Boron (B) is often deficient in many fruit crops, including blueberry (Vaccinium sp.). The objective of the present study was to evaluate different methods for applying B fertilizers to two commercial cultivars of northern highbush blueberry (V. corymbosum Earliblue and Aurora) in western Oregon, USA. Treatments included soil application of sodium tetraborate in early April (before bloom), foliar application of boric acid in late April (during bloom or petal fall), weekly fertigation with boric acid from April through July, and a control with no B. The plants were irrigated by drip, and the fertilizers were applied for two consecutive seasons at a total rate of 1.5 kg·ha−1 B per year. Each method of fertilizer application increased the concentration of B in the soil solution relative to the control, but fertigation was the only treatment that increased extractable soil B to the recommended level of 0.5 to 1.0 mg·kg−1 B. In terms of plant nutrition, foliar application of B was the most effective method for increasing the concentration of B in the leaves, roots, and fruit, followed by fertigation. Soil application of B, on the other hand, was relatively ineffective and, after 2 years, only increased the concentration of B in the leaves of ‘Earliblue’. Although leaf B levels were initially deficient at the site (<30 ppm B), none of the B application methods had any effect on yield, berry weight, fruit firmness, or titratable acidity of the fruit in either cultivar. However, foliar applied B resulted in higher concentrations of soluble solids in the fruit than no B or soil applied B in ‘Earliblue’, whereas B fertigation resulted in higher concentrations of soluble solids than soil applied B in ‘Aurora’. On the basis of these results, applying B by fertigation or as a foliar spray is recommended over the use of soil applications of B fertilizer in northern highbush blueberry.