Flux Measurements Research Articles

Biocalorimetry-aided monitoring of fungal pretreatment of lignocellulosic agricultural residues

The present study aimed to investigate whether and how non-invasive biocalorimetric measurements could serve for process monitoring of fungal pretreatment during solid-state fermentation (SSF) of lignocellulosic agricultural residues such as wheat straw. Seven filamentous fungi representing different lignocellulose decay types were employed. Water-soluble sugars being immediately available after fungal pretreatment and those becoming water-extractable after enzymatic digestion of pretreated wheat straw with hydrolysing (hemi)cellulases were considered to constitute the total bioaccessible sugar fraction. The latter was used to indicate the success of pretreatments and linked to corresponding species-specific metabolic heat yield coefficients (YQ/X) derived from metabolic heat flux measurements during fungal wheat straw colonisation. An YQ/X range of about 120 to 140 kJ/g was seemingly optimal for pretreatment upon consideration of all investigated fungi and application of a non-linear Gaussian fitting model. Upon exclusion from analysis of the brown-rot basidiomycete Gloeophyllum trabeum, which differs from all other here investigated fungi in employing extracellular Fenton chemistry for lignocellulose decomposition, a linear relationship where amounts of total bioaccessible sugars were suggested to increase with increasing YQ/X values was obtained. It remains to be elucidated whether an YQ/X range being optimal for fungal pretreatment could firmly be established, or if the sugar accessibility for post-treatment generally increases with increasing YQ/X values as long as “conventional” enzymatic, i.e. (hemi)cellulase-based, lignocellulose decomposition mechanisms are operative. In any case, metabolic heat measurement–derived parameters such as YQ/X values may become very valuable tools supporting the assessment of the suitability of different fungal species for pretreatment of lignocellulosic substrates.Key points• Biocalorimetry was used to monitor wheat straw pretreatment with seven filamentous fungi.• Metabolic heat yield coefficients (YQ/X) seem to indicate pretreatment success.• YQ/X values may support the selection of suitable fungal strains for pretreatment.

Development and experimental testing of an innovative nondestructive thermal sensor utilizing the thermal interrogation method for detecting leakage in water plastic pipes

Leak detection in water pipelines is still an important issue in real-world industrial systems. Thus, a new nondestructive thermal sensor design based on the thermal interrogation method has been developed to detect and estimate the real-time leaks in water plastic pipes without environmental interference. The proposed thermal interrogation method utilizes a combination of heat flux and temperature measurements on the upstream and downstream outside plastic pipe surfaces. These measurements are obtained by using heat flux sensors and thermocouples that are covered by thin-film polyimide electric heaters. The interrogation method approach delivers that at constant input heat flux, the difference in sensor temperature values is dependent on the leakage volume flow rates. The sensor was tested over a range of water leakage volume flow rate from 0.1 m3/h to 1.3 m3/h and a range of water temperature from 21 °C to 25 °C. The water leakage volume flow rates were correlated to the difference in temperature values between the upstream and downstream sensors. The measurement results reveal that this sensor can be used to detect and estimate the real-time water leakage volume flow rate with high accuracy and repeatability for plastic pipes.

Mapping urban haze.

Direct airborne measurements of emission fluxes reveal major sources over California.

Mechanisms behind high N2O emissions from livestock enclosures in Kenya revealed by dual-isotope and functional gene analyses

Livestock manure contributes to global warming due to greenhouse gas (GHG) emissions, especially nitrous oxide (N2O) and methane (CH4). In the arid and semi-arid lands of Sub-Saharan Africa (SSA), extensive pastoral grazing systems are common, with cattle grazing in the savanna during the day and kept in enclosures (called bomas in Kenya) during the night. Manure is usually not removed from bomas but left to accumulate, leading to excessive local nitrogen loads, making these bomas an overlooked N2O emission hotspot in SSA that is currently not accounted for in national and regional GHG budgets. Here, we present the first in-situ isotope measurements of N2O fluxes from 37 cattle bomas along an age gradient ranging from 0 to 5 years after boma abandonment in Kenya along with functional gene analysis of soil and manure samples. The isotopic composition of the emitted N2O from bomas suggests that on average 91 ± 8% N2O was produced via bacterial denitrification and/or nitrifier denitrification, with little variation across boma age class. We also found high levels of N2O reduction to N2 across all sample sites (81 ± 9%), indicating high levels of N2O consumption. The abundances of denitrification-related genes (nirK and narG) were significantly higher than those of nitrification-related genes (amoA: AOA and AOB) in the cattle manure samples taken from the bomas, corroborating N2O emissions largely being attributed to denitrification. Significant abundance of the reduction-related gene (nosZ) also corroborated the high potential for microbial N2O reduction in bomas. Thus, by combining dual-isotope and functional gene analysis, we were able to identify source processes that govern N2O emissions from these systems. More generally, making use of the manure by spreading it in the vicinity of the bomas or on dedicated forage plots could provide a win-win by enhancing savanna productivity while simultaneously mitigating GHG emissions.

Temperature-dependent emissions dominate aerosol and ozone formation in Los Angeles.

Despite declines in transportation emissions, urban North America and Europe still face unhealthy air pollution levels. This has challenged conventional understanding of the sources of their volatile organic compound (VOC) precursors. Using airborne flux measurements to map emissions of a wide range of VOCs, we demonstrate that biogenic terpenoid emissions contribute ~60% of emitted VOC OH reactivity, ozone, and secondary organic aerosol formation potential in summertime Los Angeles and that this contribution strongly increases with temperature. This implies that control of nitrogen oxides is key to reducing ozone formation in Los Angeles. We also show some anthropogenic VOC emissions increase with temperature, which is an effect not represented in current inventories. Air pollution mitigation efforts must consider that climate warming will strongly change emission amounts and composition.

A Reference Meteor Magnitude for Intercomparable Fluxes

The rate at which meteors pass through Earth’s atmosphere has been measured or estimated many times over; existing flux measurements span at least 12 astronomical magnitudes, or roughly five decades in mass. Unfortunately, the common practice of scaling flux to a universal reference magnitude of +6.5 tends to collapse the magnitude or mass dimension. Furthermore, results from different observation networks can appear discrepant due solely to the use of different assumed population indices, and readers cannot resolve this discrepancy without access to magnitude data. We present an alternate choice of reference magnitude that is representative of the observed meteors and minimizes the dependence of flux on population index. We apply this choice to measurements of recent Orionid meteor shower fluxes to illustrate its usefulness for synthesizing independent flux measurements.

Quantifying the carbon sequestration potential of different soil management practices aimed at increasing organic content in soil and reducing the usage of chemical inputs

<p>The global imperative to address climate change and promote sustainable agriculture has underscored the critical role of soil management practices in enhancing carbon sequestration and reducing greenhouse gas emissions. This research focuses on quantifying the carbon sequestration potential of diverse soil management techniques aimed at increasing organic content in soil while minimizing the use of chemical inputs. It integrates a multidisciplinary approach, conducting field experiments across diverse agroecological regions with different soil types and cropping systems. Key practices under investigation include cover cropping, crop rotations, conservation tillage, compost application, organic amendments, integrated pest management (IPM), and precision agriculture. The study assesses their impact on soil organic carbon levels, greenhouse gas emissions, soil health indicators, and crop productivity through comprehensive data collection and analysis techniques. Statistical analyses and modeling are employed to quantify carbon sequestration rates, evaluate treatment effects, and assess environmental and agronomic benefits. These practices are implemented in controlled trials to assess their impact on soil organic carbon (SOC) levels, greenhouse gas emissions (CO2, CH4, N2O), soil health indicators (microbial biomass, nutrient cycling, soil structure), and crop productivity. Data collection and analysis techniques involve comprehensive soil sampling, greenhouse gas flux measurements, soil health assessments, and crop yield monitoring over multiple growing seasons. Statistical analyses and modeling approaches are employed to quantify the carbon sequestration rates, assess treatment effects on soil health and greenhouse gas emissions, and evaluate the overall environmental and agronomic benefits of the soil management practices. The study aims to contribute valuable insights into evidence-based strategies for promoting sustainable soil management practices that enhance carbon sequestration while maintaining or improving agricultural productivity. The findings will have implications for informing agricultural policies, guiding farm management decisions, and advancing research on climate-smart agriculture. By quantifying the carbon sequestration potential of different soil management practices, this research contributes to the broader goal of achieving climate resilience, environmental sustainability, and food security in agricultural systems.</p>

Modeling of phosphate flux induced by flood resuspension on a macrotidal estuarine mudflat (Seine, France)

Coastal marine sediments can be either major scrubbers or eutrophication contributors to surface waters. Standard methods for direct measurement of nutrient fluxes at the sediment-water interface do not consider hydrodynamic forcing although several ex-situ studies suggest that sediment resuspension can dramatically increase dissolved fluxes. We provide a new model to quantify dissolved phosphate (PO43−) resuspension flux (JR) based on physical representation of its identified components: diffusion stimulation by exposure of deeper sediment layer with higher PO43− concentration in the porewater (JD), pore water mixing with overlying water (JM) and net adsorption/desorption from suspended sediments (JK). This approach was applied to field data from a Seine intertidal mudflat periodically submitted to millimetric erosion. On a tidal scale, the model output reveals a JR of 272.3 ± 360.0 μmol m−2 h−1 (± 52% from parameter uncertainty), well above flux calculated by application of Fick's first law (0.15 ± 0.85 μmol m−2 h−1) or by ex situ core incubation (40.8 μmol m−2 h−1). Iron bound phosphorus within suboxic layers buffers PO43− concentrations in superficial sediments leading to negligible contributions of JD and JM to total fluxes. Conversely, JK appears to be the main exchange pathway, even though improvements in turbidity measurement would allow this term to be defined more precisely. Correction required to enhance and control model robustness are described. These results show the importance of considering the dissolved PO43− resuspension flux in dynamic environments.

Induction of DEPP1 by HIF Mediates Multiple Hallmarks of Ischemic Cardiomyopathy.

HIF (hypoxia inducible factor) regulates many aspects of cardiac function. We and others previously showed that chronic HIF activation in the heart in mouse models phenocopies multiple features of ischemic cardiomyopathy in humans, including mitochondrial loss, lipid accumulation, and systolic cardiac dysfunction. In some settings, HIF also causes the loss of peroxisomes. How, mechanistically, HIF promotes cardiac dysfunction is an open question. We used mice lacking cardiac pVHL (von Hippel-Lindau protein) to investigate how chronic HIF activation causes multiple features of ischemic cardiomyopathy, such as autophagy induction and lipid accumulation. We performed immunoblot assays, RNA sequencing, mitochondrial and peroxisomal autophagy flux measurements, and live cell imaging on hearts and isolated cardiomyocytes. We used CRISPR-Cas9 gene editing in mice to validate a novel mediator of cardiac dysfunction in the setting of chronic HIF activation. We identify a previously unknown pathway by which cardiac HIF activation promotes the loss of mitochondria and peroxisomes. We found that DEPP1 (decidual protein induced by progesterone 1) is induced under hypoxia in a HIF-dependent manner and localizes inside mitochondria. DEPP1 is both necessary and sufficient for hypoxia-induced autophagy and triglyceride accumulation in cardiomyocytes ex vivo. DEPP1 loss increases cardiomyocyte survival in the setting of chronic HIF activation ex vivo, and whole-body Depp1 loss decreases cardiac dysfunction in hearts with chronic HIF activation caused by VHL loss in vivo. Our findings identify DEPP1 as a key component in the cardiac remodeling that occurs with chronic ischemia.

Comparison between Direct and Indirect Heat Flux Measurement Techniques: Preliminary Laboratory Tests

Direct and indirect approaches can be employed for estimating the heat flow through components in different application fields. In the building sector, the thermometric method is often applied by professionals for thermal transmittance evaluations. However, miscalculations can derive from inaccurate total heat transfer coefficients, and a consensus regarding the appropriate value to employ remains to be determined. Here, an apparatus was realized for laboratory tests and heat flux measurements were performed following direct and indirect approaches. Data acquired through a common heat flow sensor were compared with those computed through a post-processing based on radiative and convective estimations. The results were affected by the specific correlation adopted for computing the convective coefficients, with the percentage differences ranging from −9.8% to −0.4%. New measurement systems could be designed for automatically computing heat fluxes through indirect approaches, thus providing alternative solutions in the panorama of non-destructive tests for building energy diagnosis.

Measurement of solar pp neutrino flux using electron recoil data from PandaX-4T commissioning run* *This project is supported in part by the grants from the National Science Foundation of China (12090060, 12090063, 12105052, 12005131, 11905128, 11925502), the Office of Science and Technology, Shanghai Municipal Government (22JC1410100), and the National Science Foundation of Sichuan Province, China (2024NSFSC1371). We are grateful for

The proton-proton ( ) fusion chain dominates the neutrino production in the Sun. The uncertainty of the predicted neutrino flux is at the sub-percent level, whereas that of the best measurement is (10%). In this study, for the first time, we measure solar neutrinos in the electron recoil energy range from 24 to 144 keV using the PandaX-4T commissioning data with 0.63 tonne year exposure. The neutrino flux is determined as , which is consistent with the Standard Solar Model and existing measurements, corresponding to an upper flux limit of at 90% C.L..

Numerical investigation of mixing and heat transfer in a 7.9 m JP-8 pool fire

The response of objects engulfed in, and adjacent to, large-scale pool fires is of interest in accident and safety assessments. In this study, Fuego, a low-Mach turbulent reacting flow code was used to study conjugate heat transfer in a 7.9 m diameter JP-8 pool fire. Simulations were designed to replicate past experimental measurements (Blanchat et al., 2006) of incident heat flux to three cylindrical calorimeters in and around the pool fire. Two turbulent combustion models were compared directly - the eddy dissipation concept and a more recently developed unsteady flamelet model. First and second order spatial and temporal discretization schemes were also compared to assess the performance of low-dissipation numerical operators. Heat flux predictions to the transportation size calorimeter outside the fire were within experimental uncertainties. Inside the fire, experimental measurements were higher than predicted values and may have been a consequence of soot deposition and augmented participating media radiation from soot and fuel vapor. Simulation predictions improved in cases where turbulent kinetic energy and mixing were more resolved. This work, and others referenced herein, suggest that spatial resolution on the order of 0.5–1.0 cm may be required to fully resolve fluid instabilities, vortex production between fire plumes and crosswind, soot production, and fuel-air mixing. This presents a substantial computational challenge for safety assessments of engulfed objects in fully turbulent pool fires.

Search for Periodic Time Variations of the Solar B8 Neutrino Flux between 1996 and 2018 in Super-Kamiokande

We report a search for time variations of the solar B8 neutrino flux using 5804 live days of Super-Kamiokande data collected between May 31, 1996, and May 30, 2018. Super-Kamiokande measured the precise time of each solar neutrino interaction over 22 calendar years to search for solar neutrino flux modulations with unprecedented precision. Periodic modulations are searched for in a dataset comprising five-day interval solar neutrino flux measurements with a maximum likelihood method. We also applied the Lomb-Scargle method to this dataset to compare it with previous reports. The only significant modulation found is due to the elliptic orbit of the Earth around the Sun. The observed modulation is consistent with astronomical data: we measured an eccentricity of (1.53±0.35)%, and a perihelion shift of (−1.5±13.5) days. Published by the American Physical Society 2024

Staphylococcus aureus persistence in osteocytes: weathering the storm of antibiotics and autophagy/xenophagy.

Staphylococcus aureus is a major causative pathogen of osteomyelitis. Intracellular infections of resident bone cells including osteocytes can persist despite gold-standard clinical intervention. The mechanisms by which intracellular S. aureus evades antibiotic therapy are unknown. In this study, we utilised an in vitro S. aureus infection model of human osteocytes to investigate whether antibiotic-mediated dysregulation of autophagy contributes to this phenomenon. Infected or non-infected osteocyte-like cells were exposed to combinations of rifampicin, vancomycin, and modulators of autophagy. Intracellular bacterial growth characteristics were assessed using colony-forming unit (CFU) analysis, viable bacterial DNA abundance, and the rate of escape into antibiotic-free medium, together with measures of autophagic flux. Rifampicin, alone or in combination with vancomycin, caused a rapid decrease in the culturability of intracellular bacteria, concomitant with stable or increased absolute bacterial DNA levels. Both antibiotics significantly inhibited autophagic flux. However, modulation of autophagic flux did not affect viable bacterial DNA levels. In summary, autophagy was shown to be a factor in the host-pathogen relationship in this model, as its modulation affected the growth state of intracellular S. aureus with respect to both their culturability and propensity to escape the intracellular niche. While rifampicin and vancomycin treatments moderately suppressed autophagic flux acutely, this did not explain the paradoxical response of antibiotic treatment in decreasing S. aureus culturability whilst failing to clear bacterial DNA and hence intracellular bacterial load. Thus, off-target effects of rifampicin and vancomycin on autophagic flux in osteocyte-like cells could not explain the persistent S. aureus infection in these cells.

Direct measurements of sea spray particle fluxes using a high temporal resolution optical particle counter over the coastal ocean

ABSTRACT Sea spray particles play a key role in transferring momentum, heat, and gas across the atmosphere–ocean interface at high wind speeds and represent an important source of cloud condensation nuclei which affect the genesis, chemistry, and radiative properties of marine clouds. Here, we present direct measurements of sea spray particle fluxes obtained using an eddy covariance technique through the use of a newly developed high temporal resolution optical particle counter. With this instrumentation measurements were made over the coastal ocean during a 5-week field campaign conducted at an observation pier from November to December 2021. Our optical particle counter was capable of measuring size spectra at a rate of 10 Hz in 8 channels covering a range of mean radii between 0.3 and 15. The power spectra of particle number density followed the Kolmogorov −2/3 power law. The shape of the cospectrum of the particle number density flux was basically similar to that of the cospectra of the heat and gas fluxes. The measured sea spray particle fluxes at mean wind speeds of up to 21 m s−1 were dominated by an upward flux, which likely represents aerosol production caused by the bursting of bubbles at the ocean surface.

Thermo-osmotic coefficients in membrane distillation: Experiments and theory for three types of membranes

The thermo-osmotic coefficient of a porous medium is the ratio of the mass flow rate and the temperature difference that drives the flow. Thermo-osmotic coefficients of the Millipore DuraPore HVHP, GVHP and VVHP membranes have been measured using a new apparatus designed to provide accurate mass flux measurements with tightly controlled temperatures, pressures, and compositions on the feed and distillate side. To properly analyze the experimental data, recommendations on data reduction procedures anchored in non-equilibrium thermodynamics are discussed. An expression is presented for the apparent energy of activation of the transport process in terms of the derivative of the thermo-osmotic coefficient with respect to the inverse mean temperature. The expression is shown to accurately predict the temperature dependence of the thermo-osmotic coefficients from experiments. The expression is next used to explain observations in similar porous systems from the literature. Based on this, an optimization scheme to find the optimal mean pore radius to maximize the power density of a pressure-retarded membrane distillation (PRMD) process is presented. For membranes with properties similar to the Durapore XXHP series membranes, the optimal pore radius is found to be between 1 and 10 nm. It is shown that the power density can benefit greatly from a narrow pore size distribution and from membrane surface treatments that increase the liquid-solid contact angle, potentially exceeding a benchmark minimum power density of 5 W/m2 (set for the feasibility of pressure-retarded osmosis). An assessment of the second law efficiency of the PRMD process is also presented. A critical part of the process is due to the inevitably smaller water flux through smaller pores, required to maintain a high pressure difference and a feasible power density. This can only be alleviated by increasing the temperature difference across the membrane. We conclude that membranes designed for the PRMD process need increased overall thermal resistance to enhance performance.

Genetic Algorithm as the Solution of Non-Linear Inverse Heat Conduction Problems: A Novel Sequential Approach

Abstract Direct measurement of surface heat flux could be extremely challenging, if not impossible, in numerous applications. In such cases, the use of temperature measurement data from subsurface locations can facilitate the determination of surface heat flux and temperature through the solution of the inverse heat conduction problem (IHCP). Several different techniques have been developed over the years for solving IHCPs, with different levels of complexity and accuracy. The filter coefficient technique has proved to be a promising approach for solving IHCPs. Inspired by the filter coefficient approach, a novel method is presented in this paper for solving one-dimensional IHCPs in a domain with temperature-dependent material properties. A test case is developed in COMSOL Multiphysics where the front side of a slab is subject to known transient heat flux and the temperature distributions within the domain are calculated through numerical simulation. The IHCP solution in the form of filter coefficients is defined and a genetic algorithm (GA) is used for the calculation of filter matrix. The number of significant filter coefficients required to evaluate surface heat flux at each time-step is determined through trial and error and the optimal number is selected for evaluating the solution. The structure of the filter matrix is assessed and discussed. The resulting filter coefficients are then used to evaluate the surface heat flux for several different test cases and the performance of the proposed approach is assessed in detail. The results showed that the presented approach is robust and can result in finding optimal filter coefficients that can accurately estimate various types of surface heat flux profiles using temperature data from a limited number of time steps and in a near real-time fashion.

Radiative Heat Flux Measurement in a Semi-Industrial Oxyfuel Combustion Chamber with Biomass and Coal

Oxyfuel is a combustion technology where the oxidant consists mainly of oxygen and carbon dioxide instead of oxygen and nitrogen. Since carbon dioxide has strongly absorbing bands in the thermal spectrum, the radiation properties of the flame change in an oxyfuel atmosphere compared to conventional combustion. When retrofitting an existing air-fired combustion system to an oxyfuel process, the oxygen content in the oxidant must be adjusted so that similar values for heat transfer by radiation are achieved. This measure allows the system to be operated with otherwise unchanged parameters. In this work, the thermal radiation of natural gas, pulverised walnut shells and lignite under an air and oxyfuel atmosphere is investigated in a semi-industrial combustion chamber with water-cooled membrane walls, at different oxygen concentrations and combustion parameters. While the radiative heat fluxes for natural gas with an oxygen content of 28 vol% in the oxidant are significantly higher than those for firing with air, the values for lignite are still below the air-firing, even with an oxygen content of 30 vol%. For walnut shells, the oxyfuel results are close to the air case for all oxygen concentrations between 27 and 33 vol%. The walnut shells show higher radiative emissions than the lignite at the same thermal output. For non-swirled flames, the radiative heat flux is lower than for swirled flames.

Alder expansion stimulates nitrogen oxide (NOx) emissions from southern Eurasian permafrost peatlands

AbstractNitrogen oxides (NOx) play an important role for atmospheric chemistry and radiative forcing. However, NOx emissions from the vast northern circumpolar permafrost regions have not been studied in situ due to limitations of measurement techniques. Our goals were to validate the offline analytical technique, and based on this, to widely quantify in situ NOx emissions from peatlands in the southern Eurasian permafrost region. To this end, we conducted a comparison of online and offline flux measurements in 2018 and 2019 using the synthetic air flushing, steady‐state opaque chamber method. With differences in annual average and cumulative fluxes less than 0.1 μg N m−2 h−1 and 0.01 kg N ha−1 year−1, the online and offline fluxes were in good agreement, demonstrating the feasibility of conducting offline measurements in remote regions without power supply. The flux measurements over 2 years showed obvious NOx emissions of 0.05–0.14 and 0.13–0.30 kg N ha−1 year−1 in the hollow and hummock microtopography of permafrost peatlands, respectively. The rapid expansion of alder (Alnus sibirica) in the peatlands induced by permafrost degradation significantly increased soil mineral N contents and NOx emissions depending on the age of alder (0.64–1.74 and 1.44–2.20 kg N ha−1 year−1 from the alder forests with tree ages of 1–10 years and 11–20 years, respectively). Alder expansion also intensively altered the thermal state of permafrost including the sharp increases of soil temperatures during the non‐growing season from October to April and active layer thickness. This study provides the first in situ evidences of NOx emissions from the northern circumpolar permafrost regions and uncovers the well‐documented expansion of alders can substantially stimulate NOx emissions and thus, significantly affect air quality, radiative forcing, and ecosystem productivity in the pristine regions.

Microbial Ecology and Site Characteristics Underlie Differences in Salinity‐Methane Relationships in Coastal Wetlands

AbstractMethane (CH4) is a potent greenhouse gas emitted by archaea in anaerobic environments such as wetland soils. Tidal freshwater wetlands are predicted to become increasingly saline as sea levels rise due to climate change. Previous work has shown that increases in salinity generally decrease CH4 emissions, but with considerable variation, including instances where salinization increased CH4 flux. We measured microbial community composition, biogeochemistry, and CH4 flux from field samples and lab experiments from four different sites across a wide geographic range. We sought to assess how site differences and microbial ecology affect how CH4 emissions are influenced by salinization. CH4 flux was generally, but not always, positively correlated with CO2 flux, soil carbon, ammonium, phosphate, and pH. Methanogen guilds were positively correlated with CH4 flux across all sites, while methanotroph guilds were both positively and negatively correlated with CH4 depending on site. There was mixed support for negative relationships between CH4 fluxes and concentrations of alternative electron acceptors and abundances of taxa that reduce them. CH4/salinity relationships ranged from negative, to neutral, to positive and appeared to be influenced by site characteristics such as pH and plant composition, which also likely contributed to site differences in microbial communities. The activity of site‐specific microbes that may respond differently to low‐level salinity increases is likely an important driver of CH4/salinity relationships. Our results suggest several factors that make it difficult to generalize CH4/salinity relationships and highlight the need for paired microbial and flux measurements across a broader range of sites.