The main objective of the current research is to establish experimental data for minimum flexural reinforcement, ρmin, of high strength concrete (HSC) rectangular beams. Nine full-scale singly reinforced beams with flexural reinforcement ratios varying from 50% to 100% of the minimum limit specified by the ACI 363R-35were tested in flexure. Concrete compressive strengths of 52, 73 and 96.5MPa were used. The test results including crack patterns, deflections and strains in the tensile flexural steel bars show that a 25% reduction of the ACI 363R-35 limit for the ρmin would result in a satisfactory flexural beam behavior with a reserve flexural parameter (Py,/Pcr)⩾1.29 and a displacement ductility index λΔ>5 for all concrete grades which may lead to good savings in the amount of the flexural reinforcement. Also, it was noted that the displacement ductility index λΔ increased as the concrete compressive strength increased for the same ratio (ρ/ρmin) up to 75MPa and then decreases as fcu increases. For the same concrete compressive strength with low values of flexural reinforcement ratio, ρ, the displacement ductility index λΔ increased as ρ increased. The experimental results of this study were compared with the limits specified by available codes and researches.