Food Cup Research Articles

Negative attributes of mixed-valence memories strengthen over long retention intervals and the degree of enhancement is predicted by individual differences in state anxiety.

Memories are multifaceted and can simultaneously contain positive and negative attributes. Here, we report that negative attributes of a mixed-valence memory dominate long-term recall. To induce a mixed-valence memory, running responses were randomly reinforced with either food (∼83% of trials) or footshock (∼17% of trials), or a noise conditioned stimulus (CS) was followed randomly with either food (∼80% of trials) or footshock (∼20% of trials). Control animals were consistently reinforced with only food. Mixed-valence training promoted unstable behavior (e.g., erratic approach and withdrawal from the food cup) and moderate levels of fear during the training regimens. After a 20-day retention interval, animals that were consistently reinforced with food exhibited intact approach responding, and similar responding was observed if animals were food deprived or satiated (i.e., the response was insensitive to motivation). However, animals that experienced the mixed-valence training expressed significantly enhanced and stable fear (consistent immobility) relative to the end of training, regardless of whether animals were food deprived or not, suggesting that fear transitioned to a state that was insensitive to motivation. The degree of fear expressed during long-term retention was predicted by measures of state anxiety obtained prior to the training, indicating that the enhancement of fear across the retention interval was related to individual differences in basal "anxiety." These results suggest that negative attributes of memories dominate long-term recall, particularly in animals expressing an anxious phenotype, and these observations have direct implications for the chronic nature of anxiety disorders and the exacerbation of fear that accompanies posttraumatic stress disorder. (PsycInfo Database Record (c) 2023 APA, all rights reserved).

Taurine, a Component of the Tear Film, Exacerbates the Pathogenic Mechanisms of Acanthamoeba castellanii in the Ex Vivo Amoebic Keratitis Model.

Acanthamoeba spp. is the etiological agent of amoebic keratitis. In this study, the effect of taurine in physiological concentrations in tears (195 μM) on trophozoites of Acanthamoeba castellanii through the ex vivo amoebic keratitis model was evaluated. Trophozoites were coincubated with the Syrian golden hamster cornea (Mesocricetus auratus) for 3 and 6 h. Group 1: Control (-). Corneas coincubated with amoebic culture medium and taurine. Group 2: Control (+). Corneas coincubated with trophozoites without taurine. Group 3: Corneas coincubated with taurine 15 min before adding trophozoites. Group 4: Trophozoites coincubated 15 min with taurine before placing them on the cornea. Group 5: Corneas coincubated for 15 min with trophozoites; subsequently, taurine was added. Results are similar for both times, as evaluated by scanning electron microscopy. As expected, in the corneas of Group 1, no alterations were observed in the corneal epithelium. In the corneas of Group 2, few adhered trophozoites were observed on the corneal surface initiating migrations through cell junctions as previously described; however, in corneas of Groups 3, 4 and 5, abundant trophozoites were observed, penetrating through different corneal cell areas, emitting food cups and destabilizing corneal surface in areas far from cell junctions. Significant differences were confirmed in trophozoites adherence coincubated with taurine (p < 0.05). Taurine does not prevent the adhesion and invasion of the amoebae, nor does it favor its detachment once these have adhered to the cornea, suggesting that taurine in the physiological concentrations found in tears stimulates pathogenic mechanisms of A. castellanii.

Decreased Ventral Tegmental Area CB1R Signaling Reduces Sign Tracking and Shifts Cue-Outcome Dynamics in Rat Nucleus Accumbens.

Sign-tracking (ST) rats show enhanced cue sensitivity before drug experience that predicts greater discrete cue-induced drug seeking compared with goal-tracking or intermediate rats. Cue-evoked dopamine in the nucleus accumbens (NAc) is a neurobiological signature of sign-tracking behaviors. Here, we examine a critical regulator of the dopamine system, endocannabinoids, which bind the cannabinoid receptor-1 (CB1R) in the ventral tegmental area (VTA) to control cue-evoked striatal dopamine levels. We use cell type-specific optogenetics, intra-VTA pharmacology, and fiber photometry to test the hypothesis that VTA CB1R receptor signaling regulates NAc dopamine levels to control sign tracking. We trained male and female rats in a Pavlovian lever autoshaping (PLA) task to determine their tracking groups before testing the effect of VTA → NAc dopamine inhibition. We found that this circuit is critical for mediating the vigor of the ST response. Upstream of this circuit, intra-VTA infusions of rimonabant, a CB1R inverse agonist, during PLA decrease lever and increase food cup approach in sign-trackers. Using fiber photometry to measure fluorescent signals from a dopamine sensor, GRABDA (AAV9-hSyn-DA2m), we tested the effects of intra-VTA rimonabant on NAc dopamine dynamics during autoshaping in female rats. We found that intra-VTA rimonabant decreased sign-tracking behaviors, which was associated with increases in NAc shell, but not core, dopamine levels during reward delivery [unconditioned stimulus (US)]. Our results suggest that CB1R signaling in the VTA influences the balance between the conditioned stimulus-evoked and US-evoked dopamine responses in the NAc shell and biases behavioral responding to cues in sign-tracking rats.SIGNIFICANCE STATEMENT Substance use disorder (SUD) is a chronically relapsing psychological disorder that affects a subset of individuals who engage in drug use. Recent research suggests that there are individual behavioral and neurobiological differences before drug experience that predict SUD and relapse vulnerabilities. Here, we investigate how midbrain endocannabinoids regulate a brain pathway that is exclusively involved in driving cue-motivated behaviors of sign-tracking rats. This work contributes to our mechanistic understanding of individual vulnerabilities to cue-triggered natural reward seeking that have relevance for drug-motivated behaviors.

Observing a trained demonstrator influences associative appetitive learning in rats.

The ability to acquire information about the environment through social observation or instruction is an essential form of learning in humans and other animals. Here, we assessed the ability of rats to acquire an association between a light stimulus and the presentation of a reward that is either hidden (sucrose solution) or visible (food pellet) via observation of a trained demonstrator. Subsequent training of observers on the light-reward association indicated that while observation alone was not sufficient for observers to acquire the association, contact with the reward location was higher in observers that were paired with a demonstrator. However, this was only true when the light cue predicted a sucrose reward. Additionally, we found that in the visible reward condition, levels of demonstrator orienting and food cup contact during the observation period tended to be positively correlated with the corresponding behaviour of their observer. This relationship was only seen during later sessions of observer training. Together, these results suggest that while our models were not sufficient to induce associative learning through observation alone, demonstrator behaviour during observation did influence how their paired observer's behavioural response to the cue evolved over the course of direct individual training.

Copy rats: Learning by observation during a foraging task by rats.

In two experiments, rats observed expert foragers in a laboratory foraging task. In both experiments, 12 towers with a food cup on top of each tower were placed in a circle. Six towers, marked with black and white stripes, had cups baited with cheese, and were located in randomly selected positions on successive trials. The other six towers were white with food cups that were sham baited with inaccessible food. In both experiments, during Phase 1, demonstrator rats eventually learned to find the baited towers, making approximately 90% correct choices in their first six choices. In Phase 2, observer rats each had an opportunity to observe, from a cage located inside the circle of towers, a now-expert demonstrator forage. Half of the observers were able to observe a cage mate, whereas the other half of the observers were able to observe a non-cage mate. After a delay of about 2 min (Experiment 1) or about 24 h (Experiment 2), the observers were allowed to forage among the rebaited towers. In both experiments, the observers performed better during their 20 Phase 2 trials than the demonstrators had performed during their first 20 Phase 1 trials. But, in both experiments, there was no clearly significant difference between the performance of observers able to watch cage mates as opposed to non-cage mates. Because the observational effect seen in both experiments survived a 24-h delay between observation and performance, it was deemed to have been based on learning.

The role of nutrient‐responsive hippocampal neurons in feeding

Feeding behavior is controlled by hippocampal‐dependent learning and memory that influence decisions about where, when and what to eat. However, the neuronal subtypes and stimuli that recruit these neurons remain poorly understood.Here we leverage the unique strength of the recently‐developed Targeted Recombination in Activated Populations (TRAP2) mouse model, which provides cell type‐unbiased genetic access to neuronal populations based on their activation by defined and time‐constrained stimuli. Surprisingly, after comparing neuronal activity to separate post‐ingestive stimuli within the same animal, we identified distinct hippocampal subpopulations that respond to either fat or sugar. We validated a protocol for selective ablation of the sugar‐activated hippocampal neurons using viral mediated caspase in the TRAP2 mice. Using an automated feeding system, we found that these sugar‐ablated mice significantly reduced their chow and high‐fat diet intake compared to control littermates, and exhibited impaired food location memory in a sugar‐driven food cup task. No group differences were observed in the ability to learn a flavor‐nutrient conditioning paradigm.Overall, these results demonstrate that the hippocampus is attuned to specific macronutrients rather than the feeding state, allowing highly‐refined appetitive learning and memory in response to context‐specific feeding cues.

Tray forming operation of paperboard: A case study using implicit finite element analysis

AbstractThe possibility to perform advanced forming operations of initially plane paperboard paves the way to making products like food trays, plates, cups and other containers as a part of shifting towards a circular bioeconomy. As a part of the ongoing efforts of expanding the product range using paperboard, we performed analyses of the forming operation using simulations. An implicit non‐linear finite element model is built to more accurately than previous studies simulate the tray forming process of paperboard. Two different commercial paperboards are investigated. The use of an implicit solver enabled the inclusion of the creasing pattern into the geometry of the paperboard blank resolving the formation of wrinkles during forming. The material data is extracted from tensile test curves of the investigated paperboards and was fitted accurately using Hill's plasticity with difference in tension and compression accounted for with subsequent failure evaluation. The results showed that the inclusion of the creases in the geometry is vital for getting a correct shape of the formed tray and important for decreasing the risk of failure. The results also showed that friction has a big impact on the formed shape and hence on the stress levels, and therefore supports the means of lowering friction between the blank holder and the blank during the tray forming operation. A stochastic approach is proposed to determine the probability of failure for the boards. The performed failure evaluation is consistent with the field observations. The developed approach enables more precise simulations of paperboard tray forming.

Cigarette butts in beach litter: Snapshot of a summer holiday

Cigarette butts are considered the most common form of personal litter around the world. It is estimated that 5.5 trillion cigarettes are consumed globally each year and that 4.95 trillion are discarded in some natural or anthropic environment around the world. This study evaluated the pollution of urban beaches in the Brazilian Northeast by cigarette butts in relation to other types of litter. Samplings were conducted in a month of high season (January 2016), at eight heavily used beaches. In the place with the highest concentration of users in each beach, a 200 m transect (3 m width) was established parallel to the water on the most recent strandline. All items >1 cm were counted and classified into categories according to composition (plastic, cigarette butts, wood, glass, metal, paper and organic, such as food leftovers and coconuts) using a standard worksheet. Cigarette butts were considered as an isolated category of litter. A total of 10,880 items was registered. The most abundant items were plastics (44.96%) and cigarette butts (38.36%). Among plastics, the most abundant items were straws, metallic food packaging, sticks (lollipop and popsicle) and cups. Plastic fragments were also abundant in all beaches evaluated, corresponding to 14.5% of the total plastics.

Appetitive and aversive sensory preconditioning in rats is impaired by disruption of the postrhinal cortex

Episodic memory involves binding stimuli and/or events together in time and place. Furthermore, memories become more complex when new experiences influence the meaning of stimuli within the original memory. Thus collectively, complex episodic memory formation and maintenance involves processes such as encoding, storage, retrieval, updating and reconsolidation, which can be studied using animal models of higher-order conditioning. In the present study aversive and appetitive sensory preconditioning paradigms were used to test the hypothesis that the postrhinal cortex (POR), which is a component of the hippocampal memory system, is involved in higher-order conditioning. Drawing on the known role of the POR in contextual learning, Experiment 1 employed a four-phase sensory preconditioning task that involved fear learning and context discrimination in rats with or without permanent lesions of the POR. In parallel, to examine POR function during higher-order conditioning in the absence of a particular spatial arrangement, Experiments 2 and 3 used a three-phase sensory preconditioning paradigm involving phasic stimuli. In Experiment 2, bilateral lesions of the POR were made and in Experiment 3, a chemogenetic approach was used to temporarily inactivate POR neurons during each phase of the paradigm. Evidence of successful sensory preconditioning was observed in sham rats which, during the critical context discrimination test, demonstrated higher levels of freezing behavior when re-exposed to the paired versus the unpaired context, whereas POR-lesioned rats did not. Data from the appetitive sensory preconditioning paradigm also confirmed the hypothesis in that during the critical auditory discrimination test, sham rats showed greater food cup responding following presentations of the paired compared to the unpaired auditory stimulus, whereas POR-lesioned rats did not. Lastly, in Experiment 3, when the POR was inactivated only during preconditioning or only during conditioning, discrimination during the critical auditory test was impaired. Thus, regardless of whether stimulus-stimulus associations were formed between static or phasic stimuli or whether revaluation of the paired stimulus occurred through association with an aversive or an appetitive unconditioned stimulus, the effects were the same; POR lesions disrupted the ability to use higher-order conditioned stimuli to guide prospective behavior.

New Jersey bans plastic and paper bags

Shoppers in New Jersey who don’t already bring reusable bags to stores would do well to begin practicing. Starting in May 2022, both plastic and paper single-use bags will be banned, along with polystyrene foam food containers and cups. Gov. Phil Murphy signed legislation for one of the strictest plastics bans in the US, S864 , into law in early November. In the law, the New Jersey Legislature determined that “it is no longer conscionable to permit the unfettered use and disposal of single-use plastics in the State.” It cited the need for the state, with nearly 9 million residents, to minimize plastic pollution entering the ocean. Lawmakers included paper bags in the ban after finding that “single-use paper carryout bags use as much or more energy and resources to manufacture and transport than single-use plastic carryout bags and contribute to harmful air emissions.” The law encourages the distribution of

Reward-predictive cues elicit excessive reward seeking in adolescent rats

Impulsive behavior during adolescence may stem from developmental imbalances between motivational and cognitive-control systems, producing greater urges to pursue reward and weakened capacities to inhibit such actions. Here, we developed a Pavlovian-instrumental transfer (PIT) protocol to assay rats’ ability to suppress cue-motivated reward seeking based on changes in reward expectancy. Traditionally, PIT studies focus on how reward-predictive cues motivate instrumental reward-seeking behavior (lever pressing). However, cues signaling imminent reward delivery also elicit countervailing focal-search responses (food-port entry). We first examined how reward expectancy (cue-reward probability) influences expression of these competing behaviors. Adult male rats increased rates of lever pressing when presented with cues signaling lower probabilities of reward but focused their activity at the food cup on trials with cues that signaled higher probabilities of reward. We then compared adolescent and adult male rats in their responsivity to cues signaling different reward probabilities. In contrast to adults, adolescent rats did not flexibly adjust patterns of responding based on the expected likelihood of reward delivery but increased their rate of lever pressing for both weak and strong cues. These findings indicate that control over cue-motivated behavior is fundamentally dysregulated during adolescence, providing a model for studying neurobiological mechanisms of adolescent impulsivity.

Environmental impact of a new concept of food service: A case study for the re-use of naval shipping containers

This study aims to evaluate the environmental sustainability of a new street food format for food service based on the re-use of naval shipping containers and to compare it with the conventional one (street food truck). The environmental impact analysis was performed using the Life Cycle Assessment methodology. The functional unit (FU) was identified in the food service, including three food preparations: a dish of pasta (100 g), one sandwich (150 g) and one portion of fries (200 g). Following a “from cradle to gate” approach, the factors studied are: (i) the customization of a shipping container in a street food format, (ii) the construction and use of the cooking appliance, (iii) the logistics, (iv) the cooking phase (including final packaging as food cup). The life cycle of ingredients for food preparations has been neglected due to the variability of the products.The results show that the two higher hotspots are electricity consumed by cooking appliance (35%) and oil used to fry (34%), attributable only to the fries preparation. The third hotspot is imputable to the customized structure, with an average percentage value equal to 15%. Considering the global warming impact category, the customization into a street food format release 1280 kg CO2eq, while the production of a new container or a new street food truck format implies the emission of 12,800 kg CO2eq and 20,900 kg CO2eq respectively. The impact of the customized container (re-used container) weight for 0.04 kg CO2eq/FU, this value increases 11.6 times for a new container street food format, and 17 times for a new street food truck format.Overall, quantifying the environmental damage, the results showed how the re-use of a naval shipping container can be a way to reduce the environmental impact of food preparation, avoiding dismissing or building activity of the structure reducing the impact of the structure of about 95% offering a more sustainable street food services.

Perinatal exposure of rats to a maternal diet with varying protein quantity and quality affects the risk of overweight in female adult offspring

The maternal protein diet during the perinatal period can program the health of adult offspring. This study in rats evaluated the effects of protein quantity and quality in the maternal diet during gestation and lactation on weight and adiposity in female offspring. Six groups of dams were fed a high-protein (HP; 47% protein) or normal-protein (NP; 19% protein) isocaloric diet during gestation (G) using either cow's milk (M), pea (P) or turkey (T) proteins. During lactation, all dams received the NP diet (protein source unchanged). From postnatal day (PND) 28 until PND70, female pups (n=8) from the dam milk groups were exposed to either an NP milk diet (NPMW) or to dietary self-selection (DSS). All other pups were only exposed to DSS. The DSS design was a choice between five food cups containing HPM, HPP, HPT, carbohydrates or lipids. The weights and food intakes of the animals were recorded throughout the study, and samples from offspring were collected on PND70. During the lactation and postweaning periods, body weight was lower in the pea and turkey groups (NPG and HPG) versus the milk group (P<.0001). DSS groups increased their total energy and fat intakes compared to the NPMW group (P<.0001). In all HPG groups, total adipose tissue was increased (P=.03) associated with higher fasting plasma leptin (P<.05). These results suggest that the maternal protein source impacted offspring body weight and that protein excess during gestation, irrespective of its source, increased the risk of adiposity development in female adult offspring.

Lesions to the lateral mammillary nuclei disrupt spatial learning in rats.

The head direction (HD) signal is thought to originate in the reciprocal connections between the dorsal tegmental nuclei (DTN) and the lateral mammillary nuclei (LMN) and lesions to these structures disrupt the HD signal in downstream structures. Lesions to the DTN also disrupt performance on spatial tasks where directional heading is thought to be important. In Experiment 1, rats with bilateral electrolytic lesions of the LMN and sham controls were trained on 2 tasks previously shown to be sensitive to DTN damage. Rats were first trained on either a direction or rotation problem in a water T maze. LMN-lesioned rats were impaired relative to sham controls, on both the first block of 8 trials and on the total number of trials taken to reach criterion. In the food-foraging task, rats were trained to leave a home cage at the periphery of a circular table, find food in a food cup at the center of the table, and return to the home cage. Again, LMN-lesioned rats were impaired relative to sham rats, making more errors on the return component of the foraging trip. In Experiment 2, rats with electrolytic LMN lesions were also impaired on a dry land version of the direction and rotation problems and had difficulty discriminating between reinforced and nonreinforced locations on a 12-arm maze. These results build on previous behavioral and cell-recording studies and demonstrate the importance of the direction system to spatial learning. (PsycINFO Database Record (c) 2019 APA, all rights reserved).

Naegleria fowleri: Sources of infection, pathophysiology, diagnosis, and management; a review.

Naegleria fowleri, a thermophilic flagellate amoeba known as a "brain-eating" amoeba, is the aetiological agent of a perilous and devastating waterborne disease known as primary amoebic meningoencephalitis (PAM), both in humans as well as in animals. PAM is a rare but fatal disease affecting young adults all around the world, particularly in the developed world but recently reported from developing countries, with 95%-99% mortality rate. Swimmers and divers are at high risk of PAM as the warm water is the most propitious environment adapted by N.fowleri to cause this infection. Infective amoeba in the trophozoite phase enter the victim's body through the nose, crossing the cribriform plate to reach the human brain and cause severe destruction of the central nervous system (CNS). The brain damage leads to brain haemorrhage and death occurs within 3-7days in undiagnosed cases and maltreated cases. Though the exact pathogenesis of N.fowleri is still not known, it has exhibited two primary mechanisms, contact-independent (brain damage through different proteins) and contact-dependent (brain damage through surface structures food cups), that predominantly contribute to the pathogen invading the host CNS. For the management of this life-threatening infection different treatment regimens have been applied but still the survival rate is only 5% which is ascribed to its misdiagnosis, as the PAM symptoms closely resembled bacterial meningitis. The main objectives of this review article are to compile data to explore the sources and routes of N.fowleri infection, its association in causing PAM along with its pathophysiology; latest techniques used for accurate diagnosis, management options along with challenges for Pakistan to control this drastic disorder.

Dog owner’s accuracy measuring different volumes of dry dog food using three different measuring devices

Prior research demonstrates significant inaccuracy when repeatedly measuring the same amount of dry dog food using a dry-food measuring cup, bringing into question the accuracy of measuring devices. This study...

Impacts of inter-trial interval duration on a computational model of sign-tracking vs. goal-tracking behaviour

In the context of Pavlovian conditioning, two types of behaviour may emerge within the population (Flagel et al. Nature, 469(7328): 53–57, 2011). Animals may choose to engage either with the conditioned stimulus (CS), a behaviour known as sign-tracking (ST) which is sensitive to dopamine inhibition for its acquisition, or with the food cup in which the reward or unconditioned stimulus (US) will eventually be delivered, a behaviour known as goal-tracking (GT) which is dependent on dopamine for its expression only. Previous work by Lesaint et al. (PLoS Comput Biol, 10(2), 2014) offered a computational explanation for these phenomena and led to the prediction that varying the duration of the inter-trial interval (ITI) would change the relative ST-GT proportion in the population as well as phasic dopamine responses. A recent study verified this prediction, but also found a rich variance of ST and GT behaviours within the trial which goes beyond the original computational model. In this paper, we provide a computational perspective on these novel results.

Effect of Live Lactobacillus Paracasei NFRI 7415 on the Preference for a Lard Diet or Fish Oil Diet in Rats

&lt;p&gt;&lt;em&gt;This study examined the effect of live &lt;em&gt;&lt;em&gt;Lactobacillus paracasei&lt;/em&gt;&lt;/em&gt; NFRI 7415 on the preference of a lard diet (LD) and a fish-oil diet (FD) in rats. 4-week-old male Fischer 344 rats were fed one of four diets; LD, LD + lactic acid bacteria (LLD), FD and FD + lactic acid bacteria for 4 weeks (dietary experimental period). The LLD and FLD groups freely ingested water containing &lt;em&gt;&lt;em&gt;Lb. paracasei&lt;/em&gt;&lt;/em&gt; NFRI 7415 (10&lt;sup&gt;7&lt;/sup&gt; cfu/ml). After 4 weeks, all rats were placed on a two-choice diet program in which they self-selected from two food cups, each containing either the LD or the FD for 5 weeks (self-selection period). After the dietary experimental period, there was no significant difference in the final body weight and total food intake among the four groups. The intake of fish-oil and live &lt;em&gt;&lt;em&gt;Lb. paracasei&lt;/em&gt;&lt;/em&gt; NFRI 7415 was increasing the fecal lipids excretion, and it effectively reduced plasma total cholesterol concentration (p&amp;lt;0.05). It was indicated that the intake of live &lt;em&gt;&lt;em&gt;Lb. paracasei&lt;/em&gt;&lt;/em&gt; NFRI 7415 was no influence on the preference for fat in the dietary experimental period and the self-selection period.&lt;/em&gt;&lt;/p&gt;

Manipulating the revision of reward value during the intertrial interval increases sign tracking and dopamine release.

Recent computational models of sign tracking (ST) and goal tracking (GT) have accounted for observations that dopamine (DA) is not necessary for all forms of learning and have provided a set of predictions to further their validity. Among these, a central prediction is that manipulating the intertrial interval (ITI) during autoshaping should change the relative ST-GT proportion as well as DA phasic responses. Here, we tested these predictions and found that lengthening the ITI increased ST, i.e., behavioral engagement with conditioned stimuli (CS) and cue-induced phasic DA release. Importantly, DA release was also present at the time of reward delivery, even after learning, and DA release was correlated with time spent in the food cup during the ITI. During conditioning with shorter ITIs, GT was prominent (i.e., engagement with food cup), and DA release responded to the CS while being absent at the time of reward delivery after learning. Hence, shorter ITIs restored the classical DA reward prediction error (RPE) pattern. These results validate the computational hypotheses, opening new perspectives on the understanding of individual differences in Pavlovian conditioning and DA signaling.

Cannabinoid receptor-1 signaling contributions to sign-tracking and conditioned reinforcement in rats.

Endocannabinoids (eCBs) are critical gatekeepers of dopaminergic signaling, and disrupting cannabinoid receptor-1 (CB1) signaling alters DA dynamics to attenuate cue-motivated behaviors. Prior studies suggest that dopamine (DA) release plays a critical role in driving sign-tracking. Here, we determine whether systemic injections of rimonabant, a CB1 receptor inverse agonist, during Pavlovian lever autoshaping impair the expression of sign-tracking. We next examine whether rimonabant blocks the reinforcing properties of the Pavlovian lever cue in a conditioned reinforcement test. In Exp. 1, we trained rats in Pavlovian lever autoshaping prior to systemic rimonabant injections (0, 1, 3mg/kg) during early and late Pavlovian lever autoshaping sessions. In Exp. 2, we trained rats in Pavlovian lever autoshaping prior to systemic rimonabant injections (0, 1mg/kg) during a conditioned reinforcement test. Rimonabant dose-dependently decreased lever contact and probability, and increased sign-tracker's latency to approach the lever cue early in Pavlovian training. With extended training, many previously goal-tracking and intermediate rats shifted to lever approach, which remained dose-dependently sensitive to rimonabant. Rimonabant attenuated cue-evoked food cup approach early, but not late, in conditioning, and did not affect pellet retrieval or consumption. The inserted lever cue served as a robust conditioned reinforcer after Pavlovian lever autoshaping, and 1mg/kg rimonabant blocked conditioned reinforcement. Together, our results suggest that CB1 signaling mediates two critical properties of incentive stimuli; their ability to attract (Exp. 1) and their ability to reinforce (Exp. 2) behavior.