Follicular Tissue Research Articles

Short Communication Molecular cloning and expression pattern of the porcine 5-aminolevulinate synthase 1 (ALAS1) gene and its association with reproductive traits

5-Aminolevulinate synthase 1 (ALAS1) is the first enzyme in the heme biosynthetic pathway and is upregulated in follicular tissue during the early stages of ovulation. In this study, we isolated the complete coding sequence of the porcine ALAS1 gene and its 2-9 intron sequence, identified a single nucleotide polymorphism (SNP; T/C) in intron 9, and developed a PCR-MspI-restriction fragment length polymorphism genotyping assay. Association of the SNP with litter size was assessed in two populations [purebred Large White and the experimental synthetic (DIV) line]. Statistical analysis demonstrated that for total number of piglets born (TNB) in all parities, pigs with the CC genotype had an additional 0.68 and 0.74 piglets compared to the TC and TT animals (P < 0.05) in the DIV line, respectively. Purebred Large White sows inheriting the CC and TC genotypes gave birth to an additional 0.96 and 0.70 piglets compared to the TT animals (P < 0.05) in all parities, respectively. In addition, for TNB in all parities, a significant additive effect of 0.48 ± 0.23 and 0.37 ± 0.17 piglets/ litter was detected in sows of both populations (P < 0.05), respectively. The highest levels of ALAS1 gene expression were observed in isolated ovarian granulosa cells 2 and 12 h after stimulation with pregnant mare serum gonadotropin human chorionic gonadotropin, which represents the time of follicular development and ovulation, respectively. Therefore, the ALAS1 gene was significantly associated with litter size in two populations and could be a useful molecular marker for the selection of increasing litter size in pigs.

Autotransplantation donor tooth site harvesting using piezosurgery.

Background:The harvesting of a tooth as a candidate for tooth autotransplantation requires that the delicate dental tissues around the tooth be minimally traumatized. This is especially so for the periradicular tissues of the tooth root and the follicular tissues surrounding the crown. The aim of this report is to describe the use of piezosurgery as an attempt at morbidity reduction in the harvesting of teeth for autotransplantation.Methods:A piezosurgical handpiece and its selection of tips were easily adapted to allow the harvesting and delivery of teeth for autotransplantation purposes.Results:Twenty premolar teeth were harvested using a piezosurgical device. The harvested teeth were subsequently successfully autotransplanted. All twenty teeth healed in a satisfactory manner without excessive mobility or ankyloses.Conclusions:Piezosurgery avoids some of the traumatic aspects of harvesting teeth and removing bone which are associated with thermal damage from the use of conventional rotary instruments or saws. Piezosurgery can be adapted to facilitate the predictable harvesting of teeth for autotransplantation purposes.

OCT4 and SOX2 are reliable markers in detecting stem cells in odontogenic lesions

Context (Background): Stem cells are a unique subpopulation of cells in the human body with a capacity to initiate differentiation into various cell lines. Tumor stem cells (TSCs) are a unique subpopulation of cells that possess the ability to initiate a neoplasm and sustain self-renewal. Epithelial stem cell (ESC) markers such as octamer-binding transcription factor 4 (OCT4) and sex-determining region Y (SRY)-box 2 (SOX2) are capable of identifying these stem cells expressed during the early stages of tooth development. Aims: To detect the expression of the stem cell markers OCT4 and SOX2 in the normal odontogenic tissues and the odontogenic cysts and tumors. Materials and Methods: Paraffin sections of follicular tissue, radicular cyst, dentigerous cyst, odontogenic keratocyst, ameloblastoma, adenomatoid odontogenic tumor, and ameloblastic carcinoma were obtained from the archives. The sections were subjected to immunohistochemical assay by the use of mouse monoclonal antibodies to OCT4 and SOX2. Statistical Analysis: The results were evaluated by descriptive analysis. Results: The results show the presence of stem cells in the normal and lesional tissues with these stem cell identifying markers. SOX2 was found to be more consistent and reliable in the detection of stem cells. Conclusion: The stem cell expressions are maintained in the tumor transformation of tissue and probably suggest that there is no phenotypic change of stem cells in progression from normal embryonic state to its tumor component. The quantification and localization reveals interesting trends that indicate the probable role of the cells in the pathogenesis of the lesions.

A Rapid Detection of Human Follicular Tissues at the Crime Scene: A Portable and Digital Solution to Current Backlog of Rape Kits

A Rapid Detection of Human Follicular Tissues at the Crime Scene: A Portable and Digital Solution to Current Backlog of Rape Kits

Histopathological and Radiographic Analysis of Dental Follicle of Impacted Teeth Using Modified Gallego's Stain.

In the WHO classification of odontogenic tumours, hard tissue formation has been considered as a sub-classification however, this parameter has not been much explored in dental follicle in literature. Epithelial-mesenchymal interactions play an important role in odontogenesis and its associated pathologies; therefore research on dental follicle should also include mesenchymal components along with epithelial components. Additionally, special stains to identify the nature of such depositions in dental follicle have been less explored. Modified Gallego's stain is such an example which has not been tried in odontogenic lesions which makes this study unique. Aim of this study was to study histopathological variations in dental follicle, the nature of calcification and depositions using Modified Gallego's stain and to correlate histological features of dental follicle with pericoronal width radiographically. A prospective histological study of the dental follicles of 50 impacted teeth was carried out to microscopically evaluate the dental follicular tissues for pathological changes, and to correlate it with pericoronal radiolucency. Impacted teeth with pericoronal radiographic width less than 3mm were included in the study and symptomatic teeth were excluded. Further Modified Gallego stain was used to differentiate the nature of hard tissue formation in dental follicle tissues. Dental follicle histologically showed pathological changes resembling dentigerous cyst, ameloblastoma, odontogenic fibroma (Simple and WHO Type), clear cell odontogenic tumour, neurofibroma, neurilemmoma and mucoepidermoid carcinoma. The dental follicle surrounding an impacted tooth has the potential to differentiate into a wide variety of tissue types, and thus shows the potential for cyst and tumour development which was observed in this study in most of the specimens with normal follicular width radiographically.

Incidental findings of thyroid tissue in cervical lymph nodes: old controversy not yet resolved?

The clinical significance of papillary or follicular thyroid tissue incidentally discovered in cervical lymph nodes during pathological assessment of neck dissections for non-thyroid cancers of the upper aero-digestive tract is critically reviewed. Special emphasis is given to controversies over normal-looking, nodal, thyroid follicles. Arguments for and against the benign nature of these follicles are considered together with processes that could be involved in their formation. The admittedly limited evidence suggests that benign, thyroid follicular inclusions rarely occur in cervical lymph nodes. Histological criteria that could be helpful in recognizing the inclusions, which include assessing their extent in conjunction with the size of the node, are discussed. Finally, an algorithm based on collaboration between specialists, correlating histological findings with imaging and loco-regional control of the upper aero-digestive tract cancer, is suggested for the management of patients with incidentally discovered, nodal thyroid tissue.

Pathological features of the structure of the female genital organs from fetuses of mothers with complicated pregnancies

Aim. In the course of the study the structural features of fetal ovarian of mothers with severe preeclampsia in comparison with fetuses from healthy mothers were revealed. Methods and results. Proliferation of connective tissue; mass mortality of the ovocytes; the prevalence of perishing primordial follicles in the follicular unit were demonstrated histologically. Histochemical methods established reduction in the intensity of the reaction to DNP in the nucleus and in the RNP in the cells cytoplasm. Morphometric method showed significant reduction in the relative volume of the follicular tissue, as well as a significant increase in the relative volume of the interstitial tissue. The described type of fetal ovarian structure of mothers with severe PE corresponds to hypoplastic type and constitutes a violation of the gonads formation in utero. Conclusion. The revealed features of the fetal ovaries structure can contribute to a violation of the germinal function in the further ontogenesis

Intrafollicular expression and potential regulatory role of cocaine- and amphetamine-regulated transcript in the ovine ovary

Follicular growth is regulated by a complex interaction of pituitary gonadotropins with local regulatory molecules. Previous studies demonstrated an important role for cocaine- and amphetamine-regulated transcript (CART) in regulation of granulosa cell estradiol production associated with dominant follicle selection in cattle. However, intraovarian expression and actions of CART in other species, including sheep, are not known. The objective of this study was to investigate the expression of CART in sheep follicles and determine the effects of CART on indices of ovine granulosa cell function linked to follicular development. Results demonstrated the expression of CART messenger RNA and prominent intraovarian localization of CART peptide in granulosa cells of sheep follicles. Granulosa cell CART messenger RNA was lower, but follicular fluid estradiol concentrations were higher in large (>5 mm) follicles vs smaller 3- to 5-mm follicles harvested from sheep ovaries of abattoir origin. CART treatment inhibited follicle stimulating hormone-induced estradiol production by cultured ovine granulosal cells and also blocked the follicle stimulating hormone-induced increase in granulosa cell numbers. Results demonstrate expression of CART in sheep follicular tissues and suggest potential biological actions of CART, which are inhibitory to ovine follicular growth and development.

Determination of plasma kisspeptin concentrations during reproductive cycle and different phases of pregnancy in crossbred cows using bovine specific enzyme immunoassay

Kisspeptin, a decapeptide and potent secretagogue of GnRH has been emerged recently as a master player in the regulation of reproduction in animals. Determination of kisspeptin in peripheral circulation is, therefore, very important for studying the control of its secretion and its role on reproduction in bovine species, the information on which is not available during any physiological state in this species, may probably be due to non-availability of simple assay procedure to measure the hormone. Therefore, the objective of this study was to develop and validate a simple and sufficiently sensitive enzyme immunoassay (EIA) for kisspeptin determination in bovine plasma using the biotin–streptavidin amplification system and second antibody coating technique. Biotin was coupled to kisspeptin and used to bridge between streptavidin-peroxidase and the immobilized kisspeptin antiserum in the competitive assay. The EIA was conducted directly in 100μl of unknown bovine plasma. Kisspeptin standards ranging from 0.01 to 25.6ng/100μl/well were prepared in hormone-free plasma. The lowest detection limit was 0.1ng/ml plasma. Plasma volumes for the EIA, viz., 50, 100 and 200μl did not influence the shape of standard curve even though a drop in OD450 was seen with higher plasma volumes. A parallelism test was carried out to compare the endogenous bovine kisspeptin with kisspeptin standard used. It showed good parallelism with the kisspeptin standard curve. For the biological validation of the assay, plasma kisspeptin was measured in blood samples collected from six non-lactating cyclic cows during entire estrous cycle and from 18 pregnant cows during different stages of pregnancy. The mean plasma kisspeptin concentration during different days of the estrous cycle was different (P<0.001). Three peaks of kisspeptin were recorded, one on a day before appearance of preovulatory LH surge, second at day 6 and third one at day 18 of the estrous cycle. Plasma kisspeptin concentrations increased (P<0.001) from first through last trimester of pregnancy. Kisspeptin concentrations were also measured in different follicular, luteal and placental tissues. Follicular and placental kisspeptin levels increased (P<0.01) during follicular development and with the advancement of pregnancy, respectively. On the other hand, luteal concentrations of kisspeptin decreased (P<0.01) with its developmental process. In conclusion, a simple, sufficiently sensitive and direct EIA procedure has been developed for the first time to determine plasma kisspeptin levels in bovine. A wide range of kisspeptin concentrations can be detected during different physiological stages in bovine using this kisspeptin–EIA procedure.

Histopathological Effect of heavy metal on different organs of fresh water fish tissues from Garmat Ali River adjacent to Al- Najebyia Power Station

Common carp fish was caged from Shatt Al-Arab near Al- Najebyia power station to study the effect of pollution with heavy metals on different fish tissue (gills,kidney, liver, muscle, skin, intestine, spleen ,gonads) sample of the water was analyzed by atomic absorption to find the concentration of heavy metal(ppm=mg/litter) which appear highest than permissible levels. The histopathological results of study were inflammatory cells in skin connective tissue , sub-cutaneous adipose tissue partly with fibrosis,muscle with vacuolation.In gills : Adhesion of some secondary lamella associated with inflammatory cells ,congestion, with loss of epithelial lining only capillaries left, sever atrophy only the remenant of secondary lamella left. Small intestine showed proliferation of mucous screatingcells.Liver had small bile duct enclosed by minimal fibrosis ,portal vein with some inflammatory cells. Kidney revealed glomerulus with high cellularity &amp; tubular vacuolation, reduce hemopoietictissue,peritubularodema, tubular degeneration with congested blood vessel.Spleen revealed central arterioldiletation ,haemorrhage in the tissue,deplitation of follicular aggregation and lymphoid tissue,hemorrhagic red pulp,atrophy of follicular white pulp.gonads had different stages of spermatogenesis in testis and different stages of developing ova , ova with vacuoles of secondary germinal layer.

Smad1 expression in follicular lymphoma.

Background:Follicular lymphomas present with various immunohistologic patterns. The immunohistochemical markers used in the diagnosis of follicular lymphoma show variable degrees of sensitivity and specificity, and thus, additional germinal center markers are required. Smad1 has been reported to be overexpressed in follicular lymphoma, but little is known regarding the expression patterns of Smad proteins in human lymphoid tissue.Methods:In the present study, we performed immunohistochemistry for traditional germinal center markers and for Smad1 in human reactive lymphoid and follicular lymphoma tissues to investigate Smad1’s usefulness in the diagnosis of follicular lymphoma.Results:In the reactive germinal centers, most cells were positive for Smad1. Among the 27 follicular lymphoma cases, 17 of 21 (80%) were Smad1 positive, 17 of 27 (63%) were positive for CD10, and 23 of 27 (85%) were positive for Bcl6. Notably, three cases expressed CD10 only, and one only expressed Bcl6. All these cases were grade 3 tumors and showed follicular and diffuse growth patterns.Conclusions:These results indicate that Smad1 is a candidate as a germinal center marker. Furthermore, they suggest that the Smad signaling pathway might be involved in follicular lymphoma.

Serum antibody immunoreactivity to equine zona protein after SpayVac vaccination

Immunocontraception with porcine ZP (pZP) can be an effective means of fertility control in feral horses. Previous studies suggest that antibodies produced after pZP vaccination may both inhibit fertilization and cause follicular dysgenesis. Zonastat-H, PZP-22, and SpayVac are three pZP vaccines proposed for use in horses. Although all these vaccines contain the pZP antigen, variations in antigen preparation and vaccine formulation lead to differences in antigenic properties among them. Likewise, despite numerous efficacy and safety studies of Zonastat-H and PZP-22, the contraceptive mechanisms of SpayVac remain unclear. The preparation of pZP for SpayVac is thought to include more nonzona proteins, making it less pure than the other two vaccines. This may result in increased antigenicity of the vaccine. We therefore investigated the immunoreactivity of serum antibodies from SpayVac-vaccinated mares to equine zona protein. Western blot analyses revealed an immunoreactivity of these antibodies to protein isolated from mature equine oocytes, ZP, follicular tissues, and ovarian tissues. Immunohistochemical analyses were used to locate the binding of serum antibodies to the ZP of immature oocytes in ovarian stromal tissue. We also found serum antibodies from SpayVac-treated mares to be predominantly specific for zona protein 3. Collectively, our results suggest a model where serum antibodies produced in response to SpayVac vaccination are immunoreactive to equine zona protein in vitro. Our study lends insight into the contraceptive mechanisms underlying the infertility observed after SpayVac vaccination.

Incidência de anormalidades histológicas em tecido correspondente ao espaço pericoronário de terceiros molares inclusos e semi-inclusos

INTRODUÇÃO: O Folículo Pericoronário envolve a coroa do germe dental durante seu desenvolvimento. Quando o dente permanece incluso, alterações do folículo podem originar doenças, como cistos e tumores odontogênicos. OBJETIVO: Analisar as alterações histológicas no tecido mole circundante a terceiros molares inclusos e semi-inclusos, independentemente de alterações patológicas aparentes em suas radiografias correspondentes, além de relacionar o diagnóstico histológico com o diagnóstico radiográfico dos casos. MATERIAL E MÉTODO: A partir de terceiros molares extraídos de 26 pacientes, foram analisados espécimes histológicos de folículos pericoronários por dois examinadores calibrados. O diagnóstico histopatológico obtido foi relacionado ao radiográfico, sendo este realizado por meio de radiografias periapicais e, quando necessário, complementado por radiografias panorâmicas. RESULTADO: Dos 37 folículos pericoronários avaliados, 30% mostraram alterações histológicas compatíveis com cistos dentígeros; 51% eram folículos normais, e 19% continham apenas fragmentos de mucosa. Radiograficamente, 100% dos casos demonstraram características de folículos sem alterações. CONCLUSÃO: Esses dados exemplificam que anormalidades podem estar presentes nos tecidos pericoronários sem que haja evidências clínicas e radiográficas. O exame histopatológico provê um diagnóstico mais preciso e deve ser considerado para a construção do diagnóstico definitivo.

Immunohistochemical expression of keratins 8 and 19 in odontogenic cysts and tumors

Objective: Cytokeratins (CKs) are integral components in the formation of enamel, dentin and/or cemental matrix and their subsequent mineralization. K8 and K19 are known to be chemically homologous during the formation of tooth structures. This feature is rather unique and is expressed across the milieu of odontogenic tissues in the physiological and pathological states. The present study was designed to evaluate the expressions of K8 and K19 in odontogenic lesions immunohistochemically and to test the hypothesis that they can be used to identify the odontogenic epithelium. Study Design: K8 and K19 were assessed in 60 cases of odontogenic lesions retrieved from the archival files, which included ameloblastoma (18), ameloblastic carcinoma (2), adenomatoid odontogenic tumor (AOT) (9), squamous odontogenic tumor (SOT) (1), odontogenic keratocyst (OKC) (10), dentigerous cyst (10), and radicular cyst (10), including two cases of normal gingiva and follicular tissue. Results: K8 was expressed in 53/60 (88.3%) and K19 in 56/60 (93.3%) odontogenic lesions. The labeling for K19 was found to be more intense compared to that for K8. Further, K8 and K19 were found to be positive in all the gingival and follicular tissues. Conclusions: Positive expressions of K8 and K19 in all the odontogenic lesions studied suggest that these can be used as definitive markers for identifying the odontogenic epithelium. However, the intense and consistent expression of K19 suggests that this CK is extremely sensitive in identifying the odontogenic epithelium.

Stem Cells of the Hair Follicular Tissue: Application in Cell Based Therapy for Vitiligo

Stem cells have been found to reside in the different organs of the human body localized in specific niches. One such mini-organ is hair follicle wherein stem cells of epidermal origin have been reported. In the present review article we have reviewed in brief about the melanocyte stem cells of the hair follicle tissue, their identification, surface marker characteristics and role in the cell based therapy for vitiligo.

DENTAL FOLLICLE: ROLE IN DEVELOPMENT OF ODONTOGENIC CYSTS AND TUMOURS Dental Folikül: Odontojenik Kist ve Tümörlerin Oluşumundaki Rolü

ABSTRACT Dental follicle is ecto-mesenchymal derived component of the tooth germ, adjacent to the crown of unerupted tooth. It has many roles during tooth development and eruption. During the impacted tooth surgery, pericoronal and dental follicular tissues are enucleating. However, in rare cases they are submitting for histopathologic evaluation, although it is known, that these tissues are responsible for the occurrence and development of pathologic conditions, such as infections, odontogenic cysts, and tumors.Dental follicle was the subject of many immunohistochemical studies, which have shown a proliferative potential of the dental follicle cells justifying a profilactic removal of the impacted teeth. According to review of relevant literature, the aim of this article is to describe and discuss importance of dental follicle in oral and maxillofacial surgery. Kerwords: Dental follicle, dentigerous cyst, impacted tooth, stem cells OZ Dental folikul dis germini ektomezenkimal bag dokusundan olusturan, disin kronun etrafinda olan ve dis gelisiminde ve surmesinde onemli rol oynayan yapidir. Gomuk7 dis operasyonu sirasinda, pe-rikoronal ve dental folikul dokulari enukle edilmektedir. Ancak bu dokulari, enfeksiyon, odontojenik kist ve tumor gibi patolojik durumlarin gelisiminden sorumlu oldugu halde, nadiren histopatolojik incelemeleri yapilmaktadir. Pek cok immunohistokimyasal calismalar dental folikulu incelerken, onun proliferatif potensiyelini gostererek, gomuk dislerin profilaktik cekimlerini onermektedir. Konu ile ilgili literature gore, bu derlemenin amaci dental folikulun oral ve maksillofasyal cerrahisinde onemli rol oldugunu gostermektir. Anahtar kelimeler: Dental folikul, dentigeroz kist, gomuk dis, kok hucreler

Matrix metalloproteinases are important for follicular development in normal and miniature pigs

To determine the differences in the follicular development process of normal and miniature pigs, we compared the expression of matrix metalloproteinases (MMP) in these two breeds throughout folliculogenesis. In normal pigs, MMP-9 was highly expressed in all stages of the follicular development as well as during ovulation. The follicular development exhibited strong gelatinase activity. The expression of MMP-2 remained insignificant throughout folliculogenesis in both breeds. However, for the follicles of miniature pigs, MMP-2 level was higher than that in normal pigs. Thus MMP types may regulate the remodelling of follicular tissue in the ovaries of normal and miniature pigs. The differential expression of MMPs observed in this study reflected the mechanisms underlying ovarian follicular development in these two breeds.Electronic supplementary materialThe online version of this article (doi:10.1007/s10529-014-1474-9) contains supplementary material, which is available to authorized users.

A New Species ofCyamiocardiumSoot-Ryen, 1951 from Shallow Waters off Brazil, with a Discussion on the Anatomical Characters of the Cyamiidae (Bivalvia: Cyamioidea)

Cyamiocardium domaneschii n. sp. is described from shallow waters off the Southeastern coast of Brazil. An anatomical study provides comparisons with other Cyamioidea. It has a small shell (up to 3.1 mm in length), valves with a rounded contour, external radial sculpture, a distinct large prodissoconch, and hinge structure characteristics of the genus Cyamiocardium Soot-Ryen, 1951. Three well-defined pallial openings are present; tentacles border the anteriormost part of the pedal opening and the inhalant and exhalant apertures. The inner demibranchs are larger than the outers, do not have interlamellar junctions, and form a spacious brooding chamber. It is dioecious and developing oocytes are covered by a thin follicular tissue, which persists as a covering of each brooded young. Offspring are retained up to the juvenile stage, and are attached to the gill filaments by a long stalk. The foot has a narrow, grooved ventral sole; no byssus threads were observed. An active burrowing or crawling behavior is suggested for C. domaneschii n. sp., as there are well developed statocysts. This finding of a cyamiid from Brazil represents the extension of the geographical distribution of this family further north in the southwestern Atlantic Ocean. This study also reveals that some anatomical characters are variable in the genus Cyamiocardium: in C. denticulatum (Smith, 1907), there are only two pallial openings and its foot does not have a ventral sole. The attachment of young to the gills are not related to nourishment, and here for C. domaneschii n. sp. it is suggested that the stalk is only used as a “safety belt”, preventing embryos and juveniles to be released before the end of their development. More studies remain to be done on the anatomy of cyamioideans, particularly on living specimens; we paid attention to common characters used for discussions on the taxonomy and biology of these bivalves.

Evaluation and comparison of expression of p63 in odontogenic keratocyst, solid ameloblastoma and unicystic ameloblastoma.

Background and Objectives:The behavior of odontogenic lesions varies with some tumors behaving like a cyst and some cysts behaving like tumors. p63, a member of the p53 family of tumor suppressor genes has recently come into light in view of its role as an oncogene. The aim of the present study was to investigate the expression of p63 protein in OKC, Solid ameloblastoma, Unicystic Ameloblastoma and Follicular tissue.Materials and Methods:p63 expression was compared in 12 cases of OKC, 12 Solid Ameloblastoma, 14 cases of Unicystic ameloblastoma and 10 cases of Follicular tissue using immunohistochemical technique. All 48 cases were subjected to heat-induced antigen retrieval method using citrate buffer in a pressure cooker. Then the sections were stained with anti-p63 polyclonal antibody and visualized using super sensitive polymer HRP detection system. In each case, number of cells showing p63 positivity were assessed in two compartments - basal and suprabasal and compared.Results:Statistical analysis showed that p63 expression in the suprabasal compartment in Odontogenic keratocysts was equivalent to that of central neoplastic cells of Solid Ameloblastoma and Unicystic Ameloblastoma type 3. Statistically significant difference in the expression of p63 was observed between OKC and Unicystic Ameloblastoma Type 1 and Solid Ameloblastoma and Unicystic Ameloblastoma Type 1.Conclusion:We conclude that the higher expression of p63 in these odontogenic lesions correlates well with their aggressive behavior and thereby suggesting alterations in treatment modalities.

Immunohistochemical expression of Type IV Collagen and Autocrine Motility Factor Receptor in Odontogenic Tumours.

Autocrine motility factor receptor (AMFR) is a tumour motility stimulating protein secreted by tumour cells. The protein encoded by this gene is a glycosylated transmembrane protein and a receptor for autocrine motility factor. It has been known to play a role in progression of neoplastic lesions. Basement membranes are specialized extracellular matrices that serve as structural barriers as well as substrates for cellular interactions. The network of type IV collagen is thought to define the scaffold integrating other components such as laminins and perlecan into highly organized supramolecular architecture. The aim of this study was to determine and evaluate the immunohistochemical expression of Type IV Collagen and Autocrine motility factor receptor in odontogenic lesions. Immunohistochemical expression of Type IV Collagen and Autocrine motility factor receptor was evaluated in 31 odontogenic lesions, including unicystic ameloblastoma, multicystic ameloblastoma, keratocystic odontogenic tumour and ameloblastic carcinoma. Normal follicular tissue formed the control. Maximum expression for Type IV Collagen was seen in multicystic ameloblastoma and minimum expression in keratocystic odontogenic tumour. The maximum expression of AMFR was seen in ameloblastic carcinoma and minimum expression in multicystic ameloblastoma. The results of this study suggested an association of loss of expression of type IV Collagen with progression of lesion. AMFR expression was found to be associated with the aggressive potential of tumours.