The present study was conducted to detect a correlation between Pb content in sediment and muscle of fish (Mystus gulio Ham. - Buch.) and downstream stretches (three study sites) of the of the river Hooghly, West Bengal, India. The study sites were selected at Batanagar (Bt), Budge Budge (Bg) and Birlapur (Br) sites. The samples viz. sediment and muscle of fish were collected and estimated by using atomic absorption spectrophotometer. The Pearson correlation coefficient analysis was performed to know the association between these sites and sediment as well as muscle during pre-monsoon season. The study sites observed both significant positive (P is less than 0.05) and negative correlation with sediment of river Hooghly at downstream while significant (Pis less than0.05) positive correlation was observed between the study sites and muscle of the fish specimen (Mystus gulio). The findings pose the risk of accumulation may be lower in fish muscle due to positive correlation, but negative correlation may lead to harmful impact in the study site for sediment. In future, this study may be beneficial to determine the water and sediment quality status in different stations of river Hooghly as well as other metals content in different fish species.