Replacement Of Fish Meal In Diets Research Articles

Application prospect of replacement of fish meal with spray-dried egg meal in diets for swimming crab (Portunus trituberculatus)

An 8-week feeding trial was conducted to evaluate the effects of replacing fish meal with spray-dried egg meal (SEM) on growth performance, anti-oxidative capacity, mTOR pathway, and cholesterol metabolism in juvenile Portunus trituberculatus. The results showed that the highest percent weight gain was observed in crabs fed diet with 10% replacement of fish meal with SEM. Essential amino acids in the muscle and hepatopancreas significantly decreased with the increase of dietary replacement levels of fish meal with SEM. The lowest content of MDA in hepatopancreas was occurred at those fed diet with 40% replacement of fish meal with SEM, the activities of T-AOC, GSH-PX and content of GSH in hepatopancreas significantly increased with dietary SEM replacement fish meal increasing from 10% to 50%. The expression of akt, eif4e1a, eif4e2, eif4e3, and 4ebp1 significantly up-regulated, however, the expression of tor was showed significantly linear down-regulated, and the highest expression of akt, eif4e2 and 4ebp1 were found in crabs fed diet with 20% replacement of fish meal with SEM, the highest expression of eif4e1a was observed in those fed diet with 10% replacement of fish meal with SEM, the highest expression of eif4e3 was occurred in crabs fed diet with 30% replacement of fish meal with SEM. In cholesterol metabolism, the expression of abcg1 and lrp2 significantly down-regulated and the expression of npc1 significantly up-regulated with increase of replacement level of fish meal with SEM. The content of T-CHO in hemolymph and hepatopancreas significantly linear increased. In conclusion, based on quadratic broken-line regression analysis of PWG against the dietary replacement of fish meal with SEM, the optimal replacement level of fish meal with SEM was estimated to be 15.94% for juvenile swimming crab.

Chemical and Physical Properties of African Catfish (Clarias gariepinus) Fillet Following Prolonged Feeding with Insect Meal-Based Diets.

A 25-week experiment was undertaken to explore the effect of partial replacement of dietary fishmeal (FM) with black soldier fly meal (Hermetia illucens) (BS), mealworm meal (Tenebrio molitor) (MW), and a 1 : 1 mixture of both insect meals (BSMW) on fillet quality in African catfish (Clarias gariepinus). A total of 96 fish with an average initial body weight of 248 ± 28 g were stocked into a recirculating aquaculture system and fed in four different dietary groups (control, BS, MW, and BSMW). No mortality was recorded in any of the groups. At the end of the feeding period, 24 fish (n = 6 for each treatment, weight between 690 and 822 g) were used for analysis. There was no alteration in filleting yield or other slaughter indices within experimental groups, except the hepatosomatic index. Among quality attributes, pH 24 hr postmortem exhibited a significant difference (p < 0.05). In respect of the fatty acid profile, the n-6/n-3 ratio ranged between 1.17 and 1.40 but was not significantly modified by the partial replacement of FM. Similarly, the proximate composition of the fillets was not significantly different between the control and experimental diet groups. The ratio of polyunsaturated fatty acid to saturated fatty acids ranged between 0.67 and 0.79 in the fillets, without significant differences between groups. The atherogenic index was increased in the BS group, as compared to the others; however, the thrombogenicity index of fillets was not significantly affected. Similarly, the conventional quality traits of the fillet, such as cooking, drip, and thawing losses, did not differ within treatments. This study demonstrates that the dietary inclusion of black soldier fly and/or mealworm meals used for African catfish at the tested inclusion level has negligible impact on fillet properties.

Dried porcine soluble benefits to increase fish meal replacement with soy protein concentrate in large yellow croaker Larimichthys crocea diet

AbstractThe effect of dried porcine soluble (DPS) supplementation on replacement of dietary fish meal with soy protein concentrate (SPC) for large yellow croaker, Larimichthys crocea, was assessed in a feeding trial conducted in inshore net pens. Five isoproteic and isolipidic diets were tested. A basal diet (BD) contained 400 g/kg fish meal, and SPC was used as an alternative ingredient to replace 40% (F24) and 60% (F16) of the fish meal in BD at an equal protein basis. DPS was added at 5 g/kg in F24 (FD24) and F16 (FD16). Fish weighing 33.6 ± 7.4 g were fed with the test diets in 1.5 × 1.5 × 2 m net pens for 9 weeks. The fish meal replacement level significantly affected weight gain, feed conversion ratio, phosphorus retention efficiency, body phosphorus content, and ratio of fish meal consumption to fish production (RCP), while the DPS supplementation level significantly affected weight gain. The weight gain of fish fed FD24 was higher than that of fish fed F16, while no significant difference was found in weight gain between fish fed BD and fish fed FD24, F24, FD16 and F16. The feed cost, RCP and phosphorus waste of fish fed BD were higher than those of fish fed FD24, F24, FD16 and F16. Fish fed FD24 exhibited the best production performance, and the production performance of fish fed F24 and FD16 was close to that of fish fed BD. The present study indicates that the fish meal level in large yellow croaker diet could be lowered to 240 g/kg with SPC alone as a fish meal substitute, and could be further reduced to 160 g/kg by supplementation of 5 g/kg DPS. Therefore, DPS supplementation can benefit to increase fish meal replacement with SPC in large yellow croaker diet.

Effects of Dietary Fish Meal Replacement with Alternative Protein Ingredients and Their Combinations on Growth Performance, Feed Utilization, Fillet Composition, and Biochemical Parameters of Red Seabream (Pagrus major).

The experiment was conducted to evaluate alternative protein ingredients in a low-fish meal (FM) diet for red seabream (Pagrus major). Twelve experimental diets were formulated. Control diet (CON) was designed to contain 60% FM. Other experimental diets were formulated by replacing 50% of FM from the CON with soy protein concentrate (SPC), corn gluten (CG), meat meal (MM), and/or chicken byproduct meal (CBM). Four diets were designed including one of SPC, CG, MM, or CBM as FM replacer and designated as SPC, CG, MM, and CBM. Six other diets were formulated by adding two ingredients as SPC and CG, SPC and MM, SPC and CBM, CG and MM, CG and CBM, or MM and CBM, and designated as SCG, SMM, SCM, CMM, CCM, and MCM, respectively. The 12th diet (MIX) was formulated by including SPC, CGM, MM, and CBM. Triplicate fish groups (50.2 ± 0.1 g) were hand-fed for 12 weeks. Weight gain (WG) of fish was significantly improved by MM and MCM diets compared to CG, SCG, CMM, and CCM diets. WG of CON, SPC, CM, SMM, SCM, and MIX groups were comparable with MM and MCM groups. The lowest WG was observed in CG and CMM groups. Feed efficiency (FE) was significantly higher in MM group compared to SPC, CG, SGC, and CMC groups. FE of MCM group was significantly higher than CG and SCG groups. Fillet linolenic acid (C18:2n-6) level in CG group was significantly higher than CON, MM, CM, SCM, CCM, and MCM groups. Serum lysozyme activity was significantly higher in MCM and MIX groups. Therefore, a high level of dietary CG reduces the growth performance and feed utilization of red seabream. A mixture of MM and CBM seems to be more efficient in replacing FM from red seabream diet.

Replacement of dietary fish meal by fermented soybean meal improves growth, energy metabolism, and innate immune response in Penaeus vannamei Boone, 1931 (Decapoda: Dendrobranchiata: Penaeidae)

Abstract Fishmeal (FM) is considered a preferred source of protein for aquatic feed due to its good palatability and digestibility, a balanced amino acid profile, and a low level of anti-nutritional factors (ANFs). Shortage of FM supply and increases in price, however, are major challenges faced by feed producers and aquaculture farmers. Fermented soybean meal (FSBM) has been proposed to partially replace FM because of its high nutritional value and low cost. The optimal FSBM concentration for substituting FM and effects of FSBM substitution on growth performance, energy metabolism, and immune response nevertheless remain unclear. We evaluated the effects of shrimp diets containing FSBM at 0% (FM), 25% (FSBM25), 50% (FSBM50), 75% (FSBM75), and 100% (FSBM100) on the shrimp Penaeus vannameiBoone, 1931. FSBM50 led to a significantly higher weight gain (postlarvae 1927.36 ± 46.64%, young juveniles 237.00 ± 8.25%, and juveniles 153.96 ± 2.18%) than the other experimental and control diets in different stages of the shrimp. The FM group consumed less oxygen than the experimental diet groups. FSBM75 and FSBM100 led to a significantly higher ammonia-N excretion than FM, FSBM25, and FBSM50. All experimental diet groups exhibited a significantly higher O:N ratio (8.3 ± 0.8–9.4 ± 1.7) than the FM group (7.1 ± 0.2). Shrimp fed with FSBM25- and FSBM50-containing diets exhibited an increased total hemocyte count, and FSBM25, FBSM50, and FSBM100 led to a significantly higher superoxide anion production than FSBM75 and FM. FSBM substitution in shrimp diets resulted in a significantly higher phagocytic activity than the FM diet, suggesting that FSBM substitution improves the cell-mediated immune response in shrimps. In challenge trials with white spot syndrome virus (WSSV), all experimental diets led to a higher survival rate than the FM diet, and in Vibrio parahaemolyticus challenge trials, FSBM50 led to a higher survival rate than the other experimental and control diets, indicating that FSBM substitution in shrimp diets could improve the defense capacity of shrimps against WSSV and V. parahaemolyticus.

Effects of dietary replacement of fishmeal by cottonseed meal on the growth performance, immune and antioxidant responses, and muscle quality of juvenile crayfish Procambarus clarkii

The current research was aimed to determine the impacts of cottonseed meal substitution levels for fishmeal on the growth performance, immune indices, antioxidant ability, and muscle texture of red swamp crayfish (Procambarus clarkii). Five isonitrogenous and isolipidic diets were formulated to feed the crayfish for 8 weeks. The basal diet contained 20% fishmeal (FM), while the other four experimental diets substituted fishmeal in FM diet with cottonseed meal at levels of 25%, 50%, 75%, and 100%, referred to as CM25, CM50, CM75, and CM100 respectively. A total of 750 crayfish with an average initial weight of 4.00 g were selected and randomly allocated to fifteen cages. Each diet was randomly assigned to triplicate groups of crayfish. Weight gain and specific growth rate declined, and feed conversion rate, asparate aminotransferase and alanine transaminase activities appear to increase as cottonseed meal substitution levels increased. Serum total cholesterol levels declined in crayfish fed CM100 diet compared to crayfish fed the FM diet. Serum phenoloxidase and lysozyme activities, and muscle hardness and mef-2a mRNA expression were the highest for crayfish fed fishmeal-based diet and showed no significant difference among other four groups. Dietary cottonseed meal levels did not significantly influence hepatopancreas index, viscerosomatic index, condition factor, serum glucose, triglyceride, albumin, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol and creatinine contents, as well as the contents of acid phosphatase, and alkaline phosphatase, and muscle texture profile (cohesiveness, springiness, adhesiveness, and chewiness) and mef-2b and mstn mRNA expression. High dietary fish meal replacement by cottonseed meal tended to upregulate mRNA expression of nuclear factor erythroid 2-related factor 2, glutathione peroxidase, glutathione reductase, manganese superoxide dismutase, glutathione S-transferase, and catalase. Taken together, dietary cottonseed meal reduced growth performance and feed utilization, induced hepatopancreas oxidative stress, and influenced some serum and muscle parameters. The gossypol content and unbalanced amino acid profile are considered the limiting factor influencing the fish meal replacement by cottonseed meal.

Replacement of Dietary Fishmeal with Clostridium autoethanogenum Protein on Lipidomics and Lipid Metabolism in Muscle of Pearl Gentian Grouper.

Clostridium autoethanogenum protein (CAP) is an economical and alternative protein source. Here, three experimental diets were formulated with CAP replacing 0% (CAP-0), 30% (CAP-30), and 60% (CAP-60) of fishmeal to investigate the alterations of structure integrity, fatty acids profiles, and lipid metabolism in the muscle of pearl gentian grouper. With increasing levels of CAP substitution, the percentages of 16 : 0 or 18 : 0 were decreased in triglycerides (TG) and diacylglycerols (DG); 18 : 1 or 18 : 2 was increased at the sn-1 and sn-2 positions in phosphatidylethanolamines; 20 : 5n-3 was increased in TG and DG. The phosphatidylcholines (PC) (18 : 3/20 : 5), PC(22 : 6/17 : 1), and sphingomyelins (d19 : 0/24 : 4) were identified as potential lipid biomarkers between CAP treatments. The CAP-30 treatment enhanced lipolysis and lipogenesis, while the CAP-60 treatment inhibited lipogenesis. In conclusion, fishmeal replacement with CAP affected the lipid characteristics and lipid metabolism, whereas it did not affect the structural integrity and fatty acids profiles in the muscle of pearl gentian grouper.

Substituting Fish Meal with Tubiechong (Eupolyphaga sinensis) By-Product in the Diets of Largemouth Bass (Micropterus salmoides): Effects on Growth, Meat Quality, and Liver Health.

A feeding trial was conducted to evaluate the effect of replacing 0% (control), 10% (T10), 20% (T20), 30% (T30), and 40% (T40) fish meal with a Tubiechong (Eupolyphaga sinensis) by-product in largemouth bass (Micropterus salmoides). Triplicate groups of 30 fish (5.36 ± 0.01 g) were fed two times daily to apparent satiation for 60 days. The experimental results showed that the Tubiechong by-product could improve the growth performance of largemouth bass by increasing the FBW, WGR, and SGR until the replacement ratio was 40%. The quadratic regression analysis showed that the proportion of the Tubiechong by-product was 20.79% and 20.91%, respectively, when WGR and SGR were the best. Concurrently, the meat quality in the replacement groups was higher, specifically, the lightness and white values were higher, and the water loss rates were lower (P < 0.05) than that in the control group. Moreover, the changes of the activities of CAT and GSH in the liver and T-AOC and GSH in serum could reveal the antioxidant capacity improvement of fish by the Tubiechong by-product. In the study, the replacement groups had lower T-CHO and HDL-C in serum (P < 0.05), indicating that the Tubiechong by-product had an active role in improving blood lipid and regulating lipid metabolism. Simultaneously, the replacement groups had a normal structure with central hepatocytes' nuclei and deviated from the center partly, while most of the hepatocytes were swollen in the control group with nuclear degeneration. The results showed that the Tubiechong by-product had a positive effect on the liver health of fish. Conclusively, the present study indicated that the partial dietary replacement of fish meal using the Tubiechong by-product (for up to 40% replacement level) in the diet of largemouth bass not only caused no adverse effects on fish health but also improved the growth performance, meat quality, antioxidant capacity, and hepatic health and is conducive to supplying nutritious, high-quality, and healthy aquatic products.

Dietary Fishmeal Can Be Partially Replaced with Non-Grain Compound Proteins through Evaluating the Growth, Biochemical Indexes, and Muscle Quality in Marine Teleost Trachinotus ovatus.

In the context of human food shortages, the incorporation of non-grain feedstuff in fish feed deserves more research attention. Here, the feasibility and appropriate ratio of non-grain compound protein (NGCP, containing bovine bone meal, dephenolized cottonseed protein, and blood cell meal) for dietary fishmeal (FM) replacement were explored in golden pompano (Trachinotus ovatus). Four isonitrogenous (45%) and isolipidic (12%) diets (Control, 25NGP, 50NGP, and 75NGP) were prepared. Control contained 24% FM, whereas the FM content of 25NGP, 50NGP, and 75NGP was 18%, 12%, and 6%, respectively, representing a 25%, 50%, and 75% replacement of FM in Control by NGCP. Juvenile golden pompano (initial weight: 9.71 ± 0.04 g) were fed the four diets for 65 days in sea cages. There was no significant difference between the 25NGP and Control groups in terms of weight gain, weight gain rate, and specific growth rate; contents of crude protein, crude lipid, moisture, and ash in muscle and whole fish; muscle textural properties including hardness, chewiness, gumminess, tenderness, springiness, and cohesiveness; and serum biochemical indexes including total protein, albumin, blood urea nitrogen, HDL cholesterol, total cholesterol, and triglycerides. However, the golden pompano in 50NGP and 75NGP experienced nutritional stress, and thus some indicators were negatively affected. In addition, compared to the Control group, the expression levels of genes related to protein metabolism (mtor, s6k1, and 4e-bp1) and lipid metabolism (pparγ, fas, srebp1, and acc1) of the 25NGP group showed no significant difference, but the 4e-bp1 and pparγ of the 75NGP group were significantly upregulated and downregulated, respectively (p < 0.05), which may explain the decline in fish growth performance and muscle quality after 75% FM was replaced by NGCP. The results suggest that at least 25% FM of Control can be replaced by NGCP, achieving a dietary FM content of as low as 18%; however, the replacement of more than 50% of the dietary FM negatively affects the growth and muscle quality of golden pompano.

Impact of temperature and dietary replacement of fishmeal on cardiovascular remodelling and growth performance of adult Atlantic salmon (Salmo salar L.)

The development of pathophysiological cardiac alterations in farmed salmonids is a recurrent and widespread welfare issue. Although the aetiology of such cardiac alterations is still unclear, several factors, including rearing temperature, selective breeding programs, and feeding strategies, could induce a morpho-physiological remodelling of cardiac structure. This maladaptive cardiac remodelling could impair cardiac function and health of farmed salmonids leading to premature mortality and production losses. In this study, we aimed to better understand the impact of suboptimal elevated temperature, resembling a typical summer temperature in Tasmania, and different feed formulations with low inclusion of fishmeal on cardiovascular remodelling and growth performance of adult Tasmanian Atlantic salmon. Fish were reared in a seawater recirculating aquaculture system and fed one of three diets with different fishmeal contents (6, 12, and 18%), while exposed to a temperature profile characterised by an incremental increase (1 °C per day) from 15 to 19.5 °C, 84 days at 19.5 °C (elevated temperature), and finally 34 days at 15 °C (optimal temperature). In terms of cardiac morphometric parameters, ventricular roundness (measured as height:width ratio) was not affected by temperature or dietary treatment. Conversely, the angle between the bulbus arteriosus and the ventricle significantly increased after the elevated temperature phase, along with relative ventricular mass, showing signs of maladaptive remodelling compared to initial conditions. However, these cardiac parameters were not different between the elevated and optimal temperature phase, which was associated with no changes in transcript abundance of molecular markers of pathological hypertrophy and remodelling, such as, atrial natriuretic peptide (anp) and b-type natriuretic peptide (bnp). Collectively, standard growth rate (SGR) and temperature growth coefficient (TGC), along with feed conversion ratio (FCR), were significantly elevated following exposure to suboptimum elevated temperature relative to optimal temperature. However, no differences in growth rate were observed in fish fed distinct dietary treatments at both elevated and optimal temperatures. These results imply that feeds with low levels of fishmeal can be used under challenging Tasmanian summer conditions without detrimental effect on salmon growth performance. Furthermore, our findings suggest that suboptimal elevated temperatures may induce remodelling of cardiac structure in adult Atlantic salmon, thereby potentially compromising cardiac function and overall health. Future efforts to manage Atlantic salmon susceptibility to climate change will require further research on the effect of challenging environmental conditions on cardiac physiology and performance in an attempt to prevent cardiac pathology, thereby improving health and welfare of farmed salmonids.

Effect of replacement of dietary fish meal with silkworm pupae meal and black soldier fly larvae meal as a combination diet on the growth and digestive performance of koi carp Cyprinus carpio var. koi in the nursery phase

The feasibility of using silkworm pupae meal and black soldier fly larvae meal in combination to replace fish meal inkoi carp fry was assessed. Two weeks-old koi fry (2.47±0.15 cm, 0.28±0.06 g) were used as experimental animals. Fouriso-nitrogenous experimental diets (T1, T2, T3, T4) with 35±0.37% crude protein were formulated. In T1, T2 and T3,50% of fish meal was replaced with silkworm pupae meal and black soldier fly larvae meal at different combinations, i.e.,T1 (25:75), T2 (50:50), T3 (75:25). In T4, 100% of fish meal was replaced with silkworm pupae meal and black soldier flylarvae meal at 50:50 ratio. Present research findings showed that T2 diet-fed fishes performed significantly better (p&lt;0.05)than other diets in all the growth parameters viz., mean length gain (3.13±0.01 cm), mean weight gain (3.13±0.02 g),percentage length gain (127±0.71%), percentage weight gain (1118±7.07%), specific growth rate (4.16±0.01%/day),feed conversion ratio (1.30±0.01), feed efficiency ratio (0.76±0.01), protein efficiency ratio (2.18±0.01) and survival rate(28±1.41%). Digestive enzyme activities were also higher in T2 diet-fed fishes. Therefore, insect meal can be effectivelyutilised to replace fish meal in the diet of koi fry. Keywords: Black soldier fly larvae meal, Digestive enzymes, Growth performance, Koi carp fry, Silkworm pupae meal

Effects of poultry by-product meal replacing fish meal on growth performance, feed utilization, intestinal morphology and microbiota communities in juvenile large yellow croaker (Larimichthys crocea)

An 8 weeks feeding trail was conducted to evaluate the potential of poultry by-product meal (PBM) replacing fish meal (FM) in the diet of juvenile large yellow croaker (Larimichthys crocea). Five iso-nitrogenous (45% crude protein), iso-lipidic (10% crude lipid) and iso-energetic (about kcal 400 g diet −1) diets were formulated with 0, 25%, 50% 75% and 100% FM being replaced by PBM (marked as FM, PBM25, PBM50, PBM75 and PBM100). Crystal methionine and lysine were added into PBM-containing diets to match its concentration in FM diet. Each diet was randomly assigned to triplicate groups of 30 fish (initial weight 18.43 ± 1.28 g) in floating sea cages. The weight gain, specific growth rate and feed efficiency of L. crocea decreased significantly when the replacement level of FM was higher than 25%. The feed intake of fish fed PBM100 was significantly lower than those fed FM, PBM25 and PBM50. Juveniles fed PBM-containing diets showed significantly lower value of muscular thickness in intestine when compared to those fed FM. Villi length decreased significantly when the replacement of FM in diets was more than 50%. Fish fed FM diet had the relative higher abundances of Exiguobacterium (from order to genus), Bacillus (from order to genus), Pseudomonadales (from order to genus) and Acinetobacter genus, those fed PBM25, PBM50 and/or PBM75 showed higher abundance of Peptostreptococcaceae and Vibrio. Overall, PBM replacing dietary FM may inhibit growth performance of L. crocea through reducing digestibility via declining the abundance of intestinal bacteria that producing digestive enzyme, and improving the abundance of pathogen. Peptostreptococcaceae and Vibrio could be the indicator in the intestinal of L. crocea fed high PBM diets. The optimum dietary FM replacement level by PBM was 25% for L. crocea.

Partial dietary fishmeal replacement with mixture of plant protein sources supplemented with exogenous enzymes modify growth performance, digestibility, intestinal morphology, haemato-biochemical and immune responses for Nile tilapia, Oreochromis niloticus

For the aquaculture sector, expanding the amount of plant proteins substituting fish meal (FM) is favorable. This study was conducted to evaluate the properties of diets containing a blend of plant protein sources (cotton seed meal, sunflower meal and jojoba (CSJ)) instead of FM and supplemented with a mixture of exogenous enzymes on the growth performance, intestinal morphology, blood indices, activity of endogenous and digestibility also, immune response of Nile tilapia, Oreochromis niloticus. Three groups of the experimental diet (29.5% crude protein) and (18.76 MJ kg −1 gross energy) were performed. The first group CSJ1 was (FM: CSJ = 2:1), the second group diet CSJ2 was (FM: CSM = 2:2) and the third CSJ3 was (FM: CSJ = 2:1), the replaced was based on content of protein. All groups of diets were supplemented with mixture of enzymes (Protease, pepsin, trypsin, xylanase and amylase) at 0 and 0.5 g kg −1 diet therefore six diet was formulated. Fingerlings (6.30 ± 0.09 g) were randomly allocated into eighteen plastic tank (450 L capacity) in triplicate per each treatment (25 fish/ treatment) for 90 days. After 90-days of the feeding trial, results indicated that the highest wight gain and best FCR, higher (P < 0.05) activity of trypsin and lipase, apparent digestibility coefficient of nutrients were detected in fish fed diet of FM: CSJ 2:1 and 2:2 supplemented with 0.5 g enzymes mixture kg−1 diet with insignificant differences between group. The diet incorporating FM: CSJ 2:1 with 0.5 g enzymes mixture showed the maximum (P < 0.05) villi length/width, ASA, and MSR values. Fish fed a diet containing FM: CSJ 1:2, and supplemented with 0.5 g of an enzyme mixture kg−1 diet had the maximum Hb, Htc, RBCs and WBCs values (P < 0.05). The highest (P < 0.05) TP and GL value was estimated in diet containing FM: CSJ; 1:2 without the addition of enzymes mixture. while, the best (P < 0.05) value of ALT and AST were showed in diet containing FM: CSJ 2:1 without the addition of enzymes. The highest TAC, GSH and SOD values were recorded by fish fed diet containing FM: CSJ 1:2 or 2:2 with 0.5 g enzymes mixture kg−1 diet. Fish fed diets comprising various ratios of FM: CSJ with 0.5 g enzymes mixture kg−1 diet had the lowest (P < 0.05) MDA value. In conclusion, using mixture of enzymes (protease, pepsin, trypsin, xylanase and amylase) at 0.5 g kg−1 in combination with a high blend of CSJ instead of FM; 2:2 improved the growth performance, digestibility, digestive enzyme, intestinal morphology, blood indices, and immune response of Nile tilapia.

Growth Performance, Antioxidant and Immunity Capacity Were Significantly Affected by Feeding Fermented Soybean Meal in Juvenile Coho Salmon (Oncorhynchus kisutch)

This study aims to investigate the effects of partial dietary replacement of fish meal with unfermented and/or fermented soybean meal (fermented by Bacillus cereus) supplemented on the growth performance, whole-body composition, antioxidant and immunity capacity, and their related gene expression of juvenile coho salmon (Oncorhynchus kisutch). Four groups of juveniles (initial weight 159.63 ± 9.54 g) at 6 months of age in triplicate were fed for 12 weeks on four different iso-nitrogen (about 41% dietary protein) and iso-lipid (about 15% dietary lipid) experimental diets. The main results were: Compared with the control diet, the diet with replaced 10% fish meal protein with fermented soybean meal protein supplementation can significantly (p < 0.05) influence the expression of superoxide dismutase, catalase, glutathione peroxidase, glutathione S-transferase, nuclear factor erythroid 2-related factor 2, tumor necrosis factor α and interleukin-6 genes, the growth performance, the serum biochemical indices, and the activity of antioxidant and immunity enzymes. However, there was no significant effect (p > 0.05) on the survival rate (SR) and whole-body composition in the juveniles among the experimental groups. In conclusion, the diet with replaced 10% fish meal protein with fermented soybean meal protein supplementation could significantly increase the growth performance, antioxidant and immunity capacity, and their related gene expression of juveniles.

Effects of the Replacement of Dietary Fish Meal with Poultry By-Product Meal on Growth and Intestinal Health of Chinese Soft-Shelled Turtle (Pelodiscus sinensis).

To investigate the effect of poultry by-product meal (PBM) replacing fish meal on the growth and intestinal health of Chinese soft-shelled turtle (Pelodiscus sinensis). Four experimental diets were prepared. Fish meal was replaced by 0 (control group, PBM0), 5% (PBM5), 10% (PBM10), and 15% (PBM15) PBM. Compared to the control group, final body weight, weight gain, and specific growth rate were significantly increased, while feed conversion rate decreased significantly in the PBM10 group (p < 0.05). The PBM15 group significantly increased the moisture content and significantly decreased the ash content of the turtles (p < 0.05). The PBM5 and PBM15 groups significantly decreased the whole-body crude lipid (p < 0.05). The serum glucose content increased significantly in the PBM10 group (p < 0.05). The liver malonaldehyde content significantly decreased in the PBM5 group and in the PBM10 group (p < 0.05). Liver glutamic-oxalacetic transaminase and intestinal pepsin activity were increased significantly in the PBM15 group (p < 0.05). The expression of the intestinal interleukin 10 (IL-10) gene was significantly down-regulated in the PBM10 group and the PBM15 group (p < 0.05), the expression of the intestinal interferon-γ (IFN-γ), interleukin-8 (IL-8), and liver toll-like receptor 4 (TLR4) and toll-like receptor 5 (TLR5) genes were significantly up-regulated in the PBM5 group (p < 0.05). In summary, poultry by-product meal can be used as a protein source to replace fish meal in turtle feed. Based on quadratic regression analysis, the optimal replacement ratio is 7.39%.

Effects of dietary replacement of fish meal by soybean meal on growth, feed utilization, and health condition of stinging catfish, Heteropneustes fossilis

Soybean meal (SBM) is a cost-effective alternative protein source to replace costly fish meal in aquaculture. This present study determined to measure the effects of replacing fish meal (FM) protein with SBM on growth, feed utilization, and health condition of stinging catfish,Heteropneustes fossilis. Four isonitrogenous (35 %) diets were applied in four treatment groups designed as SBM0, SBM25, SBM50, and SBM75, where 0 %, 25 %, 50 %, and 75 % of FM protein were substituted by SBM, respectively. Significantly higher mean final weight (g), weight gain (g), percent weight gain (%), specific growth rate (% day−1), and protein efficiency ratio (PER) were recorded in SBM0, SBM25, and SBM50 groups than SBM75 group. Consequently, significantly lower feed conversion ratio (FCR) was found in SBM0, SBM25, and SBM50 groups than SBM75 group. Moreover, protein content of whole-body carcass was significantly higher in SBM25 and lower in SBM0 group however, lipid content was significantly higher in SBM0 and SBM75 than in other groups. Hemoglobin, red blood cells, and white blood cells were significantly higher in SBM0, SBM25, and SBM50 groups compared to SBM75. However, the higher the substitution of FM protein by SBM in diets higher the values of glucose. Morphological analysis of the intestine including villi length (μm), width (μm), and area (mm2); crypt depth (μm); wall thickness (μm); abundance of goblet cell (GB); and muscle thickness (μm) showed an increasing trend in fish fed diet containing upto 50 % replacement of FM protein by SBM. Therefore, the results suggest that SBM could replace upto 50 % FM protein in diets ofH. fossiliswithout compromising growth, feed efficiency, and health status.

Dietary fishmeal replacement with Hermetia illucens (Black soldier fly, BSF) larvae meal affected production performance, whole body composition, antioxidant status, and health of snakehead (Channa striata) juveniles

Due to the market instability and increasing demand and prices of fishmeal, the aquafeed industry must seek alternative protein feedstuffs to keep producing aquafeeds in a sustainable and cost-effective way. Hermetia illucens (BSF) larvae meal is considered sustainable since its production recycles organic waste streams into a valuable nutrient. Therefore, this study assessed the effects of dietary fish meal (FM) replacement with Hermetia illucens larvae meal (HIM) on the production performance of snakehead (Channa striata) juveniles. Five isoproteic (420 g Kg−1) and isocaloric (19.5 MJ kg−1 diet) experimental diets were designed by replacing FM at 0% (control diet), 25% (HIM25), 50% (HIM50), 75% (HIM75), and 100% (HIM100) with HIM. Four hundred and fifty juveniles (16.36 ± 0.09 g fish−1) were randomly assigned to 15 tanks (400 litres; 30 fish tank−1) and fed respective diets twice daily until apparent satiety for 9 weeks. The weight gain (WG), specific growth rate (SGR), and protein efficiency ratio (PER) showed a linear decrease with increasing dietary HIM (P < 0.05), and no significant effect in fish fed control, HIM25, and HIM50 diets. Whole body proximate composition and organ somatic indices were unaffected (P > 0.05). Whole-body C12, C14, saturated fatty acid (SFA), and SFA: PUFA ratios increased linearly with increasing FM replacement whereas polyunsaturated fatty acid (PUFA) and n3/n6 fatty acid ratios decreased linearly (P < 0.05). Dietary HIM had a significant effect on protease activity (P < 0.05), but had no effect on lipase and amylase activity (P > 0.05). With increasing levels of dietary HIM, the apparent digestibility coefficient (ADC) of dry matter linearly decreased, and the ADC of crude protein showed significant linear and quadratic decrease, but the ADC of crude lipid was unaffected (P > 0.05). Aspartate aminotransferase (ASAT) and alanine aminotransferase (ALAT) were unaffected by HIM levels (P > 0.05). Fish fed HIM incorporated diets had higher superoxide dismutase (SOD) and catalase (CAT) activities than fish fed a control diet, with a linear and quadratic effect (P < 0.05). The liver glutathione peroxidase (GPx) was unaffected (P > 0.05), whereas plasma GPx increased linearly and quadratically as dietary HIM levels increased (P < 0.05). Malonaldehyde (MDA) levels in liver and plasma remained unchanged among all the groups (P > 0.05). The dietary HIM did not affect the blood biochemistry (NBT, glucose, Hb, RBC, and WBC) and plasma metabolites (total protein, albumin, globulin, and total lipids) (P > 0.05). The lower plasma cholesterol was witnessed in fish fed HIM-incorporated diets than in fish fed control diets except for the HIM50 diet (P < 0.05). In conclusion, HIM can replace dietary FM up to 50% (20 g kg−1 HIM inclusion) without harming the production performance and health of snakehead juveniles.

Effects of Dietary Replacement of Fish Meal by Raw and Fermented Soybean Residues on Growth Performance, Biological Parameters and Nutrient Digestibility in Red Tilapia Fish (Oreochromis sp.)

Effects of Dietary Replacement of Fish Meal by Raw and Fermented Soybean Residues on Growth Performance, Biological Parameters and Nutrient Digestibility in Red Tilapia Fish (Oreochromis sp.)

Replacement of Dietary Fishmeal by Black Soldier Fly Larvae (Hermetia illucens) Meal in Practical Diets for Juvenile Tench (Tinca tinca)

The development of specific diets for the juvenile stage is a main target for culture intensification of tench (Tinca tinca). Aquafeeds still rely heavily on the use of fishmeal (FM) but concerns about economic and ecological sustainability make the use of alternative protein sources necessary. Insect meals are considered a promising substitute to replace FM. In a 90-day experiment, 6 diets with different replacement levels of FM by partially defatted black soldier fly larvae meal (BSFLM): 0%, 15%, 30%, 45%, 60% and 75%, were tested on juvenile tench. Survival rates were high (95.8–100%) without differences between treatments. Diet with 45% FM replacement allowed for best growth performance in terms of total length (TL = 66.4 mm) and weight (W = 4.19 g), without differences with 60% and 75% of replacement. A cubic relationship was evidenced between the level of FM replacement and growth. From the regression equations, the estimated optimal level of FM replacement was 47% (356 g BSFLM kg−1 diet). Externally visible deformities were always under 0.05%. The whole-body lipid content of the fish had a significantly negative linear regression with BSFLM (r2 = 0.80). The content of the essential amino acids (EAA) arginine, leucine, lysine, phenylalanine, methionine, and threonine in diets decreased with dietary BSFLM inclusion. However, it did not have a negative effect on growth performance, suggesting that EAA requirements were covered. The amount of essential amino acids in whole-body juveniles was similar independently of the diet provided. The results allow considering BSFLM as a sustainable protein source for juvenile tench feeding.

A study of the potential effect of dietary fishmeal replacement with cricket meal (Gryllus bimaculatus) on growth performance, blood health, liver antioxidant activities, intestinal microbiota and immune-related gene expression of juvenile channel catfish

Studies have shown that insect meals can partially or completely replace fish meal (FM) that are commonly used in aquaculture. Therefore, a 10-week feeding trail was done to test the potential impacts of replacement of FM with cricket meal (CM) on the growth performance, body composition, blood health, liver antioxidant activities, intestinal microbiota and immune reaction of juvenile channel catfish. Five isonitrogenous (crude protein 35%) and isocaloric (19 MJ/kg−1) diets were formulated to have 0%, 25%, 50%,75% and 100% CM and named T0, T25, T50, T75 and T100 groups, respectively. Final body weight (FBW), weight gain rate (WGR) and specific growth rate (SGR) in fish fed T50 and T75 groups were considerably elevated compared with fish fed control T0 group. Higher hepatosomatic index (HSI) was found in fish fed T50, T75 and T100 groups compared with fish fed control T0 group. The moisture and crude protein contents in fish fed T75 and T100 groups were significantly increased than that of fish fed control T0 group. Hematological parameters examination of juvenile channel catfish demonstrated that hemoglobin (HGB), hematocrit (HCT), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) showed significantly higher values with increasing content of CM in dietary fish fed groups. Malondialdehyde (MDA) content and glutathione peroxidase (GPx) activity level were highest in fish fed T75 group, respectively. Catalase (CAT) activity in fish fed T50 to T100 groups was significantly greater than those of fish fed control T0 and fish fed T25 groups. Intestinal microbiota sequencing analysis showed that Chao1, Shannon, Simpson indices, and Good’s coverage in fish fed control T0, fish fed T50 and T100 groups were not significantly affected. Intestinal microbiota analysis showed the relative abundance of Proteobacteria and Firmicutes were dominated at the phyla level. Moreover, with an increasing of CM replacement, the abundance of a prominent pathogenic bacterial Acinetobacter was reduced at the genus level although no significant differences. The immune-related gene expression levels of IL-1β, IL-8, IL-10 and HIF1α were up-regulated in intestine when CM replacement increased from 0% to 75%, and higher levels were observed in fish fed T75 group comparing to others. The expression levels of TNF-α, IL-22 and IFN-γ were significantly elevated in fish fed T100 group. Notably, fish fed T50 to T100 groups down-regulated the expression of NF-κB compared to the other groups. To conclude, the present findings show that CM could enhance growth performance, improving blood health and liver antioxidant capacity, and substantial alterations in intestinal microbial composition of juvenile channel catfish, suggesting that FM could be replaced by CM as insect protein source in channel catfish diet.