The article is devoted to the variation of generation potential of the Lower-Middle Jurassic organic matter in the Karabash area (South-Western part of the West Siberian basin) depending on facies and sedimentary cyclisity. Based on geochemical and lithological core studies, specific geochemical features of organic matter established for main facies groups of Early-Middle Jurassic in the study area. The best generation potential inherent in deposits of swamps, swampy floodplains and tidal plains. These facies groups characterized by predominance of the continental organic matter and considered to gas source rocks. However, macerals composition, kinetic spectra and biomarker analysis show the presence also of marine organic matter. Some facies groups, such as flooded swamps and swampy floodplains has potential to generate hydrocarbons due to preservation of liptinites with high hydrogen index. Cyclic analysis with basics of sequence stratigraphy of the continental deposits allow tracing the correlation between generation potential and relative sea level changes.