Cyclodextrin glucanotransferase (CGTase) production was studied using loofa-immobilized Bacillus sp. DSM 2523 and starch substrate. The bacterial cells from 2- and 4-day old cultures were used in the flask studies. Loofa and chitosan were added for cell immobilization and cell flocculation, respectively, and different treatments were considered according to the timing of their addition. The cell responses were evaluated for their degree of cell immobilization and level of CGTase activity. With the use of the selected treatment, testing was carried out in an airlift reactor with a net draft tube having specific geometric properties. The cell capacity for reusability also was examined. The production of CGTase was higher in the second cycle in the reactor operated at 0.25 vvm where the enzyme activity reaching 0.20 U/ml within 2 h. In the flask experiments, CGTase activity reached 0.23 U/ml after 19 h in the second cycle.