Fire protective clothing worn by the emergency responders may be exposed to intensive heat condition time and time again during a firefighting work. In this research, the level of thermal protection retained by the fire protective clothing after repeated exposures to flash fire was investigated from bench-scale test to full-scale test. A thermal protective performance tester and an instrumented manikin with a transverse motion system device which was capable of simulating the action of running across the flame were used for the exposure test. Physical properties (mass, thickness, thermal shrinkage, tear strength) and thermal protective property of the test specimens were examined after each exposure. The results showed that repeated heat exposures resulted in continuous decrease of mechanical performance of the fabrics. The thermal protective performance of fabrics with good thermal dimensional stability such as polybenzimidazole/Kevlar and flame resistant cotton decreased after exposure. For the fabrics with severe thermal shrinkage such as Nomex IIIA and polysulfonamide, the thermal protective performance was improved due to the increase of fabric thickness induced by thermal shrinkage. However, this positive effect of thermal shrinkage diminished in the manikin test as it decreased the air gap size between the garment and flame manikin. The thermal protective property of Nomex IIIA garment exhibited continuous decrease after repeated exposures. This study was expected to provide new sights for the performance evaluation and application of fire protective clothing.