This study discusses evaluating employee performance in microbiology laboratories using an association matrix implemented in web-based software with the Streamlit framework. The purpose of the research is to improve the employee performance evaluation process, which previously used conventional methods. This software is built from a sample receipt recording history data stored in a MySQL database. The initially unstructured data was processed using Python libraries such as NumPy, Matplotlib, Pandas, and Difflib to generate personnel evaluation information such as specialization, task duration, workload, and individual competencies. This software can provide a fast and accurate performance assessment according to the evaluation period. In a test with the System Usability Scale (SUS), the software scored 75.83, which was rated "good.". These results show that the software is easy to use and can improve the efficiency of employee performance evaluation. Follow-up tests with questionnaires given to 18 users showed that this system was preferable to previous conventional methods. This software helps laboratory managers evaluate employee performance effectively and efficiently.