Fluid Manipulation Research Articles

Advances in Microchip Liquid Chromatography.

Miniaturized analytical platforms have shown significant benefits over conventional scaled systems including reduced sample requirement, reagent consumption and increased analysis speed. Microchip liquid chromatography (chipLC) is seen as the future of liquid chromatography, where novel fabrication designs and strategies, enhanced chromatographic and substrate materials, miniaturized pumping systems, and detection methodologies are all combined. In this way fluid manipulation and detection may be “simplified” to improve ease of operation, increase sensitivity as well as reduce the environmental, and physical footprints towards both modular, portable chipLC instrumentation. This review examines the background and developments in the chipLC area with emphasis on reports that have appeared in the last two years.

An Open-Source, Programmable Pneumatic Setup for Operation and Automated Control of Single- and Multi-Layer Microfluidic Devices.

Microfluidic technologies have been used across diverse disciplines (e.g. high-throughput biological measurement, fluid physics, laboratory fluid manipulation) but widespread adoption has been limited in part due to the lack of openly disseminated resources that enable non-specialist labs to make and operate their own devices. Here, we report the open-source build of a pneumatic setup capable of operating both single and multilayer (Quake-style) microfluidic devices with programmable scripting automation. This setup can operate both simple and complex devices with 48 device valve control inputs and 18 sample inputs, with modular design for easy expansion, at a fraction of the cost of similar commercial solutions. We present a detailed step-by-step guide to building the pneumatic instrumentation, as well as instructions for custom device operation using our software, Geppetto, through an easy-to-use GUI for live on-chip valve actuation and a scripting system for experiment automation. We show robust valve actuation with near real-time software feedback and demonstrate use of the setup for high-throughput biochemical measurements on-chip. This open-source setup will enable specialists and novices alike to run microfluidic devices easily in their own laboratories.

Semi-Empirical Estimation of Dean Flow Velocity in Curved Microchannels

Curved and spiral microfluidic channels are widely used in particle and cell sorting applications. However, the average Dean velocity of secondary vortices which is an important design parameter in these devices cannot be estimated precisely with the current knowledge in the field. In this paper, we used co-flows of dyed liquids in curved microchannels with different radii of curvatures and monitored the lateral displacement of fluids using optical microscopy. A quantitative Switching Index parameter was then introduced to calculate the average Dean velocity in these channels. Additionally, we developed a validated numerical model to expand our investigations to elucidating the effects of channel hydraulic diameter, width, and height as well as fluid kinematic viscosity on Dean velocity. Accordingly, a non-dimensional comprehensive correlation was developed based on our numerical model and validated against experimental results. The proposed correlation can be used extensively for the design of curved microchannels for manipulation of fluids, particles, and biological substances in spiral microfluidic devices.

Integrated obstacle microstructures for gas-liquid separation and flow switching in microfluidic networks

Split-flow is a fundamental manipulation method for liquid transport, which is challenging in microfluidics by simple structures, owing to the distinct flow mechanism in microchannels. Herein, we present a new strategy for fabricating switch-alternative fluid-splitters by reasonably embedding obstacle microstructures in microchannels. Precise confinement of triple-phase (gas, fluid front, obstacle microstructure) contact line of fluid fronts on the microstructures provides the device with advantage in controlling the flow motion of fluid in microchannels. The microstructures could act as a burst microvalve, and duration of fluid front flowing through the microvalve is essentially controlled by the quantity of the obstacles. Based on appropriate arrangement of the obstacles, we demonstrate that fluid-splitters with four and eight flow states of water and blood in microchannels could be obtained. In particular, implementation of the fluid splitters mostly relies on the regulation of the triple-phase contact line, which simplifies the complex equipments and enhances the compatibility of the devices. It is believed that the fabrication strategy of the switch-alternative fluid-splitters can be extensively used for disposable manipulation of fluid in microfluidic networks.

Do Bodybuilders Use Evidence-Based Nutrition Strategies to Manipulate Physique?

Competitive bodybuilders undergo strict dietary and training practices to achieve an extremely lean and muscular physique. The purpose of this study was to identify and describe different dietary strategies used by bodybuilders, their rationale, and the sources of information from which these strategies are gathered. In-depth interviews were conducted with seven experienced (10.4 ± 3.4 years bodybuilding experience), male, natural bodybuilders. Participants were asked about training, dietary and supplement practices, and information resources for bodybuilding strategies. Interviews were transcribed verbatim and analyzed using qualitative content analysis. During the off-season, energy intake was higher and less restricted than during the in-season to aid in muscle hypertrophy. There was a focus on high protein intake with adequate carbohydrate to permit high training loads. To create an energy deficit and loss of fat mass, energy intake was gradually and progressively reduced during the in-season via a reduction in carbohydrate and fat intake. The rationale for weekly higher carbohydrate refeed days was to offset declines in metabolic rate and fatigue, while in the final “peak week” before competition, the reasoning for fluid and sodium manipulation and carbohydrate loading was to enhance the appearance of leanness and vascularity. Other bodybuilders, coaches and the internet were significant sources of information. Despite the common perception of extreme, non-evidence-based regimens, these bodybuilders reported predominantly using strategies which are recognized as evidence-based, developed over many years of experience. Additionally, novel strategies such as weekly refeed days to enhance fat loss, and sodium and fluid manipulation, warrant further investigation to evaluate their efficacy and safety.

Investigations of offshore breaking wave impacts on a large offshore structure

This paper describes numerical and laboratory investigations that have been carried out to gain a better understanding of the physical processes involved in offshore breaking wave impacts on a large offshore structure. The findings are relevant to offshore and coastal structures and to identifying the extreme loads, peak pressures and maximum run-up needed for their design. A truncated wall in a wave flume is used to represent a vertical section of an FPSO (Floating Production Storage and Offloading) hull, which is a typical large offshore structure. Four types of wave impact were identified in the tests, and are referred to as slightly-breaking, flip-through, large air pocket and broken wave impacts. Physical modelling was undertaken in Plymouth University’s COAST Laboratory and the open source Computational Fluid Dynamics (CFD) package-Open Field Operation and Manipulation (OpenFOAM) was adopted to study focused wave generation and wave impact on the hull. The method solves incompressible Unsteady Reynolds-averaged Navier–Stokes Equations (URANSE) using a finite volume method with two phase flows. A Volume of Fluid (VoF) interface capturing approach is used to model the free surface. A NewWave boundary condition is used to generate focused wave groups based on the first plus second-order (hereafter second-order) Stokes wave theory in the Numerical Wave Tank (NWT). By changing the focus location with respect to the wall, the wave impact type was altered in both the numerical and laboratory investigations.The results show that for the four wave impact types tested good agreement was achieved between numerical predictions and experimental measurements of surface elevation, run up and impact force. The peak pressures predicted by the simulation are lower than the experimentally measured results due to time step constraints, although the shape of the pressure time history is very similar. Four distinct wave impact types are identified for the vertical hull section and are found to be similar in character to those observed for a full depth vertical wall. The predicted force on the hull is found to be greatest for the large air pocket impact, and the highest run-up for the slightly-breaking wave impact. The pressure records show a high degree of spatial and temporal variation though the highest pressure recorded at any location was due to flip-through. This research has shown that different characteristic wave impact types are responsible for maximum load and greatest wave run-up and so need to be considered separately for design purposes.

Multiplexed capillary microfluidic immunoassay with smartphone data acquisition for parallel mycotoxin detection

The field of microfluidics holds great promise for the development of simple and portable lab-on-a-chip systems. The use of capillarity as a means of fluidic manipulation in lab-on-a-chip systems can potentially reduce the complexity of the instrumentation and allow the development of user-friendly devices for point-of-need analyses. In this work, a PDMS microchannel-based, colorimetric, autonomous capillary chip provides a multiplexed and semi-quantitative immunodetection assay. Results are acquired using a standard smartphone camera and analyzed with a simple gray scale quantification procedure. The performance of this device was tested for the simultaneous detection of the mycotoxins ochratoxin A (OTA), aflatoxin B1 (AFB1) and deoxynivalenol (DON) which are strictly regulated food contaminants with severe detrimental effects on human and animal health. The multiplexed assay was performed approximately within 10min and the achieved sensitivities of<40, 0.1–0.2 and<10ng/mL for OTA, AFB1 and DON, respectively, fall within the majority of currently enforced regulatory and/or recommended limits. Furthermore, to assess the potential of the device to analyze real samples, the immunoassay was successfully validated for these 3 mycotoxins in a corn-based feed sample after a simple sample preparation procedure.

A model for controlled dosing of femto-litre volume liquids using hollow microcantilever

A hollow microcantilever is used instead of a conventional microcantilever to dispense and aspirate liquids in the femto-litre (10-15 L) volume range in an atomic force microscope (AFM) setup. The inherent force sensing capability of the cantilever is used to monitor the fluid manipulation in-situ. At this small scale, parameters like: surface energy, evaporation, viscosity and temperature become important for controlled manipulation. In a conventional AFM, these parameters are usually not taken into consideration as feedback parameters. In the present work, we report initial experimental results on the dosing process and an analytical dynamic model of the process. The model is based on the liquid bridge between the cantilever tip and the substrate, which can describe the dosing process with variance accounted for (VAF) larger than 90%. We aim to use this model and implement a control system for precise dispensing and aspiration.

Editorial for Special Issue: Advances in Microfluidic Devices for Cell Handling and Analysis

Microfluidics is a technology that is expanding rapidly in many areas of research, especially in the biological areas of cell handling and analysis.[...]

A Perspective on the Rise of Optofluidics and the Future

In the recent past, the field of optofluidics has thrived from the immense efforts of researchers from diverse communities. The concept of optofluidics combines optics and microfluidics to exploit novel properties and functionalities. In the very beginning, the unique properties of liquid, such as mobility, fungibility and deformability, initiated the motivation to develop optical elements or functions using fluid interfaces. Later on, the advancements of microelectromechanical system (MEMS) and microfluidic technologies enabled the realization of optofluidic components through the precise manipulation of fluids at microscale thus making it possible to streamline complex fabrication processes. The optofluidic system aims to fully integrate optical functions on a single chip instead of using external bulky optics, which can consequently lower the cost of system, downsize the system and make it promising for point-of-care diagnosis. This perspective gives an overview of the recent developments in the field of optofluidics. Firstly, the fundamental optofluidic components will be discussed and are categorized according to their basic working mechanisms, followed by the discussions on the functional instrumentations of the optofluidic components, as well as the current commercialization aspects of optofluidics. The paper concludes with the critical challenges that might hamper the transformation of optofluidic technologies from lab-based procedures to practical usages and commercialization.

Droplets on Microdecorated Surfaces: Evolution of the Polygonal Contact Line

Interaction of liquids with surfaces is ubiquitous in our physical environment as well as many engineering applications. Recent advances on this topic have not only provided us with valuable insight into nature's design, but also enabled improved fluidic manipulation for liquid-based printing applications such as biomicroarrays for protein and DNA sequencing, multicolor polymer-based LED displays, inkjet printing, and solder droplet printing, among others. For example, droplet contact lines, which are typically circular on a smooth and homogeneous surface, when deposited on a microdecorated surface may take various common polygonal shapes such as squares, rectangles, hexagons, octagons and dodecagons. These polygonal contact line shapes are highly stable due to the local energy barriers associated with the anisotropy in depinning contact angles. In addition to the knowledge of the eventual contact line shapes, liquid-based printing applications would require accurate prediction of temporal evolution of contact line on these surfaces. In this work, we model and validate the evolution of droplets on microdecorated surfaces with microgoniometry experiments reported in the literature. We show that various metastable contact line shapes are formed in-between the well-known stable polygonal contact line shapes usually observed in experiments. We elucidate that the movement of the contact line between adjacent micropillars can primarily be categorized as primary zipping and transition zipping. Primary zipping occurs when the contact line moves radially outward to the next row of pillars, often resulting in the formation of a metastable contact line shape. Conversely, metastable droplet attains stable polygonal contact line shape via transition zipping wherein the contact line advances sideways. We believe that the current simulation approach can be effectively utilized for designing optimized textured surfaces for applications where control over liquid supply via surface design is required.

Microfluidics:A Boon for Biological Research

Microfluidics is an emerging new interdisciplinary field that deals with the manipulation of fluids at the microscale and nanoscale. Having its origins in other areas of science and technology, microfluidics is slowly beginning to make radical changes in various fields of biological sciences. The exclusivity of fluid behaviour at the microscale offers a large number of advantages in biological research such as miniaturization of assays, faster sample processing and rapid detection. This article provides a concise overview of the applications of microfluidics technology in some of the major disciplines of biological research. Furthermore, it also mentions the hurdles that microfluidics is facing and the solutions that are envisaged for the future to make it a widely available, reliable and cost-effective technology.

Characterization of an induced pressure pumping force for microfluidics

The electro-osmotic pumping and pressure-driven manipulation of fluids are considered as the most common strategies in microfluidic devices. However, both of them exhibit major disadvantages such as hard integration and high reagent consumption, and they are destructive methods for detection and photo bleaching. In this paper, an electric field-effect flow control approach, combining the electro-osmotic pumping force and the pressure-driven pumping force, was developed to generate the induced pressure-driven flow in a T-shaped microfluidic chip. Electro-osmotic flow between the T-intersection and two reservoirs was demonstrated, and it provided a stable, continuous, and electric field-free flow in the section of the microchannel without the electrodes. The velocity of the induced pressure-driven flow was linearly proportional to the applied voltages. Both numerical and experimental investigations were conducted to prove the concept, and the experimental results showed good agreement with the numerical simulations. In comparison to other induced pressure pumping methods, this approach can induce a high and controllable pressure drop in the electric field-free segment, subsequently causing an induced pressure-driven flow for transporting particles or biological cells. In addition, the generation of bubbles and the blocking of the microchannel are avoided.

Vertical jetting induced by shear horizontal leaky surface acoustic wave on 36° Y-X LiTaO3

Shear horizontal surface acoustic waves (SH-SAWs) have been regarded as good candidates for liquid sensing applications but are inefficient in fluid manipulation due to a minimal fluid coupling between the fluid and acoustic waves. However, in this letter, a vertical jetting function was realized using the SH-SAW generated from a 36° Y-X LiTaO3 SAW device. The jetting of the droplet induced by the SH-SAWs was observed nearly along the vertical direction, and the aspect ratio of the liquid beam is proportional to the applied power before breaking up, which is dramatically different from those generated from the conventional Rayleigh SAWs. By conducting theoretical simulation and experimental investigation on the SH-SAWs systematically, we concluded that the wave/energy pressure dissipated into the sessile droplets causes this vertical ejection on the device surface.

Photoresponsive Passive Micromixers Based on Spiropyran Size-Tunable Hydrogels.

Microfluidic devices allow the manipulation of fluids down to the micrometer scale and are receiving a lot of attention for applications where low volumes and high throughputs are required. In these micro channels, laminar flow usually dominates, which requires long residence times of the fluids, limiting the flow speed and throughput. Here a switchable passive mixer has been developed to control mixing and to easily clean microchannels. The mixer is based on a photoresponsive spiropyran based hydrogel of which the dimensions can be tuned by changing the intensity of the light. The size-tunable gels have been used to fabricate a passive slanted groove mixer that can be switched off by light allowing to change mixing of microfluidics to non-mixed flows. These findings open new possibilities for multi-purpose microfluidic devices where mixers and valves can be tuned by light.

Materials for Microfluidic Immunoassays: A Review.

Conventional immunoassays suffer from at least one of these following limitations: long processing time, high costs, poor user-friendliness, technical complexity, poor sensitivity and specificity. Microfluidics, a technology characterized by the engineered manipulation of fluids in channels with characteristic lengthscale of tens of micrometers, has shown considerable promise for improving immunoassays that could overcome these limitations in medical diagnostics and biology research. The combination of microfluidics and immunoassay can detect biomarkers with faster assay time, reduced volumes of reagents, lower power requirements, and higher levels of integration and automation compared to traditional approaches. This review focuses on the materials-related aspects of the recent advances in microfluidics-based immunoassays for point-of-care (POC) diagnostics of biomarkers. We compare the materials for microfluidic chips fabrication in five aspects: fabrication, integration, function, modification and cost, and describe their advantages and drawbacks. In addition, we review materials for modifying antibodies to improve the performance of the reaction of immunoassay. We also review the state of the art in microfluidic immunoassays POC platforms, from the laboratory to routine clinical practice, and also commercial products in the market. Finally, we discuss the current challenges and future developments in microfluidic immunoassays.

Superamphiphobic Bionic Proboscis for Contamination-Free Manipulation of Nano and Core-Shell Droplets.

Manipulation of nanoliter droplets is a key step for many emerging technologies including ultracompact microfluidics devices, 3D and flexible electronic printing. Despite progress, contamination-free generation and release of nanoliter droplets by compact low-cost devices remains elusive. In the present study, inspired by butterflies' minute manipulation of fluids, the authors have engineered a superamphiphobic bionic proboscis (SAP) layout that surpasses synthetic and natural designs. The authors demonstrate the scalable fabrication of SAPs with tunable inner diameters down to 50 µm by the rapid gas-phase nanotexturing of the outer and inner surfaces of readily available hypodermic needles. Optimized SAPs achieve contamination-free manipulation of water and oil droplets down to a liquid surface tension of 26.56 mN m-1 and a volume of 10 nL. The unique potential of this layout is showcased by the rapid and carefully controlled in-air synthesis of core-shell droplets with well-controlled compositions. These findings provide a new low-cost tool for high-precision manipulation of nanoliter droplets, offering a powerful alternative to established thermal- and electrodynamic-based devices.

Droplet-based microfluidics detector for bioaerosol detection

Detection of bioaerosols is important in fields ranging from environmental health monitoring to biosurveillance, and current detector weaknesses have motivated the development of new technologies. In this work, a detector was built, which applies the principles of droplet microfluidics to bioaerosol detection. Droplet microfluidics is a subfield of microfluidics based on the creation of monodisperse microdroplets with compartmentalized reagents and supports enhanced assays and fluidic manipulations. The bioaerosol detector operates by aerodynamically focusing aerosols directly into these droplets to harness the benefits of the microreactor environment. A breadboard detector system, which consisted of an aerodynamic focusing lens, aerosol-focusing capillary, microfluidic droplet chip, and optical microscope, was constructed. Computational fluid dynamic simulations and Lagrangian particle tracking modeling were conducted to identify the optimal conditions for focusing. Preliminary experiments, where aerosols were deposited onto a solid substrate, demonstrated sub 200-µm spot diameters for aerodynamic diameters of 2–5 µm. Test aerosols were then generated, and collected into the microfluidic liquid interface on the chip as verified by microscopy. Recovery efficiency of the aerosols was dependent on aerosol size and ranged from about 27% to nearly 100%. Finally, to prove bioaerosol collection and detection, a droplet propidium iodide (PI) assay was performed: the system distinguished between E. coli and non-biological aerosols within 20 s. Overall, this work established the technique of direct collection of bioaerosols into a convenient droplet microfluidic platform for detection.Copyright © 2017 The Johns Hopkins University Applied Physics Laboratory

Electrowetting-actuated liquid metal for RF applications

Electrowetting is well-established as a fluid manipulation technique in such areas as lab-on-a-chip, visible light optics, and displays, yet has seen far less implementation in the field of radio-frequency (RF) electronics and electromagnetics. This is primarily due to a lack of appropriate materials selection and control in these devices. Low loss RF conductive fluids such as room temperature liquid metals (i.e. Hg, EGaIn, Galinstan) are by far the leading choice of active material due to their superior electrical properties but require high actuating voltages due to their inherently high surface tensions (>400 mN m−1) which often lead to dielectric breakdown. While the toxicity of Hg encourages the pursuit of non-toxic alternatives such as gallium alloys, the native surface oxide formation often prohibits reliable device functionality. Additionally, traditional electrowetting architectures rely on lossy electrode materials which degrade RF transmission efficiencies and result in non-reversible material diffusion at the electrode/liquid metal contact. In this work, we report on approaches to utilize liquid metals in electrowetting on dielectric (EWOD) devices that resolve all of these challenges by judicious choice of novel electrode materials, dielectric fluid, and device architecture. A functional RF device, namely an electromagnetic polarizer, is demonstrated that can be activated on demand through EWOD and provides an average signal attenuation of 12.91 dB in the on state and 1.46 dB in the off state over the range of 8–9.2 GHz, with a switching speed of about 12 ms. These results can be further extended to other RF applications such as tunable antennas, transmission lines, and switchable metasurfaces.

Thermal-Responsive Anisotropic Wetting Microstructures for Manipulation of Fluids in Microfluidics.

We show morphology-patterned stripes modified by thermal-responsive polymer for smartly guiding flow motion of fluid in chips. With a two-step modification process, we fabricated PNIPAAm-modified Si stripes on silicon slides, which were employed as substrates for fluid manipulation in microchannels. When the system temperature switches between above and below the lower critical solution temperature (LCST) of PNIPAAm, the wettability of the substrates also switches between strong anisotropy and weak anisotropy, which resulted in anisotropic (even unidirectional) flow and isotropic flow behavior of liquid in microchannels. The thermal-responsive flow motion of fluid in the chip is influenced by the applied pressure, the thickness of PNIPAAm, and dimension of the microchannels. Moreover, we measured the feasible applied pressure scopes under different structure factors. Because of the excellent reversibility and quick switching speed, the chip could be used as a thermal-responsive microvalve. Through tuning the system temperature and adding the assistant gas, we realized successive "valve" function. We believe that the practical and simple chip could be widely utilized in medical detection, immunodetection, protein analysis, and cell cultures.