Fish Industry Research Articles

A challenge test on Pseudomonas spp. as spoiling microorganism in fish fillets

Fish fillets are highly susceptible to spoilage, with Pseudomonas spp. bacteria being among the main culprits. To maintain products’ quality and safety, it is important to control the load of these microorganisms and understand their growth potential in fish fillets. However, setting up challenge tests might be hard due to the difficulty of differentiating intentionally inoculated bacteria from those already present on the fillets. To overcome this obstacle, a pilot study using Pseudomonas aeruginosa, a clinically significant bacterial species that is rare in food, was conducted. Vacuum-packed Northern cod, salmon, and plaice fish fillets were experimentally inoculated and subjected to trials at both refrigeration (4 °C) and thermal abuse temperatures (from +4 °C to +6 °C and then to +8 °C). The results showed that the growth potential of Pseudomonas aeruginosa in all the fish fillets was less than 0.5 Log10 CFU/g. This confirms that vacuum packaging could reduce the multiplication of Pseudomonas spp. in the fish fillets and underlines as it is crucial to have fish fillets containing initial loads of Pseudomonas between 104-105 CFU/g or lower at the beginning of the shelf life in order to control the deterioration rate of the product. This study provides a basis for developing further challenge tests for Pseudomonas spp. in the fish industry and highlights the importance of controlling initial loads of Pseudomonas to prevent product deterioration during the shelf life.

Effects of Different Farming Modes on Salmo trutta fario Growth and Intestinal Microbial Community.

The gut microbiota plays a pivotal role in upholding intestinal health, fostering intestinal development, fortifying organisms against pathogen intrusion, regulating nutrient absorption, and managing the body's lipid metabolism. However, the influence of different cultivation modes on the growth indices and intestinal microbes of Salmo trutta fario remains underexplored. In this study, we employed high-throughput sequencing and bioinformatics techniques to scrutinize the intestinal microbiota in three farming modes: traditional pond aquaculture (TPA), recirculating aquaculture (RA), and flow-through aquaculture (FTA). We aimed to assess the impact of different farming methods on the water environment and Salmo trutta fario's growth performance. Our findings revealed that the final weight and weight gain rate in the FTA model surpassed those in the other two. Substantial disparities were observed in the composition, relative abundance, and diversity of Salmo trutta fario gut microbiota under different aquaculture modes. Notably, the dominant genera of Salmo trutta fario gut microbiota varied across farming modes: for instance, in the FTA model, the most prevalent genera were SC-I-84 (7.34%), Subgroup_6 (9.93%), and UTCFX1 (6.71%), while, under RA farming, they were Bacteroidetes_vadinHA17 (10.61%), MBNT15 (7.09%), and Anaeromyxoactor (6.62%). In the TPA model, dominant genera in the gut microbiota included Anaeromyxobacter (8.72%), Bacteroidetes_vadinHA17 (8.30%), and Geobacter (12.54%). From a comparative standpoint, the genus-level composition of the gut microbiota in the RA and TPA models exhibited relative similarity. The gut microbiota in the FTA model showcased the most intricate functional diversity, while TPA farming displayed a more intricate interaction pattern with the gut microbiota. Transparency, pH, dissolved oxygen, conductivity, total dissolved solids, and temperature emerged as pivotal factors influencing Salmo trutta fario gut microbiota under diverse farming conditions. These research findings offer valuable scientific insights for fostering healthy aquaculture practices and disease prevention and control measures for Salmo trutta fario, holding substantial significance for the sustainable development of the cold-water fish industry in the Qinghai-Tibet Plateau.

Status and prospects of the ornamental fish industry in Kenya

AbstractKenya is a major player in the global ornamental trade, exporting substantial quantities of ornamental fish to the global market. The sector plays an important role in the country's economy through foreign exchange. However, the country's ornamental fish industry has not fully developed to generate more economic benefits. This paper sought to identify the existing opportunities for enhancing the production and trade of ornamental fish in the country. The findings reveal that there are opportunities for collection of native freshwater ornamental fish species from the vast freshwater resources in the country; for the culture of marine ornamental fish; for public aquariums as centres of recreation, conservation, research and education; and for employment and development of support industries. The findings also show that there are international legislations and national policies that create an enabling environment for the growth of the industry. To unlock the full potential of the industry, training and research, investment and infrastructure development, quality assurance, collaboration and networking, and environmental sustainability strategies need to be put in place. This will ensure Kenya's position as a key player in the global ornamental fish trade.

Chironomid farming in consideration of various physicochemical parameters and reuse of condensate water

AbstractLarvae of chironomids are benthic organisms, having the extreme capability to adapt to the changing physicochemical parameters of the aquatic body and the potentiality to accommodate that adverse situation. In spite of the demand for chironomid larvae as fish food, no proper technique of chironomid farming has been reported till today. This instance encourages the research on farming these larvae, in both open and captive settings. The present research shows a good result all over the year, with seasonal variance along with changing physicochemical parameters. This farming is based on minimal space requirements, reuse of condensate water, and the application of a low‐cost diet, resulting in enormous production of this insect. Thus, it can be placed as a small‐scale industry to uplift the economic status of rural people. A nutritious diet for both carnivorous and omnivorous fish and low‐cost farming with a huge production of these larvae is good for the fish industry as well.

The Effect of Sumac (Rhus coriaria L.) Application on Oxidation Status, Sensory Attributes, Physicochemical and Microbiological Parameters of Carp (Cyprinus carpio) Fish during Chilled Storage

The aim of this study was to evaluate the impact of sumac on the oxidation status, sensory properties, and physicochemical and microbiological parameters of common carp (Cyprinus carpio) fish during chilled storage. Fillets of carp were treated with ground sumac at percentages of 0% (T0), 0.5% (T1), 1.5% (T2) and 2.5% (T3) and analysed after 24 and 72 h of chilled storage. The fat content, oxidation parameters (thiobarbituric acid reactive substances, TBARS), antioxidant capacity, total volatile basic nitrogen (TVB-N), free fatty acids (FFA), sensory properties, colour indicators (lightness L*, redness a*, yellowness b*), water activity, pH value, aerobic plate count (APC), psychrotrophic microorganism count (PMC), and coliform bacteria count were determined. The TBARS values of T1, T2, and T3 decreased significantly (p < 0.05) after 24 h of chilled storage. The antioxidant capacity increased in a dose-dependent and time-dependent manner. As regards organoleptic properties, sumac was able to reduce the natural fishy odour, increase the sour odour and flavour, and mask the fish odour and flavour significantly. A significant effect of sumac on the redness (a*) values of muscles was observed, which increased with elevation of the sumac ratio. A decrease in fillet pH and lower APC and PMC were observed. No effect was found on water activity or the growth of coliform bacteria. The study found that sumac plays a role in the oxidation status, pH value and organoleptic properties of fish fillets, which could be beneficial for the fish and fishery products industry.

Deploying a Computer Vision Model Based on YOLOv8 Suitable for Drones in the Tuna Fishing and Aquaculture Industry

In recent years, the global tuna fishing and aquaculture industry has encountered significant challenges in balancing operational efficiency with sustainable resource management. This study introduces an innovative approach utilizing an advanced computer vision model, PA-YOLOv8, specifically adapted for drones, to enhance the monitoring and management of tuna populations. PA-YOLOv8 leverages the capabilities of YOLOv8, a state-of-the-art object detection system known for its precision and speed, tailored to address the unique demands of aerial surveillance in marine environments. Through comprehensive modifications including downsampling techniques, feature fusion enhancements, and the integration of the Global Attention Module (GAM), the model significantly improves the detection accuracy of small and juvenile tuna within complex aquatic landscapes. Experimental results using the Tuna dataset from Roboflow demonstrate marked improvements in detection metrics such as precision, recall, and mean average precision (mAP), affirming the model’s effectiveness. This study underscores the potential of integrating cutting-edge technologies like UAVs and computer vision in promoting sustainable practices in the aquaculture sector, setting a new standard for technological applications in environmental and resource management. The advancements presented here provide a scalable and efficient solution for real-time monitoring, contributing to the long-term sustainability of marine ecosystems.

Virulent and Multi-drug-Resistant Edwardsiella tarda Infection in Oscar Fish: Unveiling the Threat of Mass Mortality and AMR Dissemination.

The Oscar fish (Astronotus ocellatus) is among the most commonly domesticated and exported ornamental fish species from Kerala. The ornamental fish industry faces a significant challenge with the emergence of diseases caused by multi-drug-resistant bacteria. In the present study, six isolates were resolved from the diseased Oscar fish showing haemorrhages, necrosis, and loss of pigmentation. After phenotypic and genotypic characterization, the bacteria were identified as Edwardsiella tarda, Klebsiella pneumoniae, Enterococcus faecalis, Escherichia coli, Brevibacillus borstelensis, and Staphylococcus hominis. Experimental challenge studies in healthy Oscar fish showed that E. tarda caused 100% mortality within 240h with 6.99 × 106CFU/fish as LD50 and histopathology revealed the typical signs of infection. The pathogen was re-recovered from the moribund fish thereby confirming Koch's postulates. E. tarda was confirmed through the positive amplification of tarda-specific gene and virulence genes viz., etfD and escB were also detected using PCR. Antibiotic susceptibility tests using disc diffusion displayed that the pathogen is multi-drug-resistant towards antibiotics belonging to aminoglycosides, tetracyclines, and quinolones categories with a MAR index of 0.32, which implicated the antibiotic pressure in the farm. Plasmid curing studies showed a paradigm shift in the resistance pattern with MAR index of 0.04, highlighting the resistance genes are plasmid-borne except for the chromosome-borne tetracycline resistance gene (tetA). This study is the first of its kind in detecting mass mortality caused by E. tarda in Oscar fish. Vigilant surveillance and strategic actions are crucial for the precise detection of pathogens and AMR in aquaculture.

Victimological Characteristic of Crimes against Aquatic Biological Resources

The article is a study of the main victimological features of crimes against aquatic biological resources . The author identifies the main groups of people most exposed to the adverse consequences of these crimes. The key criteria for the identification of these groups were the peculiarities of their fisheries management activities, the degree of their material dependence on it and the natural characteristics of the relevant regions. These persons include fish farms, industrial fishing entities, representatives of the indigenous small-numbered peoples of the North, Siberia and the Far East of the Russian Federation and other participants in fisheries relations . Special attention is paid to the damage caused to the interests of the state . A set of measures for victimological prevention of crimes against aquatic biological resources is proposed. These measures should be aimed at the formation of active social behavior of these persons, along with which public authorities and local-self governments, law enforcement agencies, representatives of industry businesses and certain categories of citizens should participate in their implementation . The results are important for the further development of the doctrinal provisions of environmental criminology in terms of issues of environmental victimization, and countering criminal encroachments in the field of fisheries.

Baía dos Tigres - The dynamic of the process

Baía dos Tigres is a well-known sandbank in Angola, referred to since the 15th century when the Portuguese navigators passed there in 1488, on the voyage that allowed them to round the Cape of Good Hope, in southern Africa. It is a phenomenon of a sedimentary nature and therefore it is linked to the availability of solid flow carried by the drift current coming from the South. However, several changes in its condition have been recorded over the centuries. It has shifted from being a sandbank (restinga) and an island, this latter occurring when the isthmus that connects it to the mainland broke, becoming a restinga again when it reestablisheb itself. In March 1962, a huge storm at sea destroyed the fragile structure connecting the land to the coast and since then, precisely 60 years ago, Baía dos Tigres became an island, being separated from the coast in a way that seemed definitive. The observation and analysis of the satellite images used in this work seem to suggest that a setback is beginning to appear, since Baía dos Tigres may be starting to reconnect to the mainland. If this occurs, there is the possibility for Angola to have again a true fish hatchery where countless varieties of fishes and molluscs find unique conditions for spawning. Those partially closed conditions, with waters sufficiently oxygenated to generate excellent biological environment for spawning, are of great importance for the future of fish industry in the country. If those conditions are restored, the zone will be protected from the direct action of storms and the speed of currents, covering an area of more than 250 km2. This article makes an up-date of the situation, based on the analysis of satellite images currently available.

Purification, Characterization and Antifungal Activity of the Aspergillus niveus Chitinase Produced Using Shrimp Shells

Chitinases are biotechnologically relevant enzymes that can be applied in such different sectors as pharmaceutical, food, environmental management, the biocontrol of pests and in the paper and cellulose industry. Microorganisms as filamentous fungi are the most important source of these biomolecules. The fungus Aspergillus niveus produces extracellular chitinase when cultured under submerged fermentation using shrimp shells, a residue generated by the fish industry, as a carbon source, for 96 h at 30 °C and 100 rpm. The particle size and concentration of the shrimp shells affected enzyme production. The chitinase was purified until electrophoretic homogeneity through the use of a Sephadex G-100 chromatographic column. It is a monomeric glycoprotein with a molecular mass of 47 kDa estimated using SDS-PAGE and 49.3 kDa determined using gel filtration. The carbohydrate content was 22.8%. The best temperature and pH for enzyme activity were 65 °C and 6.0, respectively. Approximately 80% of the enzymatic activity was preserved at pH 4.0 and 5.0 for 48 h, and the half-life (t50) was maintained for 48 h at 40 °C. Salts, EDTA and β-mercaptoethanol did not affect chitinase activity significantly, but organic solvents reduced it. The kinetic parameters determined using p-NPGlycNac were Km of 2.67 mmol L−1, Vmax of 12.58 U mg of protein−1, Kcat of 2.47 s−1 and K cat/Km of 0.93 s−1 mmol L−1. The A. niveus chitinase inhibited the growth of all fungal strains used, especially Trichoderma harzianum (MIC = 22.4 μg mL−1) and Penicillium purpurogenum (MIC = 11.2 μg mL−1). The chitinase produced by A. niveus presented interesting characteristics that indicate its potential of application in different areas.

Recent advances of Terminalia catappa and its application in fish culture: A review

AbstractHigh‐intensity aquaculture may cause the aquatic animals to experience elevated stress levels, which in turn will increase their susceptibility to illness and result in substantial losses. Farmers frequently use chemical and antibiotic treatments for fish diseases, which have led to drug‐resistant and hazardous residues in farmed fish and the environment. Consequently, finding substitutes for chemicals and antibiotics is crucial for disease management in aquaculture. Terminalia catappa L. or commonly called Indian almond is well known for its medicinal properties and widely distributed in tropical and subtropical regions. T. catappa contains chemical compounds such as tannin and flavonoid that play an important role in antibacterial, anti‐allergy, anti‐inflammation, and anti‐cancer. Numerous studies have been conducted on leaves, bark, fruits, and seed of T. catappa for their applications, including medicinal, bioremediation (dye absorption), and aquaculture use. Much research can be found on the utilization of T. catappa leaves to improve fish well‐being, mostly in the ornamental fish industry. Extract of T. catappa has been found to improve fish survival, growth rate, inhibit bacterial infection, improve water quality, enhance fish colouration, and boost the immune response. Although T. catappa leaves have undergone substantial research, it has not been systematically described in connection to fish species used in aquaculture. This review will systematically discuss the biotoxicity of T. catappa evaluation of growth‐promoting properties, eggs and larviculture performance, and enhancement of fish resistance to various bacteria, fungi, and parasites. In addition, the article also highlights the future perspectives of using T. catappa to improve fish culture.

MATHEMATICAL PROGRAMMING FOR AN OPTIMISED FISH FEED FORMULATION: TOWARDS SUSTAINABLE RED HYBRID TILAPIA FARMING

Common issues raised in the fish industry are the highly-priced feed production and the sustainability of its ingredients. These issues have also directly impacted the majority of local farmers. Aiming for long-term viability, controlling the feed price is therefore crucial. The selection of ingredients plays an important role in optimising the price of feed. This research, therefore, aims to introduce potential fish feed that optimises the production cost while maintaining the nutrient consumption of the intended species. The feed is formulated using a mathematical model that considers all essential requirements. In this research, the feed formulation was mainly focused on the red hybrid tilapia. Least-cost feed formulations were set out for two main life-cycle stages, juvenile and adult stages, specifying levels of three critical limits: Nutrient requirements of the fish (protein, lipid, carbohydrate, and fibre), cost and nutrient composition of nine commonly used ingredients. AIMMS mathematical software was used to assist the computation to optimality, reducing the cost significantly while providing complete and sufficient nutrients for the fish. From analysing previous research to obtaining advice from experts and demands from industry, 10 different mathematical models were constructed as options to produce potential fish feeds at minimal costs. These models produce feeds that cost less than the commercial pellets available up to 38%. The feed formulation promises cheaper feed and could benefit the industry, indirectly supporting the small-scale local farmers. A thorough discussion of the use of the mathematical model in relation to producing an optimised feed formulation and some managerial insights are included in this research with elaborations on utilising the results to future research opportunities.

Dissertation Title: Challenges in the Processing, Production, and Marketing of the Dried Fish Industry

Dissertation Title: Challenges in the Processing, Production, and Marketing of the Dried Fish Industry

Structure/function relationship of Atlantic bonito (Sarda sarda Bloch) myoglobin and comparison with Atlantic and Tinker mackerel myoglobins

Atlantic bonito meat has economic potential as an alternative to mackerel consumption. Thus, considering the presence of myoglobin (Mb) in red fish muscles, we report the characterisation of Atlantic bonito Mb compared to Atlantic and Tinker mackerel Mbs since this haemoprotein is implicated in lipid oxidation and fish meat preservation. A plethora of biochemical approaches were employed to purified Mb from Atlantic bonito and determine the autoxidation rate constant (0.189 ± 0.009 h−1), melting temperature (Tm = 72.84 ± 1.02 °C) and pseudoperoxidase activity in different conditions (pH and several cations). Atlantic and Tinker mackerel Mbs showed a lower Tm (∼66.85 °C), while oxyMb autoxidation rate constant was higher for Atlantic mackerel (∼1.08-fold) and lower for Tinker mackerel (∼1.35-fold) compared to Atlantic bonito. This Mb had a Michaelis-Menten constant (Km) of 38.63 ± 1.89 μM, ∼2.49-fold and 2.27-fold lower than Atlantic and Tinker mackerel Mbs, respectively. Atlantic bonito Mb primary structure has 146 amino acid residues with the N-terminal acetylated and 25 amino acid substitutions with respect to Atlantic and Tinker mackerel Mbs. In silico analysis revealed that 7 out of 25 substitutions are close to the haem-pocket, while 18 out of 25 are far from this region. All substitutions, except H20, L70 and L81 are exposed on the protein globular surface. Overall, the results of this research provide new information for future studies that will be useful to the fish industry for preservation of frozen or canned Atlantic bonito meat considering the presence of Mb as a reactive haemoprotein.

Does the amount of pacific salmon catch affect the wholesale prices of their products?

Since 2017, volatility of weekly wholesale prices for pacific salmon increased in the Russian domestic market, particularly in the Far East of Russia, that had coincided with higher fluctuations of the weekly and annual catch. Correlation between these variables is investigated for the period from 2010 to 2022, using graphical and regression analyses and 18-factor model for prices of the pink, chum, and sockeye salmon products. The dynamics of prices for pacific salmon products cannot be fully explained only by catch dynamics, but depends on a number of supplementary factors affecting the activities of fish industry enterprises. Anyway, a degree of the catch and price in preceding weeks influence on the wholesale prices is estimated for the main species of pacific salmon. The impact of the catch factor is not significant though more considerable for pink salmon product prices than for the other pacific salmon species.

The Role of Job Satisfaction in Mediating the Workload on Employee Performance in the Canned Fish Industry Sector in Bitung City, North Sulawesi

This research was conducted with the aim of measuring the influence of workload on employee performance through employee job satisfaction in the fish canning industry sector, Bitung City, North Sulawesi. The research population was 2,751 people, and the research sample was 118 respondents, using the Slovin formula. The data analysis techniques used in this research are validity testing, reliability testing, and Structural Equation Modeling (SEM) analysis. The research results show that Workload has a positive and significant effect on Job Satisfaction. Workload has a positive and significant effect on employee performance. Job satisfaction influences employee performance. Furthermore, workload influences employee performance through job satisfaction.

From 7-dehydrocholesterol to vitamin D3: Optimization of UV conversion procedures toward the valorization of fish waste matrices

Vitamin D, a fat-soluble steroid, has increasingly taken a central role due to its crucial role in human health. It is estimated that about 40% of worldwide population are vitamin D deficient. The fish industry produces significant quantities of waste daily, with consequent high environmental impact. The aim of this work is to place a first brick for the fish waste reuse as a source of vitamin D3 extracts to be used for nutraceutical purposes. For this purpose, an UV conversion method for transforming the 7-dehydrocholesterol, highly present in fish, in vitamin D3 has been optimized. The UV wavelength, exposure time, temperature, stirring, and UV intensity were optimized using a surface response design tool. The optimized treatment was applied to five fish species with different fat percentages and the results were very promising reaching vitamin D3 levels >10 times higher than the pre-treatment ones.

The Effect of Low Temperature Storage on the Lipid Quality of Fish, Either Alone or Combined with Alternative Preservation Technologies.

Marine foods are highly perishable products due to their high content of polyunsaturated fatty acids, which can be readily oxidized to form peroxides and secondary oxidation products, thus conferring such foods undesirable organoleptic characteristics and generating harmful compounds that are detrimental to the health of consumers. The use of preservation methods that minimize lipid oxidation is required in the fishing and aquaculture industries. Low temperature storage (chilling or freezing) is one of the most commonly used preservation methods for fish and seafood, although it has been shown that the oxidation of the lipid fraction of such products is partially but not completely inhibited at low temperatures. The extent of lipid oxidation depends on the species and the storage temperature and time, among other factors. This paper reviews the effect of low temperature storage on the lipid quality of fish, either alone or in combination with other preservation techniques. The use of antioxidant additives, high hydrostatic pressure, irradiation, ozonation, ultrasounds, pulsed electric fields, and the design of novel packaging can help preserve chilled or frozen fish products, although further research is needed to develop more efficient fish preservation processes from an economic, nutritional, sensory, and sustainable standpoint.

The effect of various drugs on the survival of sterlet fry in bacterial contamination of water

Currently, aquaculture is a reliable source of increasing the volume of fish food products and serves as a guarantor of food security. The sterlet Acipenser ruthenus L. is one of the main objects of freshwater aquaculture. Feeding with artificial feed, high planting densities and other negative factors of industrial fish farming inevitably lead to increased stress loads. This affects the physiological state of fish. Disturbances in the functioning of the immune system leads to outbreaks of infectious diseases and epizootic disadvantage of pond fish farms. In modern aquaculture, various antibiotics, probiotics and plant preparations are used to treat and prevent bacterial diseases. In this paper, the effect of different feed additives on the survival rate of sterlet fry to infectious agents was investigated. For this purpose, 150 individuals each were seeded into separate pools with high content of myxobacteria and aeromonads in the water microflora. Studies were conducted in 5 separate pools: control group with feed and 4 experimental pools with different feed additives. The duration of the experiment was 75 days. During the experiment, the number of surviving fries was recorded, and at the end, survival rate was calculated as a percentage of the total number of fish initially planted in each pool. Differences in the effect of different feed additives on the survival rate of fry were found. The highest escapement was recorded in the control group and the least in the group with Antibac-100. In the other experimental groups, the percentage of surviving fry was higher than in the control.

Efecto de los ciclos de congelación y descongelación en las propiedades fisicoquímicas, capacidad de retención de agua y la histología de Sardinella aurita

This study investigates the impact of freeze–thaw cycles on samples of Sardinella aurita, focusing on the examination of physicochemical properties, water–holding capacity, color changes, and histological alterations in fish meat. The present findings indicate significant variations in the studied parameters, i.e., pH, water activity, lightness (L*), redness (a*), yellowness (b*), protein solubility (mg·g-1), moisture content (%), thawing loss (%), centrifugation loss (%), cooking loss (%), underscoring the importance of comprehending the consequences of freezing–thawing in the fishing and food processing industry. Initially, a statistically significant decrease in pH levels was observed (T0: 6.23 ± 0.1, T4: 6.19 ± 0.1), followed by a notable increase after the fifth freeze–thaw cycle (T5: 6.47 ± 0.1), possibly due to chemical and microbiological composition shifts. Water activity exhibited a gradual decrease (T0: 0.911 ± 0.009, T4: 0.899 ± 0.01), likely attributed to water loss during freezing–thawing (P<0.05). Colorimetry results demonstrated a significant decrease in brightness (L*) and a slight increase in yellow hue (b*) throughout the cycles, with values ranging from 63.51 (T0) to 33.64 (T5) for L* and from 26.74 (T0) to 17.28 (T5) for b*. These variations highlight notable and significant changes in the product’s color over the freeze–thaw cycles (P<0.05). Histological analysis revealed structural changes, including muscle fiber dehydration. These observed changes hold implications for product quality and consumer perception. It is essential to recognize that various factors, such as fish size, seasonality, and environmental conditions influence these results. Further research is needed to delve deeper into these aspects. In essence, this study offers valuable insights for industry professionals, aiding them in making informed decisions regarding seafood products subjected to freezing–thawing cycles. This not only ensures product quality and safety but also helps prevent food fraud and provides consumers with high–quality products.