Objective: Describe knowledge, attitudes and related factors as well as the readiness regarding opting prenatal screening tests of first-trimester pregnant women in North Tu Liem District, Hanoi in 2022. Analyzing some factors affect knowledge and attitudes toward the use of prenatal screening tests. Methods: A quantitative, cross-sectional study was applied, using a 5-point Likert scale to assess. SPSS20 software was used to analyzed the answers of 150 participants. Main findings: The mean score of knowledge and attitudes ranged from 3,47 – 3,69 and 3,34 – 3,57 respectively on a 5-point Likert scale. In addition, Ultrasound was the most well-known test (100% women knew about it) and they mainly accessed prenatal screening’s information through Obstetricians (119/150 options). Notably, educational level, employment status and place of residence significantly affected knowledge and attitudes toward opting prenatal screening tests while the number of children was unrelated. Conclusions: First-trimester pregnant women in North Tu Liem District, Hanoi had a good level of knowledge and relatively positive attitudes toward the use of prenatal screening tests. Pregnant women with a high level of education, having an occupation and living in the urban areas have better knowledge and attitudes toward using prenatal screening tests. Keywords: Assessment, knowledge, attitudes, pregnant women, prenatal screening tests, first-trimester, North Tu Liem.