Foliar Water Uptake Research Articles

Materials on Plant Leaf Surfaces Are Deliquescent in a Variety of Environments.

Materials on plant leaf surfaces that attract water impact penetration of foliar-applied agrochemicals, foliar water uptake, gas exchange, and stomatal density. Few studies are available on the nature of these substances, and we quantify the hygroscopicity of these materials. Water vapor sorption experiments on twelve leaf washes of sample leaves were conducted and analyzed with inductively coupled plasma-optical emission spectroscopy (ICP-OES) and X-ray diffraction. All leaf surface materials studied were hygroscopic. Oils were found on the surface of the Eucalyptus studied. For mangroves that excrete salt to the leaf surfaces, significant sorption occurred at high humidity of a total of 316 mg (~0.3 ml) over 6–10 leaves and fitted a Guggenheim, Anderson, and de Böer sorption isotherm. Materials on the plant leaf surface can deliquesce and form an aqueous solution in a variety of environments where plants grow, including glasshouses and by the ocean, which is an important factor when considering plant-atmosphere relations.

Tracheid buckling buys time, foliar water uptake pays it back: Coordination of leaf structure and function in tall redwood trees.

Tracheid buckling may protect leaves in the dynamic environments of forest canopies, where rapid intensifications of evaporative demand, such as those brought on by changes in light availability, can result in sudden increases in transpiration rate. While treetop leaves function in reliably direct light, leaves below the upper crown must tolerate rapid, thermally driven increases in evaporative demand. Using synchrotron-based X-ray microtomography, we visualized impacts of experimentally induced water stress and subsequent fogging on living cells in redwood leaves, adding ecological and functional context through crown-wide explorations of variation in leaf physiology and microclimate. Under drought, leaf transfusion tracheids buckle, releasing water that supplies sufficient temporal reserves for leaves to reduce stomatal conductance safely while stopping the further rise of tension. Tracheid buckling fraction decreases with height and is closely coordinated with transfusion tissue capacity and stomatal conductance to provide temporal reserves optimized for local variation in microclimate. Foliar water uptake fully restores collapsed and air-filled transfusion tracheids in leaves on excised shoots, suggesting that trees may use aerial water sources for recovery. In the intensely variable deep-crown environment, foliar water uptake can allow for repetitive cycles of tracheid buckling and unbuckling, protecting the tree from damaging levels of hydraulic tension and supporting leaf survival.

Foliar Water Uptake Capacity in Six Mangrove Species

Foliar water uptake (FWU) is a mechanism that enables plants to acquire water from the atmosphere through their leaves. As mangroves live in a saline sediment water environment, the mechanism of FWU might be of vital importance to acquire freshwater and grow. The goal of this study was to assess the FWU capacity of six different mangrove species belonging to four genera using a series of submersion experiments in which the leaf mass increase was measured and expressed per unit leaf area. The foliar water uptake capacity differed between species with the highest and lowest average water uptake in Avicennia marina (Forssk.) Vierh. (1.52 ± 0.48 mg H2O cm−2) and Bruguiera gymnorhiza (L.) Lam. (0.13 ± 0.06 mg H2O cm−2), respectively. Salt-excreting species showed a higher FWU capacity than non-excreting species. Moreover, A. marina, a salt-excreting species, showed a distinct leaf anatomical trait, i.e., trichomes, which were not observed in the other species and might be involved in the water absorption process. The storage of leaves in moist Ziplock bags prior to measurement caused leaf water uptake to already occur during transport to the field station, which proportionately increased the leaf water potential (A. marina: −0.31 ± 0.13 MPa and B. gymnorhiza: −2.70 ± 0.27 MPa). This increase should be considered when performing best practice leaf water potential measurements but did not affect the quantification of FWU capacity because of the water potential gradient between a leaf and the surrounding water during submersion. Our results highlight the differences that exist in FWU capacity between species residing in the same area and growing under the same environmental conditions. This comparative study therefore enhances our understanding of mangrove species’ functioning.

Foliar water uptake as a source of hydrogen and oxygen in plant biomass.

Introductory biology lessons around the world typically teach that plants absorb water through their roots, but, unfortunately, absorption of water through leaves and subsequent transport and use of this water for biomass formation remains a field limited mostly to specialists. Recent studies have identified foliar water uptake as a significant net water source for terrestrial plants. The growing interest in the development of a new model that includes both foliar water uptake (in liquid form) and root water uptake to explain hydrogen and oxygen isotope ratios in leaf water and tree rings demands a method for distinguishing between these two water sources. Therefore, in this study, I have devised a new labelling method that utilizes two different water sources, one enriched in deuterium (HDO+D2O; δD=7.0×10 4‰, δ18O=4.1‰) and one enriched in oxygen-18 (H218O; δD=-85‰, δ18O=1.1×104‰), to simultaneously label both foliar-absorbed and root-absorbed water and quantify their relative contributions to plant biomass. Using this new method, I here present evidence that, in the case of well-watered Cryptomeria japonica D. Don, hydrogen and oxygen incorporated into new leaf cellulose in the rainy season derives mostly from foliar-absorbed water (69% from foliar-absorbed water and 31% from root-absorbed water), while that of new root cellulose derives mostly from root-absorbed water (20% from foliar-absorbed water and 80% from root-absorbed water), and new branch xylem is somewhere in between (55% from foliar-absorbed water and 45% from root-absorbed water). The dual-labelling method first implemented in this study enables separate and simultaneous labelling of foliar-absorbed and root-absorbed water and offers a new tool to study the uptake, transport and assimilation processes of these waters in terrestrial plants.

Shoot dimorphism enables Sequoia sempervirens to separate requirements for foliar water uptake and photosynthesis.

PremiseTrees in wet forests often have features that prevent water films from covering stomata and inhibiting gas exchange, while many trees in drier environments use foliar water uptake to reduce water stress. In forests with both wet and dry seasons, evergreen trees would benefit from producing leaves capable of balancing rainy‐season photosynthesis with summertime water absorption.MethodsUsing samples collected from across the vertical gradient in tall redwood (Sequoia sempervirens) crowns, we estimated tree‐level foliar water uptake and employed physics‐based causative modeling to identify key functional traits that determine uptake potential by setting hydraulic resistance.ResultsWe showed that Sequoia has two functionally distinct shoot morphotypes. While most shoots specialize in photosynthesis, the axial shoot type is capable of much greater foliar water uptake, and its within‐crown distribution varies with latitude. A suite of leaf surface traits cause hydraulic resistance, leading to variation in uptake capacity among samples.ConclusionsShoot dimorphism gives tall Sequoia trees the capacity to absorb up to 48 kg H2O h−1 during the first hour of leaf wetting, ameliorating water stress while presumably maintaining high photosynthetic capacity year round. Geographic variation in shoot dimorphism suggests that plasticity in shoot‐type distribution and leaf surface traits helps Sequoia maintain a dominate presence in both wet and dry forests.

Leaf surface traits contributing to wettability, water interception and uptake of above-ground water sources in shrubs of Patagonian arid ecosystems.

The ecohydrological significance of leaf wetting due to atmospheric water in arid and semiarid ecosystems is not well understood. In these environments, the inputs of precipitation or dew formation resulting in leaf wetting have positive effects on plant functioning. However, its impact on plant water relations may depend on the degree of leaf surface wettability. In this study we evaluated leaf wettability and other leaf traits and its effects on foliar water uptake and canopy interception in plant species of a Patagonian steppe. We also studied how leaf traits affecting wettability vary seasonally from growing to dry season. Contact angle of a water droplet with the leaf surface, water adhesion, droplet retention angle, stomatal density, cuticular conductance, canopy interception and maximum foliar water uptake were determined in six dominant shrub species. All species increased leaf wettability during the dry season and most species were considered highly wettable. The leaf surface had very high capacity to store and retain water. We found a negative correlation between foliar water uptake and leaf hydrophilia. Despite the diversity of life forms, including cushion shrubs and tall shrubs, as well as phenological variability, all species converged in similar seasonal changes in leaf traits that favour wettability. Intercepted water by crowns and the extremely high capacity of retention of droplets on leaf surfaces can have a significant impact on eco-hydrological process in water limited ecosystems where most of water sources during the growing and the dry season may be small rainfall events or dew, which do not always increase soil water availability.

Foliar water uptake does not contribute to embolism repair in beech (Fagus sylvatica L.).

Foliar water uptake has recently been suggested as a possible mechanism for the restoration of hydraulically dysfunctional xylem vessels. In this paper we used a combination of ecophysiological measurements, X-ray microcomputed tomography and cryo-scanning electron microscopy during a drought treatment to fully evaluate this hypothesis. Based on an assessment of these methods in beech (Fagus sylvatica L.) seedlings we were able to (1) confirm an increase in the amount of hydraulically redistributed water absorbed by leaves when the soil water potential decreased, and (2) locate this redistributed water in hydraulically active vessels in the stem. However, (3) no embolism repair was observed irrespective of the organ under investigation (i.e. stem, petiole or leaf) or the intensity of drought. Our data provide evidence for a hydraulic pathway from the leaf surface to the stem xylem following a water potential gradient, but this pathway exists only in functional vessels and does not play a role in embolism repair for beech.

Foliar water uptake in the needles of Pinus torreyana

The natural habitat of Pinus torreyana, the Torrey pine, is restricted to two locales in coastal Southern California that experience substantial fog and low clouds during the dry months of a Mediterranean climate. In similar semi-arid climate systems that encounter fog or low clouds, many plants can capture atmospheric moisture and are capable of direct foliar water uptake to reduce water stress. In this study, we investigated if the needles of P. torreyana are also capable of direct water uptake. In addition to water immersion, we measured the surface properties along a needle using microdroplets. The droplet contact angle is a measure of surface wettability, and the droplet absorption is a measure of localized foliar water uptake. The results showed that the entire length of the P. torreyana needle, including under the base sheath, is hydrophilic and capable of direct water uptake. The spatial gradients of the wettability and the water uptake along the needle are relatively small. Moreover, the wettability and water uptake increase from young shoots to one-year and two-year old needles. Our results also showed that the decrease in water uptake at higher contact angles can be described by a linear regression. Compared with the leaves of four shrubs in the same habitat, Heteromeles arbutifolia, Malosma laurina, Rhus integrifolia, and Eriodictyon crassifolium, the P. torreyana needles have lower contact angles and higher water uptake rates.

Transcriptome profiling reveals that foliar water uptake occurs with C3 and crassulacean acid metabolism facultative photosynthesis in Tamarix ramosissima under extreme drought.

Tamarix ramosissima is a typical desert plant species that is widely distributed in the desert areas of Northwest China. It plays a significant role in sand fixation and soil water conservation. In particular, how it uses water to survive in the desert plays an important role in plant growth and ecosystem function. Previous studies have revealed that T. ramosissima can alleviate drought by absorbing water from its leaves under extreme drought conditions. To date, there is no clear molecular regulation mechanism to explain foliar water uptake (FWU). In the present study, we correlated diurnal meteorological data, sap flow and photosynthetic parameters to determine the physical and biological characteristics of FWU. Our results suggested that the lesser the groundwater, the easier it is for T. ramosissima to absorb water via the leaves. Gene ontology annotation and Kyoto Encyclopaedia of Genes and Genomes pathway analysis of the transcriptome profile of plants subjected to high humidity suggested that FWU was highly correlated to carbohydrate metabolism, energy transfer, pyruvate metabolism, hormone signal transduction and plant–pathogen interaction. Interestingly, as a C3 plant, genes such as PEPC, PPDK, MDH and RuBP, which are involved in crassulacean acid metabolism (CAM) photosynthesis, were highly upregulated and accompanied by FWU. Therefore, we proposed that in the case of sufficient water supply, C3 photosynthesis is used in T. ramosissima, whereas in cases of extreme drought, starch is degraded to provide CO2 for CAM photosynthesis to make full use of the water obtained via FWU and the water that was transported or stored to assimilating branches and stems. This study may provide not only an important theoretical foundation for FWU and conversion from C3 plants to CAM plants but also for engineering improved photosynthesis in high-yield drought-tolerant plants and mitigation of climate change-driven drought.

Foliar Water Uptake in Eight Mangrove Species: Implications of Morpho-Physiological and Anatomical Traits

Foliar Water Uptake in Eight Mangrove Species: Implications of Morpho-Physiological and Anatomical Traits

Functional Leaf Traits, Foliar Water Uptake and Abundance of Shrubs in Ferruginous Campo Rupestre

Functional Leaf Traits, Foliar Water Uptake and Abundance of Shrubs in Ferruginous Campo Rupestre

Foliar water uptake of four shrub species in a semi-arid desert

Dew water plays an important role in supporting biological processes in desert ecosystems. Although some mechanisms underlying how plants survive in water-limited environments have been investigated, the water-use strategies of most desert plants remain unclear. We investigated whether foliar uptake of dew water occurs in four dominant shrub species (Artemisia ordosica, Hedysarum mongolicum, Salix psammophila, and Caragana korshinskii), in the Mu Us Desert of northern China. A fluorescent tracing experiment indicated that all four species absorbed water via leaf trichomes and cuticles, and A. ordosica also absorbed water via the stomata. A dew water exposure experiment and isotopic tracing revealed that water entered the leaves and stems of the four species, thereby enhancing their water potential. The relative contribution of dew water to the total water in the leaves of A. ordosica, H. mongolicum, S. psammophila, and C. korshinskii was 20.0 ± 7.0%, 8.4 ± 6.4%, 7.8 ± 1.9%, 3.1 ± 2.3%, respectively. The results demonstrated that all four species had the capacity to absorb water by their leaves, and the absorbed water could be transported to the stems. Our findings suggest that foliar water uptake may be one of the survival mechanisms by shrubs in the desert environment.

Cuticular wax composition contributes to different strategies of foliar water uptake in six plant species from foggy rupestrian grassland in tropical mountains

The cuticle is the outermost region of the epidermal cell wall of plant aerial organs. The cuticle acts as a two-way lipid barrier for water diffusion; therefore, it plays a vital role in foliar water uptake (FWU). We hypothesised that the chemical composition of the cuticular waxes influences the FWU strategy that plants adopt in a foggy tropical ecosystem. We analysed the leaf cuticular waxes of six plant species known by their different FWU strategies, in both qualitative and quantitative approaches, to test this hypothesis. We also investigated the fine structure of the plant cuticle by scanning electron microscopy. Neither the total wax loads nor the amounts of single wax compound classes correlated to the FWU. In contrast, the qualitative chemical composition of the cuticular waxes was related to the water absorption speed but not to the maximum water absorbed. The presence of wax crystals might interfere with the FWU. Our findings suggest that a complex three-dimensional network of the cuticular compounds contributes to different strategies of FWU in six plant species from foggy tropical mountaintops.

Desiccation and rehydration dynamics in the epiphytic resurrection fern Pleopeltis polypodioides.

The epiphytic resurrection-or desiccation-tolerant (DT)-fern Pleopeltis polypodioides can survive extreme desiccation and recover physiological activity within hours of rehydration. Yet, how epiphytic DT ferns coordinate between deterioration and recovery of their hydraulic and photosynthetic systems remains poorly understood. We examined the functional status of the leaf vascular system, chlorophyll fluorescence, and photosynthetic rate during desiccation and rehydration of P. polypodioides. Xylem tracheids in the stipe embolized within 3-4 h during dehydration. When the leaf and rhizome received water, tracheids refilled after ∼24 h, which occurred along with dramatic structural changes in the stele. Photosynthetic rate and chlorophyll fluorescence recovered to predesiccation values within 12 h of rehydration, regardless of whether fronds were connected to their rhizome. Our data show that the epiphytic DT fern P. polypodioides can utilize foliar water uptake to rehydrate the leaf mesophyll and recover photosynthesis despite a broken hydraulic connection to the rhizome.

Foliar water uptake via cork warts in mangroves of the Sonneratia genus.

Foliar water uptake (FWU) occurs in plants of diverse ecosystems; however, the diversity of pathways and their associated FWU kinetics remain poorly resolved. We characterized a novel FWU pathway in two mangrove species of the Sonneratia genus, S. alba and S. caseolaris. Further, we assessed the influence of leaf wetting duration, wet-dry seasonality and leaf dehydration on leaf conductance to surface water (Ksurf ). The symplastic tracer dye, disodium fluorescein, revealed living cells subtending and encircling leaf epidermal structures known as cork warts as a pathway of FWU entry into the leaf. Rehydration kinetics experiments revealed a novel mode of FWU, with slow and steady rates of water uptake persistent over a duration of 12 hr. Ksurf increased with longer durations of leaf wetting and was greater in leaves with more negative water potentials at the initiation of leaf wetting. Ksurf declined by 68% between wet and dry seasons. Our results suggest that FWU via cork warts in Sonneratia sp. may be rate limited and under active regulation. We conclude that FWU pathways in halophytes may require ion exclusion to avoid uptake of salt when inundated, paralleling the capacity of halophyte roots for ion selectivity during water acquisition.

Leaf trichomes of Dendrobium species (epiphytic orchids) in relation to foliar water uptake, leaf surface wettability, and water balance

Vascular epiphytes play an important role in the water and carbon cycles of forest ecosystems. While trichomes are found on the leaf surface of many epiphytic species, few studies have investigated the effect of leaf trichomes on their foliar water uptake, leaf surface wettability, and water balance. In our study, leaf water absorption, storage, and retention capacities, as well as leaf surface wettability of twelve Dendrobium species with glabrous (n = 6) and piliferous (n = 6) leaves were investigated and related to their leaf morphological and anatomical characteristics. Our results showed that no significant difference in foliar water uptake capacity was found between groups with different leaf surface types (with and without trichomes). However, the piliferous Dendrobium species showed a significantly larger increase in leaf water content (%LWC) and lower values for both saturated water content per unit mass and water retention capacity compared to glabrous ones. Across Dendrobium species with piliferous leaves, the trichome density on leaf abaxial surface was positively correlated with foliar water uptake capacity per unit area, contact angle, stomatal density and stomata area per unit index. Besides, species with a higher %LWC after leaf immersion dried faster from saturated leaves to a relative water content of 70 %, which suggests a potential trade-off between leaf water absorption and conservation in epiphytic Dendrobium species. The variation in leaf water absorption and conservation suggests that different water use strategies are utilized by epiphytic orchids with glabrous and piliferous leaves.

Functional assembly of tropical montane tree islands in the Atlantic Forest is shaped by stress tolerance, bamboo presence, and facilitation.

AimsAmidst the Campos de Altitude (Highland Grasslands) in the Brazilian Atlantic Forest, woody communities grow either clustered in tree islands or interspersed within the herbaceous matrix. The functional ecology, diversity, and biotic processes shaping these plant communities are largely unstudied. We characterized the functional assembly and diversity of these tropical montane woody communities and investigated how they fit within Grime's CSR (C—competitor, S—stress‐tolerant, R—ruderal) scheme, what functional trade‐offs they exhibit, and how traits and functional diversity vary in response to bamboo presence/absence.MethodsTo characterize the functional composition of the community, we sampled five leaf traits and wood density along transects covering the woody communities both inside tree islands and outside (i.e., isolated woody plants in the grasslands community). Then, we used Mann–Whitney test, t test, and variation partitioning to determine the effects of inside versus outside tree island and bamboo presence on community‐weighted means, woody species diversity, and functional diversity.ResultsWe found a general SC/S strategy with drought‐related functional trade‐offs. Woody plants in tree islands had more acquisitive traits than those within the grasslands. Trait variation was mostly taxonomically than spatially driven, and species composition varied between inside and outside tree islands. Leaf thickness, wood density, and foliar water uptake were unrelated to CSR strategies, suggesting independent trait dimensions and multiple drought‐coping strategies within the predominant S strategy. Islands with bamboo presence showed lower Simpson diversity, lower functional dispersion, lower foliar water uptake, and greater leaf thickness than in tree islands without bamboo.ConclusionsThe observed functional assembly hints toward large‐scale environmental abiotic filtering shaping a stress‐tolerant community strategy, and small‐scale biotic interactions driving small‐scale trait variation. We recommend experimental studies with fire, facilitation treatments, ecophysiological and recruitment traits to elucidate on future tree island expansion and community response to climate change.

Studies on foliar uptake of tritiated water on Spinach sp. during light and dark simulated conditions using environmental chamber

Studies on foliar uptake of tritiated water (HTO) on Spinach sp. during light and dark condition was simulated inside the environmental chamber, and an attempt was made to compute CTFWT HTO, Cpfw HTO, Cpcw OBT, Cpfw OBT using basic equations available in IAEA TECDOC1616. The predicted activity was compared with the observed activity. The conversion of tissue-free water tritium (TFWT) to organically bound tritium (OBT) was more in the light condition. The ratio of OBT to TFWT varied from 0.006 to 0.082. The ratio of observed OBT activity (Bq/kg fresh weight) in light to dark conditions varied from 34% to 117%.

Evidence of foliar water uptake in a conifer species

Foliar water uptake (FWU) is the main mechanism used by herbaceous plants in arid areas to absorb small amounts of precipitation. It has an important ecological and hydrological effect for alleviating plant drought stress. However, it is unknown whether woody plants can absorb water through the leaves when water absorption via roots is insufficient. This study explored whether FWU exists and the FWU conditions in semi-humid rock mountainous areas. Isotopes were labeled to detect FWU under different soil water content (SWC) gradients (3.9–6.5%, 6.5–9.1%, 9.1–15.6%, 15.6–20.8%, and 20.8–26.0%) and different precipitation gradients (1 mm/h, 5 mm/h, 10 mm/h, and 15 mm/h) in simulated precipitation experiments with indoor potted plants. The results showed that FWU occurred in each treatment if the SWC ≤ 21.9% no matter the precipitation amount. Water absorption via FWU increased with the increase of precipitation intensity but decreased with the increase of SWC. The greatest ratios of FWU were 2.77% and 9.52% of precipitation intensity of 1 mm/h and 15 mm/h, respectively, in the 3.9–6.5% treatment. The precipitation absorbed by the leaves can be transported to the xylem or root system along the water potential gradient of leaves–branches–roots. The precipitation with reverse migration in branches and roots increased with the increase of the water potential gradient of leaves–branches–roots. These findings suggest that Platycladus orientalis can uptake water through roots and leaves, effectively alleviating drought stress. This study provides new insights into water use patterns and water migration in trees.

Photosynthetic heat tolerance in plants with different foliar water -uptake strategies.

The distribution and even the survival of plant species are influenced by temperature. In an old climatically buffered infertile landscape (OCBIL) in Brazil, we previously characterized different strategies for foliar water uptake (FWU). It is possible that photosystem II tolerance to heat and excessive light intensity varies among species with different FWU capacities. The relationship between FWU, photoinhibition, and thermotolerance was investigated in seven species from this ecosystem. The species with slow water absorption and high water absorption are those that presented less photoinhibition. Contrastingly, the species that have fast and low water absorption presented greater thermotolerance when their leaves are totally hydrated. However, when there is greater leaf dehydration, the most thermotolerant species were those with slow but high water absorption. Foliar water uptake is an important trait for plants to tolerate excessive light intensity and higher temperatures. Plants in this OCBIL may be differentially affected by future global warming, and the best strategy to deal with this expected climate change is with slow and high absorption of water.