Foliage Treatment Research Articles

Effects of Magnetized, Chelated Iron Foliage Treatments, and Metal Halide Lamps on Plant Water Structure, Water Vapor Dynamics, and Resilience for Legumes under Water Stress

A greenhouse study was conducted to determine the effects of foliar applications of magnetized, chelated liquid iron fertilizer for increasing the drought tolerance of two legumes. The study objectives were to determine the drought tolerance effects of four treatments on foliar gas exchange, soil moisture, and plant growth for soybean (Glycine max) and velvet bean (Mucuna pruriens) plants. The plant treatments included applications with chelated liquid iron fertilizer (2.5 and 5%) with a conventional boom sprayer, with and without magnets in the spray lines, and metal halide lamps. Three gas exchange measurements were collected before applying the foliage treatments and after two water stress treatments. A foliage and metal halide lamp treatment deactivated or unlinked nine interconnected gas exchange parameters that are correlated with plant defense activities during water stress conditions. The deactivation of interconnected regulatory gas exchange functions improved metabolic efficiency, reduced stress levels, and boosted plant resilience to abiotic stressors. Also, the study findings suggest that the study treatments maintained or increased the level of biologically structured water in plant tissues and vascular systems.

Environmental fate and safety analysis of methoxyfenozide application to control litchi and longan pests

Litchi and longan pests significantly affect crop yield and quality. Chemical prevention and control are very effective for production; therefore, it is crucial to study fate assessment and appropriate field efficacy before pesticide application on crops to appropriately assess the health and ecological risks linked with these agents. This study conducted Good Agricultural Practice (GAP) field trials and laboratory experiments to elucidate the dissipation, terminal residues, and efficacy of methoxyfenozide on litchi and longan in six locations throughout China. To detect methoxyfenozide residues on litchi and longan, a QuEChERS/UPLC-MS/MS-based method was designed. The initial methoxyfenozide levels in litchi and longan ranged from 2.21–2.86 to 0.83–0.95 mg kg−1 and indicated half-lives of 5.1–5.3 and 5.3–5.7 days, respectively. After 7 days of foliage treatment, the concentrations of terminal methoxyfenozide residue were 0.78–2.61 and 0.02–1.01 mg kg−1, which were less than the established maximum residue limit for methoxyfenozide in litchi and longan. The chronic (acceptable daily intake = 0.0055–0.0331%) dietary intake risk analysis for methoxyfenozide in longan and litchi indicated acceptable concentrations of terminal residue for the general population. Methoxyfenozide in litchi and longan was readily degraded in first-order kinetics models, the degradation rate on longan was higher than that on litchi, and their dietary risks were negligible to consumers. Two hundred forty grams per liter of methoxyfenozide suspension concentrate (SC) represents a highly efficacious insecticidal dose to control litchi and longan pests and indicates a significant application potential as it is rapidly degraded and linked with reduced post-treatment residue levels.

Weed Control, Rice Safety, and Mechanism of the Novel Paddy Field Herbicide Glyamifop

Glyamifop (R&D code: FG001), (R)-(2-(4-(6-chlorobenzoxazol-2-oxy) phenoxy) propionyl) glycine ethyl ester is a newly developed aryloxyphenoxypropionate (HRAC Group 1) herbicide for weed control in paddy fields. This work determined the effect of Glyamifop on weeds and its safety for rice in the glasshouse. Glyamifop controlled the common gramineous weeds in paddy fields at 100 g a.i. ha−1: the fresh weight inhibition rates of Echinochloa crus-galli, Leptochloa chinensis, Setaria viridis, Eragrostis japonica, Digitaria sanguinalis and Panicum bisulcatum were all above 90%. It has almost no inhibitory effect on broad-leaved and cyperaceae weeds, such as Eclipta prostrata and Cyperus iria. Glyamifop inhibited cyhalofop-butyl-resistant L. chinensis, penoxsulam-resistant E. crus-galli and quinclorac-resistant E. crusgalli var. zelayensis by 100%, 99.98% and 96.37%, respectively, at 100 g a.i. ha−1, based on the fresh weight. The selectivity index of Glyamifop foliage treatment in the rice varieties japonica ‘Huaidao 5’, indica ‘Xiangliangyou 900’ and glutinous ‘Zhennuo 29’ was 5.93, 6.81 and 4.91, respectively; therefore, Glyamifop is safe for the 3 different rice varieties. Fresh weight rice inhibition rates were 7.18%, 2.99% and 7.93% at the 2.5-, 3.5- and 5.5-leaf stage, respectively, and the selectivity index was 5.18, 6.04 and 7.93, respectively, indicating that Glyamifop was safe for rice at these leaf stages. L. chinensis ACCase activity decreased with increasing Glyamifop concentration, and the inhibitory effect was similar to that of cyhalofop acid; this confirmed that Glyamifop is an ACCase inhibitor. In conclusion, Glyamifop has potential for the management of gramineous weeds as it has good activity against weeds that are resistant to common herbicides in paddy fields.

Effects of foliar application of organic acids on strawberry plants

The large economic costs and environmental impacts of iron-chelate treatments has led to the search for alternative methods and compounds to control iron (Fe) deficiency chlorosis. Strawberry plants (Fragaria x ananassa) were grown in Hoagland's nutrient solution in a greenhouse with two levels of Fe: 0 and 10 μM Fe(III)-EDDHA. After 20 days, plants growing without Fe showed typical symptoms of Fe deficiency chlorosis in young leaves. Then, the adaxial and abaxial sides of one mature or one young leaf in each plant were brushed with 10 mM malic (MA), citric (CA) or succinic (SA) acids. Eight applications were done over a two-week period. At the end of the experiment, the newly emerged (therefore untreated), young and mature leaves were sampled for nutritional and metabolomic analysis, to assess the effectiveness of treatments. Leaf regreening was monitored using a SPAD-502 apparatus, and the activity of the ferric chelate-reductase activity (FCR) was measured using root tips. Iron deficiency negatively affected biomass and leaf chlorophyll but did not increase FCR activity. Application of succinic acid alleviated the decrease in chlorophyll observed in other treatments, and the overall nutritional balance in the plant was also changed. The concentrations of two quinic acid derivatives increased under Fe deficiency and decreased in plants treated with succinic acid, and thus they are proposed as Fe stress markers. Data suggest that foliage treatments with carboxylates may be, in some cases, environmentally friendly alternatives to Fe(III)-chelates. The importance of Fe mobilization pathways in the formulation of new fertilizers is also discussed.

Tank-Mix Adjuvants Regulate the Deposition, Absorption, and Permeation Behavior of Pesticide Solutions on Rice Plant

Pesticide foliage treatment is used in agricultural production to protect plants from diseases, pests, or weeds. Tank-mix adjuvants added to the barrel can improve the effective utilization rate of pesticides. Herein, a comparative study was conducted to investigate the effect of three kinds of tank-mix adjuvant on the deposition, absorption, and permeation behavior of epoxiconazole and chlorantraniliprole solutions. Surface tension and contact angle results indicate that polyether-modified trisiloxane may be the best surface-active agent for pesticides, whereas methyl oleate and green-peel orange essential oil were found to be more suitable for improving pesticide deposition, absorption, and permeation in some cases. These findings indicate that various tank-mix adjuvants had different effects on pesticide application on plants. Appropriate tank-mix adjuvants need to be selected for comprehensive practical application.

A maceration treatment of leucaena foliage improves its nutritional value by reducing mimosine concentration

Giant leucaena produces high dry matter yields but the foliage contains mimosine, a non-protein amino acid that is toxic to animals, especially non-ruminants. Reducing mimosine concentration in foliage following harvesting may allow for greater use of Giant leucaena and mitigate the negative aspects of higher mimosine concentration in some varieties. We evaluated two methods for post-harvest treatment of foliage of a highly productive interspecific hybrid variety ‘KX2’ for reducing mimosine concentration: (i) maceration treatment; and (ii) extraction with 0.1 N HCl. Mimosine as a percentage of leaf dry matter ranged from less than 1% DM to around 3% DM. Although both methods reduced mimosine concentration, extraction by 0.1 N HCl also reduced gross energy, protein and carbohydrate concentrations of leucaena foliage. The maceration treatment, on the other hand, caused little reduction in crude protein and crude fat concentrations but markedly increased the carbohydrate concentration. ADF and NDF concentrations were also reduced as a result of maceration treatment. The estimated gross energy concentration in macerated foliage was not significantly lower than in unprocessed foliage. A suitable mechanical method for post-harvest maceration of leucaena foliage, e.g. a wood-chipping machine, could be used to reduce mimosine concentration in the foliage, making it safer for feeding to livestock and enhancing the feed value, especially for non-ruminants. These methods should be tested by conducting feeding studies to determine the possible benefits in animal performance from feeding macerated foliage.

Effects of Magnetized, Chelated Iron Foliage Treatments on Foliar Physiology, Plant Growth and Drought Tolerance for Two Legume Species

A greenhouse study was conducted to determine the effects of foliar applications of magnetized, chelated liquid iron fertilizer for increasing the drought tolerance of two legumes. Study objectives were to determine the drought tolerance effects of four treatments on foliar gas exchange, soil moisture, and plant growth for velvet bean (Mucuns pruriens) and soybean (Gylcine max) plants. The four foliage treatments included applications with chelated liquid iron fertilizer (2.5 and 5%) with a conventional boom sprayer, with and without magnets in the spray lines. Physiological measurements were collected before foliar treatments and again after a 24-day deficit irrigation schedule. Physicochemical water properties were measured for each of the foliar treatments. Photosynthesis rates were 5.98, 2.04 and 3.19 µmol/m2/s for the control, non-magnetized and magnetized fertilizer treatments (2.5%), respectively, after completing the deficit irrigation schedule. Instantaneous water use efficiency (IWUE) was 0.60, 0.28 and 1.02 for the control, non-magnetized and magnetized fertilizer treatments (2.5%), respectively, after completing the deficit irrigation schedule. Photosynthesis and IWUE increased 56 and 263% for the magnetized fertilizer treatment (2.5%) compared to the non-magnetized foliar treatment, when averaged across both legume species. Photosynthesis and IWUE increased as electrical conductivity increased and oxidation reduction potential (ORP) decreased in absolute terms. A single foliar application resulted in aberrant physiological responses that are contrary to very widely held plant defense theories involving abiotic stressors. The single application improved the photosynthesis and water use efficiency for water stressed legumes emphasizing the need to better understand the relationships between water quality, plant bioenergetics, and stress physiology. Improved drought tolerance in row crops such as dry beans and soybeans, with a single magnetized fertilizer application, would be cost effective and easily adapted into current cropping systems. Interactions among physicochemical water properties, bioenergetics, plant metabolism, and crop stress physiology need to be further investigated in order to improve the quality of irrigation water to enhance drought tolerance of field crops.

Enantioselective fate of dinotefuran from tomato cultivation to home canning for refining dietary exposure

Understanding the enantioselective fate of chiral neonicotinoid dinotefuran is of vital importance for accurate dietary exposure assessment and food safety regulation. The study investigated the enantioselectivity in respect to dissipation, metabolism, and removal, of dinotefuran from tomato cultivation to tomato paste processing. The chiral analytical method of dinotefuran, UF and DN was developed in tomato using ultrahigh performance supercritical fluid chromatography/tandem mass spectrometry. Under greenhouse cultivation R-dinotefuran preferentially degraded (T1/2, 9.1−12.6 days), resulting in relative enrichment of S-dinotefuran (T1/2, 10.3−13.3 days) by foliage and root uptake pathways. (−)-UF generated at a faster rate and was more persistent than its antipode in tomato by foliage treatment. Furthermore, changes in the enantiomeric removal and enantioselectivity orientation of dinotefuran and metabolites were evaluated during home canning of tomato paste, including washing, peeling, homogenization, simmering, and sterilization. Peeling played the key role in reducing S-dinotefuran by 67.3% and R-dinotefuran by 69.9% with processing factor of 0.313 and 0.287, respectively. Simmering was the most effective way to remove UF enantiomers (Pf, 0.336–0.421) by elevated temperature. This study sheds light on the chiral profiles of the fate of dinotefuran from cultivation to processing, providing scientific importance to protect human health from hazardous effects.

Induction of 2-cyanoethyl-isoxazolin-5-one as an antifeedant against the tobacco cutworm (Spodoptera litura) by jasmonic acid in sweet pea leaf.

Although sweet pea (Lathyrus odoratus) beans contain toxic β-aminopropionitrile, the plant itself is readily attacked by insects and is, therefore, protected through the use of pesticides. Consequently, the induction of L. odoratus resistance to insect attack via exogenous treatment is promising for pest control development. Screening of inducible elicitor effects showed that treatment of sweet pea foliage with jasmonic acid (JA) can induce antifeeding-based resistance to tobacco cutworm (Spodoptera litura) larvae. Spectroscopic analysis identified 2-cyanoethyl-isoxazolin-5-one (2-CEIX) as the antifeedant with a half-maximal effective concentration of 33.6µmol/g fr. wt., i.e., exogenous JA treatment induced antifeeding activity due to the accumulation of 2-CEIX. Moreover, 2-CEIX-induced mortality of S. litura larvae was evaluated by a dipping test and the half-maximal lethal dose was determined to be 5.9 mg/mL. Therefore, 2-CEIX was concluded to be a suitable induced resistance target for elicitors or a lead compound for insecticide development.

Dissipation and translocation of saisenxin in tobacco and soil under conventional field and controlled laboratory conditions

The aim of this investigation was to investigate the fate and translocation characteristics of saisenxin (SSX), a novel organic zinc fungicide, in the environment and tobacco plants under conventional field and laboratory conditions. A rapid and sensitive analytical technique based on high-performance liquid chromatography was used for determination of SSX, in soil samples and tobacco leaf, stem and root samples. The method had satisfactiry linearity (R2 = 0.9999) and the limits of detection and of quantitation of the target compound were 0.06 and 0.20 mg kg−1, respectively. The average recoveries were in the range of 89.74–94.24% in soil, leaf, stem and root samples, with relative standard deviations of <8%. For conventional field trials, the half-life (t1/2) of SSX was 5.9–6.5 days in soil and 4.8–5.3 days in tobacco leaves; the corresponding values under controlled laboratory conditions were extended to 7.1 and 7.6 days. The translocation factor (TF) values were in the range of 0–2.25 and 0–0.25 for foliage and root irrigation treatments, respectively. The TFs of SSX in tobacco indicated that tobacco had a high ability to transfer SSX upward.

Cell Wall Reinforcement in the Potato Tuber Periderm After Crop Treatment with Potassium Phosphite

The potato periderm is the outer tissue of potato tubers that protects the tuber from pathogen attack, dehydration, and wounding during harvest and storage. It is of secondary origin and replaces the epidermis early during tuber development. The outer layers of the periderm consist of cells with suberized walls. Phosphite compounds (Phi) are inorganic salts from phosphorous acid, and in previous works, we demonstrated that they are effective in protecting potato plants from biotic stress caused by different pathogens and also against UV-B radiation. The aim of the present work was to study the effect of Phi on the post-harvest potato periderm structure, cell wall components, and related enzymes. In three years of field experiments, potassium phosphite (KPhi) applications were compared with an untreated control. KPhi was applied to seed tubers before planting combined with foliage application of a conventional fungicide, to seed tubers and foliage without conventional fungicide, or to seed tubers combined with foliage application of KPhi plus a conventional fungicide. Observations of periderm sections showed increases in suberin and pectin depositions in post-harvest tubers from KPhi-treated plants compared to tubers from non-treated plants. In addition, peroxidase activity increased in the periderm tissue. The activity of laccases and superoxide dismutase was measured in the protein extract of the periderm, and an increase due to KPhi treatment was detected. The results presented here suggest that early KPhi treatment to seed tubers followed by foliage treatment leads to reinforcement of the cell wall of periderm cells, which represents a benefit for future response to stresses.

Inducing Systemic Resistance against Cucumber Mosaic Cucumovirus using Streptomyces spp.

Seven Streptomyces spp. were screened for their potential to protect cucumber plants against Cucumber mosaic virus (CMV). Foliage treatment with the Streptomyces culture filtrates resulted in high reduction of the level of disease severity of CMV infection. In contrast, soil treatments were less effective in virus inhibition. Concerning foliage treatment, culture filtrates of S. griseorebens and S. cavourensis showed the highest inhibitory effect (90% and 85%, respectively) when applied 48 hrs prior to virus inoculation. On the other hand, soaking of cucumber seeds for 2hrs in the Streptomyces culture filtrates resulted in the highest viral inhibition. Generally, S. griseorebens recorded the highest percentage of viral inhibition (65%). Direct ELISA was carried-out as a diagnostic tool at the beginning and throughout this study. A number of de-novo synthesized proteins (induced proteins) were detected by polyacrylamide-gel electrophoretic analysis in cucumber leaves treated with culture filtrates of Streptomyces spp. relative to the control treatment. Considerable increase in total phenols as well as peroxidase and polyphenol oxidase activity levels in treated plants before virus inoculation were recorded comparing with the healthy and infected control plants. The highest values were observed in treated cucumber plants with S. griseorebens collected 14 days post virus inoculation.

Dissipation and distribution of chlorpyrifos in selected vegetables through foliage and root uptake.

Dissipation, distribution and uptake pathways of chlorpyrifos were investigated in pakchoi (Brassica chinensis L.) and lettuce (Lactuca sativa) with foliage treatments under a greenhouse trial and root treatments under a hydroponic experiment. The dissipation trends were similar for chlorpyrifos in pakchoi and lettuce with different treatments. More than 94% of chlorpyrifos was degraded in the samples for both of the vegetables 21 days after the foliage treatments. For the root treatment, the dissipation rate of chlorpyrifos in pakchoi and lettuce at the low concentration was greater than 93%, however, for the high concentrations, the dissipation rates were all under 90%. Both shoots and roots of the vegetables were able to absorb chlorpyrifos from the environment and distribute it inside the plants. Root concentration factor (RCF) values at different concentrations with the hydroponic experiment ranged from 5 to 39 for pakchoi, and from 14 to 35 for lettuce. The translocation factor (TF) representing the capability of the vegetables to translocate contaminants was significantly different for pakchoi and lettuce with foliage and root treatments. The values of TF with foliage treatments ranged from 0.003 to 0.22 for pakchoi, and from 0.032 to 1.63 for lettuce. The values of TF with root treatments ranged from 0.01 to 0.17 for pakchoi, and from 0.003 to 0.23 for lettuce. Significant difference of TF was found between pakchoi and lettuce with foliage treatments, and at high concentrations (10 and 50 mg L(-1)) with root treatments as well. However, there was no significant difference of TF between pakchoi and lettuce at 1 mg L(-1) with root treatment.

맥류재배에서 제초제에 따른 잡초방제 효과

This study was conducted to evaluate the efficacy of herbicide in winter cereal crops, barley and wheat. The efficacy of soil-applied herbicides, linuron EC and pendimethalin, thiobencarb GR was greater than that of butachlor EC. Linuron EC controlled average 95% and pendimethalin, thiobencarb GR controlled average 97% within 150 days after soil-applied treatment. When compared with untreated control, no visual injuries were detected at single and double dosage of linuron EC and pendimethalin, thiobencarb GR. The yield of barley increased in linuron EC and wheat increased in pendimethalin, thiobencarb GR treatments when compared with butachlor EC. The efficacy of foliage treatment, bentazone+propanil ME was greater than that of bentazone SL. Bentazone+propanil ME controlled average 96% in barley and 97% in wheat, within 30 days after foliage treatment. When compared with untreated control, no visual injuries were detected at single and double dosage of bentazone+propanil ME. The yield of barley and wheat increased in bentazone+propanil ME treatments when compared with bentazone SL. Based on these data, combinations of these herbicides could be applied to provide effective weed management in barley and wheat field.

Effectiveness of 14 Fungicides for Suppressing Lesions Caused by Phytophthora capsici on Inoculated Stems of Chile Pepper Seedlings

Phytophthora blight, caused by the oomycete pathogen Phytophthora capsici, is an economically important disease in bell and chile pepper. Fourteen different fungicides were evaluated with respect to inhibition of stem lesion growth on chile pepper seedlings inoculated with mycelium or with zoospores of P. capsici 1 or 3 weeks after treatment of plant foliage and stems or roots. Fungicides containing ametoctradin + dimethomorph and fluopicolide were the most effective among tested products in both experiments across eight trial parameters (inoculum type, inoculation time after treatment, and fungicide application site). Other active ingredients, including acibenzolar-S-methyl, dimethomorph, fenamidone, ethaboxam, mandipropamid, mefenoxam, and oxathiapiprolin, were most effective in reducing stem lesion growth in three to seven of the eight trial parameters evaluated. Compared to nontreated plants, stem lesion inhibition ranged from 84.1 to 100%. Data from these trials demonstrate the comparative effectiveness of tested products under controlled environmental conditions favorable for disease development; however, confirmation of these findings is required in field trials, where plant and environmental conditions will be variable. Accepted for publication 18 September 2014. Published 1 November 2014.

Effects of Timing of Paddy Field Drainage for Foliage Treatment Herbicide Spraying and Nitrogen Top-dressing on the Growth and Yield of Dry Direct Seeded Rice in Hokkaido, Japan

Effects of Timing of Paddy Field Drainage for Foliage Treatment Herbicide Spraying and Nitrogen Top-dressing on the Growth and Yield of Dry Direct Seeded Rice in Hokkaido, Japan

Chiral fungicide triadimefon and triadimenol: Stereoselective transformation in greenhouse crops and soil, and toxicity to Daphnia magna

Various chiral pesticides are used in greenhouses to ensure high crop yields. However, detailed knowledge on the environmental behavior of such chiral contaminants with respect to enantioselectivity in the greenhouse has received little attention so far. Here, the widely used fungicide triadimefon was chosen as a “chiral probe” to investigate its enantioselective degradation and formation of triadimenol in greenhouse tomato, cucumber, and soil under different application modes. In addition, the stereoselectivity of individual isomers of triadimefon and triadimenol in aquatic toxicity were first studied. Significant differences in their acute toxicity to Daphnia magna were observed among the isomers. Under foliage application or soil irrigation application, S-(+)-triadimefon was preferentially degraded, resulting in relative enrichment of the more toxic R-(−)-enantiomer in tomato, cucumber, and soil. Further enantioselective analysis of converted triadimenol showed that the compositions of the four product stereoisomers were different and closely dependent on environmental conditions: the most toxic RS-(+)-triadimenol was the most preferentially produced isomer in tomato under foliage treatment, while the RR-(+)-triadimenol was proved to be the highest amount of metabolite isomer in cucumber and soil under both treatment modes and in tomato under soil treatment.

Skuteczność zwalczania chwastów w kukurydzy z użyciem dwóch zabiegów nalistnych

In the years 2011–2012, three field experiments were carried out in maize to evaluate effect of weed control with herbicides applied at reduced doses as two foliage treatments. Herbicides were applied in the following system: early foliage treatment at the 2–3 leaf stage of maize with Lumax 537,5 SE (s-metolachlor + terbuthylazine + mesotrione) at 1.5 l/ha and second treatment at the 5–7 leaf stage of maize with Hector 53,6 WG (nicosulfuron + rimsulfuron) at the 35 g/ha, Milagro 040 SC (nicosulfuron) at 0.5 l/ha, Titus 25 WG (rimsulfuron) at 30 g/ha or Maister 310 WG (foramsulfuron + iodosulfuron). Adjuvant Atpolan Bio 80 SL at the dose of 1.0 l/ha was added to herbicides in the second foliage treatment. The conducted experiments showed the advantages of the application of two foliage treatments with the use of herbicides at reduced doses against Echinochloa crus-galli and broad-leaved weeds in maize. There were no symptoms of the phytotoxic effect of the applied herbicides found on maize plants in performed experiments. High effectiveness of weed control in maize with the use of different systems of herbicide application affected favorably maize yield and weight of 1000 grains. No effect on protein, oil and starch content was observed. W latach 2011–2012 przeprowadzono trzy ścisłe doświadczenia ze stosowaniem herbicydów w dawkach obniżonych, w systemie dwóch zabiegów nalistnych w kukurydzy. Do pierwszego zabiegu w fazie 2–3 liści kukurydzy stosowano Lumax 537,5 SE (mezotrion + S-metolachlor + terbutyloazyna) w dawce 1,5 l/ha. Do następnych zabiegów w fazie 5–7 liści stosowano herbicydy: Hector 53,6 WG (nikosulfuron + rimsulfuron) w dawce 35 g/ha, Milagro 040 SC (nikosulfuron) w dawce 0,5 l/ha, Titus 25 WG (rimsulfuron) w dawce 30 g/ha lub Maister 310 WG (foramsulfuron + jodosulfuron) w dawce 60 g/ha. Poszczególne dawki herbicydów były obniżone od podstawowych w granicach 40–50%. W drugim zabiegu do wszystkich herbicydów dodano adiuwant Atpolan Bio 80 EC w dawce 1 l/ha. Zastosowane kombinacje wykazały bardzo dobry efekt w zwalczaniu chwastnicy jednostronnej oraz gatunków dwuliściennych. Nie wykazały fitotoksycznego działania w stosunku do kukurydzy. Stwierdzono istotny wpływ zastosowanego systemu odchwaszczania na przyrost plonu ziarna kukurydzy i wzrost masy 1000 ziaren. Nie stwierdzono wpływu zabiegów herbicydowych na procentową zawartość białka, tłuszczu i skrobi.

잔디밭잡초 발생현황과 방제기술

잔디밭에 발생하는 잡초를 파악하고 효율적인 방제체계를 확립하기 위하여 문헌조사를 하였다. 공원 등 잔디밭에 발생하는 잡초는 방동사니대가리 등 사초과 3종, 바랭이, 새포아풀 등 화본과 9종, 쑥, 꽃다지, 피막이 등 광엽잡초 25종 등 총 16과 37종이나, 산지와 인접한 묘지 잔디밭에는 53과 196종의 잡초가 발생하고 있다. 그러므로 산지와 인접한 골프장에서는 묘지에서 발생되는 잡초들이 만연할 가능성이 많다. 잔디밭에 발생되는 잡초를 효율적으로 관리하기 위해 등록된 제초제는 2012년 5월말 현재 토양처리제는 디클로베닐입제, 메티오졸린유제, 옥사지클로메폰액상수화제, 이마자퀸입제 등 32품목, 경엽처리제로는 메타미포프유제, 비페녹스.플루세토설퓨론입상수화제, 플라자설퓨론수화제, 트리플록시설푸론소듐입상수화제 등 35종 총 67품목이 있다. 잔디밭에서의 잡초방제체계는 일반적으로 토양처리제는 3~4월과 8~9월에 2회 처리하고, 경엽처리제는 6~7월에 처리하면 효과적으로 잡초를 방제할 수 있다. Literatures were reviewed to understand the weeds occurring on the lawn and the effective control methods of them. Thirty-seven species of 16 families including three species of Cyperaceae for example Cyperus sanguinolentus, nine species of Poaceae including Digitalis ciliaris, Poa repens, etc., and 25 species of broad-leaved weeds such as Artemisia princeps, Draba nemorosa, Hydrocotyle sibthorpioides were occurred on the lawns. One hundred ninety-six weed species of 53 families were occurred on a tomb graveyard adjacent to the mountain. Therefore, it is possible to be infested by the weeds occurring on the graveyard on the golf course adjacent to the mountains. There are 67 items, 32 kinds of the soil treatment herbicides including dichlobenil GR, methiozolin EC, oxaziclomefone SC, imaxaquin GR et al, and 35 kinds of the foliar treatment herbicides including metamifop EC, bifenox flucetosulfuron WG, flazasulfuron WP, trifloxysulfuron-sodium WG et al. registered at the end of May 2012 for efficient management of the weeds occurring on the lawn. For effective management of the weeds, the herbicide can be generally applied twice a year on March to April and August to September for soil treatment and once a year on June to July for the foliage treatment.

Colonization of geranium foliage by Clonostachys rosea f. catenulata, a biological control agent of botrytis grey mould

The ecological requirements for the colonization of geranium leaves by the biocontrol agent Clonostachys rosea f. catenulata strain J1446 were investigated. Although this biocontrol agent is a soil-inhabiting fungus, treatment of geranium foliage with the agent can reduce grey mould caused by Botrytis cinerea in the greenhouse. To characterize the extent of foliar colonization, a GUS-transformed isolate of C. rosea f. catenulata was applied to foliage of two geranium cultivars, Pelargonium × hortorum and Pelargonium × domesticum . Population levels of C. rosea f. catenulata were found to be highest on senescent leaves and stems, followed by fully expanded leaves, and lowest on newly emerged leaves of both cultivars. Optimum temperature for leaf and petiole colonization was 20–25 °C for both cultivars. The biocontrol agent required at least 12 h of continuous leaf wetness to achieve maximum population densities on the leaves and stems of both cultivars. On whole plants, colonization was significantly higher on wounded leaves, stems, and senescing leaves compared with that on nonwounded leaves, stems, and mature leaves, respectively. GUS staining indicated that the fungus preferentially colonized the wound sites of leaves and the cut portions of stems. Results indicate that this biocontrol agent can successfully colonize the foliage of geraniums, thus demonstrating the endophytic ability of C. rosea f. catenulata in both root and foliar tissues.