Fold Increase In Activity Research Articles

ブドウ球菌コアグラーゼの精製と性状に関する研究

A purified coagulase was obtained from a culture filtrate of Staphylococcus aureus St-213 which proved to be homogenous in immunological, electrophoretical and Spinco Model E ultracentrifugal studies. Treatment of the material with trichloroacetic acid prior to passing through Dowex-1 and Diethylaminoethyl-Sephadex A-50 columns yielded a product which showed a 280 fold increase in activity per mg of nitrogen than the crude culture filtrate. The sedimentation coefficient of the treated material was S20, w=1.59S.The treated material was composed of 9 amino acids (i.e. lysin, aspartate, threonine, serine, glutamine, glycine, alanine, valine and methionine) and lacked carbohydrates, lipids and phosphates. Therefore, this purified material may be classified as a purified protein derivative (PPD) capable of exhibiting a specific coagulase type reaction against homologous and heterologous type antiserum when tested immunologically using the agar gel diffusion technique.

BIOLOGICAL STUDIES OF THE GONADOTROPIC PRINCIPLES IN SHEEP PITUITARY SUBSTANCE1

IN A RECENT publication we described the preparation of two gonadotropic principles from sheep pituitary, namely a follicle stimulating hormone (FSH) and an interstitial cell stimulating hormone (ICSH) (i). A ioo′fold increase in activity over th potency of the dried glands was achieved for each fraction and either was found to befree of the other component up to 10 to 20 times the M.E.D. We also briefly stated that our biological studies of these two gonadotropic fractions indicated that such physiological re′sponses as synergism (activation), luteinisation and antagonism can be dem′onstrated by injecting a combination of these two factors in certain pro′portio and by various routes. In the present communication we wish to report the results of these combination experiments. Definition of Units and Standardization of FSH andICSH In order to be able to compare the biological results quantitatively we adopte the following physiological