The Brazilian Cerrado is the second largest biome in Brazil, and its natural landscapes have undergone transformations, due in large part to anthropic activities. However, little is known about the effects of these activities on the natural regeneration of Cerrado vegetation. Thus, the objective of this study was to evaluate the accumulation of woody biomass in an area of a Cerrado sensu stricto that was monitored for 27 years after the implementation of six silvicultural systems.A period of 17 years was sufficient for the woody community of the Cerrado sensu stricto to naturally recover its original stock of aerial biomass after the application of silvicultural systems. A Few woody species contribute to the total aerial biomass accumulated over time, and such contribution is variable with time and with the type of silvicultural system.At 27 years after cutting, the largest fraction of biomass accumulated in the area were represented by the species Miconia leucocarpa, Blepharocalyx salicifolius, Schefflera macrocarpa, Qualea grandiflora, Dalbergia miscolobium, Eriotheca pubescens, Qualea parviflora and Caryocar Brasiliense, which also demonstrated higher fire resistance.A period of 17 years after the implementation of silvicultural systems is considered sufficient for an area to recover naturally to the original stock of woody biomass at the community level. This result causes a greater reduction in the biomass of fire-sensitive species and transforms a Cerrado from a carbon sink into a source of CO2 emissions to the atmosphere.