This paper presents design, numerical analysis and experimental investigation of an origami inspired X-band accordion waveguide that is aimed to create a flexible and adaptive solutions. The design process is conducted in a Finite Integration Technique (FIT) based microwave simulation software to model and simulate the proposed waveguide, and that shows structural benefits of origami technique based paper folding. Proposed waveguide model is numerically analyzed under both X and Y bending with 30˚, 60˚, 90˚ and 120˚ bending angle to provide its flexibility. Besides, the electric and magnetic field distributions were also obtained and explained in details to provide working mechanism of the proposed accordion waveguide. The S21 transmission characteristics between ports exhibits a decrease of 0.015 dB in numerical results, which is negligible and acceptable in applications. The experimental results demonstrate that the proposed waveguide has effective transmission characteristics within the 8–12 GHz X-band frequency range, with a satisfactory S21 transmission parameter. The numerical and experimental results show that proposed origami inspired accordion waveguide has a huge potential for application in dynamic microwave and satellite components.