Emanuel Carrilho Carlos D. Garcia It is a great pleasure for us to present this Special Issue of ELECTROPHORESIS on Instrumentation for Capillary and Microchip Electrophoresis, the second in the series started in 2010 [2010, Vol. 31, Issue 15]. First and foremost, we would like to thank all the authors that submitted their work to this issue. Second, we would like to express our appreciation to all our colleagues that participated as reviewers, providing invaluable feedback to the authors and allowing the selection of this group of high quality papers. Last but not least, we would like to thank Dr. El Rassi and the editorial team of the journal for their efforts and constant feedback. In this issue we present three reviews, 19 research papers, and two short communications. The scope of these manuscripts is almost equally divided between instrumentation for CE and microfluidic platforms. The reviews are focused on different aspects of instrumentation for chemical analysis. The first one presents an overview of recent publications on a particular microfluidic approach that is intended to produce micro and nanoparticles. The second review included in this issue critically evaluates different designs to perform hydrodynamic injection in microchips. The third review describes instrumentation developed to perform in-situ analysis of markers of extraterrestrial life. Specifically in line with the topic of instrumentation, the issue includes a paper describing the development of a robotic platform to perform remote analysis and a paper describing instrumental aspects linked to the low-cost fabrication of microchips. Following a traditional analytical process, the issue includes five papers describing different aspects of fluidic handling and sample injection, three papers focused on separation, and five papers describing detection alternatives. Finally, the issue includes 5 additional contributions describing various applications of capillary electrophoresis. Once again, we would like to emphasize the contribution of all authors that submitted their work and made this issue a reality and we hope to continue highlighting the importance that instrumentation has for the further development of CE and microchip-CE devices. Have a pleasant read! Emanuel Carrilho Carlos D. Garcia