Flux Redistribution Research Articles

Control of Networked Systems by Clustering: The Degree of Freedom Concept

We address the problem of local flux redistribution in networked systems. The aim is to detect a suitable cluster which is able to locally adsorb a disturbance by means of an appropriate redistribution of control load among its nodes, such that no external node is affected. Traditional clustering measures are not suitable for our purpose, since they do not explicitly take into account the structural conditions for disturbance containment. We propose a new measure based on the concept of degree of freedom for a cluster, and we introduce a heuristic procedure to quickly select a set of nodes according to this measure. Finally, we show an application of the method in the context of DC microgrids voltage control.

Usage of multiple fission cells for neutron flux measurements during rod-insertion method

The measurements of physical parameters of the TRIGA reactor and Nuclear power plant Krško (NEK) reactor cores have been in the past performed on hand of the neutron flux signal obtained from uncompensated ionization cells and by employment of the a digital meter of reactivity (DMR). At the TRIGA reactor only one ionization cell is currently used for flux measurements. During the insertion of one control rod the neutron flux distribution is significantly altered affecting the flux measurements of inserting different control rods. The problem is presently solved by assigning a correction factor to each control rod what introduces an additional uncertainty. In the present paper the implementation of four fission cells for reactivity measurements is presented. In this way determining the correct gamma background and its subtraction, performed by DMR algorithms, becomes less important as previously by using ionization chambers. The larger number of detectors also reduces the flux redistribution effects on the signal during individual control rod movements.

Enhanced Production of 5-aminolevulinic Acid via Flux Redistribution of TCA Cycle toward l-Glutamate in Corynebacterium glutamicum

5-Aminolevulinic acid (ALA), a valuable nonproteinogenic amino acid, has received increasing attention in various fields including medicine, agriculture, and cosmetics. Here, we developed metabolically engineered Corynebacterium glutamicum to enhance ALA production. To achieve this object, we focused on the flux redistribution of the TCA cycle toward l-glutamate and introduction of the heterogenous ALA transporter in C. glutamicum. First, the oxoglutarate dehydrogenase inhibitor (OdhI) was mutated by site-directed mutagenesis to prevent the phosphorylation that abolishes the capability of OdhI protein to inhibit oxoglutarate dehydrogenase complex activity. The overexpression of the double-mutated OdhI, T14A/T15A, showed the highest l-glutamate and ALA production compared with that of the native and single-mutated OdhI. To increase ALA secretion from the engineered strain, the ALA exporter RhtA from Escherichia coli was introduced and allowed 2.46 ± 0.11 g/L of ALA production, representing a 1.28-fold increase in extracellular ALA production. In the final strain, the induction of triggers, including Tween 40 and ethambutol, was performed to amplify the effect of the flux redistribution toward ALA. A significant increase in ALA production was observed in the induction of triggers. In particular, ethambutol induction showed the best result, corresponding to 2.9 ± 0.15 g/L of ALA production. Therefore, this biotechnological model enables the efficient extracellular production of ALA from glucose in C. glutamicum.

Signatures of Nonideal Plasma Evolution During Substorms Obtained by Mining Multimission Magnetometer Data

AbstractSubstorm‐type evolution of the Earth's magnetosphere is investigated by mining more than two decades (1995–2017) of spaceborne magnetometer data from multiple missions including the first two years (2016‐2017) of the Magnetospheric MultiScale mission. This investigation reveals interesting features of plasma evolution distinct from ideal magnetohydrodynamics (MHD) behavior: X‐lines, thin current sheets, and regions with the tailward gradient of the equatorial magnetic field Bz. X‐lines are found to form mainly beyond 20 RE, but for strong driving, with the solar wind electric field exceeding ∼5mV/m, they may come closer. For substorms with weaker driving, X‐lines may be preceded by redistribution of the magnetic flux in the tailward Bz gradient regions, similar to the magnetic flux release instability discovered earlier in PIC and MHD simulations as a precursor mechanism of the reconnection onset. Current sheets in the growth phase may be as thin as 0.2 RE, comparable to the thermal ions gyroradius, and at the same time, as long as 15 RE. Such an aspect ratio is inconsistent with the isotropic force balance for observed magnetic field configurations. These findings can help resolve kinetic mechanisms of substorm dipolarizations and adjust kinetic generalizations of global MHD models of the magnetosphere. They can also guide and complement microscale analysis of nonideal effects.

Composite fermion state of graphene as a Haldane-Chern insulator

Graphene in the presence of a strong external magnetic field is a unique attraction for investigations of the fractional quantum Hall (fQH) states with odd and even denominators of the fraction. Most of the attempts to understand Graphene in the strong-field regime were made through exploiting the universal low-energy effective description of Dirac fermions emerging from the nearest neighbor hopping model of electrons on a honeycomb lattice. We highlight that accounting for the next-nearest-neighbor hopping terms in doped Graphene can lead to a unique redistribution of magnetic fluxes within the unit cell of the lattice. While this affects all the fQH states, it has a striking effect at a half-filled Landau-level state: it leads to a composite fermion state that is equivalent to the doped topological Chern insulator on a honeycomb lattice. At energies comparable to the Fermi energy, this state possesses a Haldane gap in the bulk proportional to the next-nearest-neighbor hopping and density of dopants. We argue that this microscopically derived energy gap survives the projection to the lowest band. We also conjecture that the gap should be present in a microscopic theory giving the recently proposed particle-hole symmetric Dirac composite fermion scenario of the half-filled Landau-level. The proposed gap is lower than the chemical potential, and is predicted to be parametrically separated from the Dirac point in the latter description. Finally we conclude by proposing experiments to detect this gap; the associated boundary mode; and encourage cold-atom setups to test other predictions of the theory.

Disc formation in magnetized dense cores with turbulence and ambipolar diffusion

ABSTRACT Discs are essential to the formation of both stars and planets, but how they form in magnetized molecular cloud cores remains debated. This work focuses on how the disc formation is affected by turbulence and ambipolar diffusion (AD), both separately and in combination, with an emphasis on the protostellar mass accretion phase of star formation. We find that a relatively strong, sonic turbulence on the core scale strongly warps but does not completely disrupt the well-known magnetically induced flattened pseudo-disc that dominates the inner protostellar accretion flow in the laminar case, in agreement with previous work. The turbulence enables the formation of a relatively large disc at early times with or without AD, but such a disc remains strongly magnetized and does not persist to the end of our simulation unless a relatively strong AD is also present. The AD-enabled discs in laminar simulations tend to fragment gravitationally. The disc fragmentation is suppressed by initial turbulence. The AD facilitates the disc formation and survival by reducing the field strength in the circumstellar region through magnetic flux redistribution and by making the field lines there less pinched azimuthally, especially at late times. We conclude that turbulence and AD complement each other in promoting disc formation. The discs formed in our simulations inherit a rather strong magnetic field from its parental core, with a typical plasma-β of order a few tens or smaller, which is 2–3 orders of magnitude lower than the values commonly adopted in magnetohydrodynamic simulations of protoplanetary discs. To resolve this potential tension, longer term simulations of disc formation and evolution with increasingly more realistic physics are needed.

Asymmetric heating and buoyancy effects on heat transfer of hydrocarbon fuel in a horizontal square channel at supercritical pressures

Heat transfer of the hydrocarbon fuel at a supercritical pressure is a key process in the aerospace engine cooling applications. A CFD model is applied in this paper to study flow dynamics and heat transfer of the aviation kerosene RP-3 in a horizontal square cooling channel under asymmetric heating and buoyancy effects at various supercritical pressures. The turbulent fluid flows are handled by the standard k–ε turbulence model with an enhanced wall treatment, and the strong thermophysical property variations are calculated using the extended corresponding state approaches and a four-component surrogate model of RP-3. The effects of secondary flows and heat flux redistribution induced by buoyancy on supercritical-pressure heat transfer are analyzed. Results indicate that drastic variations of the fuel density with temperature at a supercritical pressure of 3 MPa induce strong buoyancy effect on heat transfer. In the top heated case, secondary flows carry the relatively cold fluid to the side and opposite walls, thereby increasing their fin effectiveness in heat transfer. As the operating pressure increases from 3 to 5 MPa, the density variation of RP-3 is decreased, consequently leading to the weakened buoyancy effect. As the solid thermal conductivity increases from 20 to 100 W/(m-K), the surface heat flux redistribution is dictated by heat conduction in the channel walls, and therefore, the buoyancy effect is significantly reduced. Numerical results herein could help elucidate the heat transfer characteristics of RP-3 in practical engine cooling processes.

Programmable biomolecular switches for rewiring flux in Escherichia coli

Synthetic biology aims to develop programmable tools to perform complex functions such as redistributing metabolic flux in industrial microorganisms. However, development of protein-level circuits is limited by availability of designable, orthogonal, and composable tools. Here, with the aid of engineered viral proteases and proteolytic signals, we build two sets of controllable protein units, which can be rationally configured to three tools. Using a protease-based dynamic regulation circuit to fine-tune metabolic flow, we achieve 12.63 g L−1 shikimate titer in minimal medium without inducer. In addition, the carbon catabolite repression is alleviated by protease-based inverter-mediated flux redistribution under multiple carbon sources. By coordinating reaction rate using a protease-based oscillator in E. coli, we achieve d-xylonate productivity of 7.12 g L−1 h−1 with a titer of 199.44 g L−1. These results highlight the applicability of programmable protein switches to metabolic engineering for valuable chemicals production.

Kinetic model optimization and its application to mitigating the Warburg effect through multiple enzyme alterations

Pathway engineering is a powerful tool in biotechnological and clinical applications. However, many phenomena cannot be rewired with a single enzyme change, and in a complex network like energy metabolism, the selection of combinations of targets to engineer is a daunting task. To facilitate this process, we have developed an optimization framework and applied it to a mechanistic kinetic model of energy metabolism. We then identified combinations of enzyme alternations that led to the elimination of the Warburg effect seen in the metabolism of cancer cells and cell lines, a phenomenon coupling rapid proliferation to lactate production. Typically, optimization approaches use integer variables to achieve the desired flux redistribution with a minimum number of altered genes. This framework uses convex penalty terms to replace these integer variables and improve computational tractability. Optimal solutions are identified which substantially reduce or eliminate lactate production while maintaining the requirements for cellular proliferation using three or more enzymes.

Effect of the Temperature on the Redistribution of an Energy Flux Carried by Surface Waves along the Interface between Crystals with Different Mechanisms of Formation of a Nonlinear Response

A model of an interface between a uniaxial crystal with the diffusion mechanism of formation of a nonlinear photorefractive effect and crystals with both focusing and defocusing Kerr nonlinearities has been considered. New types of transverse magnetic polarized nonlinear surface waves propagating along the interface between crystals have been revealed. These types of waves have different ranges of existence and different characters of damping of the field with increasing distance from the interface. Energy fluxes carried by such surface waves have been determined. It has been shown that, as the temperature of crystals near the interface is varied, the radiation power carried by nonlinear surface waves is redistributed between adjacent crystals.

HST/STIS analysis of the first main sequence pulsar CU Virginis

Context. CU Vir has been the first main sequence star that showed regular radio pulses that persist for decades, resembling the radio lighthouse of pulsars and interpreted as auroral radio emission similar to that found in planets. The star belongs to a rare group of magnetic chemically peculiar stars with variable rotational period. Aims. We study the ultraviolet (UV) spectrum of CU Vir obtained using STIS spectrograph onboard the Hubble Space Telescope (HST) to search for the source of radio emission and to test the model of the rotational period evolution. Methods. We used our own far-UV and visual photometric observations supplemented with the archival data to improve the parameters of the quasisinusoidal long-term variations of the rotational period. We predict the flux variations of CU Vir from surface abundance maps and compare these variations with UV flux distribution. We searched for wind, auroral, and interstellar lines in the spectra. Results. The UV and visual light curves display the same long-term period variations supporting their common origin. New updated abundance maps provide better agreement with the observed flux distribution. The upper limit of the wind mass-loss rate is about 10−12 M⊙ yr−1. We do not find any auroral lines. We find rotationally modulated variability of interstellar lines, which is most likely of instrumental origin. Conclusions. Our analysis supports the flux redistribution from far-UV to near-UV and visual domains originating in surface abundance spots as the main cause of the flux variability in chemically peculiar stars. Therefore, UV and optical variations are related and the structures leading to these variations are rigidly confined to the stellar surface. The radio emission of CU Vir is most likely powered by a very weak presumably purely metallic wind, which leaves no imprint in spectra.

Impact of acetolactate synthase inactivation on 1,3-propanediol fermentation by Klebsiella pneumoniae.

1,3-Propanediol (1,3-PDO) is an important compound that is mainly used in industry for polymer production. Fermentation of 1,3-PDO from glycerol by Klebsiella pneumoniae is accompanied by formation of 2,3-butanediol (2,3-BDO) as one of the main byproduct. The first step in the formation of 2,3-BDO from pyruvate is catalyzed by acetolactate synthase (ALS), an enzyme that competes with 1,3-PDO oxidoreductase for the cofactor NADH. This study aimed to analyze the impact of engineering the 2,3-BDO formation pathway via inactivation of ALS on 1,3-PDO fermentation by K. pneumoniae HSL4. An ALS mutant was generated using Red recombinase assisted gene replacement. The ALS specific activities of K. pneumoniae ΔALS were notably lower than that of the wild-type strain. Fed-batch fermentation of the mutant strain resulted in a 1,3-PDO concentration, productivity and conversion of 72.04 g L–1, 2.25 g L–1 h–1, and 0.41 g g–1, increase by 4.71%, 4.65% and 1.99% compared with the parent strain. Moreover, inactivation of ALS decreased meso-2,3-BDO formation to trace amounts, significantly increased 2S,3S-BDO and lactate production, and a pronounced redistribution of intracellular metabolic flux was apparent.

Metabolic and physiological perturbations of Escherichia coli W3100 by bacterial small RNA RyhB

RyhB is a key regulator of iron level in Escherichia coli (E. coli), which assists in conserving iron for life-sustaining cellular functions when cytoplasmic levels of the ferrous form of iron is limited. RyhB affects glucose metabolism. Seventy percent of the genes that are regulated by RyhB are related to metabolism. We demonstrated for the first time that the activity of the pentose phosphate pathway increased upon ryhB activation using a13C stable isotope-based technique called METAFoR (Metabolic flux ratio analysis). U-13C glucose-based studies showed that the reversible exchange activity of serine and glycine was enhanced by flux redistribution, which further favors NADPH formation. In addition, Entner–Doudoroff (ED) pathway activity was inhibited in the ryhB-defective cells. Quantitative physiology-based experiments highlighted a significant increase in the levels of reactive oxygen species (ROS) in ryhB-induced W3100 E. coli cells in batch culture. A simultaneous decrease in NADH/NAD+ and NADPH/NADP+ ratios outlined the potentially direct roles of NADH and NADPH in antagonizing the excess ROS formed after ryhB activation. Our observations offer a new perspective regarding the roles of RyhB and highlight that this small RNA can significantly affect cell metabolism in addition to its role as a regulator of gene expression.

RNA accumulation in Candida tropicalis based on cofactor engineering.

Redox cofactors play an important role in biosynthetic and catabolic reactions and the transfer of energy for the cell. Therefore, studying the relationship between cofactor perturbation and metabolism is a useful approach to improve the yield of target products. To study RNA accumulation and metabolism when intracellular cofactor balance was impaired, the water-forming NADH oxidase (NoxE) from Lactococcus lactis and membrane-bound transhydrogenase (PntAB) from Escherichia coli were expressed in Candidatropicalis no. 121, respectively. Expression of noxE significantly decreased the intracellular NADH/NAD+ ratio, but the NADPH/NADP+ ratio did not differ significantly. PntAB increased the intracellular NADH pool, while the NADPH/NADP+ ratio decreased. The perturbation of the cofactors caused a large redistribution of metabolic fluxes. The biomass and RNA content decreased by 11.0% and 10.6% in pAUR-noxE strain, respectively, while the RNA content increased by 5.5% and the biomass showed no signification difference in pAUR-pntAB strain. Expression of noxE and pntAB led to decreases and increases in the ATP concentration and yield of RNA, respectively, which also indicated that ATP plays an important role in the RNA biosynthesis.

Influence of gibberellin on the mesostructural organization of the leaf, accumulation and redistribution of assimilates and feeding elements of the gooseberry plants (Grossularia reclinat) in connection with the productivity of culture

The effect of gibberellic acid on morphogenesis, mesostructural leaf organization, accumulation and redistribution of various forms of carbohydrates, nitrogen, phosphorus and potassium between the organs of gooseberry plants of the Mashenka variety in relation to the productivity of the culture has been established. The aim of the study was to find out the peculiarities of morphogenesis, the formation of the leaf apparatus, the accumulation and redistribution of assimilates and nitrogen-containing compounds between the organs of gooseberry plants for the actions of gibberellic acid in connection with the productivity of the culture. Materials and methods. Plants were treated once during the budding period with a 0.05 % m gibireleic acid solution during the budding phase. The carbohydrate content was determined by the iodometric method, nitrogen - by Keldahl, phosphorus - by the intensity of the formation of the phosphorus-molifbdenovogo complex, potassium - by flame-photometric, the content of chlorophylls - spectrophotometrically. Results . Under the influence of the drug, a more powerful donor sphere was formed due to the growth of chlorenchyma due to an increase in the volume and linear dimensions of the cells of the columnar and spongy assimilation tissue. This led to enhanced assimilation, an increase in the content of non-structural carbohydrates (sugar + starch) in the shoots. Processing gibberellin contributed to the optimization of nitrogen, phosphorus and potassium in the leaves and shoots. A significant deponuvalous capacity of the stems in the temporary redundancy of an aksimilit and gooseberry plant nutrients with the subsequent reutilization of them to the processes of carpogenesis has been established. Changes in the nature of donor-acceptor relations in gooseberry plants under the influence of gibberellic acid caused the redistribution of assimilate and nutrient fluxes from vegetative organs to fruits, and had a positive effect on the crop productivity. Findings. The use of gibberellic acid led to the formation of a more powerful donor sphere, the accumulation and temporary deposit of the part of carbohydrates and mineral nutrients in the vegetative organs of gooseberry plants, followed by their remobilization on the need for fruit formation and growth, led to a significant increase in yield and improved the product quality

13C-Metabolic Flux Analysis Reveals the Metabolic Flux Redistribution for Enhanced Production of Poly-γ-Glutamic Acid in dlt Over-Expressed Bacillus licheniformis.

Poly-γ-glutamic acid (γ-PGA) is an anionic polymer with various applications. Teichoic acid (TA) is a special component of cell wall in gram-positive bacteria, and its D-alanylation modification can change the net negative charge of cell surface, autolysin activity and cationic binding efficiency, and might further affect metabolic production. In this research, four genes (dltA, dltB, dltC, and dltD) of dlt operon were, respectively, deleted and overexpressed in the γ-PGA producing strain Bacillus licheniformis WX-02. Our results implied that overexpression of these genes could all significantly increase γ-PGA synthetic capabilities, among these strains, the dltB overexpression strain WX-02/pHY-dltB owned the highest γ-PGA yield (2.54 g/L), which was 93.42% higher than that of the control strain WX-02/pHY300 (1.31 g/L). While, the gene deletion strains produced lower γ-PGA titers. Furthermore, 13C-Metabolic flux analysis was conducted to investigate the influence of dltB overexpression on metabolic flux redistribution during γ-PGA synthesis. The simulation data demonstrated that fluxes of pentose phosphate pathway and tricarboxylic acid cycle in WX-02/pHY-dltB were 36.41 and 19.18 mmol/g DCW/h, increased by 7.82 and 38.38% compared to WX-02/pHY300 (33.77 and 13.86 mmol/g DCW/h), respectively. The synthetic capabilities of ATP and NADPH were also increased slightly. Meanwhile, the fluxes of glycolytic and by-product synthetic pathways were all reduced in WX-02/pHY-dltB. All these above phenomenons were beneficial for γ-PGA synthesis. Collectively, this study clarified that overexpression of dltB strengthened the fluxes of PPP pathway, TCA cycle and energy metabolism for γ-PGA synthesis, and provided an effective strategy for enhanced production of γ-PGA.

Chromomycin, an antibiotic produced by Streptomyces flaviscleroticus might play a role in the resistance to oxidative stress and is essential for viability in stationary phase.

The well-known role of antibiotics in killing sensitive organisms has been challenged by the effects they exert at subinhibitory concentrations. Unfortunately, there are very few published reports on the advantages these molecules may confer to their producers. This study describes the construction of a genetically verified deletion mutant of Streptomyces flaviscleroticus unable to synthesize chromomycin. This mutant was characterized by a rapid loss of viability in stationary phase that was correlated with high oxidative stress and altered antioxidant defences. Altered levels of key metabolites in the mutant signalled a redistribution of the glycolytic flux toward the PPP to generate NADPH to fight oxidative stress as well as reduction of ATP-phosphofructokinase and Krebs cycle enzymes activities. These changes were correlated with a shift in the preference for carbon utilization from glucose to amino acids. Remarkably, chromomycin at subinhibitory concentration increased longevity of the non-producer and restored most of the phenotypic features' characteristic of the wild type strain. Altogether these observations suggest that chromomycin may have antioxidant properties that would explain, at least in part, some of the phenotypes of the mutant. Our observations warrant reconsideration of the secondary metabolite definition and raise the possibility of crucial roles for their producers.

An upper limit on Gibbs energy dissipation governs cellular metabolism.

The principles governing cellular metabolic operation are poorly understood. Because diverse organisms show similar metabolic flux patterns, we hypothesized that a fundamental thermodynamic constraint might shape cellular metabolism. Here, we develop a constraint-based model for Saccharomyces cerevisiae with a comprehensive description of biochemical thermodynamics including a Gibbs energy balance. Non-linear regression analyses of quantitative metabolome and physiology data reveal the existence of an upper rate limit for cellular Gibbs energy dissipation. By applying this limit in flux balance analyses with growth maximization as the objective function, our model correctly predicts the physiology and intracellular metabolic fluxes for different glucose uptake rates as well as the maximal growth rate. We find that cells arrange their intracellular metabolic fluxes in such a way that, with increasing glucose uptake rates, they can accomplish optimal growth rates but stay below the critical rate limit on Gibbs energy dissipation. Once all possibilities for intracellular flux redistribution are exhausted, cells reach their maximal growth rate. This principle also holds for Escherichia coli and different carbon sources. Our work proposes that metabolic reaction stoichiometry, a limit on the cellular Gibbs energy dissipation rate, and the objective of growth maximization shape metabolism across organisms and conditions.

Evolution of Asymmetrically Displaced Footpoints During Substorms

AbstractIt is well established that a transverse (y) component in the interplanetary magnetic field (IMF) induces a By component in the closed magnetosphere through asymmetric loading and/or redistribution of magnetic flux. Simultaneous images of the aurora in the two hemispheres have revealed that conjugate auroral features are displaced longitudinally during such conditions. Although the direction and magnitude of this displacement show correlations with IMF clock angle and dipole tilt, single events show large temporal and spatial variability of this displacement. For instance, we know little about how the displacement changes during a substorm. A previous case study demonstrated that displaced auroral forms, associated with the prevailing IMF orientation, returned to a more symmetric configuration during the expansion phase of two substorms. Using the far ultraviolet cameras on board the Imager for Magnetopause‐to‐Aurora Global Exploration and Polar satellites, we have identified multiple events where conjugate auroral images are available during periods with substorm activity and IMF By≠0. We identify conjugate auroral features and investigate how the asymmetry evolves during the expansion phase. We find that the system returns to a more symmetric state in the events with a clear increase in the nightside reconnection rate and that the displacement remains unchanged in the events with little or no net closure of open magnetic flux. The return to a more symmetric state can therefore be interpreted as the result of increased reconnection rate in the magnetotail during the expansion phase, which reduces the asymmetric lobe pressure.

Experimental study on the magnetic coherent mode in the H-mode pedestal of EAST

Here we report the experimental analysis on the low-n (mostly n = 1, sometimes n = 2) magnetic coherent mode (MCM) at a characteristic frequency 20–, which has been frequently observed in various H-mode discharges on EAST. This mode can be easily identified in the magnetic fluctuations measured by the fast Mirnov coils mounted on the vacuum vessel wall, but is detected by the local measurements of edge electrostatic fluctuations only when the mode is sufficiently strong. The apperance of the MCM is summarized covering broad ranges of discharge parameters, in particular, the different heating schemes including pure neutral beams injected in either co- or counter-current direction as well as pure ratio-frequency waves. This may rule out the possibility of fast particle driven modes. Radial distribution and poloidal propagation of the MCM are investigated using the Doppler backscattering system and Langmuir probes inserted at the outer midplane, respectively. Temporal evolution of MCM amplitude during large ELM crashes is evaluated in detail, may suggesting the mode is closely correlated with pedestal buildup. Dedicated experiments reveal the possible correlations of MCM’s frequencies with edge line-averaged density and edge safety factor q95. We also present the observation of multi MCMs at relatively high q95, which are speculated locating at different rational surfaces in the pedestal via analyzing their mode structures and nonlinear interactions. Finally, effect of the MCM on edge particle transport is explored via surveying the correlation between the intermittent events of the mode and the particle fluxes deposited on the divertor target plates, utilizing the conditional analysis method. Corresponding results suggest that the MCM seems to primarily result in a notable poloidal redistribution of the divertor particle flux, rather than a considerable net increase of the total flux.