Aquarium Research Articles

Occurrence of Multi-Drug-Resistant Bacteria in Rainbow Trout–Lettuce Bio-Integrated Culture Systems in Chile

In an aquaponic system, fish and plants are cultivated together in a symbiotic environment where they mutually benefit, using significantly less water than traditional farming methods. The main aim of this study was to investigate the occurrence of antimicrobial resistance in two aquaponic systems implemented in two Chilean high schools using rainbow trout and lettuce cultures. When water samples (fish tank, biofilter, and plant raft) were analyzed over a three-month period, no resistance to oxytetracycline was detected, whereas the occurrence of resistance to florfenicol was rather small, ranging from 0.01% to 3.1% of bacterial culturable counts. Eighteen isolates were recovered from various sources as representatives of the florfenicol-resistant population, and all of them belonged to the Pseudomonas genus, showing a multi-drug-resistance phenotype and exhibiting simultaneous resistance to 7–13 antimicrobials. All isolates exhibited resistance to amoxicillin, chloramphenicol, florfenicol, and furazolidone and susceptibility to meropenem, oxytetracycline, oxolinic acid, flumequine, ciprofloxacin, and enrofloxacin. Five and two isolates carried the amphenicol-resistance-encoding genes floR and cmlA, respectively, whereas no carriage of integrons or the fexA, fexB, pexA, optrA, and cfr genes encoding for florfenicol resistance was detected. Eleven isolates carried plasmids, but only two of them were able to transfer their plasmid content by conjugation. The knowledge of the microbiome associated with aquaponic systems is still scarce, and their role as potential reservoirs of antimicrobial-resistant bacteria and related genes of these systems must be elucidated.

A Critical Assessment of the Process and Logic Behind Fish Production in Marine Recirculating Aquaculture Systems

A recirculating aquaculture system (RAS) represents a forward-looking form of aquaculture. A RAS consists of fish tanks and water treatment processes in a closed loop to sustain the environmental conditions for fish production. However, the rapid industrialization of the technology is fraught with transfer problems. This review justifies a RAS process chain based on fish biology. The underlying concept has been evaluated by the author in experimental and commercial RAS projects. The core idea is that the fish must be considered as a technical subcomponent in a RAS, determining the technology. Fish, when considered as small biological machines, are still a black box in many ways. However, their basic biology and physiology provide all the knowledge to implement them in a technical setting. The information required to understand this concept is presented and discussed based on current scientific knowledge. The conclusion is that the technology is available but needs to be rigorously implemented. If this were carried out, fish production in RASs would be ecologically sustainable, which is already claimed for RASs but is not always the reality in commercial applications.

Concept of a Cyber–Physical System for Control of a Self-Cleaning Aquaponic Unit

The article aims to present a cyber–physical system (CPS) to support the cultivation of aquaculture in a closed aquaponic system using the deep-water culture (DWC) method. The CPS uses precision sensors as TriOxmatic 700 IQ (for dissolved oxygen and water temperature), AmmoLyt Plus 700 IQ (for ammonium), NiCaVis 701 IQ NI (for nitrites and nitrates), SensoLyt® 700 IQ (for pH), and SL-M5 (for water level). It is built with a Raspberry Pi 4, 8 GB as a server, OpenHAB 3.0 software, and other specialized software for measuring water parameters. Some of the parameters are maintained completely autonomously, while others are indirectly controlled. Basic knowledge of hydroponics and aquaculture is required to set up the system, but day-to-day maintenance can be carried out by employees who receive instructions from the CPS. A method for the physical modification of the fish tank surface by using laser processing is proposed. This results in a change in surface topography (creating diverse microstructure patterns) and its roughness, which is of crucial importance for the bacterial adhesion mechanism.

Phenotypic and molecular detection of some Gram-negative bacteria from water of some commercial fish tanks in Mosul City/Iraq

Phenotypic and molecular detection of some Gram-negative bacteria from water of some commercial fish tanks in Mosul City/Iraq

In vivo imaging identified efficient antimicrobial treatment against Mycobacterium marinum infection in mouse footpads

Mycobacterium marinum (M. marinum) is the most common causative bacteria of cutaneous non-tuberculous mycobacterial (NTM) infections, including fish tank granuloma. Treating M. marinum-caused infection takes longer than other NTM diseases because M. marinum is less susceptible to antimicrobial agents. A standard treatment regimen for M. marinum infection has not been established yet, and few in vivo experiments have been performed in mammals to evaluate the bactericidal effects of antimicrobials. In this study, we developed a noninvasive in vivo imaging method to assess the therapeutic efficacy of antimicrobials against M. marinum infection. The data obtained using fluorescent protein or bioluminescence from luciferase will offer valuable insights into bacteria visualization across various bacterial infections. Furthermore, through this imaging technique, we demonstrated that combining clarithromycin, rifampicin, ethambutol, and minocycline effectively cleared M. marinum from the footpad. Granulomas with necrotic abscesses formed on the footpad of M. marinum-infected mice, primarily due to neutrophils involved in the host’s cell-mediated immune response. Inflammatory cytokine and chemokine levels significantly increased 7 days post-infection, aligning with the footpad swelling and granuloma formation observed in the untreated group. Interestingly, immune mediators and cells induced by M. marinum footpad infection were crucial factors associated with hypersensitivity and granuloma formation, as seen in pulmonary tuberculosis. This novel imaging analysis using a cutaneous NTM mouse model might be a powerful tool for the comprehensive analysis of mycobacterial infections.

Determination of the protein value of novel poultry meal ingredients for rainbow trout, Oncorhynchus mykiss

AbstractThe aim of this study was to assess the digestible protein value of three novel poultry products (Pea/Lentil‐PM, Hen on Hen‐PM, and soybean meal (SBM)‐PM), then a growth trial with a 2 × 5 design was conducted where SBM‐PM was included at five levels: 0, 5, 10, 15, and 30% in both a fish meal (FM)‐based diet and a plant protein (PP)‐based diet. Thirty rainbow trout (initial size = 10.7 ± 0.15 g; Troutlodge Inc, Sumner, WA) per tank were stocked into a 15°C recirculating system. Fish in triplicate tanks were fed twice daily to apparent satiation for 12 weeks. SBM‐PM had no effect on final weight (p = 0.3665; FM 233 vs. PP 237) or growth rate (p = 0.4703; FM 2070% vs. PP 2097%). Significant interactions between dietary protein and SBM‐PM for both intake (p = 0.0080) and FCR (p = 0.0081) indicate increased intake of fish fed the plant protein‐based diet without SBM‐PM. Regression modeling of this effect yields a polynomial model with an R‐square of 0.78; p < 0.0001 explained by the relationships: and , respectively. These values suggest that the SBM‐PM can be included up to 30% in rainbow trout feeds to reduce feed costs without negatively impacting performance.

Molecular Fish Sexing on Taisho Sanshoku Koi (Cyprinus carpio) Based on ArS.9-15 Gene Amplification using PCR Method

Taisho Sanshoku is a variant of Koi fish (Cyprinus carpio) that has high demand due to its high economic value and relatively expensive price. This study aimed to determine the sex of the Taisho Sanshoku Koi fish by molecular sexing using the PCR method to amplify the ArS.9-15 gene. This study was initiated by rearing a 4–6 month-old of 10 Taisho Sanshoku Koi fish in a fish tank with a filter and oxygen aeration. The fish were fed with fish pellets for 1–3 days. The Koi fishes were then anesthetized using Koi anesthesia containing β-hydroxyethyl phenyl ether. Each fish's peripheral blood was collected as much as 0.5 mL per fish and then stored in tubes containing Ca-EDTA anticoagulant. The genomic DNA was extracted from peripheral blood samples and used as a template DNA for PCR amplification targeting ArS.9-15 gene. Agarose with 1.5% concentration and CybrSafe staining was used in electrophoresis for visualization of the PCR results then visualized in a dark chamber using a UV transilluminator. The Taisho Sanshoku Koi fish's sex was determined using descriptive analysis based on the electrophoresis results. According to the PCR results, the female Taisho Sanshoku Koi fish only produced one 800 bp DNA band, whereas the male fish produced two 800 bp and 1,100 bp DNA bands. The outcome of molecular fish sexing of the 10 Taisho Sanshoku Koi fish reported that 60% were male and 40% were female.

AI-Driven Realtime Monitoring of Early Indicators for Ichthyophthirius multifiliis Infection of Rainbow Trout.

A novel video-based real-time system based on AI (artificial intelligence) was developed to detect clinical signs in fish exposed to pathogens. We selected a White Spot Disease model involving rainbow trout as the experimental animal and the parasitic ciliate Ichthyophthirius multifiliis as a pathogen. We compared two identical fish tank systems: one tank was infected by co-habitation, whereas the other tank was kept non-infected (sham infection). The two fish tanks were separately video monitored (full top and side view) during the course of infection, during which fish were removed whenever they developed clinical signs (direct visual inspection by the observer). Image analysis (object detection, classification and tracking) was used to track behavioural changes in fish (in every recorded video frame), focusing on movement patterns and spatial localisation. Initially, the two fish groups (infected and non-infected) exhibited similar behaviour and non-infected fish did not change behaviour during the 15 d observation period (from 5 d before infection until 10 dpi). At 4, 7, 8, 9 and 10 dpi some infected fish showed clinical signs (equilibrium disturbance, gasping and lethargy) and were removed from the experiment. Anorexia occurred from 5 dpi and a gradual progression of gasping behaviour was noted, whereas the frequency of fish flashing (rubbing/scratching against objects) was low. Equilibrium disturbances and the development of white spots in the skin appeared to be a much later (8-10 dpi at this temperature) indicator of infection. The video analysis showed a general distribution of non-infected fish in all parts of the fish tank during the entire experiment, whereas infected fish already at 4-5 dpi moved towards higher water currents in the top and bottom positions. This change of fish positioning within the tank appeared as a promising early indicator of infection. The study suggests that continuous monitoring of fish behaviour using AI can potentially optimise the timing of humane endpoints, indicate disease signs earlier and thereby improve animal welfare in both animal experimentation and in aquaculture settings.

Joint cultivation of fish and vegetable crops in an aquaponics system using floating beds

Comparative results of the cultivation of Silver crucian carp in conditions of aquarium circulation systems are presented, in one of which aquaponic cultivation of tomatoes and peppers on a floating bed in a fish tank was used. In comparison with the control, a significant improvement in hydrochemical parameters for dissolved oxygen and a group of nitrogen-containing compounds was noted in the experimental aquarium. The ratio of the specific formation as a result of the vital activity of fish and the absorption of nitrates by plants was 0,8 units. The results showed that for 1 kg of ichthyomass, 26 tomato plants or 34 bell pepper bushes can be planted on a floating bed. In the obtained fruits, the nitrate content turned out to be 2 times lower than the MPC for open and 4 times lower than the MPC for protected soil. The increase in the total ichthyomass of crucians in the experiment turned out to be 1,6 times higher, and feed costs 1,5 times lower than in the control. At the same time, the fish productivity of the aquarium with a floating vegetable garden increased by 9,7%.

Discarded blue mussel (Mytilus edulis): a feed ingredient that maintains growth performance of juvenile gilthead seabream (Sparus aurata) while fishmeal and fish oil are removed

ABSTRACT The total substitution of fishmeal (FM) and fish oil (FO) with sustainable feed ingredients, while maintaining acceptable growth performances and fish composition, is a challenge. Mussels have a high protein content, with essential amino acid and fatty acid (FA) profiles similar to those of FM. This study aimed to assess the potential to rear gilthead seabream (Sparus aurata) on a plant-based feed (without FM and FO) to which mussels were added. Subsequent effects on growth performance, protein content, and FA concentrations were studied. Juvenile gilthead seabream were reared for 46 days in water temperature of 21°C, in a recirculated aquaculture system composed of three fish tanks, each corresponding to one treatment: in the FMFO treatment, 342 fish were fed a commercial feed that contained FM and FO; in the plant-based feed (PBF) treatment, 345 fish were fed an experimental feed that contained only plant-based ingredients; in the mussel feed (MF) treatment, 342 fish were fed the feed from PBF supplemented with fresh mussel flesh from which the shells had been removed. Fish weighed a mean of 6.7 ± 1.3 g at stocking in each treatment. At harvest, the mean weight of fish from FMFO and MF were significantly different (23.1 ± 4.3 and 23.1 ± 5.2 g, respectively) than that from PBF (15.5 ± 3.6 g) (p < .05). Compared to the feed from FMFO, the feed from PBF decreased the percentages of eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and saturated FA (14:0, 16:0) in fish, but increased those of 18:1, 18:2 n-6 and 18:3 n-3 FA. Compared to the feed from PBF, the feed from MF increased the EPA+DHA concentration in fish by 21%. Thus, adding fresh mussels to a plant-based feed yielded similar growth performances and survival rate as a commercial feed. It also improved the FA composition of fish compared to that of fish fed a plant-based feed.

GROWING OF GREEN CORAL LETTUCE AND RED HYBRID TILAPIA IN COMPACT AQUAPONIC PROTOTYPE AT DIFFERENT RECIRCULATION RATES

In this work, a compact-size aquaponics prototype was designed and fabricated. One of the current challenges in compact aquaponics systems is optimizing the balance between fish waste production and plant nutrient uptake. The objective of the work is to assess the impact of different pump recirculation rates on the crops cultivation at such compact aquaponics unit. Main components included a 50 litres fish tank, a filter unit that combines the mechanical filter and biological filter counterparts, and nutrient film technique (NFT) grower. Production of food was carried out for 10 weeks at two different recirculation rates i.e. at 50 L/hr and 75L/hr. According to the data attained, the specific growth rate (SGR) was around 5.4-5.7% with a food conversion ratio (FCR) of about 1.8-2. Moreover, the fish survival rate was 80%, and plant growth yield is about 1.5-2 cm/week. Although the level of ammonia was slightly off the acceptable limit, no yellowish colour on the leaves of the green coral lettuce was observed. The data attained signify the successful operation of compact-size aquaponics for the production of organic leafy plants and freshwater aquaculture.

Smartphone region-wise image indoor localization using deep learning for indoor tourist attraction.

Smart indoor tourist attractions, such as smart museums and aquariums, require a significant investment in indoor localization devices. The use of Global Positioning Systems on smartphones is unsuitable for scenarios where dense materials such as concrete and metal blocks weaken GPS signals, which is most often the case in indoor tourist attractions. With the help of deep learning, indoor localization can be done region by region using smartphone images. This approach requires no investment in infrastructure and reduces the cost and time needed to turn museums and aquariums into smart museums or smart aquariums. In this paper, we propose using deep learning algorithms to classify locations based on smartphone camera images for indoor tourist attractions. We evaluate our proposal in a real-world scenario in Brazil. We extensively collect images from ten different smartphones to classify biome-themed fish tanks in the Pantanal Biopark, creating a new dataset of 3654 images. We tested seven state-of-the-art neural networks, three of them based on transformers. On average, we achieved a precision of about 90% and a recall and f-score of about 89%. The results show that the proposal is suitable for most indoor tourist attractions.

Streptomyces kunmingensis XK9 and galactooligosaccharide synergistically enhance growth performance, nonspecific immunity and disease resistance in striped catfish (Pangasianodon hypophthalmus)

Abstract The present study evaluated the effectiveness of the synergy of Streptomyces kunmingensis XK9 and galactooligosaccharides (GOS) in improving growth performance, nonspecific immunity, and protection of striped catfish (Pangasianodon hypophthalmus). Seven feed regimens were tested in striped catfish fingerlings, including commercial pellets supplemented with preparations S7, S8, and S9 containing XK9 at 107 CFU/mL, 108 CFU/mL, and 109 CFU/mL, respectively; G5 containing 5% GOS; S7G5, S8G5, and S9G5 including 5% GOS and XK9 at different doses of 107 CFU/mL, 108 CFU/mL, and 109 CFU/mL, respectively. After 3 months of treatment with different dietary regimens, weight gain, specific growth rate, weight gain rate, and feed conversion ratio were greatly improved in fish-fed diets containing S9, S8G5, and S9G5 (p&lt;0.05). Nonspecific immune parameters, including total white blood cell, phagocytic activity, and phagocytic index, increased consistently in fish that were fed diets containing synbiotics S8G5 and S9G5 (p&lt;0.05). Lysozyme and complement activity increased substantially in fish that were fed diets supplemented with S9G5 (p&lt;0.05). The protective effect of XK9 and GOS on striped catfish was evaluated after 15 days of challenge with Edwardsiella ictaluri. The results achieved show that the cumulative mortality rate sharply decreased in fish fed a diet containing the synbiotic S9G5, down 2.17 times compared to the control group; pathogen density was lowest in tanks of fish that were fed regimens containing S8G5, S9G5, and S9 (p&lt;0.05). The results suggest that synbiotics, including S. kunmingensis XK9 and GOS, have the potential to be applied in sustainable farming for the striped catfish industry.

Impact of the Color of Rearing Tank on Behavior and Growth of Nile Tilapia, Oreochromis niloticus (Linnaeus, 1758)

This study examined the impact of tank color (black, red, blue, green, and transparent) on the behavior (boldness and aggressiveness) and growth of Nile Tilapia over nine weeks (first, third, sixth, and ninth weeks). Boldness and aggression levels were assessed using open field and mirror image tests, respectively, for individual Nile Tilapia (Oreochromis niloticus). Healthy Nile Tilapia, initially weighing 2.51g on average, were used in the experimental trials conducted in tanks of different colors. The findings indicated that red tanks appeared to foster the highest level of boldness (p&lt;0.05), whereas fish in transparent tanks exhibited the highest level of aggressiveness compared to other tanks (p&lt;0.05). Moreover, individuals in black tanks displayed the highest growth rate in terms of weight (p&lt;0.05). This study suggests that Oreochromis niloticus can thrive better in black-rearing tanks, as the black tanks appear to enhance the growth rate of the fish, possibly through improvements in physiological processes.

IoT based Smart Aquaculture Monitoring System for Fish Tank Management

Aquaculture, the farming of aquatic organisms, plays a critical role in meeting the increasing global demand for seafood. Effective monitoring and management of aquaculture systems are essential for ensuring optimal conditions and maximizing productivity. This research presents a comprehensive aquaculture-based fish tank monitoring system designed to enhance operational efficiency and fish welfare. At the heart of the system is the Arduino Uno microcontroller, serving as the central processing unit to coordinate various functions. Key components include pH sensor for continuous monitoring of acidity levels, turbidity sensor for detecting suspended particles, and water level sensor for maintaining optical water levels within the tank. In the event of abnormal pH levels or high turbidity, the system triggers automated alerts, sending notifications to the designated owner through SMS and transmitting data to an IoT website for remote monitoring. To ensure consistent environmental conditions, two pump motors are utilized: one for automatic water replenishment in case of water level fluctuations and another for removing water in case of particle accumulation. Furthermore, the system integrates a servo motor mechanism for precise dispensing of fish feed at predetermined intervals, promoting healthy growth and nutrition. Control over pump motors and feeding schedules is facilitated through a user-friendly website interface, providing operators with seamless management capabilities.

Innovations in Aquaponics Technology and Building Sustainable Infrastructure for Agriculture

Aquaponics, a symbiotic integration of aquaculture and hydroponics, presents a promising approach to sustainable agriculture by maximizing resource utilization and minimizing environmental impact. This study explores recent innovations in aquaponics technology and the development of sustainable infrastructure to support its widespread adoption. A systematic literature review is conducted, showing the fundamentals of aquaponics and its key components such as fish tanks, grow beds, and biological filters. Innovations in technology are examined, including advanced monitoring and control systems, integration of renewable energy sources, automation, and novel growing techniques. Sustainable infrastructure for aquaponics is analyzed, focusing on water management strategies, energy efficiency measures, material selection, and integration with urban agriculture. Furthermore, the alignment of aquaponics technology and sustainable infrastructure development with the United Nations Sustainable Development Goals (SDGs) is studied, particularly in achieving goals related to zero hunger, clean water and sanitation, responsible consumption and production, and climate action.

Evaluation of productivity and efficiency of a large-scale coupled or decoupled aquaponic system

Aquaponics, a sustainable agricultural method, is gaining attention for its closed-loop system's potential to address diverse environmental and economic challenges in traditional agriculture. The aim of this study was to fill knowledge gaps in scaling up lab-scale systems to larger-scale decoupled aquaponics, offering a comprehensive understanding of the system's productivity and water and fertiliser use efficiency. In coupled aquaponics (CAP), the aquaculture, and the hydroponic units are arranged in a single loop, and water flows continuously from the fish tanks to the plant unit and vice-versa. In decoupled aquaponics (DCAP), the aquaculture and hydroponics units are arranged in a multi-loop setup as separate functional units that can be controlled independently. This work compared coupled aquaponics, decoupled aquaponics and hydroponics (HP, in perlite slabs) under the same climatic conditions with a substrate grown crop in a pilot scale greenhouse. The evaluation of growth parameters, yield, water, and fertiliser use for basil, cucumber, parsley, and tomato cultivations co-cultivated with the tilapia recirculating aquaculture system (RAS) are presented. The highest yields were achieved by DCAP plants in all experiments (15% higher than HP and 19% than CAP for basil, 38% than CAP in cucumber, 17% and 72% than HP and CAP in parsley, and 8% and 55% for HP and CAP in tomato experiments). The CAP treatment that did not receive any fertilisers presented the highest water use efficiency compared to both HP and DCAP treatments. Also, compared to HP, the DCAP treatment presented higher water and fertilisers use efficiency. Consequently, the adoption of decoupled aquaponic systems is the key to aquaponic evolution in the future.

Evaluation of a gel-based versus micro-pellet diet for adult zebrafish (Danio rerio).

Ad libitum feeding of laboratory zebrafish has potential benefits for colony management, but would require a new type of diet, such as a gel that remains in the tank. We hypothesized that adult zebrafish fed a gel diet would have similar body size and reproductive success compared with those fed a standard micro-pellet diet. The gel diet’s impact on water quality was determined to be safe for zebrafish prior to starting a 12-week feeding study. Two hundred adult AB zebrafish of mixed sex were randomly assigned to be fed exclusively either gel or micro-pellet diet. Fish body length and mass were measured every two weeks, and fish were bred within each feed group to assess fecundity. Zebrafish consumed less gel diet than expected. Body length, mass, and breeding success were lower in the gel diet fish than in the micro-pellet diet fish. Low consumption of the gel diet and/or nutritional differences between the two diets may have contributed to reduced growth and fecundity. Though the gel diet could reduce time personnel spend feeding and be safer for fish in static tanks, the tested formulation was not a satisfactory alternative to the control micro-pellet diet in a research zebrafish colony.

Where should I search next? Messages embedded in storybooks influence children's strategic exploration in Turkey and the United States.

Despite the vital role of curiosity-driven exploration in learning, our understanding of how to enhance children's curiosity remains limited. Here, we tested whether hearing a strategic curiosity story with curiosity-promoting themes (e.g., strategically approaching uncertainty, adapting flexibly to new information) versus a control story with traditional pedagogical themes (e.g., following rules, learning from others) would influence children's strategic exploration across two cultures. Three- to 6-year-olds from the United States (N = 138) and Turkey (N = 88) were randomly assigned to hear one of these stories over Zoom, before playing a game in which they searched for sea creatures across five fish tanks. All tanks had the same number of hiding spots but varied in the number of creatures they contained. Time was limited and children could not return to prior tanks, pushing them to allocate search effort strategically. Results indicated that across both countries, children in the strategic curiosity condition explored the virtual "aquarium" more broadly; they moved through tanks more rapidly than children in the control condition and were more likely to explore all five tanks before time ran out. Children in the strategic curiosity condition also showed relatively more strategic search, adapting their search based on the likelihood of finding creatures in each tank. While further research is needed to pinpoint which elements of our stories produced differences in search behavior and whether they did so by enhancing or inhibiting children's strategic exploration, storybooks appear to be a promising method for shaping children's exploration across multiple countries. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

Investigating the application of the aquaponic paradigm to Litopenaeus vannamei farming

Abstract Aquaponics, a sustainable farming system combining aquaculture and hydroponics, has been widely adopted, used, particularly in freshwater. This study explores the feasibility of adapting the aquaponic model to saltwater conditions, specifically for wastewater treatment from Litopenaeus vannamei farming using the salt-tolerant plant species Rhizophora apiculata Blume. It focuses on plant development and calculates the water treatment effectiveness in terms of nutrient removal in mg of NH4 +, NO2 −, NO3 −, and total phosphorus per gram of plant mass. The first phase involved seedling production, model creation, and testing the plant’s wastewater treatment capabilities. After three months of growth, the plants are subjected to shrimp wastewater, and their nutrient removal efficiency is determined. The obtained data provides essential parameters for the operation of the model during Phase 2. In Phase 1, R. apiculata Blume removed NH4 +, NO2 −, NO3 −, and total phosphorus at 95%, 97%, 59%, and 57% efficiency after seven days, respectively. It focuses on plant development and quantifies the efficiency of water treatment by measuring the removal of nutrients such as NH4 +, NO2 −, NO3 −, and total phosphorus per gram of plant mass.The calculated nutrient removal coefficients per gram of plant mass are NH4 +: 0.007, NO2 −: 0.005, NO3 −: 0.031 and total phosphorus: 0.121. In Phase 2, the designed aquaponic model was developed, which included a shrimp tank with 300 individuals per 1 m3, a fish tank with 50 individuals per 0.5 m3, and a plant growing system. The experimental aquaponic model demonstrates the technical feasibility of using R. apiculata Blume to recycle water in shrimp farming. The model shows potential for scaling up and offers additional benefits, as the leaves R. apiculata of can serve as an herbal source for aquatic species.