The Caucasus, situated strategically in terms of natural geography and abundant raw materials, has been attractive to the interest of prehistoric hunter-gatherers throughout ancient times. Recent discoveries at the Neolithic site of Makhvilauri in the Ajara region, affirm this historical focus. The Makhvilauri flaked stone tool assemblage encompasses various lithic resources, of which ∼11 % (n = 59) comprise obsidian, a raw material whose closest sources are 170 km away. Using the XRF method at the Archaeometry Laboratory at the University of Missouri Reactor Research (MURR) to elementally characterise 23 of the obsidian artifacts (∼39 % of the total) we can demonstrate that the Makhvilauri assemblage was made from at least five geochemically distinct sources. This analysis not only sheds light on the mobility patterns of ancient communities but also underscores the extensive contacts established during the Neolithic period (6th millennium BC).