Flame Photometric Detector Research Articles

Determination of Aromatic Arsines in Environmental Solids by Direct Thermal Desorption Gas Chromatography

ABSTRACTTo quantify aromatic arsines in the environment, such as World War I era chemical warfare agents and degradation products of arsenicals used in agriculture, a sensitive, selective, and direct method is needed. We describe the development and optimization of a method for the measurement of trace levels of triphenylarsine used as a model aromatic arsenic compound. Triphenylarsine was determined at low µg/g levels in sand, soil, and lake sediment by thermal desorption before gas–liquid chromatography (GC) with mass spectrometric and pulsed flame photometric detection. The dithiol derivative of phenylarsonic acid was used as an internal standard, thereby significantly improving the precision of the method. The desorption conditions were studied and found to be optimal at 350°C for 15 min. Significant improvement in precision was realized by preparing the solid samples as slurries in acetone and by inserting a small (∼100 mg) quartz wool plug into the sample vial. The method was applied to determine triphenylarsine in authentic soil and sediment samples that had been fortified with triphenylarsine and aged for at least 15 days. Recoveries for soil samples ranged from 84.3 ± 2.3 to 87.7 ± 1.3%, while lower recoveries were obtained for sediment samples (75.1 ± 3.0%). The detection limit for triphenylarsine in soil was 3.14 ng with a precision of 7.10% (n = 4). Using these optimized conditions, the performance of the direct thermal desorption GC method for sample introduction was greatly improved compared to methods that have been reported in the literature.

Pesticide residue analysis in Clarias gariepinus sold in Wukari market Taraba State, Nigeria

This study reports the concentrations of the pesticide residues in Clarias gariepinus samples sold within Wukari Market using gas chromatography equipped with electron capture and flame photometric detectors. Among the pesticides analyzed in this study: α-BHC, β-BHC, chlorothalonil, heptachlor, aldrin, o,p-DDE, endosulfan I and II, P,P-DDE, PCB 153, PF-38, P,P-DDT were below LOD while lindane (0.006±0.0015) dieldrin (0.007±0.0012 µg/g), a-cypermethrin (0.072±0.0036 µg/g), b-cypermethrin (0.039±0.0012 µg/g), z-cypermethrin (0.031±0.0014 µg/g) were detected but at concentrations below the maximum regulatory limit (MRL) set by World Health Organization (WHO). However, adequate measures should be taken to preserve the aquatic life in the study area.

Determination of Cisplatin and Carboplatin Anticancer Drugs by Non-suppressed Ion Chromatography with an Inductively Coupled Plasma Atomic Emission Detector

ABSTRACTTwo non-suppressed ion chromatographic (IC) methods, one with an anion and one with a cation separation column, were investigated for first time to determine cisplatin and carboplatin anticancer drugs using inductively coupled plasma–atomic emission spectrometry as a detector. The Shodex IC YK-421 (4.6 × 125 mm2) column was considered as the preferred separation column. The mobile phase in this case consisted of tartaric acid and boric acid. The flow rate was 1 mL/min and the injection volume was 20 μL. Separation was carried out in about 2 min with column temperature at 30°C after optimization. With cationic separation, the cisplatin elutes first, as opposed to the anionic one, where it elutes second. In addition, with both columns, a second peak for cisplatin appears which is attributed to a hydrolysis product of the drug. For the cation chromatographic method, the repeatability ranged from 3.1 to 5.9%, whereas the inter-day precision was 13.3 and 16.3% for cisplatin and carboplatin, respectively. The detection limits were 0.1 mg/ L Pt for both compounds. The proposed method was applied to the analysis of human urine with satisfactory recoveries indicating that there are no matrix effects.

Application of a magnetic graphene nanocomposite for organophosphorus pesticide extraction in environmental water samples

A novel magnetic nanocomposite was prepared and applied for the extraction of organophosphorus pesticide residue (malathion, chlorpyrifos, isocarbophos, fenamiphos, profenofos) in environmental water by magnetic solid phase extraction. The magnetic nanocomposite was synthesized by coating Fe3O4 with silicon dioxide and then bonding with graphene oxide under the catalytic action of hydrazine hydrate. A residue analysis method for the determination of organophosphorus pesticides in environmental water was established via magnetic solid phase extraction with the nanocomposite as the sorbent, followed by gas chromatography with flame photometric detection (GC-FPD). Various experimental parameters affect extraction efficiency and must be optimized, such as the sorbent amount, the type and volume of desorption solvent, desorption time, desorption step, salt concentration and pH. Under the optimum conditions, a linear response was achieved for malathion and chlorpyrifos in the concentration range of 0.05 mg L−1–5 mg L−1. Other organophosphorus pesticides were quantified in a concentration range of 0.1 mg L−1–5 mg L−1, and the coefficient of correlations were between 0.996 and 1 for this method. The method was used to determine organophosphorus pesticides in river water and groundwater. The average recoveries ranged from 90.2% to 102.9%, with low relative standard deviations (RSD), and the limits of detection (LOD) were between 0.016 mg L−1 to 0.033 mg L−1 at a signal to noise ratio of 3. The method was efficient for the residue analysis of organophosphorus pesticides in water with easy operation, high sensitivity, and good precision.

Determination of organotin compounds (OTC) at low levels in seawater by solid-phase extraction (SPE) and gas chromatography-pulsed flame photometric detection (GC-PFPD)

ABSTRACTIn this work, a simple, sensitive and affordable analytical method for the simultaneous determination of nine organotin compounds (butyltins, phenyltins and methyltins) in seawater using solid-phase extraction (SPE) and gas chromatography-pulsed flame photometric detection was developed and validated. The performance of three different SPE cartridges (Envi C18, Oasis HLB and Oasis MCX) and three elution solvents of different polarity (hexane, methanol and acetonitrile) was evaluated. The extraction parameters, such as solvent volume, presence of complexing and ion-pairing reagents, sample volume and pH and breakthrough volume, were also investigated. Tributyltin, as the organotin compound of special interest, was efficiently extracted using any of the cartridges and solvents tested. However, the simultaneous extraction of all nine organotin compounds was the most efficient using reversed-phase Envi C18 cartridge and 0.1% (w/v) tropolone in methanol as eluent. The optimised method resulted in good recovery, precision and linearity for all compounds, particularly for tri- and disubstituted species. Method detection limits ranged from 0.22 to 1.27 ng(Sn) L−1 for butyltins, 0.37 to 4.91 ng(Sn) L−1 for phenyltins and 0.45 to 1.16 ng(Sn) L−1 for methyltins. The accuracy of butyltins determination was further verified by the comparison with purchased derivatised standards. The developed method was successfully applied to the environmental samples.

Dissipation of Chlorpyriphos and Profenophos in Cabbage (Brassica oleracea var. capitata L.)

The persistence of organophosphorus insecticides chlorpyriphos and profenophos @ 300 and 500 g a.i. ha−1 in cabbage following two foliar applications at 15 d interval during the head formation stage, is reported. Samples collected at 0 (2h), 1, 3, 5, 7, 10 and 15 d after the last (second) spray were extracted by QuEChERS method and residues estimated by gas liquid chromatography equipped with flame photometric detector (FPD). The mean initial deposits of chlorpyriphos and profenophos were 1.52 and 1.02 μg g−1 respectively which dissipated with time and reached below the quantitation level of 0.05 μg g−1 within 15 d with half-life values of 1.91 and 2.01 d, respectively. The Limit of detection (LOD) and limit of quantitation (LOQ) of the two insecticides in cabbage were 0.01 and 0.05 μg g−1, respectively.

Identification of Soil Properties and Organophosphate Residues From Agricultural Land in Wanasari Sub-District, Brebes, Indonesia

Organophosphates have been used to eradicate pests and prevent losses from harvest failures caused by pest attack. It is undeniable that the organophosphate persist in soil. This study aims to identify the organophosphate residue and soil properties include pH, soil texture, and permeability. The soil samples were taken from cropland in 10 villages, Wanasari sub-district, Brebes, Indonesia. Organophosphate residue determined by gas chromatography using Flame Photometric Detector. Soil texture was determined by soil texture triangle from NRCS USDA, and the permeability value was determined by falling head method. The mean value of chlorpyrifos, profenofos, diazinon were 0.0078; 0.0388; 0.2271 mg/l respectively. The soil texture varies from clay, silt clay, loam, silt loam, and silt clay loam with permeability value at 10-7 with the soil pH value between 6.4 – 8.1. The results showed that organophosphate residues found in the soil and its potential affect the soil fertility decline. We recommend to conduct routine soil quality analysis to prevent soil damage in the agricultural environment.

Headspace solid-phase microextraction and gas chromatographic analysis of low-molecular-weight sulfur volatiles with pulsed flame photometric detection and quantification by a stable isotope dilution assay.

Low-molecular-weight volatile sulfur compounds such as thiols, sulfides, disulfides as well as thioacetates cause a sulfidic off-flavor in wines even at low concentration levels. The proposed analytical method for quantification of these compounds in wine is based on headspace solid-phase microextraction, followed by gas chromatographic analysis with sulfur-specific detection using a pulsed flame photometric detector. Robust quantification was achieved via a stable isotope dilution assay using commercial and synthesized deuterated isotopic standards. The necessary chromatographic separation of analytes and isotopic standards benefits from the inverse isotope effect realized on an apolar polydimethylsiloxane stationary phase of increased film thickness. Interferences with sulfur-specific detection in wine caused by sulfur dioxide were minimized by addition of propanal. The method provides adequate validation data, with good repeatability and limits of detection and quantification. It suits the requirements of wine quality management, allowing the control of oenological treatments to counteract an eventual formation of excessively high concentration of such malodorous compounds.

Determination of propineb and its metabolites propylenethiourea and propylenediamine in banana and soil using gas chromatography with flame photometric detection and LC–MS/MS analysis

ABSTRACTA sensitive and specific method for the determination of propineb and its metabolites, propylenethiourea (PTU) and propylenediamine (PDA), using gas chromatography with flame photometric detection (GC-FPD) and LC–MS/MS was developed and validated. Propineb and its metabolite residue dynamics in supervised field trials under Good Agricultural Practice (GAP) conditions in banana and soil were studied. Recovery of propineb (as CS2), PDA and PTU ranged from 75.3 to 115.4% with RSD (n = 5) of 1.3–11.1%. The limit of quantification (LOQ) of CS2, PDA and PTU ranged from 0.005 to 0.01 mg kg−1, and the limit of detection (LOD) ranged from 0.0015 to 0.0033 mg kg−1. Dissipation experiments showed that the half-life of propineb in banana and soil ranged from 4.4 to 13.3 days. PTU was found in banana with a half-life of 31.5–69.3 days, while levels of PDA were less than 0.01 mg kg−1 in banana and soil. It has been suggested that PTU is the major metabolite of propineb in banana. The method was demonstrated to be reliable and sensitive for the routine monitoring of propineb and its metabolites in banana and soil. It also serves as a reference for the detection and monitoring of dithiocarbamates (DTCs) residues and the evaluation of their metabolic pathway.

Multi-residue levels of organophosphorus pesticides in cocoa beans from some cocoa farms in Ekiti state, Nigeria

This study investigated the level and occurrence of 13 organophosphorus pesticides (OPPs) residues in cocoa beans from some cocoa farms in Ekiti state, Nigeria. The pesticides in the cocoa beans were extracted and cleaned up on silica gel adsorbent. A gas chromatograph coupled with flame photometric detector (FPD) was used to quantify the pesticides. The reliability of the analytical method was examined by spiking the beans with known concentration of the pesticides and analysed following similar procedure of extraction and clean-up. The percentage of pesticides recovered ranged from 88% to 92%. Dichlorvos, mevinfos, diazinon, chlorpyrifos, dimethoate and parathion were detected with varying concentration in the bean samples whilst phosphamidon, pirimiphos-methyl, fenthion, bromophos, isofenfos, diclofenthion and ethion showed no detectable level. The concentration of OPPs ranged from ND - 58μg/kg and ND - 85μg/kg for the dry and wet seasons respectively. Analysis of variance (ANOVA) showed no significant variation (p ˃ 0.05) in the levels of all detectable pesticides among the sampled farms whilst dichlorvos, diazinon, dimethoate and chlorpyrifos showed significant difference between both seasons. Residual levels of these detectable pesticides were below the maximum residue limits (MRLs) set by the European Union in food. Hence, the cocoa beans from the study areas are considered safe for consumption.Bangladesh J. Sci. Ind. Res. 52(4), 281-288, 2017

Characterization of the key aroma compounds in mulberry fruits by application of gas chromatography–olfactometry (GC-O), odor activity value (OAV), gas chromatography-mass spectrometry (GC–MS) and flame photometric detection (FPD)

The volatile compounds of mulberries obtained from three cultivars (M. nigra, Y1, M. Macroura, Y2 and M. Alba, Y3) were investigated by gas chromatography–olfactometry (GC-O), odor activity value (OAV), gas chromatography-mass spectrometry (GC–MS) and flame photometric detection (FPD). The results showed that 41, 37 and 41 compounds were detected in Y1, Y2 and Y3 samples, respectively. In addition, 12, 11, and 12 sulfur compounds were identified in three samples by FPD, respectively. Quantitative analysis showed 2-(methylthio)ethanol, methionol, dimethyl sulfide, methional and 3-ethylthiophene were present in relatively high amounts in each of the three samples. Furthermore, benzaldehyde, ethyl butanoate, (E)-2-nonenal, 1-hexanol, hexanal, methional, 3-mercaptohexyl acetate and 3-mercaptohexanol were present with much higher OAVs than other compounds. Finally, four compounds (pentanal, ethyl acetate, acetic acid, methionol) were selected to investigate possible effects on six descriptors, which indicated that the volatile compounds at sub-threshold concentrations are likely to contribute to overall aroma.

Human health risk assessment of organophosphorus pesticides in maize (Zea mays L.) from Yushu, Northeast China

ABSTRACTIn order to investigate organophosphorus pesticides (OPs) concentrations in maize and estimate the health risk to consumers, a total of 55 samples were collected from Yushu, one of the most important maize production areas. The concentrations of the eleven detected OPs in maize were analyzed by gas chromatography coupled with flame photometric detector (GC-FPD). The results showed that OPs concentrations of all maize were not higher than maximum residue limit (MRL), 67.3% of samples below MRL and only in 32.7% of samples was not found OPs. The mean concentrations obtained for the eleven OPs in μg kg-1 were as follows: omethoate (0.8), quinalphos (0.8), phorate (0.7), dimethoate (0.7), parathion-methyl (0.6), isocarbophos (0.6), diazinon (0.5), fenitrothion (0.5), and malathion (0.5), with parathion (0.5) and fenthion (0.3). Phorate (16.4%), dimethoate (16.4%) and quinalphos (16.4%) had the highest frequency in the eleven OPs. 29.1% samples contained two or more kinds of OPs, while 38.2% samples detected one kind of OPs. The target hazard quotients (THQ) values were all less than one and the total acute hazard index (aHI) values for adults and children were 0.051 and 0.121, respectively indicating that consumer may not pose significant chronic and acute health risk through maize.

Rapid gas chromatography with flame photometric detection of multiple organophosphorus pesticides in Salvia miltiorrhizae after ultrasonication assisted one-step extraction.

A simple and rapid gas chromatography-flame photometric detection (GC-FPD) method was developed for the determination of 12 organophosphorus pesticides (OPPs) in Salvia miltiorrhizae by using ultrasonication assisted one-step extraction (USAE) without any clean-up steps. Some crucial parameters such as type of extraction solvent were optimized to improve the method performance for trace analysis. Any clean-up steps were negligent as no interferences were detected in the GC-FPD chromatograms for sensitive detection. Under the optimized conditions, limits of detection (LODs) and quantitation (LOQs) for all pesticides were in the range of 0.001-0.002mg/kg and 0.002-0.01mg/kg and 0.002-0.01mg/kg, respectively, which were all below the regulatory maximum residue limits suggested. RSDs for method precision (intra- and inter-day variations) were lower than 6.8% in approval with international regulations. Average recovery rates for all pesticides at three fortification levels (0.5, 1.0 and 5.0mg/kg) were in the range of 71.2-101.0% with relative standard deviations (RSDs) <13%. The developed method was evaluated for its feasibility in the simultaneous pre-concentration and determination of 12 OPPs in 32 batches of real S. miltiorrhizae samples. Only one pesticide (dimethoate) out of the 12 targets was simultaneously detected in four samples at concentrations of 0.016-0.02mg/kg. Dichlorvos and omethoate were found in the same sample from Sichuan province at 0.004 and 0.027mg/kg, respectively. Malathion and monocrotophos were determined in the other two samples at 0.014 and 0.028mg/kg, respectively. All the positive samples were confirmed by LC-MS/MS. The simple, reliable and rapid USAE-GC-FPD method with many advantages over traditional techniques would be preferred for trace analysis of multiple pesticides in more complex matrices.

New Study Concerning the Biodegradation of Dimethoate, Chlorpyrifos and Chlorfenvinphos in some Medicinal Plants

Herbal drugs are widely used in pharmaceutical industry due to their beneficial effects on human health. The current research was carried out to evaluate the organic pollutant degradation in the flower part of the plant after a foliar application of pesticides. Also, based on the fact that selected pesticides are highly toxic to bees, it is important to monitor the removal rate of the pollutant. Under laboratory conditions, the marigold and French flowers (Calendula Officinalis and Tagetes Patula) were spiked with a mix of organophosphorus insecticide, dimethoate, chlorpyrifos and chlorfenvinphos, where the first two substances are still used on Romanian territory. The samples were extracted at different time moments over 300 min after sprayed treatment in order to evaluate the concentration in flower. The analytes were quantified by using gas chromatography with flame photometric detection (GC-FPD) technique. The developed method ensures the quality and comparability of analytical results, with a good sensitivity in determining the analytes over the concentration range 5-250 �g/L.

Health risks due to consumption of pesticides in ready-to-eat vegetables (salads) in Kumasi, Ghana

BackgroundPesticide residue levels were determined in ready-to-eat vegetables collected from 16 sites along the food chain; which is, farms, markets, cafeterias and street food vending sites in Kumasi, Ghana. The aim of the study was to determine the concentrations of pesticides residues in two ready-to-eat vegetables and assess the health risks due to consumption of these contaminated vegetables.MethodsPesticide residues in ready-to-eat vegetables or salads were extracted by means of the QuEChERS method. Synthetic pyrethroid and organophosphorus pesticides residues in samples were determined using Gas Chromatography with Electron Capture Detector and Pulsed Flame Photometric Detector respectively. Consumption data of ready-to-eat vegetables were obtained from a questionnaire-based dietary survey in the study area. The hazard index and the relative potency factor (RPFs) approaches were used to assess the health risk from chronic cumulative dietary exposure to pesticides.ResultsThere were six synthetic pyrethroid residues detected in the ready-to-eat samples at varying concentrations. They were bifenthrin, permethrin, cypermethrin, deltamethrin, lambda-cyhalothrin and fenvalerate. Also, two organophosphates were detected in the samples; which were chlorpyrifos and diazinon. Lambda-cyhalothrin residues was present in all the samples in the study, with the mean concentration of 4.5 × 10−2 mgkg−1. The mean concentration of diazinon in all the samples (0.040 mgkg−1) exceeded the EU MRLs (0.01 mgkg−1), chlopyrifos exceeded its MRL in one sample from the street food vending site and cafeteria each. Deltamethrin, Fenvalerate and Permethrin exceeded their respective MRL in samples from Asafo (Street food vending sites) and Adum (Cafeteria) and KNUST (Farm) respectively. However, the health index of all pesticides residues detected were below the permissible limit. The cumulative intake from the RPF approach for the pesticides were relatively lower than the ADI of the index chemicals.ConclusionThe concentrations of chlorpyrifos, deltamethrin, fenvalerate, diazinon and permethrin exceeded their respective EU MRLs in some ready-to-eat vegetable samples in the study. The health index and comparison of cumulative intake from RPF with ADI of the index chemicals suggest that pesticide residues in ready-to-eat vegetables could not be considered a major public health problem.

Group-Type Analysis of Hydrocarbons and Sulfur Compounds in Thermally Stressed Merox Jet Fuel Samples

Methods to investigate the response of hydrocarbons and sulfur compounds to different extents resulting from thermal oxidation of jet fuels refined via the mercaptan oxidation (Merox) process have been developed. Relative comparison of hydrocarbon contents including n-paraffins, isoparaffins, olefins, naphthenes, and aromatics (PIONA) was performed by using comprehensive two-dimensional gas chromatography hyphenated with a quadrupole-accurate mass time-of-flight mass spectrometer (GC × GC–Q-TOFMS). A simple approach for quantification of sulfur compound groups was then developed using both GC × GC and GC hyphenated with flame photometric detection (GC × GC–FPD; GC–FPD). The approach largely separated the sulfur compounds into two distinct regions comprising of lower polarity S1 (thiophenes, thiophenols, thiols, disulfides, sulfides, and alkyl thianaphthenes) and higher polarity S2 (sulfones and phenylsulfides) groups. The reliability of this approach was evaluated using higher resolution GC × GC–FPD, show...

Determination of tributyl phosphate in the air of workplace by gas chromatography

Objective: To establish a practical method forsampling and detectingtributyl phosphate intheworkplace. Methods: The samples were extracted by glass fiber membrane, eluted with ether, separated by gas chromatography, and detected by flame photometric detector. Results: There were good linear relationship in the minimum detection concentration was 7.2-720.0 μg/ml, and the correlation coefficient was 0.999 92. The detection limit was 0.86 μg/ml, and the minimum detection concentration was 0.14 mg/m(3) (sample volume was 30 L) . Recovery rates were 99.8%-100.2%. The with-in relative standard deviations were 4.0%-5.4% and the between relative standard deviations were 2.0%-5.5%, and average samplingefficiency was about 99.1%-100.0%. Conclusion: This method conforms with the requirements of "Standardization of Methods for Determination of Toxic Substance in Workplace" . Tributyl phosphate in air could be determined accurately using this method.

Continuous and Inexpensive Monitoring of Nonpurgeable Organic Carbon by Coupling High-Efficiency Photo-oxidation Vapor Generation with Miniaturized Point-Discharge Optical Emission Spectrometry.

Currently, no applicable analyzers are available to accomplish online continuous monitoring of organic pollution, which is one of the most important factors contributing to water shortages around the world, particularly in developing countries. In this work, a sensitive, miniaturized, inexpensive, and online nonpurgeable organic carbon (NPOC) analysis system was developed for continuous monitoring of such organic pollution. This system consists of a specially designed high-efficiency UV photo-oxidation vapor generation (HE-POVG) reactor and a miniaturized, low-power (7 W) point-discharge microplasma optical emission spectrometer (PD-OES). Organics present in sample or standard solutions are pumped to the HE-POVG and efficiently converted into CO2, which is separated and further transported to the PD-OES for NPOC analysis via highly sensitive detection of carbon atomic emission at 193.0 nm. Under optimal conditions, a limit of detection of 0.05 mg·L-1 (as C) is obtained, with precision better than 5.0% (relative standard deviation) at 5 mg·L-1. This system overcomes many shortcomings associated with conventional chemical oxygen demand or total organic carbon analyzers such as long analysis time, use of expensive and toxic chemicals, production of secondary toxic waste, requirement of large, power consuming and expensive instrumentation and difficulties implementing continuous online monitoring. The system was successfully applied to sensitive and accurate determination of NPOC in various water samples and for continuous monitoring of such organic pollution in tap water.

Ionogel-Based Ionic Liquid Coating for Solid-Phase Microextraction of Organophosphorus Pesticides from Wine and Juice Samples

In this work, a novel fiber coating was prepared by using a hybrid material of silica-based ionogels and ionic liquid 1-hexadecyl-3-methylimidazolium bis(trifuluoromethylsulfonyl)imide ([C16MIM][NTf2]). Instead of chemical bonding to sol-gel matrix, high loadings of [C16MIM][NTf2] was confined into the network of silica-based ionogels and thus, the extraction efficiency was significantly improved. The prepared fiber was used for solid-phase microextraction (SPME) of four organophosphorus pesticides (OPPs) from wine and juice samples prior to gas chromatography coupled with flame photometric detection. Under the optimized conditions, the proposed method results in good linearity (R 2 > 0.9991), with low method detection limits (MDLs, S/N = 3) and method quantification limits (MQLs, S/N = 10) in the range of 0.57–4.13 and 1.89–13.76 ng L−1, respectively. The precisions of the method were assessed with repeatability and reproducibility of RSD% ˂28 and ˂23%, respectively. The spiked recoveries of OPPs from wine and juice samples were in the range of 54 ± 5–118 ± 20 and 56 ± 14–109±12%, at two different concentration levels. The expanded uncertainties at 1 μg L−1 were below 20% for target OPPs in all selected samples.

Up to 21 Different Sulfur‐Heterocyclic Fatty Acids in Rapeseed and Mustard Oil

AbstractThree sulfur‐heterocyclic fatty acids (SHFA) had been tentatively identified in rapeseed oil in the late 1980s. In this study we aimed to enrich and verify the presence of potential SHFA in one sample of native rapeseed oil, refined rapeseed oil and mustard seed oil. Fifty‐gram samples of the three oils were individually saponified and converted into methyl esters. The resulting samples were hydrogenated and subjected three times to urea complexation. The resulting extracts of native rapeseed oil and mustard oil contained 21 different SHFA with 18, 20, 22 or 24 carbons. The refined rapeseed oil contained only nine C18‐SHFA. Structure investigation of the SHFA was performed by gas chromatography with mass spectrometry (GC/MS) using methyl esters and also 3‐pyridylcarbinol esters. A direct screening of non‐enriched samples by GC/MS in the selected ion monitoring mode and by GC with flame photometric detector (sulfur‐selective) verified that the SHFA were native compounds of the oils and no artefacts of the sample preparation. Similar abundances of the four isomer groups of SHFA with monoenoic fatty acids of the same carbon number in these and five further rapeseed and mustard samples indicated that these could be the precursors of the SHFA.