Abstract Photonic spin Hall effect (PSHE), as a novel physical effect in light–matter interaction, provides an effective metrological method for characterizing the tiny variation in refractive index (RI). In this work, we propose a multi-functional PSHE sensor based on VO2, a material that can reveal the phase transition behavior. By applying thermal control, the mutual transformation into different phase states of VO2 can be realized, which contributes to the flexible switching between multiple RI sensing tasks. When VO2 is insulating, the ultrasensitive detection of glucose concentrations in human blood is achieved. When VO2 is in a mixed phase, the structure can be designed to distinguish between the normal cells and cancer cells through no-label and real-time monitoring. When VO2 is metallic, the proposed PSHE sensor can act as an RI indicator for gas analytes. Compared with other multi-functional sensing devices with the complex structures, our design consists of only one analyte and two VO2 layers, which is very simple and elegant. Therefore, the proposed VO2-based PSHE sensor has outstanding advantages such as small size, high sensitivity, no-label, and real-time detection, providing a new approach for investigating tunable multi-functional sensors.