PURPOSE: The purpose of this study was to assess associations of predominately sedentary behaviors and physical activity status with physical fitness among college students. METHODS: 122 students (53 males and 69 females) currently enrolled in a university level general education wellness class participated in the study. Participants completed a physical fitness battery consisting of cardiorespiratory fitness (CRF), muscular endurance (ME), body composition (BC) and flexibility (FX) measurements. In addition, they completed a survey to assess time spent in predominantly sedentary behaviors including computer (CT) and internet (IT) use, television watching (TV), texting (TX) and playing video games (VG). Self-reported physical activity levels were also assessed to determine if participants were inactive, insufficiently active or active according to the aerobic component of the current physical activity recommendation. Tests of gender differences in each of the study variables were conducted using Mann-Whitney Tests. Linear regression analysis using ranked transformed variables were used to assess relationships between physical fitness and time spent in sedentary behaviors with gender included as a covariate to control gender effects. RESULTS: Statistically significant gender differences were observed for each of the physical fitness measures (males > CRF, females > BC, FX, each p <.001) with the exception of ME (p =.079). In addition, males tended to be less active than females in this study (p =.045). Gender differences were also observed for time spent watching TV (males > females, p =.032), texting (females > males, p <.001), and video game use (males > females p <.001). After controlling for gender effects, statistically significant inverse associations were found between ME and IT (beta = -.22, p =.024); between FX and CT (beta = -.20, p =.049), and between FX and IT (beta = -.23, p =.025). Finally, an inverse statistically significant association of overall activity level with CT (beta = -.24, p =.011) and IT (beta = -.25, p =.007) was observed. CONCLUSION: The relatively few relationships noted between sedentary behaviors, physical activity status and physical fitness variables may be due to the homogenous lifestyles of college students in this sample regarding sedentary behaviors.