Floricultural Plants Research Articles

An assessment of in vitro lead (Pb) bioaccumulation of Dianthus chinensis L. (Chinese pink).

Heavy metals (HM) also known as potentially toxic elements (PTEs), are well-known environmental pollutants, among which lead (Pb) is a widespread and hazardous soil contaminant. Its removal from soil sediments is often difficult to achieve. In this study, in vitro experiments were conducted to investigate the bioaccumulation capability of Dianthus chinensis L. in solid and liquid Murashige and Skoog (MS) medium supplemented with varying concentrations (0, 10, 100, and 200µM) of Pb as lead nitrate [Pb(NO3)2] for 30days. The objectives of the study were to assess the efficiency of the selected plant as a bio-accumulator in the in vitro system and to obtain data on morphological, biochemical, and molecular changes during Pb salt-induced stress. Significant growth patterns of initial growth promotion up to 100µM lead nitrate supplemented medium were observed, with maximum shoot length and biomass production along with remarkable lead bioaccumulation. Molecular studies on in vitro raised plantlets confirm the high degree of genetic uniformity (98.3%) of the selected plants after a considerable duration (30days) of Pb exposure. Biochemical parameters revealed significant stress effects, including a 284% reduction in total chlorophyll content, altered carotenoid, and proline level during the study. The experiment revealed the high tolerance capacity of D. chinensis to Pb salt and its bioaccumulation potential (397.33mg/kg). This increases the possible use of such an ornamental and floriculture plant as a prospective candidate for the efficient removal of soil Pb pollutants, as they can remediate soils, coupled with aesthetic and profitable outcomes for the growers.

Overexpression of acdS in petunia reduces ethylene production and improves tolerance to heat stress.

Petunia hybrida, widely grown as a bedding plant, has reduced growth and flower quality at temperatures above 30 °C (heat stress), primarily due to heat stress-induced ethylene (ET) production. The gene acdS encodes the 1-aminocyclopropane-1-carboxylate (ACC) deaminase (ACCD) enzyme, which is known for its role in reducing ET production by breaking down the ET precursor, ACC, in plant tissues. This study investigated the impact of heat stress on both 'Mirage Rose' WT petunia and its acdS-overexpressing transgenic lines. Heat stress-induced growth inhibition was observed in WT plants but not in transgenic plants. The increased stress tolerance of transgenic plants over WT plants was associated with lower ET production, ROS accumulation, higher SPAD values, water content, and relative water content. Furthermore, higher sensitivity of the WT to heat stress than the transgenic plants was confirmed by analysing ET signalling genes, heat shock transcription factor genes, and antioxidant- and proline-related genes, more strongly induced in WT than in transgenic plants. Overall, this study suggests the potential application of acdS overexpression in other floriculture plants as a viable strategy for developing heat stress-tolerant varieties. This approach holds promise for advancing the floricultural industry by overcoming challenges related to heat-induced growth inhibition and loss of flower quality.

A review on phytoremediation capability of Tagetes erecta Linn. against heavy metals

Now a days, Phytoremediation is treated as a set of emerging techniques that use several selected plants to contain, eradicate, immobilize or degrade contaminants from water and soil in order to clean the contaminated sites. Recent researches have directed to the application of non-edible floriculture plants having the capability to erase the toxic metals from polluted environment including their aesthetic value as a good proposal for phytoremediation. The plant Tagetes erecta Linn., locally recognized as Ganda Phul (Marigold) that belongs to the family of Asteraceae can grow widely in heavy metal stress of Cd, Cr, Pb etc. The plant species can absorb and accumulate varieties of contaminated heavy metals like Pb, Cr, As, Cd, Co, Hg etc. This article includes a brief overview about the toxic impact of the Cr, Cd, and Pb on the plant. In addition, the discussion highlights recent progress on the application of phytoremediation competence of the plant, Tagetes erecta Linn. concerning with the heavy metals.

Morphocultural and molecular characterization of Athelia rolfsii associated with foot rot disease of Gomphrena globosa – A new record from India

Globe amaranth is an important floriculture plant grown for the cut flowers. The outbreak of southern blight disease was observed during 2019 in parts of the southern Karnataka. The disease incidence ranged from 9.54 to 21.13%. Disease symptoms include water-soaked lesions on the lower leaves, followed by quick wilting of the whole plant. The fungal pathogen was isolated on potato dextrose agar medium which produced white cottony mycelium with numerous reddish-brown sclerotia developed after 12 days of inoculation. A total of 12 fungal isolates were obtained from infected samples and their identity was established based on micro-morphological studies. Further, three isolates were used for molecular identification by sequencing the ITS-rDNA and tef-1α gene showed 100% sequence similarity with reference sequences. The associated fungal pathogen was identified as Athelia rolfsii. Further, pathogenicity tests were conducted on healthy plants in-vitro and in green house conditions. Typical foot rot disease symptoms were observed after 8 days of post-inoculation. The pathogen was re-isolated and its identity was confirmed. There are no reports of A. rolfsii on globe amaranth. To the best of our knowledge, this is the first report of A. rolfsii associated with southern blight disease of globe amaranth in India.

Waste managing plan for floriculture plants

Ecuador's Floriculture Industry is essential as one of the biggest flower producers exporting flowers worldwide. However, flower production generates harmful environmental waste, emitting greenhouse gases. Therefore, it is vital to implement sustainable practices. In this document, an analysis of the supply chain will be conducted to provide a cleaner production system and efficiency for the Floriculture Industry. The objective is to reduce the harmful impact on the environment, searching solutions to minimize water use and develop energy-efficient technology. As a result, we look forward to sustainable flower production, protecting the environment and natural resources.

Anthocyanin Content and Antioxidant Activity of Red Chrysanthemum (Chrysanthemum morifolium Ramat.) at Different Flower Ages

Chrysanthemum sp. is a floricultural plant of the Asteraceae family with high economic value. The anthocyanin pigment in red chrysanthemum acts as an antioxidant, the content of which can be influenced by genetic factors such as the physiological age of the flower. This study aimed to determine the effect of flower age on anthocyanin content and antioxidant activity in red chrysanthemum plants. The age of red chrysanthemum used was 115 Days After Planting (DAP) (early bloom stage), 120 DAP (half-bloom stage), 125 DAP (blooming stage), and 134 DAP (wilted flower). The anthocyanin content was analysed using spectrophotometric methods. Antioxidant activity was determined by the DPPH method and then the absorbance was measured using a UV-Vis spectrophotometer. The results showed that the highest anthocyanin content was obtained at the age of 134 DAP at 3.56 mg/g, followed by the age of 115 DAP at 2.40 mg/g, then at 125 DAP at 1.95 mg/g and the lowest at 120 DAP at 1, 69 mg/g. The highest antioxidant activity was shown in chrysanthemum flowers aged 115 DAP which had an IC50 value of 288.85 g/ml. The research shows that wilted chrysanthemum flowers still contain anthocyanins so it can be used in various industrial fields such as chrysanthemum tea and additives for soap.

Establishment of an efficient transformation method of garden stock (Matthiola incana) using a callus formation chemical inducer.

Matthiola incana is an important floricultural plant that blooms from winter to spring, and had been desired to be established a transformation system. This study successfully obtained stable transgenic plants from M. incana. We used Agrobacterium tumefaciens harboring a binary vector containing the β-glucuronidase gene (GUS) under the control of cauliflower mosaic virus 35S promoter to evaluate the transformation frequency of M. incana. We observed that cocultivation with the A. tumefaciens strain GV3101 for 5 days effectively enhanced the infection frequency, assessed through a transient GUS expression area in the seedling. Furthermore, the addition of 100 µM acetosyringone was necessary for Agrobacterium infection. However, we could not obtain transgenic plants on a shoot formation medium supplemented with 1 mg l-1 6-benzyladenine (BA). For callus formation from the leaf sections, a medium supplemented with 1-50 µM fipexide (FPX), a novel callus induction chemical, was employed. Then, the callus formation was observed after 2 weeks, and an earlier response was detected than that in the BA medium (4-6 weeks). Results also showed that cultivation in a selection medium supplemented with 12.5 µM FPX obtained hygromycin-resistant calli. Thus, this protocol achieved a 0.7% transformation frequency. Similarly, progenies from one transgenic line were observed on the basis of GUS stains on their leaves, revealing that the transgenes were also inherited stably. Hence, FPX is considered a breakthrough for establishing the transformation protocol of M. incana, and its use is proposed in recalcitrant plants.

Diversity of Floricultural Crops traded in Langsa City Aceh Indonesia

Indonesia is a tropical country that has abundant biodiversity, including floriculture plants. The purpose of this study was to determine the diversity of floricultural plants traded in Langsa City. Determination of the research location by purposive sampling. This research was conducted using survey and interview methods, namely tracing floriculture plant traders in each sub-district of Langsa City. The data obtained were analyzed qualitatively, namely grouping by type (species) and family. The diversity of floricultural plants traded in Langsa City is 234 species belonging to 61 families. In Langsa Baro sub-district there are 109 species and 39 families; Langsa Barat as many as 52 species and 19 families; Langsa Kota as many as 96 species and 37 families; Langsa Lama as many as 26 species and 12 families; Langsa Timur as many as 86 species and 30 families. The Araceae family is the largest family found in all floricultural plants traded in Langsa City, which is as many as 51 species.

Mites and insects occurring on some leafy ornamentals and floricultural plants in Howrah and Purba Medinipur districts of West Bengal, with their economic importance

The present paper deals with a total of 26 species of mites under 10 families, 17 genera and 3 orders as well as 10 species of insects under 5 families, 9 genera and 3 orders, collected from some floricultural and leafy ornamental plants in Howrah and Purba Medinipur districts of West Bengal. It may be mentioned here that these two districts are the largest producers of these plants. Among the mites, there are 14 species under 4 families and 8 genera which belong to phytophagous group, 10 species under 4 families and 7 genera belong to predatory group and remaining 2 species under as many families and genera belong to fungal feeding group. All the species of mites and insects are listed with their respective host/habitat plants, collection localities as well as their economic importance, if any. The most injurious species of mites which were observed in the field are Tetranychus urticae on Dahlia variabilis, Oligonychus biharensis on Rosa centifolia, Eutetranychus orientalis on Tabernaemontana divaricata and, Eotetranychus suginamensis on Nyctanthes arbor-tristis. Among the predatory mites, Amblyseius largoensis and Amblyseius herbicolus are important predators on phytophagous mites and the occurrence of the other predatory mites are only occasional. Among the insects, Ferrisia virgata, Aphis gossypii and Scirothrips sp. on Codiaeum variegatum, and Ferrisia virgata on Hibiscus rosa-sinensis, respectively were found abundantly.

<i>AGAMOUS</i> correlates with the semi-double flower trait in carnation

Flower type is the most valuable ornamental trait in floricultural plants, for which the ABCDE model was proposed to explain the identity of each floral organ in flowering plants. The C-class gene <italic>AGAMOUS</italic> (<italic>AG</italic>) is responsible for stamen formation and plays an essential role in the double flower phenotype. A previous study in carnation revealed that the mutation in the miR172 binding site of the A-class gene <italic>APETALA2</italic> (<italic>AP2</italic>) leads to petal accumulation. And the expression level of <italic>AG</italic> was reduced significantly in the double flowers compared with that in the single flowers. However, there was no sequence polymorphism detected between <italic>AGs</italic> isolated from the double flowers and single flowers. Here, we performed <italic>AG</italic> analysis using single and semi-double flower carnations, and detected several mutations located in the crucial position like the MADS-box domain in the <italic>AGs</italic> of semi-double flower carnations while no changes were found at the miR172 binding site of <italic>AP2</italic>. As a result, the expression levels of <italic>AGs</italic> are reduced in the semi-double flower carnation, which could be caught by the loss function of <italic>AGs</italic>. Our data proves that <italic>AGs</italic> mutations are also associated with the semi-double flower formation in carnation, complementing the lack of research about <italic>AG</italic>-mutation-associated double flower formation in carnation.

Evaluating the Growth-promoting Effects of Microbial Biostimulants on Greenhouse Floriculture Crops

Microbial biostimulants can promote ornamental plant growth during production and improve crop performance under abiotic stresses. Even though biostimulants have shown potential in many agricultural applications, the effectiveness and specificity of many products are not well understood. The objective of this study was to analyze the growth-promoting effects of microbial biostimulants during the greenhouse production of floriculture crops. We evaluated 13 biostimulant products in greenhouse-grown zinnia (Zinnia elegans ‘Magellan Ivory’) and petunia (Petunia ×hybrida ‘Carpet White’) at low fertility (one-third of the optimal fertilizer concentration). Biostimulant products 1 and 2 containing multiple species of beneficial bacteria and fungi, and product 10 containing Bacillus subtilis QST 713, were found to increase various aspects of plant growth, including the growth index, leaf chlorophyll content (SPAD index), and shoot biomass. Both flower biomass and numbers were greater in petunia treated with product 1, and leaf size increased in zinnia treated with products 1, 2, and 10. Plants treated with these effective biostimulants at low fertility had similar or better growth and quality than untreated plants grown under optimal fertility. The concentration of various nutrient elements in leaves was higher in zinnia plants treated with biostimulant products 1, 2, or 10 compared with the negative control. Some putative mechanisms for biostimulant effectiveness, the possible reasons for biostimulant ineffectiveness, and the potential for using biostimulants as a sustainable cultural strategy are discussed. This study provides useful information about microbial biostimulant effectiveness, which is important for the development and utilization of biostimulants in the greenhouse production of floriculture plants.

Mitochondrial Loci Enable Specific Quantitative Real-Time PCR Detection of the Pathogen Causing Contemporary Impatiens Downy Mildew Epidemics.

Impatiens downy mildew (IDM) disease is a primary constraint on the production of Impatiens walleriana, a popular and economically important floriculture plant. IDM is caused by the biotrophic. oomycete Plasmopara destructor that emerged as a pathogen of I. walleriana in the 2000s. To enable P. destructor detection and quantification, a hydrolysis-probe-based quantitative PCR diagnostic assay was developed based on unique orientation and order of the mitochondrial cytochrome c oxidase subunit1 (cox1) and ATP synthase subunit alpha (atp1) genes in the genus Plasmopara. Nucleotide sequences and analysis of the cox1/atp1 region distinguished P. destructor and its sister-species P. obducens, consistent with prior phylogenetic analyses using cox2 and rDNA markers. Specificity for P. destructor was incorporated into a hydrolysis probe targeting the cox1 gene and flanking primers that amplified across the cox1/atp1 intergenic region. The limit of detection was 0.5 fg/μl of P. destructor DNA (∼100 plasmid copies/μl), with amplification efficiency = 0.95. The assay was validated against a panel of target and nontarget oomycetes, which showed that the primers were specific for Plasmopara spp., while the probe was specific for P. destructor infecting both I. walleriana and I. balsamina. Testing of Impatiens tissue collected from 23 locations across 13 states indicated all samples with IDM symptoms tested positive for P. destructor. Asymptomatic plants from two locations also tested positive for P. destructor.

Influence of foliar application of glycinebetaine on Tagetes erecta L yield cultivated under salinity conditions.

Tagetes genus of Composite family consider one of the most favorite floriculture plants. Therefore, of particular interest examine the salt tolerance of this bedding and coloring agent plant. In this research, was report the role of glycinebetaine (GB) in attenuating the adverse impacts of salt stress in African marigold plant, along with their anti-oxidative capacities and biochemical attributes. The salt stressed African marigold (100 and 150 mM NaCl) was treated with GB at 200 mM, beside untreated control plants. According to the obtained results, the growth characters were negatively in salt stressed plants but a mitigate impact of GB were observed in this respect. Obviously, the morphological as well as some physiological characters were reduced with salinity treatments while GB treatment reverses these effects. Overall, the alleviate impact of GB on the negative impact of salt stress was enhanced through improving total phenolic and antioxidant enzyme activity. Further, it is concluded that GB concentration induces the activities of antioxidative enzymes which scavenged ROS increased under saline conditions.

Post-transcriptional gene silencing of the chalcone synthase gene CHS causes corolla lobe-specific whiting of Japanese gentian.

Post-transcriptional gene silencing of the chalcone synthase gene CHS specifically suppresses anthocyanin biosynthesis in corolla lobes and is responsible for the formation of a stripe type bicolor in Japanese gentian. The flower of Japanese gentian is a bell-shaped corolla composed of lobes and plicae, which is painted uniformly blue. However, the gentian cultivar 'Hakuju' shows bicolor phenotype (blue-white stripe corolla), in which anthocyanin accumulation is suppressed only in corolla lobes. Expression analysis indicated that steady-state levels of chalcone synthase (CHS) transcripts were remarkably reduced in corolla lobes compared with plicae during petal pigmentation initiation. However, no significant difference in expression levels of other flavonoid biosynthetic structural and regulatory genes was detected in its lobes and plicae. On feeding naringenin in white lobes, anthocyanin accumulation was recovered. Northern blotting probed with CHS confirmed the abundant accumulation of small RNAs in corolla lobes. Likewise, small RNA-seq analysis indicated that short reads from its lobes were predominantly mapped onto the 2nd exon region of the CHS gene, whereas those from the plicae were scarcely mapped. Subsequent infection with the gentian ovary ringspot virus (GORV), which had an RNA-silencing activity, showed the recovery of partial pigmentation in lobes. Hence, these results strongly suggested that suppressing anthocyanin accumulation in the lobes of bicolored 'Hakuju' was attributed to the specific degradation of CHS mRNA in corolla lobes, which was through post-transcriptional gene silencing (PTGS). Herein, we revealed the molecular mechanism of strip bicolor formation in Japanese gentian, and showed that PTGS of CHS was also responsible for flower color pattern in a floricultural plant other than petunia and dahlia.

ASSOCIATION OF PHYTOPLASMA AND BEGOMOVIRUS WITH SOME ORNAMENTAL PLANTS: A REVIEW

In recent years, the development of the floriculture sector has received rising attention, particularly for the benefit of small-scale agricultural enterprises producing domestic seedlings of perennial ornamental plants and for export to neighbouring countries. Plant diseases, including those associated with phytoplasma infections and plant viruses, are affected by this industry, as are other sectors of the agricultural economy.In a number of commercial cut flowers and ornamental plants, phytoplasma and plant virus infection causes diseases, causing major economic losses globally.Therefore, phytoplasma and plant virus diseases are the key constraints in the production of lucrative ornamental plants and lower their quantum and quality, gaining international importance due to unspecific symptoms, different losses and complex epidemiology around the world. These disease epidemics forced the removal of several varieties of floricultural plants such as gladiolus, lily, chrysanthemum and rose from cultivation. In various ornamental plants in botanical gardens and various floriculture farms, symptoms of general yellowing as well as plant stunting, shoot proliferation, phyllody, virescence, lower cost of flowers and reddening of leaves were observed. The prevalent mode of distribution of plant viruses is vector transmission, vegetative propagation or seed, although in some cases, viruses are transmitted by mechanical contact. Begomoviruses in economically important ornamental plants, especially in the tropical and subtropical regions, are among the most dangerous epidemic-causing pathogens, but phytoplasmas of ornamental plants have been widely distributed geographically. Information on phytoplasma and begomovirus infecting ornamental plants has been addressed in this study

Economic overview of ornamental flowers and plants in Brazil

This work aims to study the panorama of the floriculture and ornamental plants sector in Brazil, highlighting the main aspects related to area, production and distribution in the national scenario and consumption, the material used to obtain the data regarding the research carried out, was extracted from sources of research. Floriculture was an activity that was restricted only in the Southeast, more specifically in the State of São Paulo. Currently, due to the work of several institutions, this sector can be expanded to other regions of the country. Floriculture has great socioeconomic potential, generating jobs mainly for small producers. Although floriculture has great potential for expansion, it still has great difficulties due to the lack of habit of Brazilians in consuming flowers. The results showed that the domestic market generates a considerable source of income for the population in general, the ease of access of these products, makes the consumption of flowers in the country have gained more and more space, although it is very low in relation to other countries. Brazil has resulted in annual growth of 6.26% in recent years, moving around US $ 750 million per year, with a per capita consumption of US $ 4.70 and has 18,000 sales points throughout the country. The foreign market, however, has a remarkable growth, however, it needs to conquer much more space with other South American countries, corresponding to only 0.22% of the world market. Brazil exports to 40 destinations, with Holland being the largest buyer, followed by the United States. In 2011, Brazil exported US $ 20,699,521, 40.33% of which was related to ornamental plants, 48.37% for bulbs, rhizomes and similar, 3.67% fresh flowers and 7.63% of other products. It is concluded that there is a need to formulate strategies to manage in a way that the productive and commercial sectors grow in order to strengthen the market.

Progress in the genetic modification of blue flowers based on anthocyanin metabolism

As water-soluble, natural pigments, anthocyanins are responsible for the red, purple and blue colors of many flowers, which attract pollinators to spread pollen. The colors of flowers are also essential for plants to survive in the nature and become one of the most significant characteristics of ornamental plants. In the booming floriculture industry, to produce various flower colors could increase the richness of natural colors, but it is still difficult to breed flowers with coveted blue color. The diversity of flower color is mainly determined by the types and contents of anthocyanins and their derivatives. The synthesis of delphinidin pigments is the key factor for breeding blue flowers. However, there are no structural genes in many plants to biosynthesize delphinidin pigments. Blue flowers are successfully created by genetic engineering in recent years. In this paper, using common ornamental plants as examples, we review the mechanism of plant flower coloration from the aspects of the key factors affecting the synthesis of delphinidin pigment and the production strategies of blue flowers based on the regulation of anthocyanin metabolism. Different strategies of molecular breeding could provide opportunities to improve colors of other floriculture plants and to develop anthocyanin-rich economic crops, such as colored cotton with blue fibers.

Floriculture: alternate non-edible plants for phyto-remediation of heavy metal contaminated soils

Contamination of pre-urban arable land, by untreated municipal/industrial effluents derived heavy metals, is causing serious health hazards to human beings and abiotic components of the ecosystem. In this study, phytoremedial potential of four non-eatable floriculture plants, i.e. antirrhinum, pansy, calendula, and marigold, was explored by growing in heavy metal contaminated soil (collected from pre-urban area under untreated wastewater irrigation for more than 20 years) amended with bacterial inoculum and EDTA amended soils under greenhouse conditions for 75 days. Bacterial inoculation gave a maximum increase in the root (47.1%) and shoot (30.9%) biomass, while EDTA amendment gave 37.1 and 21.4%, respectively. However, EDTA application increases more metal concentrations in the root (65%) and shoot (36%) than that of bacterial inoculum, i.e. 37 and 27%, respectively. The values of bioconcentration factor (BCF) of all the plants for Cd, Cr, Ni and Pb were significantly increased by EDTA application and bacterial inoculum over control. The BCF values were either ≈1 or >1 in all the treatments in case of Cr. Ni and Pb. Contrarily, reduction in translocation factor (TF) values of all the flowering plants for all the metals were observed over control when the growth medium was treated with EDTA and bacterial inoculum.

ANALISIS PENDAPATAN USAHATANI BUNGA MELATI (Jasminum sambac) DI KABUPATEN BATANG PROVINSI JAWA TENGAH

Jasmine is a type of floriculture plant, that is white and has a very fragrant aroma. Jasmine flowers are widely used for activities related to customs as well as industrial raw materials. The use of jasmine is increasingly decreasing because many industries use the essence of jasmine because they are cheaper. The selling price of jasmine flowers tends to fluctuate because the production of jasmine is determined by the season and requests for certain festivals and ceremonies. The purpose of this study was to analyze the income of jasmine flower farmers and analyze the factors that influence the income of jasmine flower farming. The research was carried out in September 2018 in Batang District and Kandeman District, Batang Regency. The research method uses survey methods and data collection methods through interviews. The method for taking samples using Slovin techniques, determining the number of each sub-district using proportional allocation. Methods of data analysis using income analysis, multiple linear regression test and one sample t-test. The results showed that the income of jasmine flower farmers was Rp. 3,330,909 / 0.51 ha / month. The results of the analysis of one sample t-test show that the income of jasmine flower farmers is greater than the District Minimum Wage (UMK). Factors of seed costs, pesticide costs, fertilizer costs, selling prices and labor costs have an effect on simultaneously or partially on the income of jasmine flower farmers.

Regeneration response of carnation cultivars in response of silver nanoparticles under in vitro conditions

The purpose of this study was to analyze the effect of chemically synthesized silver nanoparticles (AgNPs) on in vitro regeneration of carnation cultivars cv. Noblessa, cv. Antigua and cv. Mariposa. Number of shoots/explant of cv. Noblesse and cv. Antigua significantly increased at 6 mg/L AgNPs (average 7.33 shoots per explant) when supplemented in MS media. While cv. Mariposa showed highest regeneration rate at 8 mg/L (average 10 shoots per explant). High concentration of AgNPs (12 mg/L) in the medium enhanced rooting response, number of roots/plant, and root length as compared with control. The fresh and dry weight of regenerated plants significantly (P < 0.05) increased at 6 mg/L. DPPH based free radical scavenging activity, total antioxidant activity and reducing power potential of regenerated plants varied depending upon concentration of AgNPs in the media. To find non-enzymatic antioxidants to combat oxidative damage, total phenolics and flavonoids were also determined in the regenerated plants. The study concludes that metallic nanoparticles has significant effect on in vitro growth of carnation cultivars however concentration dependent. Furthermore, nanoparticles can be effectively used for increased in vitro shoot multiplication and regeneration of floriculture plants.