Humans primarily alter fire regimes in three ways: changing the distribution and density of ignitions, shifting the seasonality of burning, or altering available fuels. Remote sensing techniques are a highly feasible and effective tool for monitoring wildfires, delineating burn perimeters, describing patterns of fire occurrence, characterizing the degree of intensity, or fire risk mapping. In this study MODIS and Sentinel-2 satellite images were used to analyze the burned areas in the Luhansk and Donetsk Regions of Ukraine (2006–2022), and specifically how the political and military hostilities in 2014 impacted fire activity. The burned areas detected by the MODIS sensor were selected using a size (32 ha) and temporal (May–October) filter. The Relativized Burn Ratio (RBR) index was used to identify burned areas, while the Normalized Difference Water Index (NDWI) was employed to discriminate the water bodies in Sentinel-2 images. We analyzed the burned area data over the 17-year period, eight of them during the years before the conflict and the other nine after the conflict. The results show that burned area has increased from the pre-conflict period (2008–2013) by 689,898 ha to the post-conflict period (2014–2022) around the contact line. In addition, the frequency of fires has increased in the post-war period, especially towards the south of Donetsk. Finally, Sentinel-2 imagery was used to map Large Wildfires (LAWF), which means fires with a burned area >500 ha. Our study concluded that MODIS MCD64A1 underestimates the burned area with a recorded difference of up to 40% of the area in one of the analyzed fires. In addition, an analysis of the burned areas classified according to the main land use has been performed for both sensors. The results obtained show a regression for the total area of R2 = 0,97 and both sensors show high correlation between them for each land use analyzed. Grasslands and agricultural land covers were most affected by LAWFs during the conflict period (2014–2022), noting that fires near urban entities were also more frequent in the war period.