crops. 3) An increase in the level of urbanization may reduce the agricultural WF in the short term, but ultimately, it will lead to an increase in the WF. Overall, this study reveals the growing pressure on agricultural water use faced by the agro-pastoral ecotone during the processes of urbanization and planting structure transformation. It highlights the importance of developing rain-fed agriculture, seizing the technological development opportunities brought by urbanization, and reasonably adjusting the cropping structure for the sustainable use of water resources.

Multi-frequency power ultrasound (MFPU) pretreatment of crayfish (Procambarus clarkii): Effect on the enzymatic hydrolysis process and subsequent Maillard reaction

Crayfish, as a new consumer trend, has become one of the most popular freshwater aquatic foods in China, and its processed product output has been increasing year by year. In this study, the effect of multi-frequency power ultrasound (MFPU) pretreatment in various working modes including mono-, dual- and tri-frequency on the enzymatic hydrolysis and Maillard reaction of crayfish was investigated. Additionally, the underlying mechanism was also explored. Results showed that the degree of hydrolysis (DH) of crayfish was 23.89 %, while it increased to 40.78 % after MFPU pretreatment at 20/40 kHz dual-frequency, indicating that MFPU pretreatment significantly (p < 0.05) enhanced the enzymatic hydrolysis of crayfish. The crayfish protein after MFPU pretreatment exhibited a decrease in α-helix and increase in β-fold content, and the occurrence in the unfolding of the protein structure and alteration of tertiary structure. According to scanning electron microscopy (SEM) and atomic force microscopy (AFM) analyses, the MFPU pretreatment resulted in the fragmentation of crayfish protein. During the analyses of subsequent Maillard reaction, the composition and quantity of volatiles detected by the electronic nose and GC–MS indicated that MFPU pretreatment enhanced flavor of the Maillard reaction products. In conclusion, MFPU pretreatment is a promising technology for improving the degree of hydrolysis of crayfish protein and enhancing the flavor of Maillard reaction products.

Distribution of polycyclic aromatic hydrocarbons and carcinogenic risk assessment in street-barbecued foods in China

Street-barbecued foods, despite their popularity, produce carcinogenic polycyclic aromatic hydrocarbons (PAHs) such as benzo[a]anthracene (BaA), chrysene (Chr), benzo[b]fluoranthene (BbF), benzo[a]pyrene (BaP) due to incomplete charcoal combustion, direct food-fuel contact, and excessive heating. This study analyzed barbecued foods from high-traffic street vendors using high-performance liquid chromatography. Among five food categories (meat, vegetable, aquatic, starch, and soy products), eight foods exceeded China’s BaP limit (5 µg/kg) and twelve exceeded the EU limit (2 µg/kg). The highest BaP and PAH4 levels were found in barbecued beef and pork belly, respectively. Chr levels were notably high due to open flame exposure. Health risk assessments indicated fourteen foods posed elevated carcinogenic risks. This study highlights the necessity of consumption control to mitigate these risks and provides evidence for improving street food safety and dietary security.

Total flavonoids from Rosa rugosa Thunb.: A comprehensive review of its extraction and purification process, chemical composition, biological effect and applications.

Rosa rugosa Thunb. is a kind of traditional Chinese medicine with homology of medicine and food in China for a long history. The total flavonoids from R. rugosa Thunb. (TFR), possessing antioxidant, anti-tumor, antidepressant, and antibacterial activities, can effectively prevent and treat cardiovascular, hyperglycemia, hyperlipidemia, and other diseases. However, few studies have focused on TFR analyses and no comprehensive review has yet been published. In order to provide a theoretical foundation and research direction for further study and development, this paper methodically reviewed the extraction and purification procedure, chemical composition, biological activity, toxicology, and application in food of TFR on the basis of previous research. A total of 102 distinct flavonoids have been isolated from R. rugosa Thunb. Further pharmacological activities and chemical analyses research will contribute to the development of TFR products in R. rugosa Thunb.

Exploring Foreign Tourists Intentions toward Novel Food in China: The Impact of Food Neophobia as a Key Moderator

Exploring Foreign Tourists Intentions toward Novel Food in China: The Impact of Food Neophobia as a Key Moderator

More ESG Practices, Higher Financial Performance? The U‐Shaped Pattern in China's Agricultural and Food Firms

ABSTRACTESG (environmental, social, and governance) practices, an internationally recognized concept of a firm's sustainability, can serve as a bridge to enhance effective communication between firms and various stakeholders. It is also a new tool for measuring firms' value. This study employs a balanced panel data set of 114 publicly traded agricultural and food firms in China from 2016 to 2022. It empirically investigates the relationship between ESG and financial performance based on shareholder primacy theory and stakeholder theory. Employing a two‐way fixed effects (FE) model, our findings reveal a U‐shaped nonlinear pattern in how ESG impacts financial performance. It differs from previous studies that found predominantly positive relationships: Specifically, the average ESG score for firms is 0.396, the median is 0.390, and the inflection point of the U‐shape occurs at 0.53. When firms' ESG investments are less than 0.53, the high initial ESG cost makes it difficult to transform ESG into financial improvement. However, once this threshold is surpassed, good ESG can convey an excellent reputation to the public and increase anti‐risk ability—which, in turn, significantly improves firms' financial performance and facilitates long‐term development. Also, our study demonstrates that firms' ownership and different positions in the supply chain differentially affect the relationship between ESG and financial performance. This paper also offers insights into promoting the sustainable development of Chinese agri‐food firms.

Bioactivity-Oriented Separation of "Pepper Alkaloids" from Piper sintenense Hatusima with Potential Antigouty Arthritis Activity.

Piper sintenense Hatusima (PsH) is a member of the Piper genus used as food and folk medicine in China. However, the detailed chemical ingredients and potential pharmacological effects are still underexploited. In this study, we evaluated the anti-inflammatory and antigouty arthritis effect of the ethanolic extract of P. sintenense. Active compounds were isolated from the petroleum ether fraction resulting in six novel amide alkaloids (1-6) and 13 known analogues (7-19). All of the compounds exhibited excellent anti-inflammatory activities. Bioinformatics analysis and in vitro experiments showed that compound 4 had anti-inflammatory properties (IC50 = 4.86 ± 0.32 μM) and antigouty arthritis activity. Mechanistic studies revealed that compound 4 exerted its favorable efficacy by regulating macrophage polarization by inhibiting P-STAT1/4. These findings provide new insights into the chemical composition and function of P. sintenense and expand its promising application as a functional food in the prevention and treatment of gouty arthritis.

An endophenotype network strategy uncovers YangXue QingNao Wan suppresses Aβ deposition, improves mitochondrial dysfunction and glucose metabolism

BackgroundAlzheimer's disease (AD), an escalating global health issue, lacks effective treatments due to its complex pathogenesis. YangXue QingNao Wan (YXQNW) is a China Food and Drug Administration (CFDA)- approved TCM formula that has been repurposed in clinical Phase II for the treatment of AD. Identifying YXQNW's active ingredients and their mechanisms is crucial for developing effective AD treatments. PurposeThis study aims to elucidate the anti-AD effects of YXQNW and to explore its potential therapeutic mechanisms employing an endophenotype network strategy. MethodsHerein we present an endophenotype network strategy that combines active ingredient identification in rat serum, network proximity prediction, metabolomics, and in vivo experimental validation in two animal models. Specially, utilizing UPLC-Q-TOF-MS/MS, active ingredients are identified in YXQNW to build a drug-target network. We applied network proximity to identify potential AD pathological mechanisms of YXQNW via integration of drug-target network, AD endophenotype gene sets, and human protein interactome, and validated related mechanisms in two animal models. In a d-galactose-induced senescent rat model, YXQNW was administered at varying doses for cognitive and neuronal assessments through behavioral tests, Nissl staining, and transmission electron microscopy (TEM). Metabolomic analysis with LC-MS revealed YXQNW's influence on brain metabolites, suggesting therapeutic pathways. Levels of key proteins and biochemicals were measured by WB and ELISA, providing insights into YXQNW's neuroprotective mechanisms. In addition, 5×FAD model mice were used and administered YXQNW by gavage for 14 days at two doses. Amyloid-β levels, transporter expression, and cerebral blood flow have been detected by MRI and biochemical assays. ResultsThe network proximity analysis showed that the effect of YXQNW on AD was highly correlated with amyloid β, synaptic function, glucose metabolism and mitochondrial function. The results of metabolomics combined with in vivo experimental validation suggest that YXQNW has the potential to ameliorate glucose transport abnormalities in the brain by upregulating the expression of GLUT1 and GLUT3, while further enhancing glucose metabolism through increased O-GlcNAcylation and mitigating mitochondrial dysfunction via the AMPK/Sirt1 pathway, thereby improving d-galactose-induced cognitive deficits in rats. Additionally, YXQNW treatment significantly decreased Aβ1–42 levels and enhanced cerebral blood flow (CBF) in the hippocampus of 5×FAD mice. while mechanistic findings indicated that YXQNW treatment increased the expression of ABCB1, an Aβ transporter, in 5×FAD model mice to promote the clearance of Aβ from the brain and alleviate AD-like symptoms. ConclusionsThis study reveals that YXQNW may mitigate AD by inhibiting Aβ deposition and ameliorating mitochondrial dysfunction and glucose metabolism, thus offering a promising therapeutic approach for AD.

Nutrient content and ultra-performance liquid chromatography tandem mass spectrometry metabolomic comparative analysis of Lithocarpus corneus and Lithocarpus pachylepis.

China possesses abundant species resources in genus Lithocarpus Blume, commonly known as "acorns." These nuts are traditional foods in China, holding significant potential for local specialty food and rural economic development. To explore their edible value, we compared the nutritional components and metabolites between the fruits of Lithocarpus corneus (YD) and Lithocarpus pachylepis (HL), which are cultivated widely in south of China. Non-targeted metabolomics analysis of YD and HL was performed using ultra-performance liquid chromatography tandem mass spectrometry technology. A total of eight classes of differential metabolites (DAMs) were detected: alkaloids, amino acids and peptides, carbohydrates, fatty acids, polyketides, shikimates and phenylpropanoids, terpenoids, and other metabolites. In the positive ion mode, 225 DAMs were identified, whereas in the negative ion mode, 116 DAMs were identified. KEGG pathway enrichment analysis showed that the differentially abundant metabolites were generally enriched in three pathways: aminoacyl-tRNA biosynthesis, alanine, aspartate, and glutamate metabolism, and ABC transporters. The nutritional content of YD has higher moisture, fructose, and total flavonoid levels, but lower starch, protein, fat, glucose, sucrose, and total sugar content than HL. Additionally, YD contains more Ca, K, P, and Fe, but less Mg and Zn compared to HL. Analyses indicate that YD is nutrient-rich and beneficial, making it suitable for further development. Two species of Lithocarpus contain nandrolone, suggesting a new direction for specialty food development. The unique chemical composition and rich nutritional content of Lithocarpus fruits provide a theoretical basis for their development and application as food resources. PRACTICAL APPLICATION: L. corneus and L. pachylepis are traditional Chinese nuts, rich in starch, minerals, and flavonoids, making them suitable for consumption or use as food constituents. Their fruits contain various unique natural chemical compounds, indicating high application value and development potential.

Evaluation of the Ecosystem Services Value of Agricultural Industrial Parks under the Background of Tianfu Granary Construction - A Case Study of the Daoming and Longxing Districts in Chongzhou City

In the new era, China faces two major challenges of food security and ecological protection. The agricultural industrial parks constructed in the process of developing the Tianfu Granary play a significant role in China's food supply and ecological security as a special type of farmland ecosystem. Accurately calculating the value of ecosystem services in agricultural industrial parks is of great importance for achieving sustainable agricultural development and comprehensively realizing rural revitalization. This paper takes the Daoming and Longxing Agricultural Industrial Parks in Chongzhou City as the research subjects, constructs an evaluation index system for ecosystem service value, and assesses and calculates the supply, support, regulation, and cultural services of the agricultural industrial parks. The research findings show that the total value of ecosystem services reaches 260.64 million yuan, which is 4.65 times the value of the agricultural products supplied, and its significant ecosystem service value cannot be ignored. Among the ecosystem service values of the park, the cultural service value is the highest, followed by the social security service value. Based on the above conclusions, this paper discusses how to achieve high-standard construction of agricultural industrial parks, providing a scientific basis for the government and policymakers to make correct decisions in the rational use of resources, reduce and avoid short-term economic behaviors that damage ecosystem service functions, and promote sustainable agricultural development.

Polyphenol composition and antioxidant activity of fermentation combined with enzymatic hydrolysis modified Astragalus membranaceus stems

Astragalus membranaceus (AM) roots are a well-known homologous medicine and food in China. AM stems, which are discarded and not used effectively, also contain many active compounds and exhibit beneficial effects. It has the potential to be explored as antibiotic alternative. Fermentation combined with enzymatic hydrolysis (FEH) is an effective strategy for extracting polyphenol and improving the usage of AM stems. Therefore, in this study, the conditions of FEH and extraction for polyphenol in AM stems were screened. The antioxidant activity of extract from AM stems without and with FEH (AMSE and FAMSE) was evaluated. The metabolite profiles of phenolic acids and flavonoids in AMSE and FAMSE were characterized by ultra-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (UPLC–ESI-MS/MS). The results showed that the highest polyphenol content from AM stems was obtained with cellulase and pectinase (1:1, 2000 U/g), moisture content 43%, fermentation temperature 30 °C, and fermentation time 7 days. Selected extraction conditions of polyphenol were ethanol concentration 50%, ultrasonic power 500 W, extraction temperature 35 °C, and extraction time 40 min. On this condition, compared with AMSE, the polyphenol and flavonoid contents in FAMSE were significantly higher. FAMSE exhibited stronger DPPH, hydroxyl radical scavenging rate and reducing power than AMSE. The relative content of 3-(4-hydroxyphenyl)-propionic acid, dihydroferulic acid, isoferulic acid, 4-hydroxybenzoic acid, 4-hydroxyphenyllactic acid, ferulic acid, vanillic acid, syringic acid, gentisic acid, sinapic acid, apigenin, tricin, acacetin, daidzein, genistein, formononetin, prunetin, pratensein, rhamnocitrin and galangin were significantly upregulated in FAMSE.Graphical

Coupling Relationships and Driving Mechanisms of Water–Energy–Food in China from the Perspective of Supply and Demand Security

The rapid increase in population and economy, coupled with accelerated urbanization, is placing immense pressure on the water–energy–food (WEF) system. In this context, the water–energy–food nexus framework has emerged, recognizing the interdependencies and interactions among water, energy, and food systems, with the aim of optimizing resource management through cross-sectoral collaboration to promote sustainable development. Understanding the spatio-temporal differentiation patterns of the WEF nexus and elucidating the driving mechanisms behind changes in their coupling relationships is essential. This knowledge is crucial for ensuring the security of each subsystem and enhancing the overall sustainability of interconnected systems through coordinated efforts. To address these challenges, this study first established evaluation indicators for water, energy, and food security to quantify their levels and spatio-temporal dynamics. Subsequently, the degrees of coupling coordination within the WEF nexus were calculated. Finally, the WEF nexus’s spatial correlations were analyzed by using a spatial autocorrelation model. Spatial econometric models then identified key factors affecting its coordination. The results revealed significant spatial heterogeneity in water, energy, and food security across mainland China’s provinces. From 2002 to 2022, water security improved substantially in 87% of the provinces, while energy security began to improve in the eastern regions following a phase of high consumption. Food security saw significant enhancements, particularly in Inner Mongolia and the northeastern provinces. The overall coupling coordination of the WEF nexus improved across 30 provinces, progressing toward primary coordination. However, Henan and Anhui provinces experienced fluctuations in WEF nexus coordination. Spatial correlation analysis showed upward trends and increased clustering in WEF nexus coordination. Factors such as economic development and population positively influenced coordination, while economic agglomeration, education, and effective irrigation area had negative effects. This study elucidates the complex interconnections and key influencing factors within the WEF nexus, providing a reference framework and practical recommendations for equitable resource management.

Platform Business Groups and the Omni‐Channel Transformation of Food Retailing in China

AbstractIn the past decade, China's food retail market has undergone significant restructuring driven by platform firms, enhancing omni‐channel capabilities across the sector, and bolstering the resilience of domestic retailers. These shifts have contributed to the exit of numerous international food retailers. Despite this transformation, there remains a lack of understanding regarding the economic geography of China's food retail market, especially in terms of digital platform operations. This paper aims to conceptualise two major Platform Business Groups (PBGs), Alibaba Group and Tencent‐JD.com Alliance, and investigate their respective roles in the omni‐channel transformation of Chinese food retailing. Drawing on forty semi‐structured interviews and diverse quantitative and qualitative sources, the study conceptualises two distinct PBG models shaping the market: Alibaba's ‘Integrated PBG’ and the Tencent‐JD.com Alliance's ‘Cooperative PBG’. The research explores how the two groups utilise online analytics directly through foodstore subsidiaries and indirectly by providing omni‐channel digital services to third‐party food retailers.

Chebulinic acid isolated from aqueous extracts of Terminalia chebula Retz inhibits Helicobacter pylori infection by potential binding to Cag A protein and regulating adhesion.

Terminalia chebula Retz, known as the King of Tibet, is considered a functional food in China, celebrated for its antioxidant, immune-modulating, antibacterial, and anti-inflammatory properties. Chebulinic acid, derived from aqueous extracts of Terminalia chebula Retz, is known for its anti-inflammatory properties. However, its potential as an anti-Helicobacter pylori (HP) agent has not been fully explored. Herein, we extracted the main compound from Terminalia chebula Retz using a semi-preparative liquid chromatography (LC) system and identified compound 5 as chebulinic acid through Ultra-high performance liquid chromatography-MS/MS (UPLC-MS/MS) and Nuclear Magnetic Resonance (NMR). To evaluate its role, we conducted minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) assays, scanning electron microscope (SEM) imaging, inhibiting kinetics curves, urea fast test, cell counting kit-8 (CCK-8) assay, western blot analysis, griess reagent system, and molecular docking. Our results showed that chebulinic acid effectively inhibited the growth of the HP strain ATCC 700392, damaged the HP structure, and exhibited selective antimicrobial activity without affecting normal epithelial cells GES-1. Importantly, it suppressed the expression of Cytotoxin-associated gene A (Cag A) protein, a crucial factor in HP infection. Molecular docking analysis predicted a strong affinity (-9.7 kcal/mol) between chebulinic acid and Cag A protein. Overall, our findings suggest that chebulinic acid acts as an anti-adhesive agent, disrupting the adhesion of HP to host cells, which is a critical step in HP infection. It also suppresses the Cag A protein. These results highlight the potential of chebulinic acid against HP infections.

Emergence and Characterization of the High-Level Tigecycline Resistance Gene tet(X4) in Salmonella enterica Serovar Rissen from Food in China.

The plasmid-mediated tet(X4) gene has exhibited a high-level resistance to tigecycline (TGC), which has raised concerns globally regarding antibiotic resistance. Although the widespread tet(X4) has been found widely in Escherichia coli, it is scarcely found in other Enterobacteriaceae. This study aimed to characterize a ST469 Salmonella enterica serovar Rissen (S. Rissen) isolate harboring tet(X4) from pork, which was identified and characterized via antimicrobial susceptibility testing, conjugation assays, plasmid curing testing, whole-genome sequencing, and bioinformatic analysis. Ten ST469 S. Rissen isolates of 223 Salmonella spp. isolates were isolated from food samples in China during 2021-2023. One of 10 S. Rissen isolates, SM2301, carrying tet(X4) conferred high-level resistance to TGC (minimum inhibitory concentration > 8 µg/mL). The tet(X4) could be conjugated into different recipients, including E. coli, S. enteritidis, and K. pneumoniae isolates. Plasmid curing confirmed that tet(X4) was plasmid-mediated. Genetic analysis revealed that the tet(X4) in the SM2301 isolate was located in the IncFIA(HI1)-IncHI1A-IncHI1B(R27) hybrid plasmid, and the structure of tet(X4) was abh-tet(X4)-ISCR2. To the best of our knowledge, this is the first report of a tet(X4)-positive food-derived S. Rissen isolate. The extending bacterial species of tet(X4)-bearing plasmids suggested the increasing transmission risk of the mobile TGC resistance gene tet(X4) beyond E. coli. This study highlights the emerging and evolution risk of novel resistance genes across various bacterial species. Therefore, further surveillance is warranted to monitor the prevalence of tet(X4) in Salmonella spp. and other bacterial species.

Exploring urban and agricultural land use planning

Socio-economic development has accelerated urban expansion, leading to the encroachment of urban space into agricultural space land and seriously undermining the stability of ecosystems. Scientifically optimizing the layout of urban-agricultural space can guarantee China's food security, promote urban development and maintain ecological stability. In this study, we focused on the rapidly growing city of Nankang. We used land, ecological and geographic location data to calculate land suitability, predicted future urban space through the GeoSOS-FLUS model, and finally optimized the agricultural-urban space conflict. The results show that: the areas of farmland with quality evaluation results for grades 1-4 were 7783.13 hm2, 9411.01 hm2, 8738.99 hm2 and 3032.73 hm2, and the areas suitable for construction, more suitable for construction, unsuitable for construction and prohibited from construction accounted for 12.19%, 16.23%, 41.34% and 30.24%, respectively. Combining the FLUS model with the land suitability evaluation results, we found that there is a conflict area of 1841.96 hm2 in the delineation scheme. Adjusting their layout according to the principle of conflict coordination, the optimized area of agricultural-urban space is 28429.34 hm2 and 8968.23 hm2 respectively, accounting for 16.32% and 5.15% of the study area. The result of spatial contradiction optimization satisfies the balance between production and residential space, promotes urban development, protects ecological security, and can provide scientific reference for regional urban ecological environment construction.

Impact of political relationships on virtual land flow from China's food trade

The increasing backlash against globalization has shifted public opinion towards being against trade. This situation puts food trade, which is resources constrained and susceptible to political relations, into a complex dilemma. Due to the concerns on agricultural endowments, food trade needs to be considered with strategic interests because of its sensitivity and fragility. Using virtual land as a measurement to analyse the resource flows behind the food trade can provide an objective perspective on what extent a country depends on the trade. Overlaying worldwide trade disputes and political interventions, establishing solid trade relationships is increasingly becoming urgent. This study uses an extended gravity model, assesses the virtual land traded by China with 10 species over 20 years, and provides insights on establishing politics-oriented trade relationships, further testing how this political mechanism works in the food trade. The results demonstrate that memorandums of understanding are the fundamental institutional layer to guarantee trading activities and promote smooth transactions for both sides; state visits are determinable factors in establishing bilateral relationships and it significantly promote upper-middle income countries, which crucially impacts the trading system. As those endeavors undertaken by executive departments are often ignored, more attention should be paid to them during interactive stages. Political relations show differences in food trade among different countries and varieties. The promoting effect of political relations is stronger in middle and high-income countries, and the impact on Russia and Ukraine is more significant than others. Compared within varieties, foreign state visits and endeavor presents more positive on pork.