Flow Shear Stress Research Articles

Form Shear Stress Correction in Bulk Flow Analysis of Grooved Seals Based on Effective Film Thickness

Abstract Bulk flow models for grooved annular seals provide computationally efficient static and dynamic response predictions, though heavy reliance on empirical relationships often leads to undesirable levels of uncertainty. The flow complexity caused by the grooves adds difficulty to shear stress modeling for these seals. This study seeks to improve shear stress modeling for grooved seals through the identification and quantification of the additional bulk flow shear stress contributions within the groove region. Through single groove computational fluid dynamics (CFD) simulations and an effective film thickness analysis framework, the additional groove shear stress component is identified as a form shear stress (FSS) due to its clear relationship to the effective film thickness behavior. The FSS is quantified as a correction to traditional shear stress definitions. Predictive models for the FSS are developed as functions of the ratio of circumferential to axial Reynolds number and the total resultant Reynolds number. Implementation of the FSS models into a simplified bulk flow method delivers leakage predictions for three seal cases within 10% of the experimental results and qualitative agreement in predicted circumferential velocity profiles while eliminating the need for an assumed groove loss coefficient. This is the first paper to utilize an effective film thickness-based procedure to quantify and model the FSS component in grooved seal bulk flow analysis. The demonstrated predictive capability and widespread applicability of the models and approach presented in this paper provide an avenue for significant improvements in grooved seal bulk flow prediction accuracy through improved shear stress modeling.

A Mathematical Method for Estimating the Critical Slope Angle of Sheet Erosion

Estimating the critical slope angle (CSA) for sheet erosion is important for the precision estimation of sheet erosion and the development of erosion control practices. This study developed mathematical equations considering rainfall intensity and soil infiltration to efficiently estimate both instantaneous (at a given instant during rainfall) and cumulative CSAs, while also providing a valuable explanation for the change in CSA. The mathematical equations were consistent with observations from runoff plots (NSE = −1.01) of loess soils from Zhangjiakou (China) and simulation results (NSE = 0.96) from the Water Erosion Prediction Project model for a loam soil in Montana (USA). Estimated instantaneous CSA determined by the mathematical equations increased as the ratio of rainfall intensity to soil infiltration (I/f) increased, resulting in higher observed cumulative CSA after heavy versus normal rainfall events. Heavy rainfall, compacted soil, and varying rainfall duration affected the CSA by changing the I/f ratio. Maximum instantaneous CSA provided a better prediction of changes in soil erosion dynamics than that obtained from CSAs determined by field observations or experimental simulations. The mathematical equations illustrate the underlying physical mechanisms by which rainfall intensity and soil infiltration affect the CSA through changing the shear stress of overland flow. The results of this study provide critical information for guiding the development of effective soil erosion control strategies.

Field simulation experiment on the relationships between hydrodynamics and soil detachment rate in formed rills

The dynamics of rill erosion are crucial for accurate modelling of soil erosion and vary across rill development stages. Research has largely concentrated on the rill formation process, with limited insights into formed rills under field conditions. Given the importance of soil detachment rate in the intensity and dynamics of soil erosion, this study aims to investigate the correlations between the soil detachment rate and hydrodynamic parameters in formed rills through field experiments. Therefore, the objective of this study was to explore the relationships between hydrodynamic parameters and the soil detachment rate of formed rills through field scouring experiments. The experiments were conducted on formed rills with flow discharges of 6, 9, 12, 15, 18, and 21 L·min−1 in a field runoff plot (with length of 18, width of 1 m, and slope gradient of 17.5 %). The results showed that the soil detachment rate remained stable fluctuations over time under different flow discharges. The rill flow velocity varied from 0.191 to 0.301 m·s−1, and increased with the increase of flow discharge. Except for the low-flow discharge (6 and 9 L·min−1), the rill flows manifested as super-critical turbulent flow. The values of flow shear stress, stream power, unit stream power, and unit energy were within the scope of 8.776–12.517 Pa, 1.647–3.757 W·m−2, 0.033–0.052 m·s−1 and 0.007–0.013 m, respectively. The soil detachment rate was significantly negatively correlated with Darcy–Weisbach friction, whereas demonstrated a significant positive correlation with Reynolds number and stream power. The soil detachment rate of formed rills was accurately predicted by flow stream power using a linear function (R2 = 0.918). The results of this study provided insights into hydrodynamics and soil detachment rate of purple soil under natural rainfall conditions.

Effect of surface engineering methods on refrigerant evaporating flow characteristics in annular tubes: Experimental approach

Techniques such as mechanical, electrochemical, and chemical etching are recognized as functional strategies for modifying surface roughness, which in turn manipulates surface energy. This research aims to delve into the influence of these factors on the heat transfer and pressure drop characteristics of two-phase R134a refrigerant flow during boiling inside horizontal annular concentric tubes with internal heat flux. The study scrutinizes the heat transfer coefficient and frictional pressure drop for R134a evaporating flow in an annular tube featuring an inner diameter of 28.6 mm and an outer diameter of 42 mm. The exploration spans a broad range of parameters, which include vapor quality from 0.15 to 0.8, mass flux from 6.72 to 20.16 kg/m2s, internal heat flux from 0.608 to 2.432 kW/m2, and varying surface characteristics. The results show that escalating surface roughness via mechanical techniques introduces greater resistance to flow shear stress in comparison to other methods. Moreover, an increase in surface roughness contributes to a reduction in contact angle and an expansion of the heating area, thereby enhancing both heat transfer coefficient and pressure drop. However, the implementation of low-energy coating holds an insignificant influence on R134a heat transfer characteristics, potentially due to its diminutive surface tension. This results in minor variations in the refrigerant contact angle in relation to the coatings. Performance evaluation criteria have been employed to assess the optimal thermal performance of the surface engineering method, with peak performance achieved at a mass flux of 20.16 Kg/m2s and an internal heat flux of 2.432 kW/m2. Ultimately, the study corroborates the experimental procedure by comparing the observed outcomes with established empirical correlations for R134a flow boiling.

Single-Cell RNA Sequencing Reveals That Adaptation of Human Aortic Endothelial Cells to Antiproliferative Therapies Is Modulated by Flow-Induced Shear Stress.

Endothelial cells (ECs) are capable of quickly responding in a coordinated manner to a wide array of stresses to maintain vascular homeostasis. Loss of EC cellular adaptation may be a potential marker for cardiovascular disease and a predictor of poor response to endovascular pharmacological interventions such as drug-eluting stents. Here, we report single-cell transcriptional profiling of ECs exposed to multiple stimulus classes to evaluate EC adaptation. Human aortic ECs were costimulated with both pathophysiological flows mimicking shear stress levels found in the human aorta (laminar and turbulent, ranging from 2.5 to 30 dynes/cm2) and clinically relevant antiproliferative drugs, namely paclitaxel and rapamycin. EC state in response to these stimuli was defined using single-cell RNA sequencing. We identified differentially expressed genes and inferred the TF (transcription factor) landscape modulated by flow shear stress using single-cell RNA sequencing. These flow-sensitive markers differentiated previously identified spatially distinct subpopulations of ECs in the murine aorta. Moreover, distinct transcriptional modules defined flow- and drug-responsive EC adaptation singly and in combination. Flow shear stress was the dominant driver of EC state, altering their response to pharmacological therapies. We showed that flow shear stress modulates the cellular capacity of ECs to respond to paclitaxel and rapamycin administration, suggesting that while responding to different flow patterns, ECs experience an impairment in their transcriptional adaptation to other stimuli.

Modelling blood flow in coronary arteries: Newtonian or shear-thinning non-Newtonian rheology?

BackgroundThe combination of medical imaging and computational hemodynamics is a promising technology to diagnose/prognose coronary artery disease (CAD). However, the clinical translation of in silico hemodynamic models is still hampered by assumptions/idealizations that must be introduced in model-based strategies and that necessarily imply uncertainty. This study aims to provide a definite answer to the open question of how to properly model blood rheological properties in computational fluid dynamics (CFD) simulations of coronary hemodynamics. MethodsThe geometry of the right coronary artery (RCA) of 144 hemodynamically stable patients with different stenosis degree were reconstructed from angiography. On them, unsteady-state CFD simulations were carried out. On each reconstructed RCA two different simulation strategies were applied to account for blood rheological properties, implementing (i) a Newtonian (N) and (ii) a shear-thinning non-Newtonian (non-N) rheological model. Their impact was evaluated in terms of wall shear stress (WSS magnitude, multidirectionality, topological skeleton) and helical flow (strength, topology) profiles. Additionally, luminal surface areas (SAs) exposed to shear disturbances were identified and the co-localization of paired N and non-N SAs was quantified in terms of similarity index (SI). ResultsThe comparison between paired N vs. shear-thinning non-N simulations revealed remarkably similar profiles of WSS-based and helicity-based quantities, independent of the adopted blood rheology model and of the degree of stenosis of the vessel. Statistically, for each paired N and non-N hemodynamic quantity emerged negligible bias from Bland-Altman plots, and strong positive linear correlation (r > 0.94 for almost all the WSS-based quantities, r > 0.99 for helicity-based quantities). Moreover, a remarkable co-localization of N vs. non-N luminal SAs exposed to disturbed shear clearly emerged (SI distribution 0.95 [0.93, 0.97]). Helical flow topology resulted to be unaffected by blood rheological properties. ConclusionsThis study, performed on 288 angio-based CFD simulations on 144 RCA models presenting with different degrees of stenosis, suggests that the assumptions on blood rheology have negligible impact both on WSS and helical flow profiles associated with CAD, thus definitively answering to the question “is Newtonian assumption for blood rheology adequate in coronary hemodynamics simulations?”.

Fluid flow shear stress and tissue remodeling-an orthodontic perspective: evidence synthesis and differential gene expression network analysis.

Introduction: This study aimed to identify and analyze in vitro studies investigating the biological effect of fluid-flow shear stress (FSS) on cells found in the periodontal ligament and bone tissue. Method: We followed the PRISMA guideline for systematic reviews. A PubMed search strategy was developed, studies were selected according to predefined eligibility criteria, and the risk of bias was assessed. Relevant data related to cell source, applied FSS, and locus-specific expression were extracted. Based on this evidence synthesis and, as an original part of this work, analysis of differential gene expression using over-representation and network-analysis was performed. Five relevant publicly available gene expression datasets were analyzed using gene set enrichment analysis (GSEA). Result: A total of 6,974 articles were identified. Titles and abstracts were screened, and 218 articles were selected for full-text assessment. Finally, 120 articles were included in this study. Sample size determination and statistical analysis related to methodological quality and the ethical statement item in reporting quality were most frequently identified as high risk of bias. The analyzed studies mostly used custom-made fluid-flow apparatuses (61.7%). FSS was most frequently applied for 0.5h, 1h, or 2h, whereas FSS magnitudes ranged from 6 to 20dyn/cm2 depending on cell type and flow profile. Fluid-flow frequencies of 1Hz in human cells and 1 and 5Hz in mouse cells were mostly applied. FSS upregulated genes/metabolites responsible for tissue formation (AKT1, alkaline phosphatase, BGLAP, BMP2, Ca2+, COL1A1, CTNNB1, GJA1, MAPK1/MAPK3, PDPN, RUNX2, SPP1, TNFRSF11B, VEGFA, WNT3A) and inflammation (nitric oxide, PGE-2, PGI-2, PTGS1, PTGS2). Protein-protein interaction networks were constructed and analyzed using over-representation analysis and GSEA to identify shared signaling pathways. Conclusion: To our knowledge, this is the first review giving a comprehensive overview and discussion of methodological technical details regarding fluid flow application in 2D cell culture in vitro experimental conditions. Therefore, it is not only providing valuable information about cellular molecular events and their quantitative and qualitative analysis, but also confirming the reproducibility of previously published results.

An approach for computational blood flow under the joint effects of the electro-osmotic, magnetic field, chemical reaction and porosity

PurposeThe purpose of this study is to analyze the two-dimensional blood flow in the artery slant from the axis at an angle with mild stenosis under the joint effects of the electro-osmotic, magnetic field, chemical reaction and porosity using a new analytical method. In addition, the mathematical model presented by the researchers Tripathi and Sharma (2018c) was successfully developed by adding the effect of electro-osmosis and studying the impact of the new addition in the developed model on blood flow.Design/methodology/approachA new analytical method was used to find the analytical approximate solutions of two-dimensional blood flow in artery slant from the axis at an angle with mild stenosis. This technique is based on integrating the Akbari-Ganji and the homotopy perturbation methods.FindingsThe results of axial velocity, concentration, temperature and the wall shear stress for blood flow were analyzed in the cases of the absence and presence of electro-osmosis. Furthermore, in these two states of electro-osmosis, a contour plot was created to show the difference in the profile of velocity to the flow of blood when the magnetic field was increased and the altitude of stenosis was increased. The results showed that the new technique is effective and has high accuracy to determine the analytical approximate solutions of two-dimensional blood flow in artery slant from the axis at an angle with mild stenosis. The validity, utility and necessity of the new method were illustrated from the graphs of the new solutions; in addition, there is an excellent agreement with the results of previous studies.Originality/valueThis paper focuses on developing the mathematical model which was presented by the researchers Tripathi and Sharma (2018c), by adding the effect of the electro-osmosis to it, which has been successfully developed. According to the authors’ modest information, the new system has not been studied before. This current problem is solved by using an innovative approach known as the Akbari-Ganji homotopy perturbation method (AGHPM) which has not been used before in two cases: the presence and absence of the effect of electro-osmosis. This new technique afford new with effective and has high accuracy results. Furthermore, the new study (i.e. adding effect of electro-osmosis) with the applications of (variable viscosity, magnetic field, chemical reaction and porosity) illustrated the importance of applying electro-osmosis and how doctors can benefit from it during surgeries through proper use.

Enhanced external counterpulsation treatment regulates blood flow and wall shear stress metrics in femoral artery: An in vivo study in healthy subjects

As a non-invasive assisted circulation therapy, enhanced external counterpulsation (EECP) has demonstrated potential in treatment of lower-extremity arterial disease (LEAD). However, the underlying hemodynamic mechanism remains unclear. This study aimed to conduct the first prospective investigation of the EECP-induced responses of blood flow behavior and wall shear stress (WSS) metrics in the femoral artery. Twelve healthy male volunteers were enrolled. A Doppler ultrasound-basedapproach was introduced for the in vivo determination of blood flow in the common femoral artery (CFA) and superficial femoral artery (SFA) during EECP intervention, with incremental treatment pressures ranging from 10 to 40 kPa. Three-dimensional subject-specific numerical models were developed in 6 subjects to quantitatively assess variations in WSS-derived hemodynamic metrics in the femoral bifurcation. A mesh-independence analysis was performed. Our results indicated that, compared to the pre-EECP condition, both the antegrade and retrograde blood flow volumes in the CFA and SFA were significantly augmented during EECP intervention, while the heart rate remained constant. The time average shear stress (TAWSS) over the entire femoral bifurcation increased by 32.41%, 121.30%, 178.24%, and 214.81% during EECP with treatment pressures of 10 kPa, 20 kPa, 30 kPa, and 40 kPa, respectively. The mean relative resident time (RRT) decreased by 24.53%, 61.01%, 69.81%, and 77.99%, respectively. The percentage of area with low TAWSS in the femoral artery dropped to nearly zero during EECP with a treatment pressure greater than or equal to 30 kPa. We suggest that EECP is an effective and non-invasive approach for regulating blood flow and WSS in lower extremity arteries.

Hall current and thermal radiation effects of 3D rotating hybrid nanofluid reactive flow via stretched plate with internal heat absorption

The present analysis deals with the impact of a magnetic field, joule heating, rotation parameter, and Hall current, as well as nonlinear thermal radiation, on a rotating hybrid Fe3O4/Al2O3 nanofluid over-stretched plate in the presence of a chemical reaction with thermophoresis and a Brownian motion parameter. The primary focus of this research is on the Brownian motion parameter. Similar transformations are used to translate the governing partial differential equations into a set of nonlinear ordinary differential equations. The shooting technique obtains numerical solutions for that system of equations. The impact of various entry parameters on transversal and longitudinal velocities, temperature, heat flow and surface shear stress are studied numerically and graphically. It was shown that there is a strong connection between the primary research when looking at particular situations that indicate how the current technique meets the convergence requirements. In addition, the physical relevance of the contributed parameters is shown via graphs and tables. The discovery demonstrates that an increase in the particle concentration of the hybrid nanofluid accelerates the flow of the fluid. In addition, factoring in dissipative heat makes it more likely that the fluid temperature will be increased to accommodate the participation of the particle concentration.

Interstitial flow, pressure and residual stress in the aging carotid artery model in FEBio.

Vascular smooth muscle cells (VSMCs) are subject to interstitial flow-induced shear stress, which is a critical parameter in cardiovascular disease progression. Transmural pressure loading and residual stresses alter the hydraulic conductivity of the arterial layers and modulate the interstitial fluid flux through the arterial wall. In this paper, a biphasic multilayer model of a common carotid artery (CCA) with anisotropic fiber-reinforced soft tissue and strain-dependent permeability is developed in FEBio software. After the verification of the numerical predictions, age-related arterial thickening and stiffening effects on arterial deformation and interstitial flow are computed under physiological geometry and physical parameters. We found that circumferential residual stress shifts outward in each layer and its gradient increases up to 6 times with aging. Internally pressurized CCA displays nonlinear deformation. In the aged artery, the circumferential stress becomes greater on the media layer (82-158kPa) and lower on the intima and adventitia (19-23kPa and 25-28kPa, respectively). The radial compression of the intima reduces the total hydraulic conductivity by 48% in the young and 16% in the aged arterial walls. Consequently, the average radial interstitial flux increases with pressure by 14% in the young and 91% in the aged arteries. Accordingly, the flow shear stress experienced by the VSMCs becomes more significant for aged arteries, which may accelerate cardiovascular disease progression compared to young arteries.

Mechanical analysis and machinability evaluation of a new EDM-turning hybrid machining process

This study developed an accurate electrical discharge-assisted turning (EDAT) technique for Ti–6Al–4 V alloy and a precise EDAT cutting force prediction model considering theoretical multi-physics fields with metallurgical transformation. A heat-fluid coupled model of multi-pulse discharge in the EDAT process was developed to obtain the thickness of the removal layer (RL) and modified layer (ML) together with the temperature field of the cutting layer. Subsequently, the shear flow stresses under different machining parameters were obtained using the oblique cutting and flow softening constitutive models. Within the cutting layer, the pure thermal softening effect reduces the flow stresses by more than 25% in the unmodified matrix layer (UML) and by more than 60% in the ML, which is dominated by the metallurgical transformation softening (MTS) effect. Finally, the cutting force model of EDAT under multi-physical fields was established and the accuracy was verified by relevant experiments. The results showed that the increase in discharge number accelerated the rise of workpiece surface temperatures, and the small cutting parameters were favorable to the machining performance of the EDAT. The MTS effect significantly reduces cutting forces while improving the quality of the machined surface.

Computational fluid dynamics as supporting technology for coronary artery disease diagnosis and treatment: an international survey.

Computational fluid dynamics (CFD) is emerging as an effective technology able to improve procedural outcomes and enhance clinical decision-making in patients with coronary artery disease (CAD). The present study aims to assess the state of knowledge, use and clinical acceptability of CFD in the diagnosis and treatment of CAD. We realized a 20-questions international, anonymous, cross-sectional survey to cardiologists to test their knowledge and confidence on CFD as a technology applied to patients suffering from CAD. Responses were recorded between May 18, 2022, and June 12, 2022. A total of 466 interventional cardiologists (mean age 48.4 ± 8.3 years, males 362), from 42 different countries completed the survey, for a response rate of 45.9%. Of these, 66.6% declared to be familiar with the term CFD, especially for optimization of existing interventional techniques (16.1%) and assessment of hemodynamic quantities related with CAD (13.7%). About 30% of respondents correctly answered to the questions exploring their knowledge on the pathophysiological role of some CFD-derived quantities such as wall shear stress and helical flow in coronary arteries. Among respondents, 85.9% would consider patient-specific CFD-based analysis in daily interventional practice while 94.2% declared to be interested in receiving a brief foundation course on the basic CFD principles. Finally, 87.7% of respondents declared to be interested in a cath-lab software able to conduct affordable CFD-based analyses at the point-of-care. Interventional cardiologists reported to be profoundly interested in adopting CFD simulations as a technology supporting decision making in the treatment of CAD in daily practice.

Computational modeling based on confocal imaging predicts changes in osteocyte and dendrite shear stress due to canalicular loss with aging.

Exercise and physical activity exert mechanical loading on the bones which induces bone formation. However, the relationship between the osteocyte lacunar-canalicular morphology and mechanical stress experienced locally by osteocytes transducing signals for bone formation is not fully understood. In this study, we used computational modeling to predict the effect of canalicular density, the number of fluid inlets, and load direction on fluid flow shear stress (FFSS) and bone strains and how these might change following the microstructural deterioration of the lacunar-canalicular network that occurs with aging. Four distinct computational models were initially generated of osteocytes with either ten or eighteen dendrites using a fluid-structure interaction method with idealized geometries. Next, a young and a simulated aged osteocyte were developed from confocal images after FITC staining of the femur of a 4-month-old C57BL/6 mouse to estimate FFSS using a computational fluid dynamics approach. The models predicted higher fluid velocities in the canaliculi versus the lacunae. Comparison of idealized models with five versus one fluid inlet indicated that with four more inlets, one-half of the dendrites experienced FFSS greater than 0.8Pa, which has been associated with osteogenic responses. Confocal image-based models of real osteocytes indicated a six times higher ratio of canalicular to lacunar surface area in the young osteocyte model than the simulated aged model and the average FFSS in the young model (FFSS = 0.46Pa) was three times greater than the aged model (FFSS = 0.15Pa). Interestingly, the surface area with FFSS values above 0.8Pa was 23 times greater in the young versus the simulated aged model. These findings may explain the impaired mechano-responsiveness of osteocytes with aging.

Blood Flow with Heat Transfer through Different Geometries of Stenotic Arteries

The current study evaluates blood flow patterns in symmetrical, elliptical, trapezoidal, triangular, cosine-shaped and bell-shaped stenosed arteries with heat transfer. The ordinary differential equations obtained from the transformation applicable to the controlling partial differential equations. The system of ordinary differential equations has been solved using analytical methods of variation of parameters with the help of software. The MATLAB code graphically demonstrates various profiles and analyses the impact of the Prandtl number, magnetic field, Darcy number and Radiation number on velocity, wall shear stress, temperature and volumetric flow rate. The preponderance of blood flow problems is caused by stenosis between z = 1 and z = 2 locations for symmetrical, elliptical, trapezoidal, triangular, cosine-shaped and bell-shaped stenosis. The originality of the present study is the simultaneous influence of varied stenosis geometries on heat transfer. The main observation of blood velocity is that it is also greater for bell-shaped artery walls in the presence of a magnetic field than for trapezoidal artery walls between z = 1 and z = 2 and all other geometries in between. It may help to understand the blood flow in stenosed blood arteries caused by cardiovascular disease. HIGHLIGHTS The current study evaluates blood flow patterns in symmetrical, elliptical, trapezoidal, triangular, cosine-shaped and bell-shaped stenosed arteries with heat transfer The effect of magnetic field and Darcy number on a diseased segment of an artery is examined Due to the presence of hemoglobin in RBCs, which contains iron oxide particles and is capable of binding oxygen molecules, the blood is greatly impacted by the magnetic field GRAPHICAL ABSTRACT

Effects of size and shape of the side holes of a double J stent on the ureter fluid flow after stenosis

The effect of side holes morphology changes in double J stent (DJS) on encrustation was analyzed using computational fluid dynamics (CFD). We analyzed DJS side holes with inner diameter of 1 mm and outer diameters of 1 (type A), 1.2 (type B) and 1.4 (type C) mm, respectively. Concentric stenosis with three intraureteral degree (0%, 12%, and 88%) was analyzed. The flow rate, shear stress and wall shear stress (WSS) distribution were investigated. Urine flow through SH1 before the ureteropelvic junction (UPJ) differed based on the ureteral stenosis degree. The sum of flow rates through the SHs increased with diameter. In the stented ureter with 12% stenosis, the flow rate through SH1 approximately doubled than that without ureteral stenosis, and the flow rate through SH1 was maximal for the type ‘C’ stent in both 12% and 88% ureteral stenosis. The mean shear stress in the SHs increased with the degree of stenosis. The WSS around the SHs was higher for type ‘C’ than types A and B. From the flow rates and shear stresses in and around the SHs, the larger SH diameter of the DJS from the UPJ to mid-ureter is expected to induce encrustation reduction, especially in patients with urinary lithiasis.

Numerical simulation of the cavopulmonary connection flow with conduit stenoses of varying configurations

The circulation in the total cavopulmonary connection (TCPC) is a low-energy system which operation and efficiency are subjected to multiple factors. Some retrospective studies report that the abnormal narrowing of vessels in the system, i.e. stenosis, is one of the most dangerous geometric factors which can result in heart failure.In the present study, the effect of varying extracardiac conduit (ECC) stenosis on the hemodynamics in a surrogate TCPC model is investigated using high-fidelity numerical simulations. The efficiency of the surrogate TCPC model was quantified according to the power loss, relative perfusion in lungs and the percentage of conduit surface area with abnormally low and high wall shear stress for venous flow. Additionally, the impact of respiration and asymmetry in the stenosis geometry to the system was examined. The results show that the flow in the TCPC model exhibits pronounced unsteadiness even under the steady initial boundary conditions, while the uneven pulmonary flow distribution and the presence of the ECC stenosis amplify the chaotic nature of the flow. Energy efficiency of the system is shown to strongly correlate with amount of vortical structures in the model and their range of scales. Finally, the study demonstrates that the presence of respiration in the model adds to perturbations in the flow which causes increase in the power loss. Results obtained in the study provide valuable insights on how the ECC stenosis effect the flow in the surrogate TCPC model under different flow conditions.

Biomechanical Investigation of Red Cell Sedimentation Using Blood Shear Stress and Blood Flow Image in a Capillary Chip.

Blood image intensity has been used to detect erythrocyte sedimentation rate (ESR). However, it does not give information on the biophysical properties of blood samples under continuous ESR. In this study, to quantify mechanical variations of blood under continuous ESR, blood shear stress and blood image intensity were obtained by analyzing blood flows in the capillary channel. A blood sample is loaded into a driving syringe to demonstrate the proposed method. The blood flow rate is set in a periodic on-off pattern. A blood sample is then supplied into a capillary chip, and microscopic blood images are captured at specific intervals. Blood shear stress is quantified from the interface of the bloodstream in the coflowing channel. τ0 is defined as the maximum shear stress obtained at the first period. Simultaneously, ESRτ is then obtained by analyzing temporal variations of blood shear stress for every on period. AII is evaluated by analyzing the temporal variation of blood image intensity for every off period. According to the experimental results, a shorter period of T = 4 min and no air cavity contributes to the high sensitivity of the two indices (ESRτ and AII). The τ0 exhibits substantial differences with respect to hematocrits (i.e., 30-50%) as well as diluents. The ESRτ and AII showed a reciprocal relationship with each other. Three suggested properties represented substantial differences for suspended blood samples (i.e., hardened red blood cells, different concentrations of dextran solution, and fibrinogen). In conclusion, the present method can detect variations in blood samples under continuous ESR effectively.

Bilayer vascular grafts with on-demand NO and H2S release capabilities

Nitric oxide (NO) and hydrogen sulfide (H2S) gasotransmitters exhibit potential therapeutic effects in the cardiovascular system. Herein, biomimicking multilayer structures of biological blood vessels, bilayer small-diameter vascular grafts (SDVGs) with on-demand NO and H2S release capabilities, were designed and fabricated. The keratin-based H2S donor (KTC) with good biocompatibility and high stability was first synthesized and then electrospun with poly (l-lactide-co-caprolactone) (PLCL) to be used as the outer layer of grafts. The electrospun poly (ε-caprolactone) (PCL) mats were aminolyzed and further chelated with copper (II) ions to construct glutathione peroxidase (GPx)-like structural surfaces for the catalytic generation of NO, which acted as the inner layer of grafts. The on-demand release of NO and H2S selectively and synergistically promoted the proliferation and migration of human umbilical vein endothelial cells (HUVECs) while inhibiting the proliferation and migration of human umbilical artery smooth muscle cells (HUASMCs). Dual releases of NO and H2S gasotransmitters could enhance their respective production, resulting in enhanced promotion of HUVECs and inhibition of HUASMCs owing to their combined actions. In addition, the bilayer grafts were conducive to forming endothelial cell layers under flow shear stress. In rat abdominal aorta replacement models, the grafts remained patency for 6 months. These grafts were capable of facilitating rapid endothelialization and alleviating neointimal hyperplasia without obvious injury, inflammation, or thrombosis. More importantly, the grafts were expected to avoid calcification with the degradation of the grafts. Taken together, these bilayer grafts will be greatly promising candidates for SDVGs with rapid endothelialization and anti-calcification properties.

A Computational Fluid Dynamics-Based Model for Assessing Rupture Risk in Cerebral Arteries with Varying Aneurysm Sizes

A cerebral aneurysm is a medical condition where a cerebral artery can burst under adverse pressure conditions. A 20% mortality rate and additional 30 to 40% morbidity rate have been reported for patients suffering from the rupture of aneurysms. In addition to wall shear stress, input jets, induced pressure, and complicated and unstable flow patterns are other important parameters associated with a clinical history of aneurysm ruptures. In this study, the anterior cerebral artery (ACA) was modeled using image segmentation and then rebuilt with aneurysms at locations vulnerable to aneurysm growth. To simulate various aneurysm growth stages, five aneurysm sizes and two wall thicknesses were taken into consideration. In order to simulate realistic pressure loading conditions for the anterior cerebral arteries, inlet velocity and outlet pressure were used. The pressure, wall shear stress, and flow velocity distributions were then evaluated in order to predict the risk of rupture. A low-wall shear stress-based rupture scenario was created using a smaller aneurysm and thinner walls, which enhanced pressure, shear stress, and flow velocity. Additionally, aneurysms with a 4 mm diameter and a thin wall had increased rupture risks, particularly at specific boundary conditions. It is believed that the findings of this study will help physicians predict rupture risk according to aneurysm diameters and make early treatment decisions.