Flotation Method Research Articles

Testing of the supramolecular complex of ivermectin Aniverm-2.0% against parasitosis on various types of livestock animals

The purpose of the research is to obtain and test a complex drug Aniverm-2.0% based on the ivermectin substance by free feeding in a mixture with concentrated feed against parasitosis of cattle, sheep, and horses.Materials and methods. Aniverm-2.0% obtained by mechanochemical technology was tested on a farm in the Shatoy Region, the Chechen Republic, on 900 sheep, 180 cattle and 156 horses in March-April 2023. To determine the infection rate, coproovoscopic examinations were conducted on 80 samples from sheep, 20 samples from young cattle and 30 samples from horses using the Fulleborn’s flotation method with a saturated sodium chloride solution. Sheep were dewormed using a group method. The drug was mixed with feed per 0.3 mg/kg for the AS or 60 mg for the drug per animal. The young cattle were kept by tie-up housing and therefore they were given Aniverm-2.0% once at a dose of 0.2 mg/kg for the AS individually in a mixture with feed. The horses were given the drug in a group method at a dose of 0.2 mg/kg for the AS or 1.5 mg/kg for the drug. Fecal samples from all animals were examined before and 2 weeks after the drug.Results and discussion. A 100% rate of infection of the sheep by gastrointestinal nematodes (Strongylata, Nematodirus spp., Trichocephalus spp.) was determined with an average number of eggs 633.3 in 1 g of feces. In 20 fecal samples from the young cattle, 16 were found to have Strongylata eggs (prevalence = 80.0%) with 222.2–280.5 specimens of Strongylata eggs found in 1 g of feces. Strongylata eggs were found in all 30 examined fecal samples from the horses, on average, 253.3 specimens/g of feces (100%). At 2 weeks after treatment, nematode eggs were found in three out of 80 examined fecal samples from the sheep, which can be explained that the sheep did not eat the appropriate dose of the drug. The drug efficacy was determined to be 99.7%. One hundred percent efficacy of the drug was obtained on young cattle and horses.

Investigation of Head Lice and Intestinal Parasites in Primary and Secondary School in Sivas.

The purpose of this study was to investigate the prevalence of intestinal parasites and head lice in primary school-aged children and to determine a possible relationship between the co-occurrence of ecto-endoparasites. The study were conducted in three schools in the center of Sivas: Sivas Karşıyaka Primary School, Kılavuz İMKB Primary School, and Karşıyaka 27 June Middle School. The presence of head lice and intestinal parasites was investigated in a total of 563 children aged 6 to 17, with 294 girls and 269 boys. Classroom teachers and students were provided with information about intestinal parasites, head lice, and methods of transmission and prevention, and a questionnaire was administered. During the head lice examination, students were inspected with a comb, and samples with nits were examined under a microscope in the laboratory. Cellophane tape and stool samples obtained from the participants were examined on the same day using the native-lugol, flotation, and trichrome staining methods under a light microscope (x10, x40, x100). Five hundred sixty-three children were evaluated for head lice and 15 (2.6%) were found to be positive. During the examination, only nits were found in 11 students (1.9%), and both adult lice and nymphs were seen in 4 students (0.7%). Head lice were found positive in 2 out of 269 boys (0.7%), while 13 out of 294 girls (4.2%) positive (p<0.05). As a result of stool examination in children, Entamoeba coli was found in 11 (1.95%), Giardia intestinalis in 4 (0.71%), Blastocystis sp. in 14 (2.48%), Enterobius vermicularis in 8 (1.42%) and Taenia spp. in 2 (0.35%). Head lice infestation and Taenia spp. were found to coexist in 2 (0.35%) children. In conclusion, although there has been a noticeable decrease in head lice and intestinal parasites among primary school-aged children in Sivas province, these conditions are still present, as evidenced by previous studies conducted in our region.

Outcome of parasitological examinations in dogs in Germany: a retrospective survey

Dog faecal samples examined from January 2019 to December 2019 were retrospectively analysed for frequency of endoparasites. The examinations were performed with several different methods: 29,219 samples were examined by flotation method and sodium acetate-acetic acid-formalin concentration (SAFC) technique, 1,330 samples by Baermann-Wetzel migration technique, 12,221 samples using a Giardia coproantigen enzyme-linked-immunosorbent assay (ELISA), 1,180 samples using a Cryptosporidium coproantigen ELISA, 1,671 samples by polymerase chain reaction (PCR) testing for Giardia duodenalis and 447 samples by PCR testing for Cryptosporidium spp.. A total of 7.1% of the samples were positive for parasites in the microscopical examination using the flotation method and SAFC technique. The parasites found included Cystoisospora spp. (2.8%), Giardia duodenalis (2.3%), Ancylostomatidae (1.8%), Toxocara canis (1.6%), Trichuris vulpis (0.7%), Toxascaris leonina (0.5%), Capillaria spp. (0.2%), Angiostrongylus vasorum (0.2%), Crenosoma vulpis (0.1%), Taeniidae (0.1%), Sarcocystis spp. (0.03%), Dipylidium caninum (0.01%), Diphyllobothrium latum (< 0.01%), Spirurida (< 0.01%) and Opisthorchiidae (< 0.01%). Using the Baermann-Wetzel migration technique, Angiostrongylus vasorum was found in 0.75% and Crenosoma vulpis in 0.3% of the samples. ELISAs for Giardia duodenalis and Cryptosporidium spp. revealed 13.9% and 1.0% positive faecal samples, and Giardia duodenalis and Cryptosporidium spp. PCRs 19.4% and 2.0%, respectively. Dogs in the first year of life were more frequently infected with parasites than older animals. In the microscopic examination using flotation method and SAFC technique, the significantly highest detection rates were found in dogs up to six months of age (p < 0.001).

Highly selective ion precipitation flotation for ternary Co–Zn–Mn separation: Stepwise chelation capture of Co and Zn from simulated zinc hydrometallurgy wastewater

Ion precipitation flotation technology was demonstrated to be an efficient method for the separation of valuable metals from low-concentration solution. However, the selective separation of three metals from mixing solution is a great challenge, and highly selective reagents are the key to polymetallic separation. In this work, stepwise separation of Co and Zn from the simulated zinc hydrometallurgy wastewater containing ternary Co–Zn–Mn metals by ion precipitation flotation process was proposed. It's demonstrated that organic reagents of 1-nitroso-2-naphthol (NN) and sodium dimethyldithiocarbamate (SDDC) had excellent selectivity for the capture of Co and Zn to form respective precipitate from wastewaters via the chelation reactions. After precipitation, dodecylpyridinium chloride (DPC) and tetradecyltrimethylammonium bromide (TTAB) were chosen as surfactants for the separation of Co and Zn sediments from the solution via the flotation process. The effects of solution pH, molar ratio, reaction temperature, and reaction time on the selective precipitation efficiencies of Co and Zn as well as the effects of surfactant dosage and flotation gas velocity on the flotation separation efficiencies were systematically investigated. It's demonstrated that the comprehensive recovery rates of Co, Mn, and Zn reach 98%, 90%, and 99%, respectively. After separation, oxidation calcination of the foam products was conducted to prepare high-purity Co3O4 and ZnO nanoparticles in which the organic matters were burnt out with gas emissions. The stepwise chelation capture mechanisms of Co and Zn by highly selective precipitation reagents were minutely discussed. It's demonstrated that the proposed selective stepwise precipitation and flotation method is suitable for recovery of critical metal ions from low-concentration polymetallic wastewaters.

Occurrence of Eimeria spp. and Intestinal Helminths in Free-Range Chickens from Northwest and Central Romania.

Chickens raised in backyard free-range systems are confronted with a significant threat of parasitic infections. Among the parasitic agents, protozoa belonging to the genus Eimeria and helminths, including Ascaridia galli, Capillaria spp., Heterakis gallinarum, and Strongyloides avium, stand out as the most prevalent. The sampling protocol included sixteen localities in four counties within the Transylvania region of Romania. Fecal samples were collected from chickens reared in a backyard system. Fecal samples were screened for oocysts (O) and eggs (E) by flotation method, and their number per gram of feces (OPG/EPG) was calculated after counting them by McMaster method. Positive samples for Eimeria spp. were further analyzed by PCR (polymerase chain reaction) method to identify the Eimeria species. A total of 145 flocks were tested and the overall prevalence of infection was 53.1%. The most prevalent infections were with A. galli/H. gallinarum (25.5%), and Eimeria spp. (24.8%), followed by Capillaria spp. and strongyles. The mean OPG/EPG values were as follows: 63,577 for Eimeria spp., 157 for Ascaridia/Heterakis, 362 for Capillaria spp., and 1671 for Strongyle eggs. Identified Eimeria species were E. acervulina (41.7%), E. tenella (27.8%), E. praecox (16.7%), E. brunetti (16.7%), OTUy (operational taxonomic unit y) (8.3%), OTUz (operational taxonomic unit z) (8.3%) and E. mitis (5.6%). Intestinal parasites exhibit a high prevalence among chickens in backyard poultry flocks, and the presence of significant parasite burdens can adversely affect both productive and economic aspects. To the best of our knowledge, this is the first comprehensive study that aimed to analyze the prevalence of gastrointestinal parasites in chickens raised in a backyard free-range system in Romania, and the first report of OTUy species in Europe.

Assessment of Ovbiomu Coal for Industrial Application

This research is to assess the quality of Ovbiomu lignite coal deposited for optimum utilization in metallurgical industries in Ovbiomu. Samples of coal were collected for analysis and sample was collected from five different stationary lots until 10 kilograms of the sample was collected and 500g was taken for characterization. The sample was reduced using jaw crusher and ball milled to a size of 1400μm and 1 kilograms of 1400μm was further reduced to 1100μm and was classified into various sieve sizes using mechanical sieve shaker. CalK2 bomb calorimeter was used to determine the calorific value of the head sample, the economic liberation size and the actual liberation size. The proximate analysis of the head sample and all the sieve sizes was done using Furnace, oven, porcelain crucibles, analytical balance, and desiccator to ascertain the individual carbon content. Ultimate analysis was done using XRD and concentration was done using froth flotation method. Result from sieve analysis shows that at 1000 μm, 710 μm, 500 μm, 355 μm, 250 μm, 180 μm, 25 μm, 90 μm, 63 μm, and -63 μm, the following weight was retained 1.45 g 1.86 g, 3.73 g, 3.5 g, 14.61 g, 48.28 g, 0.15 g, 0.17 g and 0.20 g respectively. The economic liberation size was found out to be 180um where most of the sample is retained and the actual liberation size was found out to be 125um but with a very small quantity retained. The results of the calorific value of the head sample and each of the sieve sizes of 250μm, 180μm, and 125μm, was determined to be 24.51MJ/Kg, 25.86MJ/Kj, 18.57MJ/Kg and 38.07MJ/Kg respectively, the following are result of percentage carbon content, for the Head sample (40.65%), 1000μm (29.55%), 710μm (38.53%), 500μm (42.43%), 335μm (35.42%), 250μm(43.07%), 180μm (30.92),125μm (63.40%) 90μm (0.56%) 63μm (0.62%) and -63μm (9.91%). The result of ultimate analysis of the sample shows Nitrogen (1.15%), Hydrogen (4.80%), Sulphur (0.13%) and Oxygen (29.56%).

Comparison of structural effects of cholesterol, lanosterol, and oxysterol on phospholipid (POPC) bilayers

Membrane sterols contribute to the function of biomembranes by regulating the physical properties of the lipid bilayers. Cholesterol, a typical mammalian sterol, is biosynthesized by oxidation of lanosterol. From a molecular evolutionary perspective, lanosterol is considered the ancestral molecule of cholesterol. Here, we studied whether cholesterol is superior to lanosterol in regulating the physical properties of the lipid bilayer in terms of the structural effect on model biomembranes composed of a phospholipid. For comparison, oxysterol, which is formed by oxidation of cholesterol, was also studied. The phospholipid used was 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), which is abundantly found in mammalian biomembranes, and 7β-hydroxycholesterol, which is highly cytotoxic, was used as the oxysterol. The apparent molecular volume was calculated from the mass density determined by the flotation method using H2O and D2O, and the bilayer thickness was determined by reconstructing the electron density distribution from X-ray diffraction data of the POPC/sterol mixtures at a sterol concentration of 30 mol%. The apparent occupied area at the bilayer surface was calculated from the above two structural data. The cholesterol system had the thickest bilayer thickness and the smallest occupied area of the three sterols studied here. This indicates that the POPC/cholesterol bilayer has a better barrier property than the other two systems. Compared to cholesterol, the effects of lanosterol and 7β-hydroxycholesterol on lipid bilayer properties can be interpreted as suboptimal for the function of mammalian biomembranes.

Study on the Purification Effect and Mechanism of Micro–Nano-Bubble-Based Two-Stage Air Floatation Method on the Raw Sugar Redissolved Syrup

Study on the Purification Effect and Mechanism of Micro–Nano-Bubble-Based Two-Stage Air Floatation Method on the Raw Sugar Redissolved Syrup

Selective separation of anglesite from iron ore sintering Dust: A novel aggregate flotation method

Iron ore sintering dust, a solid waste produced during steel manufacturing, contains high levels of lead and iron compounds, particularly anglesite and hematite. Traditional sulfidation flotation methods struggle to effectively separate these fine-grained minerals. This study introduces a selective aggregate flotation method that enhances selectivity through a synergistic use of sodium lauryl sulfate (SLS) and sodium tripolyphosphate (STPP). The results from flotation experiments show a remarkable reduction in hematite recovery to 7.3% from 19.0%, while maintaining a high recovery of over 90% for anglesite, yielding a more efficient separation. Analytical tests such as grading analysis, zeta potential, FT-IR and XPS analysis observed that SLS’s ability to chemically adsorb onto the lead ions in anglesite aids in the aggregation of these particles, enhancing their recovery. In contrast, STPP adsorbs onto iron ions on the hematite surface, preventing SLS from adhering to it and thus promoting the dispersion of hematite particles. This differential chemical interaction creates significant physical differences between anglesite and hematite, enabling their effective separation during beneficiation. Overall, this innovative approach provides new ideas for an efficient strategy to recover precious metals from sintering dust.

Multi-scale habitat modeling framework for predicting the potential distribution of sheep gastrointestinal nematodes across Iran’s three distinct climatic zones: a MaxEnt machine-learning algorithm

Ecological niche models (ENMs) serve as valuable tools in assessing the potential species distribution, identifying crucial habitat components for species associations, and facilitating conservation efforts. The current study aimed to investigate the gastrointestinal nematodes (GINs) infection in sheep, predict and analyze their ecological niches and ranges, and identify the key bioclimatic variables influencing their distribution across three distinct climatic regions in Iran. In a cross-sectional study, a total of 2140 fecal samples were collected from semi-arid (n = 800), arid (n = 500), and humid-subtropical (n = 840) climates in East Azerbaijan, Kerman, and Guilan provinces, respectively. The flotation method was employed to assess stool samples, whereby the fecal egg count (the number of parasite eggs per gram [EPG]) was ascertained for each individual specimen. Employing a presence-only approach, the multi-scale maximum entropy (MaxEnt) method was used to model GINs' habitat suitability using 93 selected points/locations. The findings revealed that Guilan (34.2%) and East Azerbaijan (19.62%) exhibited the utmost proportion of Strongyle-type eggs. East Azerbaijan province also displayed the highest proportion of Marshallagia and Nematodirus, respectively (approximately 40% and 27%), followed by Guilan and Kerman provinces, while Kerman province had the highest proportion of Trichuris (approximately 15%). Ecological niche modeling revealed that the precipitation of the driest quarter (Bio17) exerted the most significant influence on Marshallagia, Nematodirus, Trichuris, and ُSُُُtrongyle-type eggs' presence in East Azerbaijan and Kerman provinces. For Guilan province, the most influential factor defining habitat suitability for Strongyle-type eggs, Marshallagia, and Nematodirus was increasing slope. Additionally, the distribution of Trichuris was most affected by the variable Bio2 in Guilan province. The study highlights the response of GINs to climate drivers in highly suitable regions, providing insights into ecologically favorable areas for GINs. In conclusion, this study provides a better understanding of GINs and the environmental factors influencing their transmission dynamics.

Prevalence and diversity of gastrointestinal parasites on pig farms in Kalasin Province, Thailand.

Gastrointestinal (GI) parasite infections are the foremost and prevalent diseases that affect pigs in Thailand. This study aimed to determine the prevalence of GI parasites among pigs in Kalasin Province. This study is the first attempt to understand the occurrence of GI parasites in pigs within Kalasin province using fecal samples as the methodology. We collected 324 fecal samples directly from the rectums of pigs from May to July 2023. The formalin-ethyl acetate concentration and floatation methods were used to examine these samples. The eggs or oocysts were identified by observing their morphology and size under a light microscope. Of the 324 fecal samples examined, 276 tested positive for gastrointestinal parasitic infections, resulting in a prevalence rate of 85.19%. Nematodes were the most prevalent, with Strongyle-type infections being the highest in 267 cases (82.41%), followed by Ascaris suum in 222 cases (68.52%), and Trichuris spp. in 152 cases (46.91%). Strongyloides spp. infections were also observed in 92 cases (28.40%). However, trematode infection was relatively rare, with only Fasciola spp. found in 15 cases (4.63%). We identified Eimeria spp. in 87 cases (26.85%), Iodamoeba spp. in 70 cases (21.60%), Balantidium coli in 67 cases (20.68%), and Isospora spp. in 52 cases (16.05%). Notably, most of the positive fecal samples showed double infections with a prevalence rate of approximately 38.27%. Single infections were the next most common, accounting for 25.31% of the cases, followed by 3 parasites (14.81%) and 4-5 parasites (6.79%). This study underscores the high endemicity of GI parasites among pigs in Kalasin province. To improve prevention and control measures, it is recommended to establish a health monitoring program that includes deworming and emphasizes good hygiene practices. The insights gained from this study will contribute to the enhancement of pig farming practices in Kalasin province, ultimately leading to improved production and profitability. In addition, future research should focus on detecting these parasites in Kalasin and exploring their relationship with human transmission cycle.

Studying Flotation of Gold Microdispersions with Carrier Minerals and Pulp Aeration with a Steam–Air Mixture

This work is aimed at obtaining new knowledge in the field of interactions of polydisperse hydrophobic surfaces in order to increase the extraction of mineral microdispersions via flotation. The effect of high velocity and the probability of aggregating fine particles with large ones are used to increase the extraction of finely dispersed gold in this work. Large particles act as carrier minerals, which are intentionally introduced into a pulp. The novelty of this work lies in the fact that a rougher concentrate is used as the carrier mineral. For this purpose, it is isolated from three parallel pulp streams by mixing the rougher concentrate, isolated from the first stream of raw materials, with an initial feed of the second stream; accordingly, the rougher concentrate of the second stream is mixed with the initial feed of the third stream, and the finished rougher concentrate is obtained. In this mode of extracting the rougher concentrate, the content of the extracted metal increases from stream to stream, which contributes to the growth in its content in the end product. Moreover, in order to supplement forces involved in the separation of minerals with surface forces of structural origin in the third flotation stream, the pulp is aerated for a short time (about 15%–25% of the total) with air bubbles filled with a heat carrier, i.e., hot water vapor. Within this accepted flotation method, the influence that the surface currents occurring in the wetting film have on its thinning and breakthrough kinetics is proposed to be in the form of a correction to a length of a liquid slip in the hydrophobic gap. The value of the correction is expressed as a fraction of the limiting thickness of the wetting film, determined by the condition of its thickness invariability when the streams are equal in an interphase gap: outflowing (due to an action of the downforce) and inflowing (Marangoni flows and a thermo-osmotic stream). Gold flotation experiments are performed on samples of gold-bearing ore obtained from two deposits with conditions that simulate a continuous process. Technological advantages of this developed scheme and a flotation mode of gold microdispersions are shown in comparison with the basic technology. The purpose of this work is to conduct comparative tests on the basic and developed technologies using samples of gold-bearing ore obtained from the Natalka and Olimpiada deposits. Through the use of the developed technology, an increase in gold extraction of 7.99% and in concentrate quality (from 5.09 to 100.3 g/t) is achieved when the yield of the concentrate decreases from 1.86 to 1.30%, which reduces the costs associated with its expensive metallurgical processing.

Identification of eggs of soil transmitted helminthes (STH) intestinal nemathodes in children by flotation method in Tengket village, Arosbaya district, Bangkalan regency

Background: Worm infections can occur in humans throughout the world regardless of age and attack children's health. This statement encouraged researchers to do this in Tengket Village, where most guardians ignore their children's PBHS and latrines in rivers around residents' houses cause worm infections. More than 1.5billion people, or 24% of the world's population, are infected with soil-borne worms. That are transmitted through the soil are also called nematodes of the STH class. Objectives: The study aimed to identify STH intestinal nematode eggs in children in Tengket Village, Arosbaya, Bangkalan. Materials and Methods: The method used is flotation using NaCl reagent with the principle of different specific gravity so the parasites in the feces will be floated on the surface. The floatation method is able to produce a clean field of view and an easy procedure, thereby minimizing false positives and false negatives. Results: The research was carried out in February 2023 and the results obtained were that 9 out of 30 children's feces samples tested positive for worms. The positive results in the samples could be due to river latrines still being used and the spread occurring due to lack of attention to PHBS. Conclusions: Based on the research results, the percentage of worms has increased.

Toxascaris leonina in dogs – A nematode species of high prevalence in some regions of Eurasia

A recent meta-analysis of studies from around the world had shown a global prevalence of Toxascaris leonina in stray dogs of about 7%. However, studies from Eurasian regions, where higher percentages of positive dogs were often found, were under-represented in this meta-analysis. This prompted the present study. Its main objective was to examine free-roaming dogs from the capital city of Kazakhstan to obtain information on the current occurrence of T. leonina egg shedding compared to Toxocara spp.Faecal samples from 500 free-roaming dogs from the city of Astana and its suburbs were collected 1–2 days after admission to the municipal animal shelter during three time periods (May to October 2019, September 2020, and April 2021). Samples were examined by a saline flotation method. Nematode egg-positive samples were subjected to a modified McMaster method to determine the number of eggs per gram of faeces (EPG).Toxascaris leonina eggs were found in 53.8% and Toxocara spp. eggs in 14.8% of the dogs examined. The prevalence of T. leonina egg shedding was significantly associated with age class but not with sex: dogs aged between 6 months and 2 years were more likely to be T. leonina egg positive than puppies. The intensity of T. leonina egg shedding was also age dependent: dogs aged between 6 months and 2 years had significantly higher EPGs compared to younger or older animals. In contrast, the prevalence of Toxocara spp. egg shedding was associated with both age class and sex: dogs older than 2 years were less likely to shed Toxocara spp. eggs than puppies, and females were less likely to be Toxocara spp. egg positive compared to males.The present results confirm those of other studies in Eurasian regions. It is therefore reasonable to assume that the global prevalence of T. leonina infection has been underestimated and should be revised accordingly.

Preparation of feedstock for potassium chloride granulation plants

The assessment of existing technological solutions in the field of preparation of feedstock for potassium chloride granulation plants of silvinite processing plants of JSC “Belaruskali”, compliance of these solutions with the developed theoretical concepts and recommendations was carried out. It has been established that in the granulation departments of factories with the flotation method of enrichment at the stage of homogenization of the charge by granulation composition and humidity, and the improvement of its crystal structure, the process of particle agglomeration is not efficient enough due to the technical imperfection of the used agglomerators. At the same time, there is a significant overspending of the structure-forming reagent compared to the amount calculated in accordance with the stoichiometry of calcium and magnesium ions, which leads to excessive contamination of the finished product with sodium carbonate. To eliminate the identified drawback, it is proposed to use as an agglomeration device a turbo-blade agglomerator mixer of the TLA-080 brand manufactured by the JSC “Soligorsk Institute of Resources Saving Problems with Pilot Production”, specially designed for agglomeration of potassium chloride. It has been established that an increase in the physico-mechanical and physico-chemical characteristics of granulated potassium chloride of a processing plant with a halurgic enrichment method can be achieved by introducing the developed technology of structural agglomeration of the charge entering the granulation plants in turbo-bladе agglomeration mixers of the TLA-080 brand without the use of structure-forming reagents.

TRICHURIOSIS INFECTION AND COCCIDIOSIS IN LANDRACE PIGS

A one-month-old female Landrace pig died after being suspected of being infected with nematode worms and protozoa parasites. The purpose of this study was to establish a diagnosis of death from case pigs. The pigs were taken from a close house farm owned by Mr. Wayan Suastika in Payangan village, Gianyar, with a total population of 20 and had never been dewormed. Case pigs showed symptoms such as anorexia, experiencing blackish diarrhea mixed with a little blood, no appetite, and pale mucosa. The case pig died one day after being taken from the farm, and was necropsied on November 18, 2022 at the Veterinary Pathology Laboratory, Faculty of Veterinary Medicine, Udayana University. Organs that underwent changes such as the brain, trachea, lungs, heart, spleen, liver, kidneys and intestines were taken to the Veterinary Pathology Laboratory for histopathological examination. Examination of feces using the flotation method and the Mcmaster method, as well as making permanent preparations to identify worms were carried out at the Veterinary Parasitology Laboratory. The results of post-mortem examination found typical anatomic pathology in the form of haemorrhagic colon, necrosis and catarrhal or mucohemorrhagic enteritis with mucus, fresh blood and/or necrotic debris in the lumen, along with infection with nematode worms in the colon with a total of 235 individuals. From the results of stool examination, Trichuris suis worm eggs and Eimeria spp. oocysts were found. Based on the results of pathological and parasitological examinations, it can be concluded that cases of death in pigs are caused by Trichuriosis and Coccidiosis. As a suggestion, pigs should be kept in cages with floors made of concrete or cement. If an earthen yard is used, excreta disposal should be regular and the manure disposal site should be made of closed concrete wells so as not to attract beetles.

Haemonchosis in Small Ruminants Under Traditional Husbandry Systems in Apac District, Northern Uganda.

Haemonchus contortus is one of the most common and fatal pathogenic gastrointestinal nematodes of small ruminants causing significant economic losses, particularly in low-income countries. A cross-sectional study was carried out in randomly selected small ruminants kept under traditional husbandry systems in the Apac district (now split into Apac and Kwania districts) in northern Uganda to assess the prevalence, intensity, and associated risk factors of H. contortus infection from May 2018 to January 2019. Faecal samples were collected from a total of 768 randomly selected small ruminants (384 sheep and 384 goats) and examined for parasites using the floatation method and coproculture. The overall prevalence of H. contortus in small ruminants was 73.3% (563/768) by faecal egg count and 70.6% (542/768) by coproculture. The mean number of eggs per gram of faeces in small ruminants was 2046 ± 107 and differed significantly across the host animals ranging from 1729 ± 120 (mean ± SE) in goats to 2364 ± 176 in sheep. Significant predictors of infection were the origin of study animals, animal species, breed of animal, age of study animal, faecal consistency, lactation, multispecies grazing, grazing method, and anthelmintic use. The findings of our study provide information on the current status of H. contortus infections in goats and sheep under traditional husbandry systems in Uganda which are critical in designing effective control strategies for the disease.

Ash Reduction of the Tailings of Agh-Darband Coal Washing Plant by Flotation Method

Ash Reduction of the Tailings of Agh-Darband Coal Washing Plant by Flotation Method

Prevalence and molecular analysis of Toxocara cati in Baghdad Province.

This study aimed to detect Toxocara cati in cats by microscopic and molecular analysis using PCR, sequencing, and phylogenetic analysis. Randomly selected 200 cat feces samples were taken from various private veterinarian clinics in Baghdad. To identify eggs of T. cati by the flotation method, DNA from 100 cat feces was extracted, and one pair of ITS2 region-specific primers was used for polymerase chain reaction, followed by sequencing. Toxocara cati infection rate was found to be 23 out of 100 fecal samples using PCR. Ten DNA product sequence data studies showed 98%-100% similarity to the 5.8S ribosomal RNA gene sequences found in the Gene Bank. The study incidence showed that the overall infection rate by microscopic examination was 23%, with no significant difference between stray cats (27%), and domestic cats (19%). After studying the effect of several epidemiological parameters on the infection rate, it was found that the infection rates of stray and domestic cats were higher in kittens under six months of age, at 46.1% and 27%, respectively, whereas rates were lower for the adult than six months was 11.5% of domestic cats and 14.7% of stray cats. The percentage of stray and domestic male cats that were registered was 35.5%, whereas the female cats registered were 20.6% and 17.5%, respectively. Cats are significant clinical reservoirs for zoonotic parasites. In Iraq, Baghdad has a high incidence of T. cati detections. Compared to conventional methods, PCR is thought to be a more sensitive, accurate diagnostic procedure that confirms the species' identity.

ВЫБОР ТЕХНОЛОГИИ ПОВЫШЕНИЯ ГИДРОФОБНОСТИ И ИЗВЛЕКАЕМОСТИ АЛМАЗОВ ТРУБКИ "БОТУОБИНСКАЯ"

A promising direction for solving the problem of producing small diamonds is to expand the range of enriched classes and reduce diamond losses through the use of modern technologies. The article presents the results of research on the causes of reduced hydrophobicity of Botuobinskaya tube diamonds and the choice of parameters for preparing the feedstock for the processes of sticky and foam separation, ensuring the restoration of surface properties and extraction of hydrophilized diamonds into concentrate. Studies have shown that the mineral coatings on the surface of diamonds are fragments of primary or modified kimberlites, as well as slurries and films, firmly connected to the surface of diamond crystals. Diamonds from hypergenically altered kimberlites are characterized by the predominance of carbonate and aluminosilicate minerals. It has been shown by the method of unrestricted flotation of diamond-kimberlite samples that the use of mechanical scrubbing is effective enough to remove slurry coatings, but does not provide complete removal of car bonate-aluminosilicate microbreccias firmly bound to the surface of diamonds. As a result of the conducted research, it is shown that for a more complete recovery of small diamonds from kimberlites enriched at the Nyurbinsky GOK, it is advisable to use the processes of mechanical stripping and thermal cleaning of diamonds from hydrophilizing coatings to restore their hydrophobicity. It is shown that the use of a combination of these methods provides an increase in diamond recovery during sticky separation by 14.1 % and during foam separation by 20.4 %. The level of recovery of hydrophilic diamonds (more than 82 %) achieved using foam separation technology allows us to recommend this process for use in improving the technology of enrichment of small classes of diamonds.