Foliar Application Of Ascorbic Acid Research Articles

Foliar Application of Ascorbic Acid and Gamma Aminobutyric Acid Can Improve Important Properties of Deficit Irrigated Cucumber Plants (Cucumis sativus cv. Us)

Cucumber and other Cucurbitaceae plants have a high transpiration rate and sensitivity to water deficit stress. Ascorbic acid (AsA) and gamma aminobutyric acid (GABA) participate in a variety of plant processes and increase plant resistance to different stresses. The current study was aimed to assess the efficacy of the foliar application of AsA (100, 200 and 300 mg l−1) and GABA (20 and 40 mM) on growth, physiological parameters and water use efficiency related properties of cucumber plants under deficit irrigation (DI) strategy (0, 25 and 50%). Results showed that the highest values of measured morphological properties were recorded in plants treated with GABA at 40 mM and AsA at 100 mg l−1. The highest water use efficiency was observed in plants sprayed with GABA at 40 mM. Also, foliar spraying of GABA at 40 mM was the best treatment to improve the free proline content of leaves and the activity of antioxidant enzymes under drought stress conditions. Findings of this study showed that foliar application of AsA and GABA can be a useful and inexpensive treatment to improve morphological, physiological and water use efficiency related properties of cucumber plant under water deficit stress.

Improvement of drought tolerance in five different cultivars of Vicia faba with foliar application of ascorbic acid or silicon

Aim of study: To explore the role of ascorbic acid (AsA) or silicon (Si) in improving drought tolerance in five faba bean cultivars under irrigation water deficit (IWD).Area of study: The experimental farm; 30° 36′ N, 32° 16′ E, Egypt.Material and methods: Three drip irrigation regimes (WW, well-watered, 4000 m3 water ha-1; MD, moderate drought, 3000 m3 water ha-1; and SD, severe drought, 2000 m3 water ha-1) were applied to plants, which were sprayed 25, 40, and 55 days after sowing with 1.5 mM AsA or 2.0 mM Si vs distilled water as a control.Main results: Drought negatively affected physiological attributes (photosynthetic pigments, gas exchange parameters, relative water content, membrane stability index, electrolyte leakage (EL), and lipid peroxidation), which restricted plant growth and yields, and stimulated alterations in both enzymatic and non-enzymatic antioxidant activities. However, AsA or Si application mitigated drought effects on physiological attributes, improving growth, yields and water use efficiency by raising antioxidant activities and suppressing lipid peroxidation and EL in stressful cultivars. The mitigating effects of AsA and Si were more pronounced under MD.Research highlights: ‘Nubaria-2’, ‘Giza-843’, and ‘Sakha-3’ were more tolerant than ‘Giza-716’ and ‘Sakha-4’, suggesting the use of AsA or Si to ameliorate the IWD effects on stressful cultivars. Certain physiological traits exhibited positive association with growth and seed yield, demonstrating their importance in enhancing seed yield under irrigation treatments.

Impact of Ascorbic acid and Potassium on Okra (Abelmoschus esculentus) Growth in Saline Condition

                 Salinity regards as one of the abiotic stress factors affect that effect the growth and development of the plant. In present investigation the combination effect of ascorbic acid and potassium has been studies on okra growth under salt stress condition. The study conducted in factorial experiment of three replications in Completely Randomized Designed replicated three times. Three levels of potassium (K) (0,200, 400 ppm) applied to the soil. Three levels of foliar application of ascorbic acid (ASA) (0, 100, 200ppm). Irrigated with three levels of NaCl (0,100,200 µS). Parameters were recorded plant height (cm), number of leaves per plant, leaf area (cm2), water content (%), chlorophyll content, protein and proline content of leaves, mineral content; nitrogen, phosphorus, calcium, potassium, and sodium.  The results elucidate that combination impact of ascorbic acid and potassium significantly the tolerance of okra plant to salinity due to an increase in height of plant, number of leaves, shoot water content, protein and proline content of leaves, and mineral contents such as nitrogen and sodium content of leaves under salt stress conditions.

Effect of foliar application of ascorbic acid on sweet pepper (Capsicum annuum) plants under drought stress

Drought is a harmful abiotic factor that clearly affects the growth and production of plants. The negative impacts of drought can be reduced by ascorbic acid (AsA) which is an important non-enzymatic antioxidant in plants. The purpose of the current investigation was to study the effects of different levels of drought and the external application of AsA on sweet pepper plants. The experiment had two factors, the first factor was drought: irrigation within the field capacity, moderate stress (irrigation within the 60% field capacity) and severe stress (irrigation within the 30% field capacity). The second factor was AsA: 0 mM sprayed with distilled water, 0.5 and 1 mM. The experiment had three replications. The results illustrated that drought stress reduced the fresh and dry weight of roots and shoots. It also reduced the relative water content (RWC), diameter and length of fruit, chlorophyll content and leaf area. Drought stress increased the activity of polyphenol oxidase and ascorbate peroxidase enzymes. It caused improvements in antioxidant properties, total phenolic content, ascorbate and electrical conductivity (EC) in the leaves of sweet pepper. Higher concentrations of AsA by foliar application significantly increased the values of all measured parameters except the EC. The interaction between ascorbic acid and drought stress showed that ascorbic acid significantly increased the shoot fresh weight, root dry weight, antioxidant properties, ascorbate, polyphenol oxidase and ascorbate peroxidase. However, it reduced the electrical conductivity. It is claimed that sweet pepper plants acquire tolerance to drought because AsA contributes to an increase in antioxidant properties.

Effect of foliar application of acetyl salicylic acid, ascorbic acid and riboflavin on growth, yield and yield component of French bean (Phaseolus vulgaris L Cv. Arka Arjun)

The present experiment was carried out toassess the influence of foliar application of antioxidants on growth, yield and yield component of French bean cv. Arka Arjun. This experiment consisted of 7 treatment viz. Distilled water spray (T1), Acetyl Salicylic Acid @ 100ppm (T2), Acetyl Salicylic Acid @ 200 ppm (T3), Ascorbic Acid @ 100 ppm (T4), Ascorbic Acid @ 200ppm (T5), Riboflavin @ 200 ppm (T6) and Riboflavin @ 400 ppm (T7). Five plants in each plot were randomly selected for recording the growth, yield observation and its parameters. Data was collected on different traits such as Plant height, Number of branches per plant, Number of leaves per plant, Leaf Area Index, Number of pods per plant, Pod weight of ten fruits, Pod length, Pod diameter, Pod yield per plant, Protein content of pod. The salient findings of the present investigation have been summarized as under: such as Plant height was significantly influenced by various treatments The maximum Plant height was recorded in the treatment T5 (Ascorbic Acid @ 200ppm). Maximum number of branches per plant was observed for treatment T5 (Ascorbic Acid @ 200ppm). The maximum number of leaves per plant was recorded in the treatment T5 (Ascorbic Acid @ 200ppm). Maximum Leaf Area Index was recorded in the treatment T5 (Ascorbic Acid @ 200ppm). The maximum number of pods per plant was recorded in the treatment T3 (ASA @ 200ppm) Maximum pod weight of ten fruits was recorded in the treatment T5 (Ascorbic acid @ 200ppm). The maximum fruit length was recorded in the treatment T5 (Ascorbic acid @ 200ppm). Highest Pod yield per plant was recorded in the treatment T5 (Ascorbic acid @ 200ppm) also. Maximum value of protein content was observed for treatment T3 (ASA @ 200ppm). These results prove that foliar applications of Ascorbic Acid at concentrations of 200 ppm (T5) were beneficial for the growth and development of French bean. Maximum yield per plant was recorded with the treatment application of Ascorbic acid at concentrations of 200 ppm (T5) and was found to be superior over Acetyl Salicylic Acid concentrations. Maximum protein content was recorded with the treatment application of Acetyl Salicylic Acid at concentrations 200 ppm (T3) and was found to be superior over Ascorbic Acid concentrations.

Influence of foliar application of ascorbic acid and atonik on growth and yield of potato

Influence of foliar application of ascorbic acid and atonik on growth and yield of potato

Pre-treatment of two contrasting water-stressed genotypes of cassava (Manihot esculenta Crantz) with ascorbic acid. I. Growth, physiological and antioxidant responses.

Water deficit-stress at early growth stage is a major constraint of cassava production. Ascorbic acid is a non-enzymatic antioxidant that mitigates oxidative damage caused by water stress in plants. Growth, physiological and antioxidant defense system responses of two contrasting water-stressed cassava genotypes to pre-treatment with foliar application of ascorbic acid (AA) were investigated. The objectives of this study were to assess the growth, proline, photosynthesis pigments and antioxidant activities of young water-stressed cassava plants pre-treated with ascorbic acid. The study consisted of IITA-TMS-IBA980581 (drought tolerant) and IITA-TMS-IBA010040 (drought sensitive) cassava genotypes treated with six doses (0.00, 0.25, 0.50, 0.75 and 1.00mM) of AA before beingsubjected to water deficit (45.0% field capacity) and a water sufficient AA-untreated control. In both genotypes, water stress reduced shoot height (40.3%), leaf area (42.5%), and number of root (54.5%), biomass (28.6%), relative water content (RWC, 3.2%) and photosynthetic pigments (300.0%). However, water stress increased proline (91.3%), endogenous AA (112.0%), catalase (CAT, 300.0%) and superoxide dismutase (SOD, 15.3%) in both genotypes. Compared with IITA-TMS-IBA010040, leaf area, biomass, number of root and shoot height of IITA-TMS-IBA980581 were higher by 7.3, 24.6, 25.9 and 13.1%, respectively. By less than a quarter, chlorophylls a and b, activity of superoxide dismutase and relative water content of IITA-TMS-IBA980581 were higher compared with IITA-TMS-IBA010040. However, proline content of IITA-TMS-IBA010040 was higher than IITA-TMS-IBA980581 by 14.3%. Pre-treatment with AA improved growth parameters, photosynthetic pigments, RWC, endogenous AA, activity of CAT and SOD, but decreased proline in both genotypes with an optimum concentration at 0.5mM. Pre-treatment with 0.5mM AA increased shoot height, area of leaves, leaf number, number of root and dry weight by 46.3, 44.7, 14.4, 88.2 and 37.5%, respectively. Pre-treatment with 0.5mM AA doubled chlorophylls, tripled carotenoids content, doubled endogenous AA and slightly enhanced RWC (2.1%) and SOD (2.0%) when compared with AA-untreated water stressed plants. But pre-stress application of AA reduced proline content by one-fold, increased CAT activity by one-fold in IITA-TMS-IBA980581 and by one-third in IITA-TMS-IBA010040. The study concluded that pre-treatment of cassava young plants with AA before water deficit could alleviate oxidative stress.

Effect of Ascorbic, Folic acids and Hibiscus Extract on Geranium (<i>Pelargonium graveolens</i>)

The present investigation was carried out in Sabahia Horticulture Research Station, Alexandria, Egypt during 2015/2016 - 2016/2017 seasons to study the effect of foliar application with ascorbic acid, folic acid as well as hibiscus extract on quality of Geranium (Pelargonium graveolens L.). Foliar application of ascorbic acid and folic acid as well as extract of hibiscus were sprayed twice in each cut (first spry before cut 60 days and second spray before cut 30 days). Treatments included: control water only, ascorbic acid at (100 and 200 ppm), folic acid at (50 and 100ppm) and hibiscus extract at (5% and 10%). The essential oil (%) of Geranium was determined by hydro-distillation as well as the main constituents of the volatile oil were determined by (GC). The Results showed that, all treatments (tested) caused a great increase in vegetative growth, the essential oil (%), oil yield (ml/plant and L/Fed.) and active compounds; Citronerlol, Citronelly formate, Iso-menthone, B-caryophyllene and Geranyl formate, while it decreased α-pinene and Geraniol. In addition, ascorbic acid at 200ppm, folic acid at 100ppm and hibiscus extract at (5, 10%) are sharp efficacy on increasing the vegetative growth and volatile oil yield than other treatments. Furthermore, it could be illustrated that natural drying caused pronounced changes (acceptable) in the volatile oil% and main constituents.

Agronomic performance and gaseous exchanges of the radish under saline stress and ascorbic acid application

The radish is a short-cycle vegetable that has excellent nutritional and medicinal properties. It is considered rustic, meaning it tolerates adverse conditions with the possibility of being irrigated with saline water, which creates stress. In this context, this study aimed to evaluate the effect of electrical conductivities in irrigation water and doses of ascorbic acid on the agronomic performance and gaseous exchanges of radishes. This experiment was carried out in a protected environment at the Agricultural Sciences Center of the Federal University of Paraíba, Areia, Paraíba (Brazil). The experiment design used randomized blocks with five doses of ascorbic acid (0.0, 0.29, 1.0, 1.71 and 2.0 mM) and five electrical conductivities in the irrigation water (0.5, 1.3, 3.25, 5.2 and 6.0 dS m-1), with four replicates. The growth, gas exchange and production were evaluated. The doses of ascorbic acid were not significant. The increase in the electrical conductivities of the irrigation water provided a reduction in the agronomic performance and gas exchanges, except for the net photosynthesis, water use efficiency and instantaneous carboxylation efficiency, which were not significant. There was a relationship between the net photosynthesis, transpiration, internal concentration of CO2 and water use efficiency and the stomatal conductance. The agronomic performance and gaseous exchanges of the radish culture were influenced by the salinity. The foliar application of ascorbic acid did not influence the agronomic yield and gaseous exchanges of the radishes at the tested doses.

Foliar Application of Ascorbic Acid Combined with Nitrogenous Fertilizer Positively Enhanced the Response of Pea Plants

This study was conducted on pea (Pisum sativum L.) cv. Master B in the Experimental Farm of Vegetable Research Station, Faculty of Agriculture, Mansoura University, Egypt during two winter seasons of 2015/2016 and 2016/2017 under silty clay loam conditions by using furrow irrigation system to investigate the effect of ascorbic acid (AsA), inorganic nitrogen (N) fertilization and their interactions on growth, productivity and quality of pea. Therefore, four concentrations of AsA, viz, 0, 100, 200 and 400 mg L-1 (ppm) applied as a foliar application combined with three N-fertilization levels, i.e., 60, 80 and 100 kg fed.-1 as a soil application were studied. The experiment was laid out as split plot in randomized complete block design (RCBD) with three replications. The obtained results revealed that fertilization of pea plants with 100 kg N/fed. combined with foliar application of AsA at 400 mg L-1 followed by 80 kg N/fed. with 400 mg L-1 of AsA gave significant increases for the traits of plant vegetative growth (plant height and fresh weight, branches and leaves number/plant, leaves fresh weight, leaves area/plant and partially leaves dry matter percentage), leaves chemical composition (N, P and K percentages and chlorophyll a, b and carotenoids contents), pods and seeds yield components (number and fresh weight of green pods/plant, green pods yield/fed., 100 seeds fresh weight and green seeds yield/fed.), seeds quality (Vit. C, TSS, total carbohydrates, crude protein, N, P, and K contents) and moderate values for both seeds nitrate (NO3) and dry matter percentage of pea compared with control treatment (60 kg N/fed. only), without significant differences between two interaction treatments in both seasons. Concerning the effect of nitrogen fertilization levels, all above mentioned parameters significantly increased with increasing N-fertilization level from 60 kg /fed. to 100 kg /fed. and vice versa for leaves and seeds dry matter percentages and seeds nitrate (NO3) content in both seasons. As for the impact of AsA, the rate of 400 ppm recorded significant increases for all forecited parameters with the lowest nitrate content of dry seeds compared with control treatment (untreated plants), with no significant differences among AsA concentrations in this respect in both seasons. Therefore, adding 100 kg N/fed. combined with AsA at 400 mg L-1 as a foliar application was the best treatment of which this study recommends to use followed by the treatment of 80 kg N/fed. + 400 mg L-1 of AsA to reduce the costs of production, environmental pollution and produce safe food for human health.

Foliar Application of Organic Compounds Stimulate Cotton (Gossypium barbadense L.) to Survive Late Sown Condition

Abstract Application of organic compounds in cotton balances its vegetative and reproductive growth, as well as to improve the seed cotton yield and lint quality properties. Field experiments were conducted with some organic compounds to determine their effects on cotton yield and quality properties of cotton under two sowing times.The experiment was conducted at Sakha Agricultural Research Station, Cotton Research Institute, Egypt in 2015 and 2016. In these studies, two sowing times viz. optimum and delay sowing and two levels (200 and 400 ppm) of organic compounds viz. ascorbic, ascobine and salicylic acids were used. The results indicated that the optimum sowing date gave the largest area of single leaf at 90 and 120 DAS, as well as it induced an increase in dry weight (g) of plant organs at 120 DAS, while, the late sowing significantly reduced the leaves dry weight both at 90 and 120 DAS. Both seed cotton yield and lint cotton yield (kentar feddan-1) were significantly increased in optimum sowing condition and sharply declined as delayed sowing date. The number of opened bolls plant-1 were also significantly reduced in late sowing. Lint percentage, seed index and seed cotton weight boll-1 were also significantly affected by sowing dates. Leaf pigment (total chlorophyll) was significantly affected by sowing date recorded at 90 and 120 DAS, while lower values of total chlorophyll was recorded in late sown plants. Also, sowing dates had no significant effect on both oil and protein seed content. This study has shown that timely sowing of cotton seed and foliar application of ascorbic acid, ascobine or salicylic acid with concentration of 200 or 400 ppm at flower initiation stage and peak of flowering stage had significant positive effects on seed cotton yield and lint quality properties. According to these results it can be suggested that usage of organic compounds to improve the yield and quality properties, especially in late sowing for ‘Giza 94' cotton cultivar at Kafr- Elsheikh district, Egypt.

RESPONSE OF SESAME TO FOLIAR SPRAY WITH ASCORBIC ACID UNDER WATER STRESS CONDITION.

A trail was conducted in wiry greenhouse of Crop PhysiologyDepartment at Giza Experimental Research Station, ARC, during the twosummer seasons of 2014 and 2015 study the alleviation of water deficitstress effects on sesame plant (cv. Shandaweel. 3) by foliar application ofascorbic acid (A.A.) i.e. control, 200 and 400 ppm. Different levels ofwater were applied i.e. 2600 m3/fad (wet), 2300 m3/fad (moist), 2000m3/fad (medium) and 1700 m3/fad (dry). Results could be summarized asfollows:- The highest values of plant height, fruiting zone length, leaf area/plant,capsules number and weight/plant, 1000 seed weight were scored fromirrigation by 2600 m3/fad followed by 2300 m3/fad with insignificantdifference between such two treatments. Whereas, the highest values ofshoot dry weight/plant as well as straw and seed yields were obtainedwhen plants irrigated by 2300 m3/fad followed by 2600 m3/fad. While, thelowest values of all growth traits, yield and yield components wereobtained when plants irrigated by 1700 m3/fad. Protein content of seedincreased and oil content decreased when plants irrigated by 1700 m3/fad(dry). Relative water content (RWC%) decreased gradually withdecreasing water applied up to irrigation by 1700 m3/fad. Waterconsumptive use (WCU) increased with increasing water applied from1700 to 2600 m3/fad. The heights value of water use efficiency (WUE)was recorded when plants irrigated by 2300 m3/fad.- The foliar application of 400 ppm ascorbic acid significantly increasedplant height, fruiting zone length, leaf area/plant, shoot dry weight,capsules number and weight/plant, 1000-seed weight as well as straw andseed yields, protein and oil contents of seeds, RWC, WCU and WUEcompared to control and 200 ppm ascorbic acid.-The interaction effect recorded the maximum values of leaf area / plant,capsules number and weight / plant, oil content of seeds, RWC and WUEwhen plants irrigated by 2600 m3/fad and sprayed with 400 ppm ascorbicacid. Whereas, the highest values of shoot dry weight/plant, straw andseed yields as well as WUE were recorded when plants irrigated by 2300m3/fad. and sprayed with 400 ppm ascorbic acid. The maximum value ofseeds protein content was obtained when plants irrigated by 1700 m3/fadand sprayed by 400 ppm ascorbic acid.

Foliar application of ascorbic and citric acids enhanced 'Red Spur' apple fruit quality, bioactive compounds and antioxidant activity.

This study aimed to assess the effect of the foliar application of ascorbic acid (AA) and citric acid (CA) on total antioxidant activity (TAA), total phenolics, total flavonoids, total anthocyanin content, antioxidant enzymes, phenylalanine ammonialyase (PAL), and polyphenol oxidase (PPO) activities in apple 'Red Spur'. The experiment was conducted on 12-years-old trees 'Red Spur' grafted on MM106 rootstock. The trees were sprayed with AA (0, 200 and 400mgL-1) and/or CA (0, 200 and 400mgL-1) at three different times during summer. Foliar application with AA and CA significantly (p<0.01) enhanced all measured quality attributes and decreased the activity of PPO. Fruit from trees treated with AA at 400mgL-1 and CA at 200mgL-1 showed the highest TAA and catalase (CAT) enzyme activity. Total phenolics increased in fruits when trees were sprayed with AA and CA. Contrasting, AA treatment, CA had no significant effect on guaiacol peroxidase (G-POD). A significant decrease in PPO activity was detected in fruits when treated with both AA and CA. Both treatments significantly decreased the activity of PAL at 400mgL-1. Considering the results, foliar application of AA and CA, either alone or in combination improved the quality and nutraceutical properties of 'Red Spur' apple.

Integrative application of cyanobacteria and antioxidants improves common bean performance under saline conditions

Abstract Cyanobacteria (CB), known as blue-green algae, are useful microscopic organisms that can lead to improve the nutrient uptake, plant growth, and plant tolerance to abiotic stress such as salinity. The impacts of CB, with or without glutathione (GSH) and ascorbic acid (AsA) and their combinations on morphological, physiological, metabolic, and enzymatic activity status in common bean plant grown on salt stressed soil were investigated. All single (CB, AsA or GSH), or combined (CB + AsA, CB + GSH, CB + AsA + GSH or CB + GSH + AsA) applications significantly increased plant length, number and area of leaves, plant fresh and dry weights, yield parameters (green pods weight per plant, dry seed weight per plant and 100-seed weight) and leaf photosynthetic pigments and their photochemical efficiency (Fv/Fm and PI) of common bean plants compared to the control in 2015 and 2016 growing seasons. In addition, relative water content, membrane stability index, contents of soluble sugar, proline, AsA, GSH, N, P and K+ ion contents, and activities of superoxide dismutase, catalase and guaiacol peroxidase were significantly increased with all of the mentioned applications. In contrast, electrolyte leakage and Na+ ion content was significantly decreased. The best response was obtained with the integrative CB + AsA + GSH and CB + GSH + AsA treatments, with distinguish of the former. Overall, these results suggest that supporting the seed CB application with foliar application of AsA and GSH helped to increase the defense systems of the common bean plant to tolerate the adverse effects of soil salinity.

Alleviation Harmful Impacts of Salinity Using Some Antioxidants Substances on Thompson Seedless Grapevines Seedlings

This work was conducted during two successive growing seasons of 2016 and 2017 in the shade house of the Viticulture Research Department, Horticulture Research Institute, Agricultural Research Center, Giza, Egypt, to evaluate the effect of foliar application of ascorbic acid at 400 ppm and salicylic acid at 200 ppm on vegetative growth, physiological characters and endogenous bio constituents, as well as anatomical structure of the leaf blade of the Thompson seedless grape rootings grown under three levels of salinity stress (1000, 2000 and 3000 ppm). The results indicated that, all parameters studied of vegetative growth of rootings significantly decreased by increasing salinity stress levels,in addition to significant decrease in photosynthetic pigments content comparison with untreated vines.In contrast, salinity stress significantlyincreasedproline, sodium, total phenols compoundand oxidative enzymes activity in leaves. Foliar spray with ascorbic acid (AA) gave the best results followed by salicylic acid (SA) to alleviate and overcome the harmful impacts of salinity stress on the aforementioned characters of both growing seasons compared to control vines. Moreover, irrigated plants with NaCl saline solution had considerable decrease in anatomical characters of Thompson seedless grape leaf. Treating rootings with ascorbic acid application at 400 ppm increased all anatomical features in comparison with control vines under salinity stress condition.

Herbicide isoproturon aggravates the damage of low temperature stress and exogenous ascorbic acid alleviates the combined stress in wheat seedlings

Herbicides are largely used to control weed growth in wheat production. However, the application of some kind of herbicides usually aggravates the damage caused by low temperature, and is also a very common adversity during the early growth stages of wheat. In this study, we pre-treated wheat seedlings with the herbicide chemicals of isoproturon, fluroxypyr and fenoxaprop-P-ethyl, respectively and then exposed the plants to low temperature stress with different time intervals. The herbicides, especially isoproturon, significantly increased the negative effects of low temperature on electron transport rate, chlorophyll content, cell membrane stability as well as growth of wheat plants. Furthermore, the foliar application of ascorbic acid (AsA) decreased relative electric conductivity, reduced the content of malondialdehyde, and reduced the production rate of reactive oxygen species, these were in line with elevated activities of superoxide dismutase, catalase and peroxidase under the combined stress of isoproturon and low temperature, indicating that AsA effectively mitigated the sever oxidative stress induced by the combined stresses. Thus, some of the herbicides such as isoproturon are suggested to be very carefully used before the possibility of low temperature events. In case low temperature stress occurs after using of isoproturon, AsA could be used to partially alleviate the damage by the combined stress of isoproturon and low temperature in wheat production.

Anti-Oxidant Activities, Chemical Attributes and Fruit Yield of Peach Cultivars as Influenced by Foliar Application of Ascorbic Acid

Peach fruit is enriched with natural antioxidants but oxidation caused by biotic and abiotic stresses, reduce these antioxidants and consequently effect the quality and yield of the fruit. Therefore, an experiment was conducted to investigate the role of ascorbic acid to sustain the natural antioxidant activity, improve the fruit quality and yield of peach cultivars (Early Grand and Florida King). The peach cultivars, Early Grand and Florida King were tested against four levels of ascorbic acid (200, 400, 600 and 800 ppm) and compared with control as water spray. The foliar application of ascorbic acid significantly influenced anti-oxidant activity and physico-chemical attributes of peach cultivars. However, the foliar application of ascorbic acid @ 800 ppm significantly increased the fruit weight, fruit yield, fruit firmness, total soluble solids, titratable acidity, antioxidant activity of fruit peel, with least percent infected fruits and fruit juice pH as compared to the rest of the treatments. Similarly, significant variation was recorded for peach cultivars regarding anti-oxidant activity and various physico-chemical attributes. The maximum fruit weight, fruit yield, fruit firmness, total soluble solids, titratable acidity and TSS/TA ratio were noted in fruit of cultivar Florida King. On other side the foliar treated fruit of peach cultivar Early Grand had the highest percent infected fruits, fruit juice pH and antioxidant activity of fruit peel. It is concluded from the significant findings of present research that the peach cultivar Florida King performed better in terms of maximum fruit yield, whereas the cultivar Early Grand produced better quality fruits with high anti-oxidant activity when treated with 800 ppm of ascorbic acid as foliar spray.

Effect of organic manure,antioxidant and proline on corn(Zea mays L.)grown under saline conditions.

ABSTRACT Two field experiment using maize were conducted at the Experimental Station of the Agriculture Research Center in Tag El-Ezz, Dakahlia Governorate, Egypt, during the growing seasons of 2016 and 2017. Eighteen treatments were arranged in split-split block design , which were the simple possible combination between three sources of organic manures (control, FYM and chicken manure) as main plots and three sources of antioxidants in foliar way (control, ascorbic acid and salicylic acid) as sub plots and two levels of proline as foliar application (without, 50 ppm) as sub-sub plots. . The results indicated that, the highest mean values of vegetative growth (plant height, fresh, dry weight of flag), chemical content of flag leaf (chlorophyll a, b, total, proline), quality (N, P and K % in grain, straw and cobs, fat, ash and protein%), yield and its components (cob length, no. of grain/cob, 100 grain weight, grain yield ton/fed, straw yield ton/fed and cobs yield ton/fed of corn) recorded with using chicken manure . The highest mean values of all parameters under investigation indicated with foliar application of ascorbic acid (100 mg l-1). Using of proline in foliar way (50 mg l-1) increased significantly above parameters over the control. While, moisture, total carbohydrates and fiber of decreased significantly with adding organic manure and the highest values recorded with the untreated plant. As for the interaction effect, using ascorbic acid as antioxidant and proline ( 50 mg l-1) under chicken manure application recorded the highest values of all mentioned parameters.

Response of Three Bread Wheat to Nitrogen Fertilizer with or Without Ascorbic Acid Grown on a Clay Soil

A field experiment was conducted at Bahtim Agricultural Research Station Farm during 2012/2013 and 2013/2014 seasons. The experimental site is located at lat. 30.80, long 31.16 and 14.00 m above the mean sea level.The objective of this study was to evaluate the effect of nitrogen fertilizer rates (40, 60 and 80 kg N fed-1, with or without foliar application of ascorbic acid) on maximizing wheat yields and its attributes and grain quality of three wheat cultivars as a main-plots. The Egyptian bread wheat cultivars were Misr1, Misr2 and Gemmiza11. The experiment was designed in a split plot design with three replicates. The results indicated that: The highest significant value of spike number m-2 was noticed when wheat cultivar Mesr1 was planted compared to other bread wheat cultivars in both seasons. Also, 1000 grain weight and N uptake by wheat were significantly increased by planting Misr2. The number and weight of grain spike-1 were significantly increased when wheat cultivar Misr1 or Misr2 were planted compared to Gemmiza11 in the first season and vice versa in the 2nd one. Cultivar of Misr2 gave higher grain, straw, biological yield and harvest index as well as N, P and K uptake in grains than other cultivars in the first season only. Meanwhile, the grain, straw and biological yield as well as N, P and K uptake of wheat straw were significantly improved by planting Misr1 compared to the other cultivars in the second season. Also, data reveal that the planting of Misr1 and Gemmiza11gave the highest increase of N, P and K uptake of grain and biological yield compared to Misr2 in the second season only. Generally, in most cases, application of both nitrogen level (80 or 60 kg Nfed-1) with ascorbic acid gave the highest significant values of most yield components, grain, straw, biological yield and harvest index as well as N, P and K uptake of grain, straw, and biological as well as protein % in grain compared with other treatments in both seasons. Over all, the application of 80 or 60 kg N fed-1 with ascorbic acid to any bread wheat cultivars gave a significant increase of wheat yield components, grain, straw biological yield and harvest index as well as N, P and K uptake in grains, straw and biological and also protein % of grain in both seasons.

Impact of Irrigation Water Quality, Nitrogen Fertilization Rates and Foliar Application of Ascorbic Acid on Wheat Yield and Some Soil Properties in the North Middle Nile Delta Region

Impact of Irrigation Water Quality, Nitrogen Fertilization Rates and Foliar Application of Ascorbic Acid on Wheat Yield and Some Soil Properties in the North Middle Nile Delta Region