This study evaluates the effectiveness of a 200-kHz inverted echo sounder for monitoring the abundance and behavior of near-surface zooplankton and fish. Data from both oceanic and littoral environments are examined: first from an 81-day deployment at Ocean Station Papa (OSP) in the northeast Pacific Ocean during the spring of 1996, and second from an 8-day deployment in the southern Strait of Georgia in September 1998. The analysis combines calibrated backscatter intensity, echo-amplitude statistics, and acoustic-scattering models to produce estimates of mean scatterer size and abundance. The identity of the various scatterer classes is deduced from local net trawls and reference to previous studies. At the OSP site the dominant scatterers were found to be euphausiids, pteropods, and myctophid fishes, with mean lengths of 15, 1.5, and 28 mm, respectively. At the Strait of Georgia site three fish size classes were identified: juvenile herring with mean length near 10 cm, juvenile salmon with mean length of 20 cm, and there was weak evidence for an adult salmon class. Overall, the acoustically derived abundance estimates were in reasonable agreement with the local net trawls and results from previous studies. The usefulness of sustained monitoring over diurnal and seasonal time scales is demonstrated with the OSP zooplankton data.