Fluorescent Metal-organic Framework Research Articles

Recent Progress in Metal-Organic Framework Based Fluorescent Sensors for Hazardous Materials Detection.

Population growth and industrial development have exacerbated environmental pollution of both land and aquatic environments with toxic and harmful materials. Luminescence-based chemical sensors crafted for specific hazardous substances operate on host-guest interactions, leading to the detection of target molecules down to the nanomolar range. Particularly, the luminescence-based sensors constructed on the basis of metal-organic frameworks (MOFs) are of increasing interest, as they can not only compensate for the shortcomings of traditional detection techniques, but also can provide more sensitive detection for analytes. Recent years have seen MOFs-based fluorescent sensors show outstanding advantages in the field of hazardous substance identification and detection. Here, we critically discuss the application of MOFs for the detection of a broad scope of hazardous substances, including hazardous gases, heavy metal ions, radioactive ions, antibiotics, pesticides, nitro-explosives, and some harmful solvents as well as luminous and sensing mechanisms of MOF-based fluorescent sensors. The outlook and several crucial issues of this area are also discussed, with the expectation that it may help arouse widespread attention on exploring fluorescent MOFs (LMOFs) in potential sensing applications.

Cover Feature: Unfolding the Role of Building Units of MOFs with Mechanistic Insight Towards Selective Metal Ions Detection in Water (Chem. Eur. J. 21/2022)

The role of the organic building units of MOFs towards selective detection of a targeted metal cation (Fe3+) has been demonstrated by systematic screening by varying the number of Lewis basic (pyridyl-N atoms) sites in a series of isostructural metal–organic frameworks (MOFs). All three fluorescent MOFs are seen to present a quenching response towards Fe3+ ions in water; however, only UiO-67@N exhibits highly selective and sensitive response, whereas the emission of both UiO-67 and UiO-67@NN is quenched by several metal ions. More information can be found in the Research Article by S. K. Ghosh et al. (DOI: 10.1002/chem.202104175).

Unfolding the Role of Building Units of MOFs with Mechanistic Insight Towards Selective Metal Ions Detection in Water.

The potential emergence of fluorescence-based techniques has propelled research towards developing probes that can sense trace metal ions specifically. Although luminescent metal-organic frameworks (MOFs) are well suited for this application, the role of building blocks towards detection is not fully understood. In this work, a systematic screening by varying number of Lewis basic (pyridyl-N atoms) sites is carried out in a series of isostructural, robust UiO-67 MOFs, and targeting a model metal ion-Fe3+ . All the three fluorescent MOFs are seen to present quenching response towards Fe3+ ions in water. However, UiO-67@N exhibits highly selective and sensitive response, whereas emission of both UiO-67 and UiO-67@NN is quenched by several metal ions. Detailed experimental and theoretical mechanistic investigation is carried out in addition to demonstration of UiO-67@N being able to sense trace amount of Fe3+ ions in synthetic biological water sample. Further, UiO-67@N based mixed-matrix membrane (MMM) has been prepared and employed to mimic the real time Fe3+ ions detection in water.

Multifunctional luminescent switch based on a porous PL-MOF for sensitivity recognition of HCl, trace water and lead ion

The development of multifunctional luminescent switch for the detection of different targets is highly desirable because of the complexity of the environment. In this paper, novel porous fluorescent Metal–Organic Framework (HPU-23), which can visually detect gaseous hydrogen chloride (HCl) and trace water from organic solvents through the fluorescence quenching phenomena, are designed. Besides, after the fluorescence of HPU-23 quenching by HCl, HPU-23 can be reopened by gaseous triethylamine (TEA) treatment. HPU-23 also could detect trace water from organic solvents. Although the quenching of fluorescence is also observed, the sensor after sensing H2O cannot be regenerated by any treatment. Besides, another Pb2+ sensor is obtained when a new luminophore, i.e., tris(2,2′-bipyridine) dichlororuthenium(II) hexahydrate (Ru(bpy)32+), is added into an aqueous system containing HPU-23. Therefore, our strategy is a promising broadcast for the development of multifunctional sensors.

Fluorescent Lanthanide Metal-Organic Framework for Rapid and Ultrasensitive Detection of Methcathinone in Human Urine

Fluorescent Lanthanide Metal-Organic Framework for Rapid and Ultrasensitive Detection of Methcathinone in Human Urine

Ultrasensitive lateral flow immunoassay for staphylococcal enterotoxin B using nanosized fluorescent metal-organic frameworks.

Owing to their outstanding optical properties and superior physical/chemical stability, dye-doped fluorescent nanoparticles (NPs) are growing exponentially as signal labels of immunochromatographic lateral flow immunoassay (LFA) for the detection of various analytes. However, the key challenge in the design of these fluorescent NPs is to confine the fluorophores inside NPs at extreme concentrations, at which dyes tend to self-quench resulting from the formation of non-fluorescent aggregates. Looking for other advantageous nanomaterials, we propose for the first time the use of a nanosized fluorescent metal-organic framework (nanoMOF) in LFA for the detection of staphylococcal enterotoxin B (SEB) as a model analyte. Featured by the chromophore assembly, the nanoMOF exhibited a high dye loading (∼60%) and strong fluorescence intensity, which was due to the reduced self-quenching of dyes in a variety of MOF matrices. The strong green fluorescence intensity of the nanoMOF gives a high contrast against the background of the strips and the sensitivity reflected by photoluminescence was improved by the enhanced antenna effect. Furthermore, due to the high surface area for antibody stemming, the limit of detection (LOD) of the MOF based LFA for SEB detection was as low as 0.025 ng mL-1. The compatibility of the MOF based LFA with dairy samples and its stability under long-term storage conditions were also demonstrated. The integration of a nanoMOF into LFA to detect toxins could inspire the utilization of such nanomaterial-based labels in similar immunochromatographic testing methods to improve their performance.

Ultrafast Aggregation-Induced Tunable Emission Enhancement in a Benzothiadiazole-Based Fluorescent Metal-Organic Framework Linker.

Aggregation-induced emission enhancement (AIEE) is a process recently exploited in solid-state materials and organic luminophores, and it is explained by tight-molecular packaging. However, solution-phase AIEE and its formation mechanism have not been widely explored. This work investigated AIEE phenomena in two donor-acceptor-donor-type benzodiazole-based molecules (the organic building block in metal-organic frameworks) with an acetylene and phenyl π-conjugated backbone tapered with a carboxylic acid group at either end. This was done using time-resolved electronic and vibrational spectroscopy in conjunction with time-dependent density functional theory (TD-DFT) calculations. Fluorescence up-conversion spectroscopy and time-correlated single-photon counting conclusively showed an intramolecular charge transfer-driven aggregate emission enhancement. This is shown by a red spectral shift of the emission spectra as well as an increase in the fluorescence lifetime from 746 ps at 1.0 × 10-11 to 2.48 ns at 2.0 × 10-3 M. The TD-DFT calculations showed that a restricted intramolecular rotation mechanism is responsible for the enhanced emission. The femtosecond infrared (IR) transient absorption results directly revealed the structural dynamics of aggregate formation, as evident from the evolution of the C≡C vibrational marker mode of the acetylene unit upon photoexcitation. Moreover, the IR data clearly indicated that the aggregation process occurred over a time scale of 10 ps, which is consistent with the fluorescence up-conversion results. Interestingly, time-resolved results and DFT calculations clearly demonstrated that both acetylene bonds and the sulfur atom are the key requirements to achieve such a controllable aggregation-induced fluorescence enhancement. The finding of the work not only shows how slight changes in the chemical structure of fluorescent chromophores could make a tremendous change in their optical behavior but also prompts a surge of research into a profound understanding of the mechanistic origins of this phenomenon. This may lead to the discovery of new chemical strategies that aim to synthesize novel chromophores with excellent optical properties for light-harvesting applications.

Construction of a Luminescent Cadmium-Based Metal-Organic Framework for Highly Selective Discrimination of Ferric Ions.

Fluorescent metal–organic frameworks (MOFs) are ideal materials for sensors because of their adjustable pore size and functional groups, which provide them with favorable metal ion selective recognition. In this paper, a new cadmium-based MOF was synthesized using Cd(NO3)2·4H2O and 3,3′,5,5′-biphenyltetracarboxylic acid by solvothermal method. CdBPTC owned three types of channels with dimensions of approximately 8.4 × 8.3 Å, 6.0 × 5.2 Å, 9.7 × 8.4 Å along a, b, and c axis, respectively. This MOF has high selectivity to ferric ions and shows excellent anti-inference ability toward many other cations. The results indicate that the fluorescence quenching efficiency of CdBPTC is close to 100% when the concentration of Fe3+ reaches 1.0 × 10−3 mol·L−1. Moreover, the luminescent intensity at 427 nm presents a linear relationship at a concentration range of 2.0 × 10−4~7.0 × 10−4 mol·L−1, which can be quantitatively expressed by the linear Stern–Volmer equation I0/I = 8489 [Fe3+] − 0.1400, which is comparable to the previously reported better-performing materials. Competitive energy absorption and ion exchange may be responsible for the variation in fluorescence intensity of CdBPTC in different Fe3+ concentrations.

Capture, Detection, and Simultaneous Identification of Rare Circulating Tumor Cells Based on a Rhodamine 6G-Loaded Metal-Organic Framework.

Circulating tumor cells (CTCs) play a key role in the development of tumor metastasis. It will be a big step forward for CTC application as a reliable clinical liquid biopsy marker to be able to identify the captured CTCs while achieving a high capture efficiency within one analytical system. Herein, in this work, a stimuli-responsive and rhodamine 6G (Rho 6G)-entrapped fluorescent metal-organic framework (MOF) probe, named MOF-Rho 6G-DNA, was designed to capture, detect, and subsequently identify CTCs from blood samples of cancer patients. The probe was fabricated by modifying the epithelial cell adhesion molecule (EpCAM) hairpin DNA aptamer with Rho 6G enclosed and an Arm-DNA-attached UiO-66-NH2 MOF by sequence complementation. CTCs could be captured by the EpCAM hairpin DNA on the probe; as a result, Rho 6G loaded in the probe was released, and the number of CTCs was positively related to the concentration of released Rho 6G. An excellent correlation of fluorescence intensities with CTC numbers was obtained from 2 to 500 cells/mL. More importantly, the MOF-Rho 6G-DNA probe simultaneously realized rapid identification of the captured cells within 30 min by only relying on the residue Rho 6G in the MOF cavity. The captured target cells can be conveniently released from the probe using the complementary DNA sequence. These performance features of the probe were further verified by blood samples from patients of various types of tumor.

Chiral Fluorescent Metal-Organic Framework with a Pentanuclear Copper Cluster as an Efficient Luminescent Probe for Dy3+ Ion and Cyano Compounds.

Luminescent probes have been used for the detection of various heavy metals and toxic compounds. A novel sensor with excellent sensitivity and selectivity is in high demand. Herein, we designed and synthesized a three-dimensional copper-organic framework of "pcu" α-Po primitive cubic topology with a Schläfli symbol of {4.4.4.4.4.4.4.4.4.4.4.4.*.*.*}. By taking advantage of metal clusters and a triazole ligand as the metal-organic framework (MOF) components, the newly obtained MOF is stable in various environments and can be potentially used as the sensor. Remarkably, this MOF-based sensor shows high sensitivity and selectivity toward a dysprosium ion (Dy3+) in a multiple-lanthanide mixed solution. Besides, it exhibits luminescent quenching toward various cyano compounds. This chiral cluster-based network provides a potential luminescent probe for various inorganic and organic compounds with high sensitivity and selectivity.

A novel fluorescent metal-organic framework based on porphyrin and AIE for ultra-high sensitivity and selectivity detection of Pb2+ ions in aqueous solution

Lead (Pb2+) ion, as a heavy metal pollutant in water, is harmful to human health, and may cause various diseases. Therefore, it is of great necessity for people to find a new and quick way to detect Pb2+ ions in water. Based on this condition, a zinc porphyrin-based metal-organic framework (ZnTCPP-MOF) with aggregation-induced emission (AIE) was successfully synthesized via coordination polymerization of zinc (Zn2+) ions, H2TCPP and TPE-2COOH, and shows a great fluorescent phenomenon which can be used as a fluorescent probe to detect metal ions in aqueous solution. So, the ZnTCPP-MOF with a highly selective and sensitive property can be employed to detect Pb2+ ions. The experiment results exhibit that the color change of ZnTCPP-MOF could be directly discovered from bright red to colorless after adding Pb2+ ions by naked eyes. Meanwhile, the quenching curves, at lower concentrations of Pb2+ ions, exhibited an excellent linear relationship between fluorescence quenching efficiency and concentrations. The highlight of this study is that the ZnTCPP-MOF could explore Pb2+ ions in water, at very low concentrations, as well as the limit of detection (LOD) is 4.99 × 10−8 M which is an excellent data, compared with other MOFs.

Fluorescent MOF-based nanozymes for discrimination of phenylenediamine isomers and ratiometric sensing of o-phenylenediamine

Although peroxidase-like nanozymes have made great progress in bioanalysis, few current nanozyme-based biosensors are constructed for discriminating isomers of organic compounds. Herein, fluorescent metal-organic framework (MOF)-based nanozyme is utilized for phenylenediamine isomers discrimination and detection. NH2-MIL-101(Fe), as a member of Fe-based MOFs, functions as not only fluorescent indicator but also peroxidase mimics. In the presence of H2O2, NH2-MIL-101(Fe) can catalyze the oxidation of o-phenylenediamine (OPD) and p-phenylenediamine (PPD) into their corresponding oxidation products (OPDox and PPDox), which in turn quench its intrinsic fluorescence at 445 nm via inner filter effect (IFE). Differently, a new fluorescence peak at 574 nm is observed for OPDox. Thus, a ratiometric fluorescence method for the detection of OPD can be designed with the fluorescence intensity ratio F574/F445 as readout. This proposed strategy displays excellent discrimination ability for three phenylenediamines and may open new applications of MOFs in environmental science.

Chemically Stable Metal-Organic Frameworks: Rational Construction and Application Expansion.

Metal-organic frameworks (MOFs) have been attracting tremendous attention owing to their great structural diversity and functional tunability. Despite numerous inherent merits and big progress in the fundamental research (synthesizing new compounds, discovering new structures, testing associated properties, etc.), poor chemical stability of most MOFs severely hinders their involvement in practical applications, which is the final goal for developing new materials. Therefore, constructing new stable MOFs or stabilizing extant labile MOFs is quite important. As with them, some "potential" applications would come true and a lot of new applications under harsh conditions can be explored. Efficient strategies are being pursued to solve the stability problem of MOFs and thereby achieve and expand their applications.In this Account, we summarize the research advance in the design and synthesis of chemically stable MOFs, particularly those stable in acidic, basic, and aqueous systems, as well as in the exploration of their applications in several expanding fields of environment, energy, and food safety, which have been dedicated in our lab over the past decade. The strategies for accessing stable MOFs can be classified into: (a) assembling high-valent metals (hard acid, such as Zr4+, Al3+) with carboxylate ligands (hard base) for acid-stable MOFs; (b) combining low-valent metals (soft acid, such as Co2+, Ni2+) and azolate ligands (soft base, such as pyrazolate) for alkali-resistant MOFs; (c) enhancing the connectivity of the building unit; (d) contracting or rigidifying the ligand; (e) increasing the hydrophobicity of the framework; and (f) substituting liable building units with stable ones (such as metal metathesis) to obtain robust MOFs. In addition, other factors, including the geometry and symmetry of building units, framework-framework interaction, and so forth, have also been taken into account in the design and synthesis of stable MOFs. On the basis of these approaches, the stability of resulting MOFs under corresponding conditions has been remarkably enhanced.With high chemical stability achieved, the MOFs have found many new and significant applications, aiming at addressing global challenges related to environmental pollution, energy shortage, and food safety.A series of stable MOFs have been constructed for detecting and eliminating contaminations. Various fluorescent MOFs were rationally customized to be powerful platforms for sensing hazardous targets in food and water, such as dioxins, antibiotics, veterinary drugs, and heavy metal ions. Some hydrophobic MOFs even showed effective and specific capture of low-concentration volatile organic compounds.Novel MOFs with record-breaking acid/base/nucleophilic regent resistance have expanded their application scope under harsh conditions. BUT-8(Cr)A, as the most acid-stable MOF yet, showed reserved structural integrity in concentrated H2SO4 and recorded high proton conductivity; the most alkali-resistant MOF, PCN-601, retained crystallinity even in boiling saturated NaOH aqueous solution, and such base-stable MOFs composed of non-noble metal clusters and poly pyrazolate ligands also demonstrated great potential in heterogeneous catalysis in alkaline/nucleophilic systems for the first time.It is believed that this Account will provide valuable references on stable MOFs' construction as well as application expansion toward harsh conditions, thereby being helpful to promote MOF materials to step from fundamental research to practical applications.

A thermal and pH stable fluorescent metal-organic framework sensor for high selectively and sensitively sensing nitro aromatic compounds in aqueous media

A new mixed ligand coordination polymer [Cd(L)•H2O]n (1) has been synthesized under solvothermal reactions of Cd(NO3)2•4H2O and 2-amino-4, 4′-biphthalic acid (H2L). In complex 1, the coordination waters linked adjacent Cd(II) ions to form 1D chain [Cd(H2O)]n, then the -NH2 and carboxylic acid groups in the ligand connect these 1D chains into 3D framework, it shows good thermal and acid/base stability. Photoluminescent experiments show that complex 1 exhibits excellent sensitive and selective detections abilities for 2, 4, 6-trinitrophenol (TNP) and 4-nitrophenol (4-NP). As well, the detection mechanism of complex 1 for nitro compounds were also further investigated by both experimentations and calculations.

Positive Cooperative Protonation of a Metal-Organic Framework: pH-Responsive Fluorescence and Proton Conduction.

Positive cooperative binding, a phenomenon prevalent in biological processes, holds great appeal for the design of highly sensitive responsive molecules and materials. It has been demonstrated that metal-organic frameworks (MOFs) can show positive cooperative adsorption to the benefit of gas separation, but potential binding cooperativity is largely ignored in the study of sensory MOFs. Here, we report the first demonstration of positive cooperative protonation of a MOF and the relevant pH response in fluorescence and proton conduction. The MOF is built of Zr-O clusters and bipyridyl-based tetracarboxylate linkers and has excellent hydrolytic stability. It shows a unique pH response that features two synchronous abrupt turn-off and turn-on fluorescent transitions. The abrupt transitions, which afford high sensitivity to small pH fluctuations, are due to cooperative protonation of the pyridyl sites with a Hill coefficient of 1.6. The synchronous dual-emission response, which leads to visual color change, is ascribable to proton-triggered switching between (n, π*) and (π, π*) emissions. The latter emission can be quenched by electron donating anion-dependent through photoinduced electron transfer and ground-state charge transfer. Associated with cooperative protonation, the proton conductivity of the MOF is abruptly enhanced at low pH by two orders, but overhigh acid concentration is adverse because excessive anions can interrupt the conducting networks. Our work shows new perspectives of binding cooperativity in MOFs and should shed new light on the development of responsive fluorescent MOFs and proton conductive materials.

Lanthanide Metal-Organic Framework-Based Fluorescent Sensor Arrays to Discriminate and Quantify Ingredients of Natural Medicine.

The discrimination and quantification of the ingredients from natural medicines are a challenging issue due to their complicated and various structures. Metal-organic frameworks (MOFs) have shown great promise in sensing applications. Here, we report a fluorescent sensor array for rapid identification of some natural compounds using a sensor array composed of four kinds of lanthanide (Eu3+ and Tb3+) fluorescent MOFs (Ln-MOFs), which have diversified fluorescent responses to 26 active/toxic compounds including 12 saponins, 7 flavonoids, 3 stilbenes, and 4 anthraquinones. The fluorescence of the Ln-MOFs after reaction with the compounds was summarized as datasets and processed by principle component analysis (PCA) and hierarchical cluster analysis (HCA) methods. The corresponding responses of the 4 types of compounds are well separated on 2D/3D PCA score plots and HCA dendrograms. We have also tested typical blind samples by concentration-dependent PCA, and an accuracy of 100% was obtained. In addition, the response mechanisms of the Ln-MOFs to the compounds were also studied. Compared with traditional methods using liquid chromatography-mass spectrometry, the developed fluorescent sensor array provides a more efficient and economic strategy to discriminate various active/toxic ingredients in natural medicine.

Highly Stable Acid-Induced Emission-Enhancing Cd-MOFs: Synthesis, Characterization, and Detection of Glutamic Acid in Water and Fe Ions in Acid

Two novel 3D fluorescent metal-organic frameworks (MOFs), [Cd(L)(bbibp)]n (1) and [Cd(L)(bbibp)0.5]n (2), where H2L = 4,4'-(4,4'-bipyridine-2,6-diyl)dibenzoic acid and bbibp = 4,4'-bis(benzoimidaz-1-yl)biphenyl, were acquired through a conventional method and characterized via IR spectra, single-crystal X-ray diffraction, elemental analysis, thermogravimetric analysis, powder X-ray diffraction (PXRD), scanning electron microscopy, N2 adsorption-desorption isotherms, and X-ray photoelectron spectroscopy (XPS). The crystal framework of Cd-MOF 1 remained stable in the range of pH = 1.0-12.0. Interestingly, the emission peak of 1 showed a red shift and exhibited a fluorescence turn-on effect in an acidic environment. X-ray diffraction measurement revealed that the crystal structure of 1 remained unchanged after immersion in a pH = 1.0 solution. In addition, Cd-MOFs 1 and 2 displayed fluorescent quenching to l-glutamic acid with high sensitivity and selectivity. Meanwhile, 1 showed high selectivity in recognizing Fe3+ under acidic conditions, which made 1 capable of detecting Fe3+ in acidic industrial wastewater. Finally, the fluorescent sensing mechanism was carefully studied by PXRD, transient fluorescence lifetime, XPS, and UV spectroscopy.

A Fluorescent Metal-Organic Framework for Food Real-Time Visual Monitoring.

Due to increasing food-safety issues, exploiting efficient approaches for food quality assessment and instrumentation has attracted concerns worldwide. Herein, a smart evaluation system based on a fluorescent metal-organic framework (MOF) is developed for real-time visual monitoring of food freshness. Via post-synthetic modification, a ratiometric fluorescent MOF probe is constructed by covalently coupling fluorescein 5-isothiocyanate (5-FITC) with NH2 -rich lanthanide MOF. The probes exhibit a dual-emissive-responsive to biogenic amine, resulting in an increase in FITC emission along with a decrease in Eu3+ emission accompanied by a clear distinguishable color transition from orange red to green. After doping the probes on a flexible substrate, the obtained MOF composite film can be integrated with a smartphone-based portable platform easily. It is proved that this smart evaluation system can be used for on-site inspection of the freshness of raw fish samples. This work develops a fluorescent MOF-based smart evaluation system as a novel platform for application in food monitoring, which not only has enormous economic value but also holds great public health significance.

Composite fast scintillators based on high-Z fluorescent metal–organic framework nanocrystals

Scintillators, materials that produce light pulses upon interaction with ionizing radiation, are widely employed in radiation detectors. In advanced medical-imaging technologies, fast scintillators enabling a time resolution of tens of picoseconds are required to achieve high-resolution imaging at the millimetre length scale. Here we demonstrate that composite materials based on fluorescent metal–organic framework (MOF) nanocrystals can work as fast scintillators. We present a prototype scintillator fabricated by embedding MOF nanocrystals in a polymer. The MOF comprises zirconium oxo-hydroxy clusters, high-Z linking nodes interacting with the ionizing radiation, arranged in an orderly fashion at a nanometric distance from 9,10-diphenylanthracene ligand emitters. Their incorporation in the framework enables fast sensitization of the ligand fluorescence, thus avoiding issues typically arising from the intimate mixing of complementary elements. This proof-of-concept prototype device shows an ultrafast scintillation rise time of ~50 ps, thus supporting the development of new scintillators based on engineered fluorescent MOF nanocrystals. Composites of fluorescent metal–organic framework nanocrystals in a polymer are exploited to create fast scintillators with a rise time of about 50 ps.

Molecularly imprinted polymers based on magnetically fluorescent metal–organic frameworks for the selective detection of hepatitis A virus

A novel fluorescent molecularly imprinted polymers (MIPs) sensor based on a magnetic metal–organic framework (MOF) was prepared and applied in the detection of the weakly fluorescent hepatitis A virus (HAV). Luminescent MOFs can be used as both output signals and carriers; thus, a luminescent MOF (MIL-101-NH2) was used to replace conventional quantum dots and organic dyes for generating the fluorescence output signal. The constructed MIP sensor demonstrated excellent selectivity for the template recognition entities as well as a high detection sensitivity. Over the concentration range of 0.02–2.5 nM, the sensor had a detection limit of 3 pM, and a correlation coefficient of 0.997 was calculated by plotting the concentrations of the template virus against their respective fluorescence intensities. In addition, viruses were detected within 15 min by the prepared nanoparticles and remained highly selective in binary competition assays in the presence of HAV. As a result, the method developed in this work was simple, rapid, and could be used to detect HAV qualitatively and quantitatively. It has the potential to be used as a biosensor for the detection of weakly fluorescent or non-fluorescent viruses.