Fish Production Research Articles

Inactivation of Anisakis simplex Allergens in Fish Viscera by Acid Autolysis

The evisceration of infested species on board commercial fleets and the throwing of viscera into the sea, as is mostly the case for hake, is common practice for reducing the occurrence of Anisakis in fishery products. Moreover, the high levels of infestation and the lack of technically and economically feasible solutions hinder the possibility of recovering viscera with other fish processing byproducts as raw material for feed without the risk of transmission of allergens to humans, with the subsequent risk for public health. The aim of this work was to study the fate of Anisakis simplex allergens during 11 days of silage of infested hake (Merluccius merluccius) viscera, as a potential method for eliminating this risk. While the viscera were almost completely liquefied, an increase in the allergenicity of the soluble fraction was observed, which decreased only slightly after day 9. As we are aware of the resistance of parasite allergens to gastrointestinal enzymes, silage was also analyzed after 12 and 15 months. While the lower molecular weight fragments of Anisakis proteins are fully digested, some larger fragments with potential allergenicity resisted autolysis after long silage periods, but they were present in a very low concentration. The study concludes that there is the potential of silage as a method for recovering fish viscera infested with Anisakis.

A Global Synthesis of Environmental Enrichment Effect on Fish Stress

ABSTRACTThe stress‐coping ability (SCA) is one of the core aspects of fish welfare and is of vital importance for fish production in the aquaculture industry and for fish fitness in hatchery release. Environmental enrichment (EE), a method of introducing external stimuli into the husbandry environment, has been recently proposed to improve fish SCA, but the present experimental evidence is mixed, and the reasons for these discrepancies are unclear. Here, we conducted a global meta‐analysis using a data set that consists of 1786 cases from 107 studies across 42 fish species to solve this problem. Overall, we found that enriched fish had significantly higher SCA than control fish, reflected in either basal stress levels or stress responses. Meta‐regression analyses showed that specific subgroups of enrichment type, fish developmental stage, stress category, stress duration, stress place, sample tissue and indicator system showed significant positive EE effects on SCA. Multi‐model inference indicated that the indicator system, fish developmental stage, stress characteristic and enrichment mode are important drivers for the high heterogeneity among effect sizes. These results highlight the importance of introducing EE into the rearing systems, which will not only increase the welfare of aquaculture fish but also improve the ecological adaptability of released fish. The comprehensive knowledge obtained in this analysis will provide insights into fish ontogenetic plasticity and its responses to EE and have important implications for improving the production cycle in fish aquaculture and fisheries conservation.

The role of the Mediterranean Diet in preventing the development and complications of lifestyle diseases – a Review

IntroductionThe Mediterranean diet, inspired by traditional dietary habits of the Mediterranean region, exhibits anti-inflammatory and protective effects, reducing the risk of civilization diseases such as obesity, type 2 diabetes, and cardiovascular disorders. This diet is characterized by a high intake of plant-based foods, olive oil, and moderate consumption of fish and dairy products. These components provide unsaturated fatty acids, polyphenols, and antioxidants, which positively impact metabolic health and lower the risk of chronic diseases. In the face of the global obesity epidemic and an increasing number of individuals affected by lifestyle-related diseases, the Mediterranean diet offers a promising tool to support a healthy lifestyle. Aim of study This article aims to review current scientific studies evaluating the potential of the Mediterranean diet in preventing and managing civilization diseases. Particular attention is given to its effects on weight loss, improvements in glycemic markers, reductions in low density lipoproteins (LDL) cholesterol levels, and blood pressure control. The article also explores its benefits for athletes' health, as well as its potential to reduce oxidative stress and inflammatory states. Material and methods A literature review of the PubMed database was conducted, assessing relevant studies on the Mediterranean diet and its impact on lifestyle diseases.

Pesticide Residues in Cabbage and Nile Tilapia and Implications on Human Health and Ecosystems: A Case of Fogera District in Ethiopia.

Pesticides have posed health risks to consumers and the ecosystems in different parts of the world, including Ethiopia, and researchers recommend continual assessments of pesticide residues in food items and ecosystems to know the level of risks. This study aimed to quantify the pesticide residues in samples of cabbage and fish and their risks to humans and the ecosystems. The cabbage samples were collected from April to May 2023 from 3 market centers, and the fish samples were collected in June 2023 from two fish ponds of Fogera District of Ethiopia using appropriate sampling procedures, extracted using the modified QuEChERS methods, and analyzed using a triple quadrupole GC/MS technique to quantify the pesticide residues and level the risks to humans and the ecosystems. The findings of the present study confirmed that all the samples of cabbage and fish were contaminated with pesticide residues. More than 44% and 37% of pesticide residues detected in both cabbage and fish samples were organochlorine and organophosphate pesticides, respectively. Furthermore, 92.86% of the pesticide residues and 96.43% of the maximum pesticide residues in cabbage exceeded the MRL and the TQ set by the Codex Alimentarius Commission. The sum total hazard indices of the pesticide residues in the cabbage (22.320) and fish (43.071) were much higher than the threshold value. Though Fogera District is a potential area for fish production in ponds, agricultural pesticides are a threat to the sector. Establishing an efficient system of monitoring for the pesticide supply chain and application procedures, choosing the appropriate pesticide types, timing in spraying, and establishing pesticide-free buffer zones are crucial steps in mitigating the negative effects of pesticides in the area.

Climate Change Affects Multiple Coral Reef Fisheries Ecosystem Services

ABSTRACTCoral reef fisheries support livelihoods and provide an affordable source of essential dietary nutrients to coastal people. However, climate‐driven coral bleaching is dramatically altering reef habitats and changing reef fish composition, diversity, and productivity. We used data from fisheries independent surveys and artisanal trap landings to explore how reefs with different responses to coral bleaching differed in habitat configurations, fisheries catch rates, economic returns, and nutritional content. Coral‐associated fishes supported the highest catch rates, greatest species diversity, highest rates of economic returns for fishers, and a wide price range for consumers. Macroalgae‐associated fishes supported the greatest overall fisheries yields, which indicated higher fishery dependence on these habitats. Nutrient content was high in catches across all habitats, but concentrations varied by nutrient, which suggested that fishing across a range of reef habitats should provide the greatest variety of nutrients to coastal communities.

Analyzing the adoption of nutrition-sensitive carp-SIS polyculture technology: evidence from a case study in Bangladesh

IntroductionSmall indigenous species (SIS) of fish are rich in micronutrients that are essential to combat the existing malnutrition in Bangladesh. However, their availability is constantly decreasing due to gradual environmental degradation making their availability irregular and hence expensive in the market. Integrating SIS with carps in homestead ponds is being promoted as a form of nutrition-sensitive aquaculture to enhance both production and consumption of these nutrient-rich species. Various improved pond management techniques (IPMTs) are suggested to boost the nutrition-sensitive carp-SIS polyculture.ObjectivesThis study examines the trends and factors influencing the adoption of IPMTs using the sustainable livelihood framework (SLF) approach. We address the following three key questions: (i) What are the trends in production and consumption of fish, in particular SIS from homestead ponds? (ii) Have farm households adopted or disadopted IPMTs over time? (iii) What are the determinants of adoption and disadoption of IPMTs?MethodsBased on primary data of 234 households from Barishal district from 2014 and 2022, we perform significance tests to compare project and non-project households in 2014 versus 2022 and apply fixed effects Poisson regression and fixed effects negative binomial regression models to identify household decisions to adopt the IPMTs.Results and conclusionQuantitative survey results indicate a significant increase in the production and consumption of SIS over time. In 2014, the project households exhibited adoption rates of 60% or more for various IPMTs such as stocking of fast-growing species, pre-stocking liming, pond dike construction, fertilizer application, stocking of high quality fish seeds, aquatic weed control, and turbidity management. However, by 2022, some of these IPMTs were disadopted by project households and only a few techniques, including providing sunlight exposure to ponds, pre-stocking liming, and supplementary feeding, were more widely used. Additionally, many non-project households also increased their adoption rates of IPMTs significantly. The fixed effects regression model shows that adoption is positively influenced by the number of household members participating in aquaculture, size of the pond, sole ownership of the pond and the number of years household has been involved in aquaculture. Follow-up activities such as monitoring and training of the project beneficiaries and government support are recommended to support long-term adoption of the IPMTs.

Investigating the Presence of Selenoneine, Ergothioneine, and Selenium-Containing Biomolecules in Fish and Fish-Derived Commercial Products.

Two potent antioxidants, selenoneine and ergothioneine, have been determined simultaneously for the first time in the muscle of highly consumed fish and in fish-derived products. The extraction methods were optimized, and their subsequent analysis was performed by HPLC-ESI-MS/MS. Best extraction efficiency (106 ± 3 and 104 ± 5%) was attained by performing a simple aqueous extraction containing 100 mmol L-1 ammonium acetate or ammonium formate as extractants. Selenoneine and ergothioneine were simultaneously detected in tuna (0.306 ± 0.006 and 3.5 ± 0.2 μg g-1) and swordfish (0.14 ± 0.02 and 1.1 ± 0.4 μg g-1) muscle tissues, while in fish roe and in farmed salmon, only ergothioneine was detected (with contents ranging 0.03-3.3 μg g-1). Moreover, the presence of their respective methylated forms was also considered. Additionally, Se-containing biomolecule profiles from water-soluble proteins were obtained by SEC-ICP-MS analysis. Results revealed similar profiles between fish and fish roe, whereas for ultraprocessed fish products, Se seems to be mostly associated with low-molecular-weight biomolecules. The results included in this work may contribute to a better understanding of the relations between selenoneine and ergothioneine in fishery products as well as their fate and potential modifications of Se-containing biomolecule profiles during fish processing.

Evaluating Growth Performance and Economic Efficiency of Indian Major Carps in Integrated Fish-duck Farming Systems

The experiment was conducted in three villages in Namakkal district to evaluate the effects of an integrated fish-duck farming system on the physico-chemical and biological parameters of pond ecosystems, as well as on overall fish production. This eight-month trial took place from July 2016 to February 2017. Ponds were stocked with fingerlings at a density of 6,000 per hectare, using Indian major carps like Catla (Catla catla), Rohu (Labeo rohita), and Mrigala (Cirrhinus mrigala) in a 4:3:3 ratio to maximize energy utilization through polyculture. Indian Runner ducks (Anas platyrhynchos) were included in the system to provide meat and naturally fertilize the ponds through excreta while grazing freely. In this village-based cultural practice, fish were not given supplementary feed. Instead, the ducks were fed with household food scraps and agricultural by-products, such as broken cereal grains and rice bran, which are readily available in rural areas. The study revealed that water quality parameters such as pH, dissolved oxygen, and alkalinity were significantly higher in the integrated ponds compared to control ponds (without ducks). Additionally, plankton levels (both phytoplankton and zooplankton) improved markedly in the integrated ponds. The Indian major carps in the integrated ponds also achieved better body weight than those in the control ponds. This improved growth rate contributed to a yield of 8,928 kg/ha/year in the integrated system, compared to 5,145 kg/ha/year in the control ponds. These results demonstrate that integrated fish-duck farming is more profitable than non-integrated systems (control) under rural conditions in Tamil Nadu, as it yields higher fish production without additional inputs.

Economic Impact of Periphyton-Based Stunted Fingerling Production in Arunachal Pradesh, India

Non availability of right type quality seed at right time in required quantity is major bottleneck for the growth of aquaculture in Arunachal and major portion of seed are being import from other nearby state especially Assam in particular. To make availability of good quality seed at right time having a regional acclimation for a longer duration in far flung areas of the state, the present investigation was conducted on low cost Periphyton (grown on paddy straw) based stunted yearling production technology to improve the survival and health of stocked material and their economic impact on the fish farmers of district West Kameng during 2018-2021. The average values of pond water quality parameters were observed to vary between both the culture ponds with a significant seasonal differences at all the sites and there were found a greater difference in average survival rate of both the commodities i.e. table size fish as well as stunted yearling during whole culture period. The involved cost of cultivation was recorded higher Rs. 4,70,000 / ha in stunted yearling culture in comparison to Rs.424,940/ha in thetable size carp culture while average gross benefit was just opposite, recorded higher Rs. 6,90,000/ ha in stunted yearling culture in comparison to Rs.199,960/ ha in table size carp culture with the same trend in BCR i.e. 2.46 for stunted fingerling production and 1.47 in the production of table size fishes respectively.

Common Carp (Cyprinus carpio) Fish Aquaculture Revitalization, Multiplication and Sustainability in Nigeria

Thirty three mature broodfish of Common Carp were harvested from Panyam Fish Farm, Plateau State (PFFPS), and from Federal University of Agriculture Makurdi Research Fish Farm (FUAMRFF). These farms are situated in two different agro-ecological zones of Nigeria (with a fading history of Carp fish culture). The broodfish were fed for 90 days, in preparation for on-farm breeding activities with the aim of determining water quality, hatching parameters (through un-induced natural spawning, induced natural spawning and induced breeding by stripping in indoor concrete ponds); thirdly, to determine percentage survival of the bred Carp fish progenies cultured for 56 days (in ponds sampled in PFFPS and FUAMRFF). Fourthly, to evaluate growth and potential economic returns from the two areas, and finally to further evaluate fish farmers perception of Carp fish culture in selected fish farms across other agro-ecological zones in Nigeria. After breeding activities, progenies were cultured and fed to satiation. The water quality parameters in the ponds and in water holding tanks was monitored daily. At the end of the research period, PFFPS showed better Carp fish hatching tendency, highest (94%) in Induced Natural spawning), with better environmental condition and water physico-chemical parameters for Carp fish culture with mean Dissolved Oxygen content level (8.35 Mg/l), and pH (7.54). Furthermore, PFFPS has capacity to accommodate the mass production of Carp fish. Economically, PFFPS had a higher gross margin (402,250), and Profit index (5.0). Also, result from fish farmers perception on carp fish culture showed that 62% of farmers have no idea of Carp fish management, 66% have no idea of Carp fish selling price, 66% are aware of PFFPS and 100% expressed that more awareness should be created on aquaculture diversification (with interest in Carp fish aquaclture), as this will meet consumer demand and rescue this species from its current track of going extinct in Nigeria, if given urgent attention).

Abstract 4135338: Associations between plant-based diets and total mortality among Chinese older participants with cardiometabolic diseases: a prospective cohort study

Background and Aims: Plant-based diets have been associated with a lower risk of total mortality among generally health populations. However, the associations of plant-based diets with total mortality remain unclear among older people with cardiometabolic diseases, who have an elevated risk of mortality. Methods: We prospectively followed 4,485 participants (mean age: 84 years) from the China Prospective Longitudinal Survey of Health and Longevity (2006-2018), who had cardiometabolic disease in 2006. Dietary data were collected at baseline using a food-frequency questionnaire. Using these data, we created an overall plant-based diet index (PDI), in which plant-based foods received positive scores while animal foods (meat, fish and aquatic products, eggs, milk, animal fats) received reversed scores accordingly. We also created a healthful plant-based diet index (hPDI), where healthy plant-based foods (whole grains, vegetables, vegetable oils, fresh fruits, legumes, beans, garlic, nuts, tea) received positive scores, while less healthy plant-based foods (refined grains, pickles, sugar, preserved vegetables) and animal-based foods were given reversed scores. In addition, an unhealthful plant-based diet index (uPDI) was created by assigning positive scores to less healthy plant-based foods while reverse scores to healthy plant-based foods and animal-based foods. We used Cox proportional hazards regression to compute hazard ratios (HRs) and 95% CIs for total mortality, with adjustment for demographics, dietary and lifestyle factors, and medical history. Results: During a 12 years of follow-up, 2,881 deaths were recorded. A higher score from PDI and hPDI was associated with a lower risk total mortality among participants with cardiometabolic diseases (HR and 95% CI for extreme quintiles of PDIs: 0.85, 95% CI 0.75–0.96, P trend < 0.001; hPDI: 0.84 (0.74–0.94), P trend < 0.001). A higher uPDI score was associated with a higher total mortality among participants with cardiometabolic diseases (uPDI: 1.28 (1.13-1.47), P trend < 0.001). These results were consistent in subgroup analyses by age, sex, smoking status, alcohol consumption, education level, physical activity, and BMI. Conclusion: Our study suggests that among older Chinese participants with cardiometabolic diseases, adherence to an overall plant-based diet or a healthful plant-based diet, is associated with a lower total mortality, while an unhealthful plant-based diet was associated with a higher total mortality.

PROFITABILITY ANALYSIS OF CATFISH PRODUCTION IN KANO METROPOLIS, KANO STATE, NIGERIA

Fish is an important dietary element and a major source of animal protein in human diet for many Nigerians. The demand for fish has been on the increase because of geometrical increase in human population, this has resulted to a significant gap created between fish demand and supply. It is obvious given the importance of fish production that efforts must be made to encourage entrepreneurs to go into the business. However, many have shown doubts on the productivity and profitability of fish production, particularly on a smallholder level. This study therefore, examines the profitability of catfish production in Kano metropolis, Nigeria. Two stage sampling procedure was employed to select respondents for the study. Primary data were collected with the use of well-structured questionnaire administered to 120 catfish producers. The tools of analysis employed were descriptive statistics, gross margin and net farm income. The study revealed that Majority of the producers (69%) are male. Furthermore, 85percent of the fish producers have attained post primary education. The gross margin (GM) was ₦657,420, while the enterprise profit which is the net farm income was estimated at ₦598,128 and 0.33 return to capital for the farmers. Some of the challenges confronting the respondents in their business includes high cost of feed, lack of government support and lack of training. Thus, there is a need for provision of training for higher level of productivity, subsidizing of inputs such as feed by the government and participation in cooperative societies by the fish farmers for financial and inputs assistance by relevant Non-Governmental Organizations.

Efektivitas Penyelenggaraan Tempat Pelelangan Ikan (TPI) Desa Sungaibuntu Kecamatan Pedes Kabupaten Karawang Jawa Barat

The fish auction serves as a marketing center for the distribution of fish. The implementation of the fish auction is expected to enhance the selling prices of the fishermen's catch. The purpose of this research is to analyze the level of effectiveness of the fish auction implementation. Effectiveness is measured in two ways: first, effectiveness is measured using the Mahmudi formula. Variables measured include the number of boats landing fish at the fish auction, fish production, fish prices, revenue, auction fees, fish auction operational activities, and the reporting system at Sungaibuntu’s fish auction. Second, effectiveness is measured according to the fishermen's perceptions, with variables including fish auction as a facility, auction activities, and services. This research also analyzes the comparison of fish prices and the income of fishermen selling fish at the fish auction and outside the fish auction. This research uses descriptive quantitative methods. Primary data collection involves field observations, questionnaires, and interviews, while secondary data is obtained from relevant institutions, theses, and journals. Simple random sampling is used to obtain 37 fishermen respondents. The research results show that the overall effectiveness ratio values measured by the Mahmudi’s formula for the period 2020-2022, for the effectiveness of boat activities, auction fees, fish production, and auction revenue, are categorized as "Less Effective." Meanwhile, the effectiveness of TPI operational activities and monitoring reporting systems achieves a level of "Very Effective," and the effectiveness of fish prices at Sungaibuntu TPI reaches the level of "Effective." For effectiveness measured according to the fishermen's perceptions, the variables of fish auction as a service and facility are categorized as "Less Effective." Meanwhile, fish auction as a facility is categorized as "Effective." There is a difference in the income of fishermen selling fish at the fish auction compared to fishermen selling fish outside the fish auction.

Growth Patterns of Small Pelagic Fish in West Africa

Small pelagic fishes play important ecological roles in marine ecosystems, constitute some of the most economically valuable fisheries resources, and play a vital role in West African food security. Often living in upwelling regions, these species appear to have developed mechanisms to cope with environmental variability, such as opportunistic reproductive tactics, enhancing their growth performance, or increasing their egg production by following the more predictable system attributes of seasonal cycles. To test this hypothesis, we investigated size-dependent patterns of the two growth mechanisms (i.e., growth rate) of two West African small pelagic populations (Ethmalosa fimbriata and Sardinella maderensis) in upwelling environments. These results were discussed with other areas in African tropical Atlantic waters. The monthly mean length of both species showed a large variation over the study period. Based on the fish length-frequency data and a coastal upwelling index, we found that the growth peaks of the species tended to occur during the most intense periods of upwelling (March–April). This study showed a significant decrease in size compared with other species found in other regions. It demonstrates how the geographical distribution of the same species, together with location-specific variation in temperature and food, can combine to determine local and regional growth responses in pelagic fish. Changes in growth rate may be an adaptive tactic in response to environmental change, as well as phenotypic plasticity in fish. This knowledge is essential to predict future changes in fish productivity and distribution vs. climate and to provide effective advice for ecosystem-based management.

The impact of quality certificates on sustainable food production: An analysis of selected systems in Poland

This study evaluates the influence of quality certificates on sustainable food production in Poland, considering economic, social, and environmental dimensions. Analyzing 25 different certificates, the research explores their criteria, procedures, and costs across various food product categories, including meat, fish, and plant-based products. The study provides a detailed review of certification processes, from initiation to audits and inspections. It identifies both commonalities and differences among certificates, each addressing unique aspects such as environmental impact, worker rights, and product origins. Despite the diversity in standards and procedures, the study underscores the need for standardized international criteria to improve transparency and meet consumer expectations, highlighting the significant role of quality certificates in advancing sustainable food production.

Prospects for improving the use of fixed capital in the fishery complex (the case of Primorsky territory)

One of the tools for the stable functioning of organizations is to increase the efficiency of the use of fixed capital. The main directions of development of the fishery complex are determined by the corresponding state program approved by the resolution of the Primorsky Territory Administration. One of the tasks is technical re-equipment, stimulating the renewal and modernization of fixed production assets, which should lead to an increase in the output of fish products. In accordance with the program, positive changes are already taking place in the industry. However, there are still unresolved problems regarding the efficiency of the use of fixed capital and its improvement in the future. In the article, the factors that determine the prospects for increasing the efficiency of the use of fixed capital of fishery organizations are analyzed using quantitative assessments.

Valorizing sardine scales: a circular approach to sustainable collagen for cosmetics and nutrition applications

Background and ObjectiveIn recent years, the consumption of fish products has led to a worrying trend where approximately two-thirds of the total amount of fish is discarded as waste. At the same time, scientific interest in exploring natural collagen sources for cosmetics and dietary supplements has increased. This study explores the potential of valorizing sardine scales (Sardina pilchardus), a by-product of the canning industry, through the extraction of collagen for potential use in dermocosmetic formulations and food supplements.MethodsCollagen from sardine scales was obtained though acid and enzymatic extraction. The collagen extracts were characterized by UV-Vis, FTIR spectroscopy, SDS-PAGE, powder X-ray diffraction (PXRD) and scanning electron microscopy (SEM). The collagen was hydrolysed with papain to small peptides. Subsequently, the biological activities of acid-soluble collagen as well as the collagen peptides in terms of antioxidant and antimicrobial activity were evaluated. Furthermore, the capacity of collagen peptides to permeate the intestinal barrier, simulated with caco-2 cells, was evaluated.ResultsPurified collagen extracts were obtained from sardine scales, with enzymatic extraction method having a yield three times higher than the acid method. The SDS-PAGE analysis confirmed the extraction of type I collagen as well as its hydrolysis into small fragments (25–12 kDa). In terms of biological activities, collagen and collagen peptides have not demonstrated antimicrobial activity. However, regarding antioxidant activity, collagen peptides showed three times more capacity compared to non-hydrolyzed collagen. Meanwhile, in 6 h, about 6.37% of collagen peptides could permeate the intestinal barrier.ConclusionThis work represents a continuous effort to advance our understanding and utilization of Portuguese marine waste resources, with focus on the valorization of sardine co-products for the development of food supplement or cosmetic formulations, contributing to the sustainable evolution of the circular blue economy.

Smoked Fish Salam: Development and Sensory Analysis

Objective: The aim of this study is to develop smoked salamis (cold and hot) from mechanically separated meat (MSM) of tilapia, and to evaluate their acceptance through sensory analysis. Theoretical Framework: With the increase in fish production, large quantities of fish by-products are also generated, such as carcasses, heads, fillets, among others with high nutritional value that can be used in human and animal nutrition. Method: The methodology adopted for this research includes a control formulation subjected to smoking (cold and hot), and two others with the inclusion of 10 and 20% of CMS in natura, totaling four treatments. In the dehydration process, the salamis were weighed and subjected to pre-drying in a forced ventilation oven for three hours at 55ºC. Then, they were placed in a stainless steel smoker that produced smoke by burning sawdust (4h/55ºC). The cold smoking process was carried out using 20% of liquid smoke/volume of water, where the salamis were immersed for 15 minutes, drained and taken to the oven (4h/55ºC). At the end of the process, the weight loss of the sausages was calculated and sensory analyses were performed, where 50 untrained judges evaluated the products in terms of acceptance and purchase intention. Results and Discussion: The results obtained revealed that total losses ranged from 16.71 to 32.79%. The type of smoking influenced the acceptance of the salamis (p<0.05), where color and aroma were the parameters that presented the highest values for hot-smoked salamis, as observed for purchase intention. Research Implications: Fish salamis enriched with fish protein sources are an excellent alternative for human nutrition, as they represent an excellent nutritional source for people who are concerned about healthy foods. There are several studies using animal protein sources such as fish to prepare healthy and easy-to-prepare foods, since due to people's fast-paced routines today, they need quick and healthy foods. Originality/value: this study contributes to the literature by highlighting the supplementation of food with processed fish by-products and enhanced in the form of fish sausages.

Bioaccumulation and tissue distribution of pharmaceuticals and their transformation products in fish along the pollution gradients of a wastewater-impacted river

A field study on the occurrence and distribution of forty-three pharmaceutically active compounds (PhACs) in water and fish samples from anthropogenically impacted section of the Sava River (Croatia) was performed to estimate the importance of bioaccumulation for the environmental risk assessment of PhACs. The study was performed using a highly specific LC-MS/MS method, tailored to include the most prominent PhACs from different therapeutic categories as well as their major metabolites and/or transformation products (TPs). The results revealed a widespread occurrence of PhAC residues both in water and fish samples with a large spatial variability reflecting the distance from the dominant wastewater discharges. The most prominent PhAC categories in less polluted upstream part of the river were common psychostimulants caffeine and cotinine, therapeutic opioids and cardiovascular drugs, while in the river section affected by the local municipal and industrial wastewater inputs, antibiotic drugs became clearly predominant, especially in fish tissue samples. The apparent bioconcentration factors (BCFs) of investigated PhACs varied over several orders of magnitude, from 0.02 ± 0.01 Lkg−1 for O-desmethyl tramadol in fish muscle to 784 ± 260 Lkg−1 for terbinafine in fish liver, indicating rather large differences in their bioconcentration potential and affinity to different tissues, with the tissue-specific BCFs increasing in the following order: muscle < gills < gonads < heart < liver < kidneys. The bioconcentration potential of most of the PhACs included in this study was only low to moderate however moderately high BCFs of certain PhACs (e.g. sertraline, terbinafine, loratadine, diazepam and azithromycin) in some tissues should be taken into consideration when assessing their potential environmental risks. Moreover, it was shown that BCFs could be strongly affected by biotransformation in fish. Risk prioritization based on risk quotient (RQ) and ToxPi index, revealed antibiotics, in particular azithromycin, and therapeutic psychoactive substances as the most hazardous pharmaceutical contaminants in the Sava River.

Cellulose-based halochromic sensor for real-time surveillance of spoilage of packed fish

The development of a biopolymer-based color-changing visual sensor for monitoring the spoilage of fishery products is described in this study. At first, a cellulose-based composite film containing mixed-indicator dyes was fabricated. The film was characterized using UV–visible and FT-IR spectroscopy, scanning electron microscopy, thermogravimetric analysis, lab, and RGB measurements. The halochromic properties of the film were evaluated through ammonia test and colorimetry. The developed film sensor is tested for monitoring the spoilage of packed seer fish fillets at room temperature for up to 48 h and at 4 °C for up to 10 days. The sensor displayed distinct color changes correlating with the spoilage of the packed fish. Analysis of total volatile basic nitrogen and pH levels of the samples revealed a correlation between the halochromism of the sensor and the spoilage of the fish. The color-changing pattern of the sensor was consistent with the results of microbial analysis. The present study reveals a promising material combination for fabricating simple visual sensors from biomaterials, which can be used to monitor the real-time deterioration of packaged fishery products.