Fishery Waste Research Articles

Rearing fly larvae on various substrates: nutrient composition of larvae and frass.

Management solutions for waste in southern Thailand, such as fly larvae, are tested in a laboratory using different substrates and wastes from the Thai agricultural sector. The nutritional content of the immature stages of Musca domestica Linnaeus (Diptera: Muscidae) and Hermetia illucens (Linnaeus) (Diptera: Stratiomyidae) larvae, especially their protein and fat contents, makes them a potential animal feed. Laboratory strains of M. domestica and H. illucens were reared on selected substrates to examine how diet influenced their performance and nutritional composition. Waste from aquaculture, vegetables, and fruits in southern Thailand was examined for larval diet and showed promise as a rearing substrate for these insects. Musca domestica larvae reared on fishery waste achieved the highest larval weight and fat content, whereas H. illucens larvae reared on an aquaculture waste diet had the highest protein content. These findings imply aquaculture waste could be the best choice for large-scale fly larvae production, particularly as a protein feed additive.

To Study the Need of Systemic Formwork for Waste Water Treatment at Fish Processing Plant' (A Review)

Waste water from angle preparation enterprises originates from several sources, including emptying angles, dressing, liquefying water from ice dissolution, washing equipment and utensils, treating with additional substances, and cleaning and disinfecting offices and spaces. Water is used not only to clean angles but also to wash blood, sludge, and offal from the surfaces of various handling equipment, utensils, floors, and sump pumps. The majority of computerized handling supplies come with a water shower framework that may clean hardware and remove offal. The emergence of these forms in tall water use also leads to the mixing of blood and offal in the wash water. Fish handling waste water is full of significant pollutants that are colloidal, soluble, and particulate. Features of waste water produced depend on the type of angle used for preparation, the preparation method, and the final object placed. When handling operations such as minor (angle washing or cleaning), mild (angle filet preparation), and severe (surimi planning), the degree of defilement can change. Fish waste water must have exceptionally high levels of nitrogen, fat, oil, and oil (Mist), as well as biochemical oxygen demand (BOD). In order to reduce shameful waste transfer in the environment, this extended show introduces angle waste management processors to the concepts of fishery waste categorization and the many types of treatment used. This topic includes many aspects of angle waste, separate management of fluid angle waste and strong waste using chemicals, Indore composting technique, and suitable transfer.

Improving the performance of glucose oxidase biofuel cell by methyl red and chitosan composite electrodes

This research aims to improve the output power of self-pumping glucose enzymatic biofuel cell (EBFC) and modifying the anode. Adding a fixed ratio of methyl red-chitosan (MR-CS) can effectively improve the EBFC efficiency and stability. In addition, chitosan can be obtained from discarded crustacean fishery waste objects such as shrimp and oysters, are also significant to the use of environmentally friendly materials. The catalyst was immobilized on pyrenecarboxaldehyde (PCA), polyethyleneimine (PEI) and multi-wall carbon nanotubes (MWCNT) and combined with glucose oxidase (GOx). Finally, the [PCA/GOx]/PEI/Nafion solution/MWCNT/[MR-CS] catalyst was immobilized on the carbon cloth. Experimental analysis was progressed under the preparation of enzyme-supported electrode to observe the feasibility of the anode electrode. Experiment including Fourier transform infrared spectroscopy (FTIR) to analyze the distribution of functional groups after modification of the carbon cloth electrode, and through the comparison of the ultraviolet–visible spectrometer (UV–Vis), it can be known that the concentration ratio of [MR-CS] is 1:5, the glucose oxidase load can be maximized. Electrochemical analysis (Cyclic Voltammetry, CV) measures the activity of the maximum reaction of the anode material and the corresponding redox peak, and scanning electron microscope (SEM) observes the surface morphology of the modified electrode. Self-pumping glucose enzymatic biofuel cell module was assembled and examined, the results showed that the maximum output power density (MPD) was 2.64 mW/cm2.

Pemanfaatan Limbah Ikan Menjadi Pakan Bernutrisi Tinggi Solusi Inovatif Dalam Sektor Perikanan Di Kabupaten Kepulauan Selayar

Fishery waste consists of liquid and solid waste, accounting for 30-40% of the total weight of fish, mollusks, and crustaceans. Unfortunately, fish waste has not been well-managed due to its low economic value. However, with the advancement of technology, the opportunity to process fish waste into high-value products is increasingly open. Modern technology enables fish waste to be converted into high-quality fish feed, reducing the cost of commercial feed. The utilization of fish waste not only generates positive economic impacts but also significant environmental benefits. Processing fish waste into feed can reduce the risk of environmental pollution, decrease greenhouse gas emissions, and reduce pressure on wild fish resources. The use of feed from fish waste helps to promote more sustainable and environmentally friendly aquaculture practices. Research results show that using fish waste as an alternative feed in shrimp farming provides dual benefits for the environment and economy. Therefore, standard quality regulations are needed to ensure effective and safe management of fish waste for the environment and human health.

Fish Scales for Wearable Patches: Tailoring Films Assembled From Fish Waste Gelatin, Carbon Dots and Chitin Nanocrystals

AbstractThe properties of gelatin and collagen extracted from fishery wastes, specifically from mullet (Mugil Cephalus) scales, are modified with the aim to achieve biocompatible films with tunable characteristics to design sustainable UV protection solutions in the healthcare sector. Different additives are used to the scope including plasticizers, cross‐linkers, surfactants and optical modifiers. Carbon dots (CDs) obtained from seabass (Dicentrarchus labrax) scales combined with polysorbate‐20 (PS) as a dispersant agent, enable the fabrication of materials capable of blocking UV radiation almost completely, a crucial feature for dermal contact applications. The addition of hydrophobic surfactants and crosslinkers as citric acid, chitosan and surface deacetylated chitin nanocrystals (CsNCs) allowed to modulate the water adsorption of the films in the range 3–30% and to reach a twofold and 4.5‐fold increase of tensile strength and elastic modulus, respectively, as compared to the neat gelatin film. Moreover, film thickness is shown to be another adjustable parameter to enhance optic, mechanical, and permeation properties: the higher the thickness, the greater the UV‐blocking properties, elongation‐at‐break and water vapor permeability, leading to films with attractive characteristics as wearable patches.

Functional improvement and characterization of protein hydrolysates prepared by the fermentation of irradiated tilapia skin

Gamma irradiation (γ-irradiation) sterilizes and modifies proteins. This study aimed to enhance the performance of protein hydrolysates of fermented tilapia fish skin using irradiation. The results showed that γ-irradiation could effectively kill harmful microorganisms in fish skin at 6 kGy. Irradiation at 1–6 kGy dose disintegrated the internal structure of fish skin proteins, thus increasing the degree of hydrolysis after fermentation by Bacillus subtilis L4; however, irradiation at 9–12 kGy caused cross-linking and aggregation of fish skin proteins. The solubility, and water and oil holding capacity of the protein hydrolysates were significantly improved compared to those of the non-irradiated sample. The maximum emulsifying activity (164.61±16.28 m2/g), foaming activity (91.13±2.30 mL), and surface hydrophobicity index (5766.43±770.56) were observed in the protein hydrolysates fermented from fish skin irradiated at 12 kGy. The antioxidant activity of the protein hydrolysates increased with increasing irradiation doses. This suggested that the functional properties and antioxidant activity of protein hydrolysates from fish skin fermentation can be improved by appropriate irradiation pretreatment, providing a feasible method for improving the utilization and quality of proteins in fishery waste.

Production of chitosan from Aspergillus niger and quantitative evaluation of the process using adapted analytical tools

The chitosan production process from fishery waste is already established in industrial scale, whereby fungal chitosan is produced in lower amounts. Since fungal chitosan could be isolated from under-valorized vegan streams while exhibiting slightly different characteristics, it has also potential for other applications. Within this publication, we focus on the chitosan production from Aspergillus niger. This study provides a detailed determination of the biomass composition, adapting and comparing different analytical tools, with special focus on the chitin and chitosan content. The major content of the dried biomass is composed of glucans (48.6 ± 1.4%), followed by proteins with an amount of 22.2 ± 0.7%. Chitin and chitosan provide 16.0 ± 0.8% of the biomass. Within our chitosan production studies, we compared the effect of different process strategies including steps as deproteinization (DP), acid extraction (AE), deacetylation (DA), as well as purification. Initially, we obtained poor values (lower than 73.6%) for the chitosan purity. A direct DA step followed by purification resulted in a chitosan purity of up to 89.6%, a recovery of 30.5% and a yield with regard to the biomass of 5.5%. The DA degree of the resulting chitosan is similar to chitosan derived from fishery waste, whereas the molecular weight is lower. The results achieved so far are consistent with the literature, extending beyond, the data emphasized that a chitosan production from residual fungal biomass after fermentation is suitable by direct DA and purification. However, further adaption is necessary so that other matrix compounds could be also obtained.

Gradient heating activated ammonium persulfate oxidation for efficient preparation of high-quality chitin nanofibers

APS is a cheap and eco-friendly oxidant which enables one-step extraction of nanochitin (NCh) from fishery wastes. However, it is challenging to improve the preparation efficiency and NCh quality simultaneously, owing to the uneven or uncontrollable oxidation. Herein, we propose a simple and controllable way to isolate chitin nanofibers (ChNFs) from squid pen by gradient heating activated (GHA)- ammonium persulfate (APS) oxidation. Compared to the isothermal activated (ITA)-APS oxidation, our strategy reduced the mass ratio of squid pen to APS from 1:45 to 1:6 and reaction time from 15 h to 8 h. Meanwhile, the as-prepared ChNFs exhibited high yield (91.5 %), light transmittance (98 % at 500 nm), crystallinity index (96.9 %), and carboxyl content (1.53 mmol/g). GHA-APS oxidation involved multiple continuous heating and isothermal stages. The former stimulates a moderate activation of APS and enhances the oxidation rate, while the latter provides a duration for surface chemistry. This non-isothermal heating facilitates the continuous decomposition of APS at a relatively high and consistent rate, thereby enhances its oxidation efficiency. Furthermore, green assessments indicate this method is simple, time-saving, eco-friendly and cost-effective. Overall, this work introduces a novel perspective for the industrial extraction of high-efficiency and high-quality nanomaterials.

Adsorption properties of Pb(II) and Cd(II) in acid mine drainage by oyster shell loaded lignite composite in different morphologies

A new idea to alleviate environmental pollution is the development of low-cost adsorbents using natural minerals and fishery wastes to treat high concentrations of heavy metal pollutants in acid mine drainage (AMD). Adsorbent morphology, adsorptive and regenerative capacity, and application potential are limiting factors for their large-scale use. Oyster shells capable of releasing alkalinity were loaded on the surface of lignite to develop two composite adsorbents with different morphologies (powdery and globular) for the treatment of AMD containing Pb(II) and Cd(II). The results show that the ability of the adsorbent to treat AMD is closely related to its morphologies. The pseudo-second-order kinetic model and the Langmuir model are suitable to describe the adsorption process of OS-M(P), and the maximum adsorption saturation capacities of Pb(II) and Cd(II) are 332.6219 mg/g and 318.9854 mg/g, respectively. The pseudo-second-order kinetic model and the Freundlich model are suitable to describe the adsorption process of OS-M(G). A synergistic result of electrostatic adsorption, neutralization precipitation, ion exchange and complex reaction is achieved in the removal of Pb(II) and Cd(II) by two morphologies of adsorbents. The regeneration times (5 times) and recovery rate (75.75%) of OS-M(G) are higher than those of OS-M(P) (3 times) and recovery rate (20%). The ability of OS-M(G) to treat actual AMD wastewater is still better than that of OS-M(P). OS-M(G) can be used as a promising environmentally friendly adsorbent for the long-term remediation of AMD. This study provides a comprehensive picture of resource management and reuse opportunities for natural mineral and fishery wastes.

Turning fishery waste into aquafeed additives: Enhancing shrimp enzymes immobilization in alginate-based particles using electrohydrodynamic atomization

Alternative protein ingredients are gradually replacing fish meal in aquafeeds. However, farmed animals often struggle to digest the provided feed, which leads to inefficient nutrient assimilation. This results in a high feed conversion ratio and in water quality deterioration. Fish waste-derived proteases show great potential as bioactive ingredients to enhance protein digestibility. To achieve this, a suitable carrier is essential to include exogenous proteases in the fish feed formulation. Therefore, this research aimed to immobilize a shrimp enzyme extract into different alginate-based microcapsules through electrodynamic atomization and compare their structural and functional properties. Morphology, physicochemical and thermal properties, and in vitro protein release were evaluated for the following microcapsules made from various materials: alginate (A), alginate-chitosan (AC), alginate-bentonite (AB), alginate-bentonite previously subjected to a sonication treatment (ABs), and a combination of all materials (ABsC or ABC). The average sizes ranged from 360 to 790 μm (wet) and 260–516 μm (freeze-dried), confirming the expected differences, with larger sizes for those including chitosan and bentonite. ABsC proved to be the most suitable system for immobilizing exogenous proteases, as these particles exhibited high encapsulation efficiency in both wet and freeze-dried versions and displayed controlled protein release under typical conditions of the proximal intestine. This was further confirmed through an in vivo trial. Two bioassays were conducted with tilapia (Oreochromis niloticus) (n = 42, 2.6 ± 0.58 g) to evaluate the distribution of ABsC-rodamine labeled particles in digestive organs and digestive enzyme activity. ABsC remained in the intestine for over 7 h, and a significant increase in total proteolytic activity was observed 60 min after feeding. These findings suggest that ABsC microcapsules could serve as effective carriers for exogenous proteases in feeds for aquaculture.

Chitosan-Silica Composite Aerogel for the Adsorption of Cupric Ions.

A chitosan-silica hybrid aerogel was synthesized and presented as a potential adsorbent for the purification of cupric ion-contaminated media. The combination of the organic polymer (chitosan), which can be obtained from fishery wastes, with silica produced a mostly macroporous material with an average pore diameter of 33 µm. The obtained aerogel was extremely light (56 kg m-3), porous (96% porosity, 17 cm3 g-1 pore volume), and presented a Brunauer-Emmett-Teller surface area (SBET) of 2.05 m2 g-1. The effects of solution pH, aerogel and Cu(II) concentration, contact time, and counterion on cupric removal with the aerogel were studied. Results showed that the initial pH of the cation-containing aqueous solution had very little influence on the removal performance of this aerogel. According to Langmuir isotherm, this material can remove a maximum amount of ca. 40 mg of cupric ions per gram and the kinetic data showed that the surface reaction was the rate-limiting step and equilibrium was quickly reached (in less than one hour). Thus, the approach developed in this study enabled the recovery of waste for the preparation of a novel material, which can be efficiently reused in a new application, namely water remediation.

Exploring fishery waste potential as antifouling component.

Marine biofouling is a global issue with economic and ecological implications. Existing solutions, such as biocide-based antifouling paints, are toxic for the environment. The search for better antifouling agents remains crucial. Recent research focuses on eco-friendly antifouling paints containing natural compounds like enzymes. This study evaluates enzymatic extracts from fishery residues for antifouling potential. Extracts from Pleoticus muelleri shrimp, Illex argentinus squid, and Lithodes santolla king crab were analyzed. Proteolytic activity and thermal stability were assessed, followed by bioassays on mussel byssus thread formation and barnacle cypris adhesive footprints. All three extracts demonstrated proteolytic activity and 24-h stability at temperate oceanic temperatures, except I. argentinus. P. muelleri extracts hindered cyprid footprint formation and mussel byssus thread generation. Further purification is required for L. santolla extract to assess its antifouling potential activity. This study introduces the use of fishery waste-derived enzyme extracts as a novel antifouling agent, providing a sustainable tool to fight against biofouling formation.

Potential of Johnius belangerii and Thryssa hamiltonii as fishmeal in Deli Serdang, North Sumatera, Indonesia

An increasing problem in the Indonesian fishing industry is the scarcity of fish feedstuffs due to limited availability of raw materials, rising production costs, and climate change. Fishmeal are crucial in farms as they contain high-quality nutrients that are easily digestible by livestock. This study aims to observe the potential of the region as a producer for layer feedstuffs from fishmeal. This research used 10 kg of fresh Johnius belangerii’s fish and Thryssa hamiltonii’s fish with 5 kg each. The heads of both types of fish were collected to calculate their rendement. The results showed that the rendement percentage of Thryssa hamiltonii’s fish heads was 38.64%, while the yield percentage of Johnius belangerii fish heads was 36.83%. Based on the results it can be concluded that this region has significant potential in fishmeal from fisheries waste. 3 warehouses, Deli Serdang Regency, particularly Pantai Labu, can produce 19,108 kg of Thryssa hamiltonii fish in a year, utilizing 615.1 kg of head waste, 299 kg of offal waste, and 30.14 tons of protein. Johnius belangerii fish, weighing 20,299 kg with 623 kg of waste and 20.8 tons of protein content, are also produced in Deli Serdang Regency.

Characteristics of Compost from Straw and Fisheries Waste: A Comparative Analysis of Different Decomposers

The objective of this research was to assess the efficacy of three different decomposers in producing compost of high quality that meets the compost maturity standards (SNI 19-7030-2004). The experimental study was conducted with three treatments, each replicated five times, resulting in a total of fifteen composting holes. The research was conducted in South Batu Kuta Village, Narmada District, West Lombok Regency, from October to December 2019. The treatments included D1 (EM4 decomposer), D2 (Promi decomposer), and D3 (Phosphate Solubilizing Bacteria (PSB) decomposer). Various parameters, such as color, smell, temperature, pH, C-Organic, Total N, and C:N ratio, were assessed. The findings demonstrated that all three decomposers effectively decomposed the compost, yielding a high-quality product suitable for various plant applications. The compost produced by EM4, Promi, and PSB decomposers exhibited characteristics such as a blackish color, soil-like smell, a temperature ranging from 29.2°C to 29.8°C, a pH ranging from 6.83 to 6.87, organic carbon content ranging from 12.87% to 14.73%, total nitrogen content ranging from 0.83% to 0.87%, and a C/N ratio ranging from 14.35% to 14.60%. These results align with the compost maturity standards specified in SNI 19-7030-2004.

New discovery of extremely high adsorption of environmental DNA on cuttlefish bone pyrolysis derivative via large pore structure and carbon film

Environmental DNA (eDNA) carrying antibiotic resistance gene (ARG) has attracted a great deal of attention because of its threat to the ecology and human health. Traditional porous adsorbents, such as microporous biochar and natural mineral, are low-effective in removing eDNA from sewage. This study used cuttlefish-bone (CB), a fishery waste, as an anticipated material to adsorb a model compound of eDNA from herring sperm (hsDNA). An interesting result was firstly observed that extremely high DNA adsorption on cuttlefish-bone pyrolysis derivative (CCB) was up to 88.7 mg/g, 3–10 folds higher than that of most other adsorbents in the existing literatures, which was attributed to the carbon film and large pores. To achieve an adsorption rate of 75 %, hsDNA adsorption took 96 h on CB but only 24 h on CCB, which was attributed to the fluent channel of CCB. The ligand exchange, Ca2+ bridge and π-π interaction were identified as dominated adsorption mechanisms, based on FTIR and phosphate competition experiments. This study exploited a high-efficient, environmentally friendly, and low-cost adsorbent for treating ARG-contaminated soil and water.

Exploring the Transformation of Food Waste into Bioplastic Materials: A Review

Plastics, commonly encountered in forms such as packaging, household items, office supplies, electronics, and more, are typically conventional plastics derived from petroleum synthesis. The environmental issues posed by conventional plastics, which do not naturally degrade, necessitate a solution. An effective approach to address this challenge involves substituting conventional plastics, synthesized from petroleum, with biodegradable plastics made from natural materials. This article employs a literature review methodology, exploring research journals that cover a spectrum of bioplastics topics, including definitions, applications, advantages, natural ingredients, and final bioplastic products. Bioplastics, crafted from natural materials, possess the ability to decompose through microbial action, rendering them more environmentally friendly. Utilizing agricultural and fishery waste as sources of natural materials, such as cassava peels, fruit seeds, seaweed, fish scales, starch and cellulose content for bioplastic production. Bioplastic applications extend across various sectors, encompassing packaging, catering, electronics, automotive, agriculture, horticulture, toys, textiles, and more.

Efficacy of phosphorus loaded oyster shell in heavy metal removal: A combined study on adsorption behavior, structure characterization and comparative mechanism

This study presents a comprehensive evaluation of unmodified and modified oyster shells (OS, MP-OS, MC-OS) as adsorbents for removing heavy metals (Cd2+ and Pb2+) from water. The modifications involved phosphoric acid treatment (MP-OS) and dipotassium phosphate calcination (MC-OS), aiming to enhance the adsorption capabilities of the shells. Experimental results demonstrated that MC-OS exhibited the highest adsorption capacity due to its optimal pore structure and surface properties. The kinetic and isotherm studies revealed that the adsorption process is predominantly governed by chemical adsorption, with notable contributions from physical adsorption mechanisms. Morphological and surface analyses, including SEM, XRD, and FTIR, highlighted significant changes in the shells post-modification, contributing to their enhanced adsorption performance. The study also compared the environmental and economic aspects of these modified shells with other adsorbents like hydroxyapatite and reed biochar. The findings suggest that modified oyster shells, particularly MC-OS, are highly effective, eco-friendly, and economically viable for heavy metal remediation in water, with potential applications in soil decontamination. This research offers valuable insights into the utilization of fishery waste for environmental management, demonstrating the effectiveness of chemical modification in transforming natural biowastes into potent adsorbents.

Commercial Feed Substitution with Milkfish Waste Flour (Chanos chanos) Against Quail (Coturnix coturnix japonica) Egg White Index, Egg Yolk Index and Haugh Unit

Quail farming is quite developed, from year to year the population increases. The search for alternative feed as a substitute for feed sources is an effort by breeders to minimize feed costs. One alternative to replace feed ingredients can be fishery industry waste. Fishery waste that can be used as feed is milkfish waste (Chanos chanos). The purpose of this research was to determined the substitution of milkfish waste flour (Chanos chanos) at commercial feed to egg white index, egg yolk index and haugh unit. There are 20 quails (Coturnix coturnix japonica) at one day of age of four treatments and five replications. Each treatment consisted by five females. The treatment consisted of P0 with commercial feed 100%, P1 with commercial feed 98% + milkfish waste flour 2%, P2 with commercial feed 96% + milkfish waste flour 4% and P3 with commercial feed 94% + milkfish waste flour 6%. Based on results of the Analysis of Variance (ANOVA) there was not significantly different (p>0,05) on egg white index, egg yolk index and haugh unit. It can be concluded the substitution of commercial feed with milkfish waste flour (Chanos chanos) can maintain egg white index egg yolk index and haugh unit.

Pembelajaran Pembuatan Pakan Mandiri dan Penstimulan Moulting Udang bagi Pembudidaya Tradisional

Shrimp farming in Indonesia is dominated by traditional cultivation methods, therefore they do not have sufficient financial access to obtain expensive commercial feed. The Program for Application of Appropriate Technology (PTTG) aims to provide lessons on making independent feed and stimulating shrimp moulting for traditional farmers in Ma'rang District, Pangkajene and Islands Districts using local raw materials. The Participatory Rural Appraisal (PRA) approach model, which emphasizes the active involvement of the community in all activities, is used in this program. The knowledge and skills transferred from this study include the concept of artificial feed and moulting, preparation of raw materials, preparation of feed composition, mixing and weighing of raw materials, and pelleting and supplementation of feed. The results of the pre-test showed that the level of knowledge and skills regarding self-sufficient feed production and the manufacture of moulting-stimulating ingredients were generally in the poor category. However, after the learning activities, the post-test results showed that the level of knowledge and skills increased significantly and was generally in the good category. In general, partners believe that learning items are technically easy to apply, economically inexpensive, and ecologically can reduce agricultural and fishery waste. The lessons learned by traditional cultivators through this program can be utilized optimally in increasing production.

Boiling water resistant fully bio-based adhesive made from maleated chitosan and glucose with excellent performance

Biomass resources are widely considered potential alternatives to formaldehyde-based wood adhesives because of their abundance. In this study, an environmentally friendly biomass adhesive, carboxylated chitosan-glucose (CSC-G), was prepared using chitosan, maleic anhydride, and glucose. The structure and water resistance of the adhesive were analyzed in detail. Maleic anhydride act as a bridge connecting chitosan and glucose, giving the adhesive good water solubility and resistance. The improved water resistance of the CSC-G adhesive was attributed to the formation of covalent cross-linked structures and an increased degree of system cross-linking. Additionally, the curing temperature of the CSC-G adhesive was superior to those of previously reported polyester adhesives. This study not only expands the application scope of fishery waste, but also demonstrates its great potential for the preparation of high-performance plywood.