Fisheries Research Institute Research Articles

Fish community structure and its relationship with environmental factors in Leizhou Bay

PDF HTML阅读 XML下载 导出引用 引用提醒 雷州湾附近海域鱼类群落结构及其与环境因子的关系 DOI: 作者: 作者单位: 1. 广东海洋大学水产学院, 广东 湛江 524088;2. 中国水产科学研究院南海水产研究所, 农业农村部南海渔业资源开发利用重点实验室, 广东省渔业生态环境重点实验室, 广东 广州 510300 作者简介: 曾嘉维(1992-),男,硕士研究生,从事渔业生态学研究.E-mail:zengjw1992@163.com 通讯作者: 中图分类号: S931 基金项目: 公益性行业（农业）科研专项经费项目（201403008）. Fish community structure and its relationship with environmental factors in Leizhou Bay Author: Affiliation: 1. Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China;2. Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs;Guangdong Provincial Key Laboratory of Fishery Ecology and Environment;South China Sea Fisheries Re-search Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:为了解雷州湾及其附近海域渔业生物的群落结构及其物理影响因素，于2016—2017年开展了4个季度的底拖网渔业资源与生态环境调查。运用聚类分析、相似性百分比分析以及冗余分析研究了该海域鱼类群落结构及其与环境因子之间的关系。结果表明，雷州湾附近海域共捕获鱼类256种，以底层鱼类和暖水性鱼类为主；各季节均可划分为两个亚群落（Anosim test：Cynoglossus puncticeps）、中华海鲇（）等；群落Ⅱ的平均相似性为41.38%~52.59%，主要贡献种为多齿蛇鲻（）等。两个亚群落的平均相异性为57.74%~76.25%；水深是4个季度显著影响鱼类分布的重要生态因子，溶解氧是3个季度显著影响鱼类分布的重要环境因子。综上所述，雷州湾及其附近海域鱼类种类数丰富，高于海州湾、大亚湾等海域；本研究揭示了该海域鱼类群落结构特征及其与环境因子的关系，为该海域鱼类资源的养护及利用提供参考依据。 Abstract:Leizhou Bay, located on the eastern side of Leizhou Peninsula in Guangdong province, is the primary habitat of Chinese white dolphin (), besides being a reserve for juvenile fish and shrimp. However, the ecological state of this coastal ecosystem remains largely unknown. To understand the fish community structure and its physical drivers in Leizhou Bay, seasonal bottom trawl along with environment monitoring surveys were conducted from 2016 to 2017. The relationships of fish community structure with environmental factors were analyzed using K-means cluster analysis, similarity percentage analysis, and redundancy analysis. These analyses led to the identification of 256 fish species, comprising mainly demersal fish and warm-water fish. The study sites for each season could be divided into two groups:group I, with water depth of less than 10 m, and group Ⅱ, with water depth between 10 m and 20 m. Group I showed an average similarity of 38.48%-47.44% across seasons in terms of fish community structure (which was shown as matrixes of sites by species biomass). Three species, Plotosus lineatus contributed greatly to the average similarity within Group I in summer, fall and winter. Furthermore, Group Ⅱ showed an average similarity of 41.38%-52.59% across seasons. The contributed mostly to the average similarity within Group Ⅱ in spring, summer and winter. The average dissimilarity between group I and group Ⅱ in seasons was 57.74%-76.25%. Six ecological factors, i.e. depth, dissolved oxygen, water temperature, salinity, pH, and chlorophyll a, explained 41.76%-47.11% of variation in fish biomass, with depth being the dominant environmental factor affecting fish community structure in each season. Moreover, the fish species richness in Leizhou Bay was obviously higher than in other bays of China. This study provides preliminary data on fish community structure and its relationship with environmental factors in Leizhou Bay, and provides clues for stocking enhancement in the waters. 参考文献 相似文献 引证文献

Comparative analysis of embryonic muscle development in wildtype zebrafish and its intermuscular bone deficiency mutant

PDF HTML阅读 XML下载 导出引用 引用提醒 肌间刺缺失突变对斑马鱼胚胎发育过程中肌肉发育的影响 DOI: 作者: 作者单位: 1. 上海海洋大学, 水产科学国家级实验教学示范中心, 上海 201306;2. 中国水产科学研究院黑龙江水产研究所, 黑龙江 哈尔滨 150070 作者简介: 杨建(1987-),男,博士研究生,主要从事鱼类遗传育种的研究.E-mail:last0templar@126.com 通讯作者: 中图分类号: S917 基金项目: 中国水产科学研究院基本科研业务费项目（2016HY-ZD0301）. Comparative analysis of embryonic muscle development in wildtype zebrafish and its intermuscular bone deficiency mutant Author: Affiliation: 1. National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China;2. Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:为阐明肌间刺缺失对斑马鱼（、）在囊胚期（3 hpf，hours post fertilization）、原肠胚期（6 hpf）、体节期（12 hpf）、咽囊期（24 hpf）和孵化期（72 hpf）5个发育时期的表达情况，对比分析了肌间刺缺失对斑马鱼胚胎发育过程中肌肉发育特异性基因的时序表达差异和肌肉发育的影响，以及对受精率、孵化率和畸形率的影响。结果显示，5个特异性基因在肌间刺缺失突变型与野生型斑马鱼各发育时期的表达量无显著性差异（、基因在囊胚期和原肠胚期表达量很低，在体节期和咽囊期表达量迅速升高，并在孵化期开始下降。此外，受精率、孵化率和畸形率的统计结果显示，肌间刺突变型与野生型间亦无显著性差异（>0.05）。综上，推测肌间刺的缺失对斑马鱼胚胎发育及胚胎发育过程中肌肉的发育没有影响。 Abstract:In order to explore the difference in embryonic muscle development between an intermuscular bone (Imb) deficiency mutant and wildtype zebrafish, we used a qRT-PCR method to analyze the expression of 5 muscle-specific genes, including , in the development stages blastula (3 hpf, hours post fertilization), gastrula (6 hpf), segmentation (12 hpf), pharyngula (24 hpf), and hatching period (72 hpf), and discussed the impact of Imb's deficiency on muscle development during embryonic development. Furthermore, the differences in fertilization rate, hatching, and abnormal rate of larvae were assessed. The results showed that the expression of genes were elevated in the gastrula stage, and downregulated in the segmentation and pharyngula stages; the expression levels of in the blastula and gastrula stages were extremely low, and were upregulated significantly in the segmentation and pharyngula stages before being downregulated in the hatching period. The results also showed that there were no significant differences in 5 muscle-specific genes' expression between the wildtype zebrafish and its Imb deficiency mutant. Additionally, no significant difference in fertilization rate, hatching, and abnormal rate occurred between the two Imb phenotypes. In summary, the deletion of Imb do not impact muscle development or the survival of embryos during embryonic development of zebrafish. 参考文献 相似文献 引证文献

Temporal-spatial characteristics of lipea gene expression in Cyprinus carpio and its correlation with fat deposition

PDF HTML阅读 XML下载 导出引用 引用提醒 鲤lipea基因表达的时空特征及与脂肪沉积相关性分析 DOI: 作者: 作者单位: 1. 上海海洋大学, 农业农村部淡水水产种质资源重点实验室, 上海 201306;2. 中国水产科学研究院黑龙江水产研究所, 淡水鱼类育种国家地方联合工程实验室, 黑龙江 哈尔滨 150070 作者简介: 党红阳(1995-),女,硕士研究生,研究方向为水产生物技术.E-mail:danghongyang95@163.com 通讯作者: 中图分类号: S965 基金项目: 国家自然科学基金项目（31302174）；中国水产科学研究院基本科研业务费（2015C04XK01）；国家水产种质资源共享服务平台项目（2018DKA30470）. Temporal-spatial characteristics of lipea gene expression in Cyprinus carpio and its correlation with fat deposition Author: Affiliation: 1. Key Laboratory of Freshwater Fishery Germplasm Resource, Ministry of Agriculture and Rural Affairs;Shanghai Ocean University, Shanghai 201306, China;2. National Local Joint Engineering Laboratory for Freshwater Fish Breeding, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:本文旨在克隆鲤（）基因，探究其时空表达特征及其与脂肪沉积的关系，以期为鲤脂肪沉积分子调控机制研究奠定理论基础。本实验采用RACE技术克隆获得鲤基因，其cDNA全长为3379 bp，包括230 bp的5'非翻译区（5'-UTR）、1067 bp的3'非翻译区（3'-UTR）和编码693个氨基酸的开放阅读框。多序列比对显示，鲤Oncorhynchus mykiss）的氨基酸序列相似性分别为88.17%、64.94%和63.35%，不同物种间序列差异主要集中在C-端的多肽序列上。蛋白质结构分析显示该蛋白含有3个功能域分别具有脂肪酶活性、乙酰基水解酶活性和水解酶活性。应用实时荧光定量PCR技术分析了在各组织中均有表达，腹腔脂肪中相对表达量最高，其次是腹部肌肉，血液中最少。随着胚胎的发育，表达量与背部肌肉脂肪含量呈正相关，但差异不显著（基因对鲤腹腔脂肪沉积可能具有抑制作用。本实验结果可为进一步解析鲤基因的功能和基因调控脂肪沉积分子机制研究提供参考。 Abstract:The objectives of this study were to investigate the expression pattern of Cyprinus carpio), to lay the foundation for studying and understanding the molecular regulation mechanism of fat deposition in common carp. In this study, a full-length cDNA sequence of gene in common carp was cloned by reverse transcription PCR and rapid-amplification of cDNA ends (RACE) technology, and the expression pattern of gene in different tissues of common carp and during its different developmental stages. cDNA of the gene of common carp was 3379 bp, with an open reading frame of 2079 bp, 5' untranslated region was 230 bp, 3' untranslated region was 1067 bp, encoding 693 amino acids. Multiple sequences alignment revealed that the amino acid sequences of Paralichthys olivaceus, and , respectively. It was determined that the sequence differences were mainly concentrated on the C-terminal polypeptide sequence between different species, and the structural analysis of proteins showed that the protein contains three functional domains with lipase activity, acetyl hydrolase activity, and hydrolase activity, respectively. Real-time quantitative PCR showed that the gene was expressed in all tested tissues, and the relative expression level was the highest in visceral fat, followed by abdominal muscles, and lowest in the blood. Its expression declined during the development of embryo; it was the highest in the 8-cell stage, followed by the late blastula stage. It was the lowest in the feeding period. The mRNA expression in different fat tissues negatively correlated with adipose tissue in the abdominal cavity; the highest and the lowest expression levels differed significantly (<0.01). The expression also positively correlated with dorsal muscle but it was not significant ( gene highlights its function and molecular regulation mechanism of fat development in common carp. 参考文献 相似文献 引证文献

Genetic differentiation of the purpleback flying squid, Sthenoteuthis oualaniensis , in the South China Sea:population or species divergence

PDF HTML阅读 XML下载 导出引用 引用提醒 南海鸢乌贼的遗传差异: 种群分化还是种间分化 DOI: 作者: 作者单位: 中国水产科学研究院南海水产研究所, 农业农村部外海渔业开发重点实验室, 广东 广州 510300 作者简介: 李敏(1984-),男,博士,副研究员,从事海洋生物多样性和保护研究.E-mail:limin@scsfri.ac.cn 通讯作者: 中图分类号: S931 基金项目: 国家重点研发计划项目（2018YFC1406500）；农业农村部财政专项（NFZX2018）；广东省自然科学基金项目（2014A030310177）；国家重点基础研究发展计划项目（2014CB441500）. Genetic differentiation of the purpleback flying squid, Sthenoteuthis oualaniensis, in the South China Sea:population or species divergence Author: Affiliation: Key Laboratory of Open-Sea Fishery Development, Ministry of Agriculture and Rural Affairs;South China Sea Fisher-ies Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:利用线粒体ND2、COI和16S rDNA序列，对2012年9月至2015年9月采自南海的鸢乌贼（）样本进行了种群遗传结构的分析。系统发育关系显示鸢乌贼中型群和微型群各自形成了单系群分支，而分支内没有形成与地理群体或季节群体对应的支系。分子方差分析同样表明，地理群体或季节群体之间不存在明显的遗传分化，而中型群和微型群之间具有极显著的遗传分化（<0.0001）。遗传多样性分析显示，鸢乌贼中型群和微型群均呈现出较高的单倍型多样性（0.9465和0.8545）和较低的核苷酸多样性（0.0051和0.0021），中型群的遗传多样性指数要高于微型群。基于ND2、COI和16S rDNA序列计算的中型群和微型群之间的遗传距离分别达到了14.0%、9.6%和8.8%，远大于类群内的遗传分化（平均<1%）。研究结果表明，南海鸢乌贼存在分布高度重合且有显著遗传分化的两个种群，即中型群和微型群。中型群和微型群之间的遗传差异达到了种间分化的水平，提示体型较大、具有背部发光器的中型群鸢乌贼（典型鸢乌贼）与体型较小、背部发光器缺失的微型群鸢乌贼可能是两个相互独立的种类。 Abstract:In the present study, the population differentiation of the purpleback flying squid () in the South China Sea was examined based on mitochondrial ND2, COI, and 16S rDNA sequences, using samples collected between September 2012 and September 2015. Phylogenetic analysis revealed two significantly divergent monophyletic groups corresponding to the "medium form" and the "dwarf form, " but no geographical lineages corresponding to the sampling localities or seasonal groups were found. The Analysis of Molecular Variance also revealed significant genetic differentiation between the "medium form" and the "dwarf form" (<0.0001), while no genetic variance between either geographic populations or seasonal groups was found. The genetic diversity analysis showed that both the "medium form" and the "dwarf form" of in the South China Sea were characterized by high haplotype diversity (0.9465 and 0.8545) and low nucleotide diversity (0.0051 and 0.0021). The genetic distance between the ‘medium form’ and the "dwarf form" based on ND2, COI, and 16S sequences were 14.0%, 9.6%, and 8.8%, respectively. The genetic distances between the two forms were considerably larger than the genetic distances within each of the two forms (<1%). It was demonstrated that two divergent populations of were sympatrically distributed in the South China Sea, i.e. the "medium form" and the "dwarf form." The genetic differentiation between these two populations was similar to that among separate species within Ommastrephidae, which indicated that the two morphologically distinct forms of the purpleback flying squid, namely the "medium form" (middle sized, with a photophore) and the "dwarf form" (small sized, without a photophore), are two distinct species. 参考文献 相似文献 引证文献

Codend selectivity in the East China Sea of a trawl net with the legal minimum mesh size

Selectivity curves were obtained for 22 species of fish and squid from stock assessment research data for the East China Sea between 2001 and 2011, conducted using a cover net attached to the codend of a trawl net (Seikai National Fisheries Research Institute SS-RI-type trawl net). The trawl net codend was made of diamond mesh net with a legal minimum mesh opening size of 54 mm (mesh length of 66 mm). A cover net with a mesh opening of 18 mm (or 10.3 mm depending on the research year) was attached to the codend. For each of the 20 fish species and two squid species, we pooled data of hauls where body size for the whole catch was measured without subsampling to obtain the body size compositions of fish caught in both the codend and the cover net. The maximum likelihood method was performed for estimation of parameters in the logistic curve equation representing the codend selection curve. For 18 fish species (excluding Trichiurus japonicus and Muraenesox cinereus), we examined the relationship of the obtained selection parameters [length at 50% retention (l50) and selection range (SR) (= l75–l25)] to fish body shape. We demonstrated that, in fish species with a smaller ratio of body height/width to body size (i.e., more slender body type), there was a tendency for larger l50 and SR. Furthermore, by comparing the l50 of each fish species with reproductive parameters such as minimum length at maturity, we examined the sustainability of the resources based on the minimum mesh size regulation.

Carbon budgets from mariculture ponds without a food supply

AEI Aquaculture Environment Interactions Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsTheme Sections AEI 10:465-472 (2018) - DOI: https://doi.org/10.3354/aei00279 Carbon budgets from mariculture ponds without a food supply Yan Chen1,2, Shuanglin Dong2,*, Yucen Bai3, Shaogang Xu1, Xiaofei Yang1, Zhe Pan2 1Beijing Fisheries Research Institute, Beijing 100068, China 2Key Laboratory of Mariculture, Ministry of Education of China. Ocean University of China, Qingdao 266003, PR China 3China Rural Technology Development Center, 54 Sanlihe Road, Beijing 100045, PR China *Corresponding author: dongsl@ouc.edu.cn ABSTRACT: To estimate the carbon source-sink function of mariculture ponds without a food supply, we analyzed carbon budgets and gaseous carbon fluxes in shrimp Marsupenaeus japonicus-sea cucumber Apostichopus japonicus polyculture seawater ponds (PSPs) without feeding. Over a 1 yr cycle, the total organic carbon outflow accounted for 78.6% of the inflow organic carbon in PSPs. Approximately 1558.1 kg ha-1 of organic carbon accumulated in PSPs, and the ratio of gaseous carbon exchange to carbon burial ranged from 0.73 to 1.45. The PSPs acted as a purification system where organic carbon is eliminated from the inflow water, and carbon retention in the sediment of such systems could offset their carbon emissions into the atmosphere. KEY WORDS: Mariculture ponds · No-feeding · Methane · Carbon dioxide · Carbon budgets Full text in pdf format PreviousCite this article as: Chen Y, Dong S, Bai Y, Xu S, Yang X, Pan Z (2018) Carbon budgets from mariculture ponds without a food supply. Aquacult Environ Interact 10:465-472. https://doi.org/10.3354/aei00279 Export citation RSS - Facebook - Tweet - linkedIn Cited by Published in AEI Vol. 10. Online publication date: November 01, 2018 Print ISSN: 1869-215X; Online ISSN: 1869-7534 Copyright © 2018 Inter-Research.

Low genetic diversity in broodstocks of endangered Chinese sucker, Myxocyprinusasiaticus: implications for artificial propagation and conservation.

The releasing program of Chinese sucker (Myxocyprinusasiaticus) has been conducted for years in China. To prevent loss of genetic variation in wild populations, it is important to assess and monitor genetic diversity of broodstocks before release of offspring. Three broodstocks (Pixian Base of Sichuan Fisheries Research Institute, China (PBS), Yibin Base of Sichuan Fisheries Research Institute, China (YBS) and Yibin Rare Aquatic Animal Research Institute, China (YRA)) were investigated using mitochondrial control region and 12 microsatellites. The relatively low genetic diversities of these broodstocks were detected (PBS, haplotype diversity (h) = 0.877, observed heterozygosity (Ho) = 0.416; YBS, h = 0.812, Ho = 0.392; YRA, h = 0.818, Ho = 0.365). PBS showed higher Ho than YBS and YRA (P < 0.05). Genetic divergence (FST) based on microsatellites between PBS and YRA was significant (FST = 0.1270, P < 0.05), the same situation happened between YBS and YRA (FST = 0.1319, P < 0.05). However, divergence between PBS and YBS was not significant (FST = 0.0029, P > 0.05). Structure analysis revealed that YRA were distinct from PBS and YBS. Based on these results, it is important to propose some suggestions of genetic management for artificial propagation of Chinese sucker, such as broodstock exchange among hatcheries and broodstock supplement from wild.

Spatial-temporal dynamics of water hyacinth, Eichhornia crassipes (Mart.) and other macrophytes and their impact on fisheries in Lake Victoria, Kenya

Abstract Invasive aquatic macrophytes in Lake Victoria including water hyacinth (Eichhornia crassipes) exhibit periodic cyclical patterns of decline and proliferation with attendant ecological and economic impacts. This study aimed to monitor the extent of macrophytes and other invasive weeds on Lake Victoria to establish their impact on fisheries. The study employed a combination of remote sensing and geographical information system (GIS) techniques to estimate the coverage of water hyacinth and other invasive macrophytes. Data on fish landings and their respective market values were acquired from the Electronic Fish Market Information Service (EFMIS) database, which is hosted by the Kenya Marine and Fisheries Research Institute (KMFRI). Analysis of consistent temporal satellite data showed that the weeds frequently cover sheltered bays and river mouths. These areas of Winam Gulf had higher coverage (average 5000 ha) of macrophytes than the open waters (

Aberration of Metals Competing for Iron on Exposure to Lambda Cyhalothrin and Aflatoxins in Dietary Fish from Selected Aquatic Sources in Kenya

&lt;p&gt;&lt;em&gt;Excess or deficiency of minerals may seriously disturb biochemical processes and upset internal homeostasis, leading to various diseases and disorders in fish species due to deficiency or excess of micro and macro elements caused by improper nutrition, avitaminosis or poisoning. The specific objectives of the study were to determine the iron levels and aberration of metals competing on exposure to lambda-cyhalothrin and aflatoxins in dietary fish from selected aquatic sources in Kenya. The concentration of elements cadmium, zinc, and iron in Oreochromis niloticus and Clarias gariepinus bred in Kenya Marine and Fisheries Research Institute at Sagana and obtained from River Nyando was measured using atomic absorption spectrophotometer. Iron availability was lower on treatment with Aflatoxin compared to Lambda-Cyhalothrin with a mean of 3.66 ± 0.84 mg/kg, but on subjection to zinc, competition was 3.82 mg/kg on consideration of zinc competition. The naturally occurring toxins cause micronutrient deprival and therefore relevant stakeholders be keen to prevent contamination from farm to fork.&lt;/em&gt;&lt;/p&gt;

Taxonomic notes on Anthomedusae (Cnidaria: Hydrozoa: Hydroidomedusa) from the south-central South China Sea, with a new genus and four new species

This study describes and illustrates four new species Laticanna nanhaiensis Xu, Huang and Wang, gen. nov. and sp. nov., Protiaropsis gemmifera Xu, Huang and Du, sp. nov., Merga nanhaiensis Xu, Huang and Guo, sp. nov. and Rhabdoon apiciloculus Xu, Huang and Du, sp. nov. based on specimens from the south-central South China Sea. Additionally, keys to known genera of family Bythotiaridae and species of genera Protiaropsis, Merga, and Rhabdoon are described. All type specimens are deposited in the South China Sea Fisheries Research Institute, Chinese Academy of Fishery Science.

Spatio-temporal dynamics in the dissolved nutrient waste plume from Norwegian salmon cage aquaculture

AEI Aquaculture Environment Interactions Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsTheme Sections AEI 10:385-399 (2018) - DOI: https://doi.org/10.3354/aei00276 Spatio-temporal dynamics in the dissolved nutrient waste plume from Norwegian salmon cage aquaculture H. M. Jansen1,2,*, O. J. Broch3, R. Bannister1, P. Cranford4, A. Handå3, V. Husa1, Z. Jiang5, T. Strohmeier1, Ø. Strand1 1Institute of Marine Research, Nordnesgaten 50, 5005 Bergen, Norway 2Wageningen University and Research, Wageningen Marine Research (WMR), PO Box 77, 4400 AB Yerseke, The Netherlands 3SINTEF Ocean, Postboks 4762 Torgarden, 7465 Trondheim, Norway 4Fisheries and Oceans Canada, St. Andrews Biological Station, 125 Marine Science Dr., Saint Andrews, New Brunswick E5B 0E4, Canada 5Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao, Shandong Province, PR China *Corresponding author: henrice.jansen@wur.nl ABSTRACT: The aim of this study was to define the waste plume dynamics around a salmon farm in Norway. Systematic water sampling and numerical modeling were implemented to define nutrient concentrations in the upper water column at long-term (seasonal) and short-term (between and within days) time scales. Nutrient enhancement was observed for ammonium only, while the concentrations of orthophosphate and organic wastes were never higher than the background values. The spatial magnitude of cage effluent dispersion was limited. Empirical results detected enhanced concentrations up to 100 m down-current of the farm when fish biomass was high. Model results showed that the zone of influence could occasionally reach to >1000 m. In the first year of production, when fish biomass was low, no enhancement was detected, and in April and September of the following year, average ammonium concentrations were respectively 0.2 and 0.8 µM above the background concentrations. Taking the ambient seasonal variability into account, this resulted in 1.6 times higher concentrations for both sampling months. The measured short-term temporal variability in nutrient concentrations near the cages varied up to 2 times from day to day and were 3.5 times higher in the evening compared to the morning. As seasonal investigations were performed in the morning, maximum enhancement was likely underestimated. The rapid decrease in nutrient concentrations with increasing distance from the cages suggests that the farm studied here is currently not causing significant degradation of surface water quality. Results of this study contribute to evaluating the potential for ecological mitigation of waste nutrients and provide directions for design of optimized integrated multi-trophic aquaculture facilities. KEY WORDS: Atlantic salmon · Farm scale · Nutrients · Nitrogen · Integrated multi-trophic aquaculture · IMTA · Pelagic · Environmental impact Full text in pdf format Supplementary material PreviousNextCite this article as: Jansen HM, Broch OJ, Bannister R, Cranford P and others (2018) Spatio-temporal dynamics in the dissolved nutrient waste plume from Norwegian salmon cage aquaculture. Aquacult Environ Interact 10:385-399. https://doi.org/10.3354/aei00276 Export citation RSS - Facebook - Tweet - linkedIn Cited by Published in AEI Vol. 10. Online publication date: September 27, 2018 Print ISSN: 1869-215X; Online ISSN: 1869-7534 Copyright © 2018 Inter-Research.

Improved MODIS-Aqua Chlorophyll-a Retrievals in the Turbid Semi-Enclosed Ariake Bay, Japan

The accurate retrieval of chlorophyll-a concentration (Chl-a) from ocean color satellite data is extremely challenging in turbid, optically complex coastal waters. Ariake Bay in Japan is a turbid semi-enclosed bay of great socio-economic significance, but it suffers from serious water quality problems, particularly due to red tide events. Chl-a derived from the MODerate resolution Imaging Spectroradiometer (MODIS) sensor on satellite Aqua in Ariake Bay was investigated, and it was determined that the causes of the errors were from inaccurate atmospheric correction and inappropriate in-water algorithms. To improve the accuracy of MODIS remote sensing reflectance (Rrs) in the blue and green bands, a simple method was adopted using in situ Rrs data. This method assumes that the error in MODIS Rrs(547) is small, and MODIS Rrs(412) can be estimated from MODIS Rrs(547) using a linear relation between in situ Rrs(412) and Rrs(547). We also showed that the standard MODIS Chl-a algorithm, OC3M, underestimated Chl-a, which was mostly due to water column turbidity. A new empirical switching algorithm was generated based on the relationship between in situ Chl-a and the blue-to-green band ratio, max(Rrs(443), Rrs(448)/Rrs(547), which was the same as the OC3M algorithm. The criterion of Rrs(667) of 0.005 sr−1 was used to evaluate the extent of turbidity for the switching algorithm. The results showed that the switching algorithm performed better than OC3M, and the root mean square error (RMSE) of estimated Chl-a decreased from 0.414 to 0.326. The RMSE for MODIS Chl-a using the recalculated Rrs and the switching algorithm was 0.287, which was a significant improvement from the RMSE of 0.610, which was obtained using standard MODIS Chl-a. Finally, the accuracy of our method was tested with an independent dataset collected by the local Fisheries Research Institute, and the results revealed that the switching algorithm with the recalculated Rrs reduced the RMSE of MODIS Chl-a from 0.412 of the standard to 0.335.

Microsatellite Analysis of Genetic Variation in Intraspecific Hybrids and Self-Breds of Steelhead Trout and Golden Rainbow Trout

Genetic diversity of the hybrids and inbreds of Steelhead trout and golden rainbow trout, altogether four populations, was studied based on 15 polymorphic microsatellite locus. Two hundred and forty individuals (sixty individuals per one population) were collected from the Yudu hill aquaculture experimental station of Beijing Fisheries Research Institute, Beijing, China. The genetic variation of the hybrids and inbreds of Steelhead trout and golden rainbow trout were all relatively high. The mean observed heterozygosity value of YJ, YY, JJ and JY were respectively 0.6600, 0.7733, 0.7822, and 0.6533. The mean Fis and Fit value were respectively (-0.5065, -0.6037, -0.5728, -0.4620) and (-0.3857, -0.5272, -0.4987, -0.4069), showing an average excess of heterozygote proportion. The mean Fst value was respectively 0.0802, 0.0477, 0.0471, and 0.0376. The mean genetic distance between JJ and YY was the closest (0.0097), next to YJ and YY (0.0655), and that between JY and YY was the most distant (0.1096). The dendrogram showed JY and YJ firstly clustered together, and then they clustered together with the inbred offspring (JJ and YY). Duo to Fst (little genetic differentiation) and Nm (the Nm values of 15 locus > 1), JY was a finer variety the two hybrids of Steelhead trout and golden rainbow trout. Our data offer supporting evidence that inbreeding depression of Steelhead trout and golden rainbow trout involves multiple related genes, favoring partial dominance over dominance.

Knowledge and skill development of Bihar farmers on inland fisheries management: A terminal evaluation

The state of Bihar in India has a huge potential in fisheries and the research institutes of Indian Council for Agricultural Research (ICAR) are involved in building capacity of the fish farmers of the state. Since 2012-13 to 2014-15 about 1279 fish farmers from 21 districts of Bihar State were trained under the Department of Fisheries/Agricultural Technology Management Agency schemes by ICAR-Central Inland Fisheries Research Institute on inland fisheries management. An ex-post facto study was undertaken to measure the effectiveness of these training programmes. Data were collected from 400 trainees from six different districts of Bihar using a semi-structured interview schedule. The Training Effectiveness Index (TEI) was developed and the results of the study indicated that the logistic regression analysis with socio-economic variables like main occupation, land holding, membership in organisations had positive and significant effect on training effectiveness. The overall effectiveness of training was found to be 87.86% which was recorded under the high effectiveness category. The results also revealed that 44.8% of the trainees were young and they also had positive effect on training effectiveness. About 53% of the farmers perceived that the training situation provided to them was highly satisfactory. The farmers also perceived that these trainings helped them to increase their knowledge and skill, enhance entrepreneurial ability and also motivated them to initiate new enterprises.

Cage fish culture in Lake Victoria: A boon or a disaster in waiting?

AbstractFish catches in Lake Victoria have been on the decline while demand for fish has been increasing. Cage culture with &gt;3,000 cages, over 3 million tilapias and valued at US$ 12 million is trying to fill the gap. This study reviews the development of cages and the need to develop a decision support tool for effective management. Existing literature and data on fisheries and cage culture held by the Kenya Marine and Fisheries Research Institute (KMFRI) were used in the analysis. Cage culture is a promising venture that may increase productivity, offer employment and enhance economic well‐being. However, site suitability for the installation of cages is poorly regulated with most developments (&gt;45%) located within 200 m of the shoreline that are breeding grounds of fish and conflict with other lake users. Waste feed increases eutrophication, and enhances growth of algae and water hyacinth in the lake. Recent isolated fish kills were attributed to low dissolved oxygen concentrations (&lt;0.64 mg/L), and an increasing occurrence of fish disease are signs of poor management practices among cage farmers. Cage culture may turn into an environmental disaster if not managed well, and there is need for robust policies and increased awareness to reduce environmental impacts.

Service Quality of Libraries of Indian Council of Agricultural Research Institutes of Kerala, India

&lt;p&gt;The purpose of this study is to measure the service quality of the libraries of Indian Council of Agricultural Research institutes in Kerala. Survey method with modified SERVQUAL questionnaire was employed to elicit responses from a representative sample of 180 users of the libraries. It is revealed that the ICAR institute libraries lack service quality. While the libraries compared, Central Marine Fisheries Research Institute Library has shown quality to an extent and library of Central Tuber Crops Research Institute has shown least service quality. Library collection dimension was found as the most expected dimension whereas library staff dimension was the most perceived dimension. The highest service quality gap was identified with library service dimension and the lowest gap was identified with library staff dimension. Regular user need assessment and feedback mechanism should be done to improve the service quality of the libraries. The study can be utilised for developing quality enhancement practices in special libraries in India. &lt;/p&gt;

Association of Pseudomonas anguilliseptica with mortalities in cultured spotted halibut Verasper variegatus (Temminck &amp; Schlegel, 1846) in China

Association of <i>Pseudomonas anguilliseptica</i> with mortalities in cultured spotted halibut <i>Verasper variegatus</i> (Temminck &amp; Schlegel, 1846) in China

Comparative analysis of different DNA barcoding methods for Veneroida classification and identification

PDF HTML阅读 XML下载 导出引用 引用提醒 不同DNA条形码基因在帘蛤目贝类分类鉴定中的比较分析 DOI: 作者: 作者单位: 1. 中国水产科学研究院黄海水产研究所, 农业农村部海洋渔业可持续发展重点实验室, 山东 青岛 266071;2. 海洋渔业科学与食物产出过程功能实验室, 青岛海洋科学与技术国家实验室, 山东 青岛 266273;3. 水产科学国家级实验教学示范中心, 上海海洋大学, 上海 201306;4. 烟台市水产研究所, 山东 烟台 264000 作者简介: 吴彪(1982-),男,副研究员,从事贝类遗传育种与繁育研究.E-mail:wubiao@ysfri.ac.cn 通讯作者: 中图分类号: S917 基金项目: 科技部科技基础性工作专项（2013FY110700）；国家水产种质资源共享服务平台（2017DKA30470）. Comparative analysis of different DNA barcoding methods for Veneroida classification and identification Author: Affiliation: 1. Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs;Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;2. Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266273, China;3. National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China;4. Yantai Fisheries Research Institute, Yantai 264000, China Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:DNA条形码基因已经广泛应用在海洋贝类的分类鉴定、系统发育进化、种群遗传分析等领域的研究。为进一步研究评估不同DNA条形码基因在海洋贝类鉴定中的作用，本研究利用从GenBank数据库随机下载的帘蛤目COI、16S rRNA、18S rRNA和28S rRNA基因序列，通过传统距离法和单系聚类法结合分析，比较了上述DNA条形码基因在鉴定物种及系统发育进化中的鉴定效率，并以本实验室已获得的部分贝类DNA序列进行了验证。结果表明，根据“10倍法则”和“2%”阈值标准，本研究中COI能够鉴定57.1%物种，16S rRNA能够鉴定60.9%，18S rRNA鉴定16.7%，而28S rRNA无法有效鉴定；多数种COI和16S rRNA基因序列的种间遗传距离和种内遗传距离存在“条形码间隙”，而18S rRNA和28S rRNA序列的种间和种内的遗传距离存在显著重叠，没有明显“条形码间隙”；聚类分析结果表明，基于COI基因序列，87.9%的个体与同种聚为单系，以16S rRNA序列，65.6%的个体与同种聚为单系，未聚成单系的个体则形成姐妹系，未出现不同种聚为单系现象，能够呈现与形态分类基本一致的系统发生关系；但18S rRNA和28S rRNA呈现的聚类关系相对混乱。相对而言，在鉴定帘蛤目物种时，COI和16S rRNA都能够作为条形码基因，且COI有效性更高，18S rRNA和28S rRNA基因由于种内变异较大，不适于作为条码基因。研究结果为科学选用DNA条形码基因进行帘蛤目贝类的鉴定提供了参考资料。 Abstract:DNA barcoding has been widely used in the fields of taxonomy, identification, phylogenetic evolution, and population genetic analysis of marine shellfish. To further evaluate the identification validity of different DNA barcoding genes in marine shellfish, sequences of COI, 16S rRNA, 18S rRNA, and 28S rRNA from Veneroida were randomly downloaded from the GenBank database, analyzed by the distance-and tree-based methods, and taxonomic relation established by the tree-based method, and compared the results with some sequences from our laboratory. According to the "10×rule" and "2%" standard criterion, 57.1% of species could be distinguished using the COI gene, whereas 16S rRNA identified 60.9%, 18S rRNA identified 16.7%, and 28S rRNA did not identify any species. We also found that there were significant barcode gaps between the genetic distances of pairwise-and within-species in most genera based on COI and 16S rRNA genes; however, there were significant overlaps, instead of barcode gaps, based on 18S rRNA and 28S rRNA. The cluster analysis showed that 87.9% of individuals clustered to a monophyletic group, with other within-species individuals clustered on the COI; and 65.6% 16S rRNA individuals clustered in monophyletic groups. Furthermore individuals without monophyletic groups clustered into sister groups, which revealed that the evolutionary relationships showed by the NJ tree constructed via COI and 16S rRNA mainly agreed with that from the morphological classification, especially that of the COI gene. In contrast, the NJ tree constructed via 18S rRNA and 28S rRNA genes showed disordered clustering relationships, including some individuals from different species cluster to monophyletic groups. In the present study, the results clearly suggested that both COI and 16S rRNA can be used as DNA barcodes in identifying species in Veneroida, particular COI, but 18S rRNA and 28S rRNA are not suitable because of the large intraspecific variation. This study provides new data for DNA barcode selection in Veneroida. 参考文献 相似文献 引证文献

Mitochondrial DNA genetic diversity in the wild and two cultured Kaluga ( Huso dauricus ) populations in China

PDF HTML阅读 XML下载 导出引用 引用提醒 中国达氏鳇野生群体和两个养殖群体的线粒体遗传多样性分析 DOI: 作者: 作者单位: 1. 中国水产科学研究院黑龙江水产研究所, 黑龙江省冷水性鱼类种质资源及增养殖重点开放实验室, 黑龙江 哈尔滨 150070;2. 中国水产科学研究院黄海水产研究所, 山东 青岛 266071 作者简介: 户国(1984-),博士,副研究员,主要研究方向为分子数量遗传与水产动物育种.E-mail:huguo@126.com 通讯作者: 中图分类号: S917 基金项目: 国家科技重大专项子课题（2015ZX07201-008-05）；中国水产科学研究院基本科研业务费专项（2014A11）. Mitochondrial DNA genetic diversity in the wild and two cultured Kaluga (Huso dauricus) populations in China Author: Affiliation: 1. Heilongjiang Province Cold-Water Fish Germplasm Resources and Aquaculture Key Laboratory, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China;2. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:达氏鳇（基因和D-loop区域的多态性信息评估了黑龙江抚远段野生群体、北京房山国家级鲟鱼原种场的保种群体及浙江衢州国家级鲟鱼良种场的繁殖群体等3个达氏鳇群体的遗传多样性水平。所有测试个体在Cyt 单倍型，而在D-loop区域中发现了9种单倍型，对于D-loop区，单倍型多样性（）仅为0.00213。在8尾野生个体中检测到6种D-loop单倍型，2个养殖群体共计58尾个体共计检出5种D-loop单倍型个体。分析结果显示：野生达氏鳇群体遗传多样性极低，历史上可能经历过严重的遗传瓶颈，同时达氏鳇人工繁殖过程中每批次可能只有极少个体参与了繁殖。此外，基于Cyt Huso）的分类地位得不到有效的分子生物学数据支持。 Abstract:Kaluga () is the native sturgeon in the Amur river. Similar to other sturgeons, their populations have sharply decreased in recent decades; they have been identified as one of the most endangered species in the world. Unlike the rapid decline of wild populations, sturgeon farming has rapidly increased in China Mainland. The intra-specific hybridization of the female Kaluga and male Amur sturgeon () is widely performed, constituting a large part of sturgeon production in China. In this study, we use the Cyt gene and D-loop region of mitochondrial DNA to assess the genetic diversity of three Kaluga populations, including the wild population from the Amur river, and two populations cultured in national sturgeon breeding farms located in China:one from Fangshan, in Beijing city and the other from Quzhou, in the Zhejiang province. All of the tested individuals were of identical the Cyt haplotype, whereas 9 haplotypes were found in the D-loop region. There was no diversity detected at the nucleotide level on the Cyt d) reached 0.593, but nucleotide diversity () was only 0.00213. There were 6 D-loop haplotypes detected in 8 individuals from the wild population and 5 haplotypes detected in 58 individuals from the cultured populations. The results suggested the wild Kaluga population might have experienced a serious genetic bottleneck. Meanwhile, in the process of artificial propagation of Kaluga, only a few individuals might have been involved in reproduction each time in both the cultured populations. Phylogenetic tree analysis based on partial sequences of the Cyt genes supported the previous results from other researchers. The phylogenetic relationship between and other Pacific sturgeon species was close, but that between was not. Our result implied that the prevalence of the traditional genus was not supported by molecular genetic data. 参考文献 相似文献 引证文献

The Effects of Microalgae as Live Food for Brachionus plicatilis (Rotifer) in Intensive Culture System.

Brachionus plicatilis is used to feed fish and crustacean larvae in the aquaculture industry. It is well established that the type of microalgae may influence rotifer production. This experiment was conducted to determine the effect of five different locally available microalgae species at Fisheries Research Institute (FRI), Kampung Pulau Sayak, Kedah, Malaysia on the instantaneous growth rate (μ) of rotifer. Nannochloris sp., Tetraselmis sp., Isochrysis sp., Chlorella sp., and Nannochloropsis sp. were used as feed at different algae densities (0.1, 0.3, 0.7 and 1.5 × 106 cells/ml) and culture volumes (20, 70 and 210 ml). At algae densities ranging from 0.3 to 1.5 × 106 cells/ml, an average μ value of more than 0.90 per day were recorded for all algae species. However, at density of 0.1 × 106 cells/ml, only Tetraselmis sp. resulted in the significantly highest μ value compared with others (p < 0.05). In terms of volume, smaller culture volume of Tetraselmis sp. (20 ml) showed significantly higher μ compared with higher volume (70 and 210 ml cultures).