Collagen is the most abundant structural protein in animals, being the main component of the skin, and can be researched and utilized by the cosmetics, pharmaceutical, food, and packaging industries. The present study investigates the extraction and quantification of collagen from three different animal sources: beef mocotó, chicken feet, and tambaqui swim bladder. Using the method of extraction in soluble acid solution (acetic acid) and precipitation in sodium chloride saline solution, collagen yields and their physicochemical properties were determined. The results revealed that the tambaqui swim bladder, especially the outer layer, presented the highest yield (39.97%), followed by chicken feet (3.49%) and bovine mocotó (1.79%). This study highlights the viability of these sources for the production of high-quality collagen, with potential applications in the cosmetics, pharmaceutical, and biomaterials industries, promoting a sustainable and economical approach to the use of animal by-products.