Background: Fire safety is the set of practices intended to prevent the destruction caused by Fire. Fire safety is the set of practices intended to reduce destruction caused by fire. Fire safety measures include those that are intended to prevent the ignition of an uncontrolled fire and those that are used to limit the spread and impact of a fire.[4] Fire related accidents have, on average, killed 35 people every day over the five years between 2016 and 2020, according to a report by the National Crime Records Bureau (NCRB). Aims and Objectives: To Assess the knowledge regarding Fire Safety and their practices among the students of 3rd year B.Sc. Nursing, Ambaji Nursing College, Ganeshpura, and to find out Association between selected demographic variable and to develop on hand training module. Materials and Method: The study was conducted among 60 students of Ambaji Nursing College, Ganeshpura and using simple random sampling technique. Data were obtained using structured knowledge questionnaire to assess the knowledge regarding Fire safety and their practices. Descriptive and Inferential statistics were use to analyze the Demographic data and used structured questionnaire. Karl person formula is used to determine Correlation whereas the Association is determined by using Chi -Square formula. Results and Conclusion: The study revealed that majority of students level of knowledge are 36.70% poor knowledge, 61.70% students are average knowledge and 1.70% students are good knowledge regarding fire safety and their practices. There exist positive moderate correlation (r = 0.75) between knowledge and their practices. Study also revealed there is no significant Association between the knowledge and their demographic variables such as Age, Gender, Annual household income, Religion and attending any training of fire safety practices at p ≤ 0.05 level.