In order to study the dynamic aspects of removal, conjugation and transformations of free dehydroisoandrosterone (DHA) and the transformations, particularly hydrolysis, of dehydroisoandrosterone sulfate (DHAS), DHA-7α-3H was administered to two subjects and DHAS-7α-3H was given to a single patient. Within 2 hr after the injection of DHAS-3H, free DHA contained 0.19% of the radioactivity in plasma and maintained this value during the remaining 5 hr of the study. Androsterone (A) and etiocholanolone (E) isolated from the sulfate and glucosiduronate fractions of plasma were also radioactive demonstrating that a portion of them was derived from the tracer, presumably via hydrolysis of DHAS. Within 30 min after injection of DHA-3H, 90% of the radioactivity in the plasma was present in steroid conjugates of which 30% consisted of glucosiduronates. By 7 hr, over 99% of the 3H was present in the conjugates, glucosiduronates comprising less than 10% of the fraction. At this time, circulating free DHA contained 0.7% of the plasma radioactivity. These observations of a rapid and continuing hydrolysis of DHAS as well as the persistence of free DHA in the circulation provide evidence for the presence of a baseline “level” of free DHA below which fluctuating plasma concentrations of the hormone (from episodic secretion) do not fall.