A newly developed dual-functional fluorescence sensing probe (phenylhydrazinyl pyridine) Schiff base (SB) has been designed with good selectivity for distinguishing Mg2+ and Al3+ metal ions in different solvent solutions. SB exhibits quick and visual turn-on fluorescence enhancement in response to Mg2+ and Al3+ detection. The addition of Mg2+ in ACN-HEPES buffer (1 : 1, v/v, pH 7.2) at (λmax=390 nm) and Al3+ in MeOH-HEPES buffer (1 : 1, v/v, pH 7.2) at (λmax=360 nm) resulted in significant enhancement of fluorescence, up to 7-9 times. These low detection limits of 7.1×10-6 M (7.1 μM) and 5.15×10-7 M (0.51 μM) for Mg2+ and Al3+, respectively, have been achieved by this solvent-controlled platform. Due to the sensing potential towards Mg2+, the probe was utilized as an imaging material for breast cancer cells. 1H-NMR studies were utilized to explore SB's sensing mechanism through turn-on fluorescence. Density functional theory (DFT) calculations were utilized to validate optimized SB and its intricate geometries, which govern the sensing mechanism in the solvent environment. Such a probe has extensive potential applications in bioimaging and the assessment of the quality of wastewater.
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