Specific in-flight infection prevention and control (IPC) training was deemed an essential strategy for ensuring the implementation of appropriate measures to protect the health of passengers and crew sustainably during COVID-19 and the post-pandemic period. This study aims to identify cabin crew’s IPC duties and investigate the current status of the in-flight IPC training for cabin crew in China. The snowballing technique was employed with purposeful sampling. A total of 15 participants, including cabin crew of various positions, inspectors, and trainers from eight airlines in China were invited to attend the in-depth semi-structured interview between September and November of 2022. Cabin crew IPC duties can be classified into four phases based on a thematic analysis: before flight, passenger boarding, during flight, and after flight. The findings indicated airlines in China required their cabin crew to carry out strict IPC measures onboard throughout all stages of flight, including in-flight IPC training before flight, being aware of COVID symptoms and monitoring passengers’ conditions during boarding, the standard operating procedure (SOP) of handling suspicious carriers during flight, and so on. Meanwhile, six major themes concerning cabin crew’s in-flight IPC training were extracted: COVID-19 information, IPC Supplies and usage, IPC measures onboard, communication and explanation, information collection and report up, attitude and perceptions toward COVID-19, and IPC measures. Cabin crew were regularly trained on in-flight IPC through online learning, with face-to-face training used as reinforcement. Although training for knowledge and technical skills was deemed comprehensive, non-technical skills such as communication and explanation were viewed as a weak link. Moreover, airlines should make greater efforts to help cabin crew develop a positive attitude towards COVID-19 and overcome their internal fears. This study pointed out the shortcomings of the in-flight IPC training for cabin crew in China and can help the airlines improve the training program so that cabin crew can perform their duties correctly. Furthermore, the result can inform the development of future cabin crew training programs for the post-pandemic period.